针刺加TDP照射治疗痛风30例疗效观察

痛风性关节炎是嘌呤代谢紊乱所致的疾病。本病好发于中老年患者．以男性为多起病急，一般为单个关节发炎，特别是第一趾关节最多累及。病初剧痛，局限于短时间内高度肿胀、灼热．皮肤紫红发亮。近几年来.笔者应用针灸结合TDP照射治疗痛风性关节炎30例．收到了满意疗效。兹将施治情况报告如下。1临床资料1．1一般资料：本组30例中，均为门诊患者．男27例．女3例：年龄最小3O岁．年龄最大57岁；病程3～10日不等，病程较长者均经其它疗法治疗无效来求治者。1．2诊断标准：典型临床表现，尤其是中老年男性患者突发性姆趾，路路，踝等处单个关…     

对玉竹含量测定实验进行探究式教学的尝试

以玉竹多糖的含量测定为例,改变以往按照《中国药典》(一部)固定步骤的做法,在实验中采用两种提取方法和两种比色方法对玉竹多糖的含量进行测定,比较不同的方法对含量测定结果的影响,以提高学生的综合实验能力和科研素质。结果表明:改进后的实验方案能够提高学生的积极性、创新能力和科研能力,并且有利于学生责任感及团结协作精神的培养,取得了很好的教学、育人效果。     

细胞因子治疗4.5Gyγ射线照射比格犬造血损伤的机制初探

目的 探讨多种细胞因子配伍应用治疗4.5 Gy γ射线照射比格犬造血系统损伤的作用及可能机制。方法 16只比格犬均给予4.5 Gy ⁶⁰Co γ射线全身照射,随机分为照射对照、综合对症和细胞因子3组。细胞因子组在综合对症支持治疗的基础上应用rhG-CSF、rhIL-11和rhIL-2联合治疗,采用流式细胞术检测外周血中CD34+细胞含量、有核细胞周期及凋亡比例。结果 4.5 Gy γ射线照射犬外周血中CD34+细胞含量在照射后1 d明显下降(照射对照组和综合对症组分别为照前值的61.3%和52.1%),G₀/G₁期有核细胞比例增加(分别为99.27%和99.49%),且凋亡率(分别为26.93%和21.29%)和坏死率(分别为3.27%和4.14%)明显升高(与照前值比较, P<0.05);而经过细胞因子治疗后,外周血中CD34+细胞含量在照射后1 d即明显升高(为照前值的135.6%),G₀/G₁期有核细胞比例(99.71%)进一步增加,其凋亡率(5.66%)和坏死率(1.60%)明显低于照射对照和综合对症组。结论 本研究的细胞因子组合可能通过动员骨髓中CD34+细胞到外周血,使细胞周期阻滞在G₀/G₁期,减少细胞凋亡,从而促进极重度骨髓型急性放射病犬造血功能的恢复。     

Therapeutic effects of combined cytokines on hematopoietic injuries induced by 4.5 Gy γ-rays irradiation in beagles

Objectivc To observe the therapeutic effects of combined cytokines on hematopoietic injuries induced by 4.5 Gy60 Co γ-rays irradiation in beagles,and to provide experimental evidences for the clinical treatment of extremely severe myeloid acute radiation sickness(ARS).Methods 16 beagles were given 4.5 Gy60 Co γ-rays total body irradiation,and then randomly assigned into irradiation control group,supportive care group and cytokines group.In addition to supportive care,recombinant human granulocyte colony-stimulating factor (rhG-CSF),recombinant human interleukin-11(rhIL-11)and recombinant human interleukin-2(rhIL-2)were administered subcutaneouly to dogs in cytokines group.Peripheral blood hemogram was examined once every two days.Bone marrow and peripheral blood were collected to proceed colony cultivation 4 d pre-irradiation and 1 and 45 d post-irradiation.Conventional histopathological sections of sternum were prepared to observe the histomorphology changes. Results After irradiation,the population of all kinds of cells in peripheral blood declined sharply.WBC nadir Was elevated(1.04×109/L,but 0.28×109/L and 0.68×109/L for the irradiation control group and the supportive care group separately),the duration of thrombocytopenia was shortened (24 days,but 33 days for the supportive care groug) and red blood cell counts were maintained in the range of normal values after cytokincs treatment in combination.The colony forming efficiency of haemopoietic stem cells(HSCs)in bone marrow and peripheral blood decreased obviously 1 d post irradiation,but recovered to the level of that before irradiation 45 d post irradiation after supportive care and cytokines treatment.Hematopoietic cells disappeared in bone marrow of animals in irradiation control group,but hematopoietic functions were recovered after cytokines were administrated.Conclusions RhG-CSF.rhIL-11 and rhIL-2 used in combination could elevate WBC nadir,accelerate the recovery of leukocytes,platelets and red blood cells and promote the proliferation,differentiation and maturity of HSPCs left in the body after 4.5 Gy γ-rays total body irradiation,eventually restore the hematopoietic function.Hence,combination of rhG-CSF,rhIL-11 and rhIL-2 could serve as better therapeutic strategy to treat extremely severe myeloid ARS.     

