全文获取类型
收费全文 | 1043篇 |
免费 | 88篇 |
国内免费 | 13篇 |
专业分类
耳鼻咽喉 | 9篇 |
儿科学 | 37篇 |
妇产科学 | 81篇 |
基础医学 | 129篇 |
口腔科学 | 12篇 |
临床医学 | 132篇 |
内科学 | 247篇 |
皮肤病学 | 32篇 |
神经病学 | 37篇 |
特种医学 | 81篇 |
外科学 | 111篇 |
综合类 | 32篇 |
一般理论 | 4篇 |
预防医学 | 115篇 |
眼科学 | 6篇 |
药学 | 28篇 |
肿瘤学 | 51篇 |
出版年
2023年 | 8篇 |
2021年 | 20篇 |
2020年 | 13篇 |
2019年 | 18篇 |
2018年 | 20篇 |
2017年 | 14篇 |
2016年 | 13篇 |
2015年 | 10篇 |
2014年 | 29篇 |
2013年 | 43篇 |
2012年 | 41篇 |
2011年 | 41篇 |
2010年 | 37篇 |
2009年 | 51篇 |
2008年 | 40篇 |
2007年 | 38篇 |
2006年 | 46篇 |
2005年 | 44篇 |
2004年 | 39篇 |
2003年 | 30篇 |
2002年 | 35篇 |
2001年 | 27篇 |
2000年 | 28篇 |
1999年 | 22篇 |
1998年 | 22篇 |
1997年 | 27篇 |
1996年 | 26篇 |
1995年 | 25篇 |
1994年 | 15篇 |
1993年 | 23篇 |
1992年 | 22篇 |
1991年 | 14篇 |
1990年 | 23篇 |
1989年 | 26篇 |
1988年 | 17篇 |
1987年 | 12篇 |
1986年 | 19篇 |
1985年 | 17篇 |
1984年 | 13篇 |
1983年 | 18篇 |
1982年 | 6篇 |
1981年 | 10篇 |
1980年 | 7篇 |
1979年 | 13篇 |
1978年 | 13篇 |
1977年 | 8篇 |
1974年 | 9篇 |
1973年 | 7篇 |
1972年 | 5篇 |
1970年 | 10篇 |
排序方式: 共有1144条查询结果,搜索用时 15 毫秒
1.
The difficulty of diagnosing NCSE in clinical practice; external validation of the Salzburg criteria
Rianne J. M. Goselink Jeroen J. van Dillen Marjolein Aerts Johan Arends Charlotte van Asch Inge van der Linden Jaco Pasman Christiaan G. J. Saris Machiel Zwarts Nens van Alfen 《Epilepsia》2019,60(8):e88-e92
To improve the diagnostic accuracy of electroencephalography (EEG) criteria for nonconvulsive status epilepticus (NCSE), external validation of the recently proposed Salzburg criteria is paramount. We performed an external, retrospective, diagnostic accuracy study of the Salzburg criteria, using EEG recordings from patients with and without a clinical suspicion of having NCSE. Of the 191 EEG recordings, 12 (12%) was classified as an NCSE according to the reference standard. In the validation cohort, sensitivity was 67% and specificity was 89%. The positive predictive value was 47% and the negative predictive value was 95%. Ten patients in the control group (n = 93) were false positive, resulting in a specificity of 89.2%. The interrater agreement between the reference standards and between the scorers of the Salzburg criteria was moderate; disagreement occurred mainly in patients with an epileptic encephalopathy. The Salzburg criteria showed a lower diagnostic accuracy in our external validation study than in the original design, suggesting that they cannot replace the current practice of careful weighing of both clinical and EEG information on an individual basis. 相似文献
2.
神经生长因子对小鼠突触体内Ca^2+水平的调节作用 总被引:4,自引:1,他引:3
观察了多次海马内微注射NGF对小鼠突触体内游离钙水平的影响,并在离体情况下观察NGF对EGTA和CaCl2分别造成突触体内低钙和高钙状态的调节作用。结果如下:(1)在体实验表明,一定剂量的NGF可显著降低老年小鼠海马突触体内游离钙水平(P<005);(2)离体实验表明,当突触体游离钙水平降低时,适当剂量的NGF具有升高游离钙水平的作用;而突触体内游离钙水平升高时,则NGF有降低游离钙水平的作用。提示NGF对游离钙水平的双向调节作用可能是NGF改善老年性记忆衰退的作用机制。 相似文献
3.
4.
Laparoscopic cryosurgery for hepatic tumors 总被引:2,自引:0,他引:2
Background: Hepatic cryosurgery has been shown to be a safe technique that may be well suited to a laparoscopic approach.
Methods: The technical feasibility and safety of laparoscopic cryosurgery was explored first in a pig model. Thereafter we performed
the first successful case of laparoscopic hepatic cryosurgery at our institution.
Results: In the animal model, we found that it is possible to safely identify, target, and cryoablate specific lesions in the liver.
Temperature in the peritoneal cavity remained above 35°C, and pathologic examination of the abdominal wall around the cryoprobe
site revealed no damage. We also successfully treated a 62-year-old man with a metastatic colorectal carcinoma deep in the
right lobe of the liver with laparoscopic cryosurgery using a transpleural approach.
Conclusion: We conclude that laparoscopic cryosurgery is feasible for lesions anywhere in the liver. For lesions high on the dome of
the liver, a transpleural approach may provide better access. 相似文献
5.
6.
Bonnie B. Asch Harold L. Asch Daniel L. Stoler Garth R. Anderson 《International journal of cancer. Journal international du cancer》1993,54(5):813-819
Of the several families of endogenous retrovirus-like elements present in the mouse genome, only mouse mammary tumor virus has been analyzed for its role in mammary carcino-genesis. Very little is known about the expression and activities of other retro-elements in normal and malignant mammary epithelium. We have begun investigating the possible involvement of the 3 retrotransposons, intracisternal A particles (IAPs), murine-leukemia-virus-related (MuLVr) elements, and VL30 sequences, in neoplastic progression of the mammary gland in BALB/c mice. The purpose of the present study was to determine which of these elements was active in primary mammary carcinomas induced by chemical, hormonal and viral agents. Each of these cancers had aberrant expression of at least one of the latter retrovirus-like components. IAP and/or MuLVr sequences were over-expressed 3 to 100-fold in most of the tumors as compared with normal mammary tissue, whereas VL30 expression was markedly decreased by 5- to 35-fold in almost all of the neoplasms. Our results thus demonstrate that substantial changes in the expression of one or more of these 3 families of endogenous retrotransposons are triggered during mouse mammary tumorigenesis, regardless of etiology. Direct involvement of lAPs and MuLVr elements in neoplastic progression by transposition and insertional mutagenesis in the genome of several hematopoietic cell types has already been demonstrated. Their elevated expression in many mammary carcinomas suggests that these retrotransposons may also be potential participants in some pathways of mouse mammary carcinogenesis. 相似文献
7.
MY Mancao LJ Sindel PH Richardson FM Silver 《Acta paediatrica (Oslo, Norway : 1992)》1996,85(1):118-120
Croup is an acute infectious illness usually occurring in children; it is characterized by brassy cough and stridor. The main pathogens include mainly parainfluenza and influenza viruses. Recently there have been reports of prolonged croup caused by the herpes simplex viruses. We report two cases of prolonged croup due to herpes simplex types 1 and 2. We also review and summarize the reported pediatric cases of herpetic croup. 相似文献
8.
Korthuis PT Asch SM Anaya HD Morgenstern H Goetz MB Yano EM Rubenstein LV Lee ML Bozzette SA 《Journal of acquired immune deficiency syndromes (1999)》2004,35(3):253-260
BACKGROUND: Lipid screening is recommended for patients taking protease inhibitors (PIs). METHODS: We examined data from the Veterans Administration Immunology Case Registry to assess lipid screening among HIV-infected veterans who received PIs for at least 6 consecutive months during 1999 and 2001. We estimated crude and adjusted associations between lipid screening and patient characteristics (age, gender, HIV exposure, and race/ethnicity), comorbidities (AIDS, cardiovascular disease, diabetes, hypertension, smoking, and hyperlipidemia), and facility characteristics (urban location, case management, guidelines, and quality improvement programs). RESULTS: Among 4065 patients on PIs, clinicians screened 2395 (59%) for lipids within 6 months of initiating treatment. Adjusting for patient characteristics, comorbidities, facility traits, and clustering, lipid screening was more common among patients who were cared for in urban areas (relative risk [RR] = 1.3, confidence limits: 1.0-1.5), diabetic (RR = 1.2, confidence limits: 1.1-1.3), or previously hyperlipidemic (RR = 1.4, confidence limits: 1.3-1.5) and less common among patients with a history of intravenous drug use (IVDU) (RR = 0.90, confidence limits: 0.79-1.0) or unknown HIV risk (RR = 0.85, confidence limits: 0.75-0.95). CONCLUSIONS: Six in 10 patients taking PIs receive lipid screening within 6 months of PI use. Systemic interventions to improve overall HIV quality of care should also address lipid screening, particularly among patients with unknown or IVDU HIV risk and those cared for in nonurban areas. 相似文献
9.
Duncan WC; Illingworth PJ; Young FM; Fraser HM 《Human reproduction (Oxford, England)》1998,13(9):2532-2540
The molecular mechanisms involved in luteolysis are still unclear in the
primate. This study aimed to investigate the effect of induced luteolysis
on the ovarian luteinizing hormone (LH) receptor and the steroidogenic
enzyme, 3beta-hydroxysteroid dehydrogenase (3beta-HSD) in the marmoset
monkey. Luteolysis was induced in the mid-luteal phase either directly by
systemic prostaglandin F2alpha (PGF2alpha), or indirectly by LH withdrawal
using systemic gonadotrophin releasing hormone antagonist (GnRHant)
treatment. The LH receptor was studied by isotopic mRNA in-situ
hybridization and in-situ ligand binding and 3beta-HSD expression was
studied using isotopic mRNA in-situ hybridization and immunohistochemistry.
Induced luteolysis was associated with a reduction in the expression of LH
receptor (P < 0.0001) and 3beta-HSD mRNA, closely followed by a
reduction in the LH receptor (P < 0.05) and 3beta-HSD protein
concentrations within 24 h. There were no differences in the findings
whether luteolysis was induced with PGF2alpha or GnRHant. This study shows
that disparate mechanisms to induce luteolysis in the primate result in an
identical rapid loss of the LH receptor and 3beta-HSD. In conclusion,
induced luteolysis leads to rapid loss of the steroidogenic pathway in
luteal cells.
相似文献
10.