环椎轴位的摄影方法

环椎正侧位为我们日常投照工作中的常规位置,是检查环枢椎关节脱位、齿状突骨折及骨质改变必不可少的方法。而对环椎后弓骨折、移位就难以确诊。笔者结合人体标本和对患者进行投照,总结出环椎轴位的投照方法。方法　患者俯卧于摄影台上,两臂放于身旁,头颅后仰,使作者单位:261031潍坊医学院附属医院放射科(王林、郭新全、张始状、曹贵文、张益海);眼科(王金香)头颅冠状面与床面呈75°。球管向足侧倾斜15°,暗盒置于下颌颏下三角区。中心线对准枕骨粗隆上方3ｃｍ处。环椎轴位像投照在颏下三角区和下颌下三角区内。讨论　环椎轴位片与ＣＴ片对…     

Experimental therapeutic effect of combined cytokines on beagle dogs exposed to 4.5 Gy γ-rays

Objective To evaluate the effects of combined administration of recombinant human interleukin-11(rhIL-11),recombinant human G-CSF(rhG-CSF)and recombinant human interleukin-2 (rhIL-2)on acute radiation sickness(ARS)beagles.Methods Sixteen beagle were irradiated with 4.5 Gy60 Co γ-rays to establish ARS models,and were divided into irradiation control group,supportive care group and combined cytokines treatment group.After irradiation irradiation control group was given no treatment,the dogs in supportive care group received purely symptomatic treatment,while combined cytokines treatment group received rhIL-11 50μg/(kg·d)and rbG-CSF 10μg/(kg·d)subcutaneously(0-14 d)and rhIL-2 1×1 06 U/d(29-43 d)besides symptomatic treatment.Manifestation and characteristics of ARS beagles were observed,and the survival time were recorded.At last,post-mortem examination and histological examination were performed.Results All animals underwent nausea,diarrhea and fever.After irradiation,all animals in irradiation control group died in two weeks,and the mean survival time was 12.7 d,while only one died at 33 d in supportive care group.All dogs in combined cytokine group survived at 45 day after exposure,and their haematopoiesis and gastrointestinal tract were recovered.Conclusions Combination of rhIL-11 + rhG-CSF + rhIL-2 treatment could be significantly effective on ARS beagles irradiated by 4.5 Gy60 Co γ-rays,which could accelerate injured haemotopoiesis and intestinal tract recovery,increase the survival rate and improve the life quality of animals.     

Clotting mechanism in beagles irradiated by 4.5 Gy γ-rays

Objective To explore the clotting mechanism in beagles irradiated by 4.5 Gy γ-rays after treatment with supportive care,or supportive care and combined cytokines.Methods Sixteen beagles were divided into irradiation control group,Supportive care group and combined cytokines treatment group.Platelet aggregation test,thrombelagtography (TEG) and the time measurement were analyzed in vitro.Results In irradiation group and supportive care group,the platelet aggregation rates in beagles were decreased markedly and the k value of TEG was increased 7 d post-irradiation,while those indexes in combined cytokines treatment group changed little.At 14 d post-irradiation,each parameter of TEG in irradiated group changed obviously.The values of r,k,r+k and M were elevated significantly,clotting time and the maximum coagulation time of thrombus delayed,the Ma value was decreased markedly,and the maximum elasticity amplitude of thrombus was diminished.All parameters in combined cytokines treatment group were better than those in supportive care group.The thrombin time was prolonged obviously in irradiated group 14 d post-irradiation,while the thrombin time was the longest at 2-3 weeks post irradiation in supportive care group and combined cytokines treatment group(P>0.05).Conclusions Cytokines could improve the platelet aggregation and the blood clotting functions of beagles suffering from acute radiation sickness.     

X射线骨矿密度定量分析系统对人体前臂骨矿密度的定量分析方法

依据人体骨骼骨密度的增减,在Ｘ射线机照射下,该骨骼的衰减系数会相应地出现有规律的变化这一现象,现将Ｘ射线骨矿密度定量分析系统定量分析方法报告如下。技术与方法１标准铝楔的选用人体骨的矿物质含量主要是羟磷灰石含量,其密度为２７～３０ｇ／ｃｍ３,平均...     

中药治疗输卵管阻塞不孕16例

<正>凡婚后未避孕,有正常性生活,同居2年而未妊娠者为不孕症。我们用中药灌肠液联合杨氏消炎止痛贴外敷治疗输卵管阻塞不孕16例疗效满意,现报道如下。1临床资料16例均排除男方不孕因素,均无盆腔结核病史,皆为盆腔炎症导致输卵管阻塞而致不孕。年龄23～25岁1例,25～30岁12例,30～35岁2例。结婚2年未孕4例,5年未孕10例。曾有过妊娠而后未避