大鼠脂肪干细胞复合胶原-壳聚糖-硫酸软骨素三维支架构建组织工程软骨

Objective To evaluate the character of the collagen-chitosan-chondroitin sulfate scaffold seeded with rat adipose tissue-derived stromal cells. Methods A dipose tissue were harvested from 6 weeks old Wistar rats and the stromal cells were harvested by type Ⅰ collagenase and then cultured in vitro. Type Ⅰ collagen was fully mixed with chitosan, freeze-dried and cross-linked with chondroitin sulfate, then freeze-dried again and sterilized by ethylene oxide. The pore diameter, water content, porosity of the scaffold were tested. The adipose tissue-derived stromal cells were digested, seeded into the plates, scaffold, and cen-trifuged into pellet, and then induced into cartilage. MTT detection for cell proliferation was done. After 3 weeks, the cell morphology, and cell proliferation and adhesion were observed, and chondrngenic differenti-ation was also analyzed. Results The pore diameter, water content, porosity tested for the scaffold showed an appropriate form. Cell proliferation showed faster in the scaffold and pellet culture system after 5 day, there was still cell proliferation in the scaffold system after 14 days but no obvious changes in the pellet cul-ture system; ceils on the scaffold proliferated densely showed by histological staining, but there was a scaf-fold structure residues in the inner layer. The finding of type Ⅱ immunohistochemistry stain showed that cells express strong positive for type Ⅱ collagen in the scaffold and pellet culture system whereas it was weakly positive in the plate culture system; the specific mRNA for cartilage, type Ⅱ collagen, aggrecan and SOX-9 were expressed in all three systems showed by RT-PCR, but type X collagen was expressed continu-ously in the plate culture system and expressed after 21 days in the pellet culture system, whereas it was not detected in the collagen-chitosan-chondroitin sulfate scaffold system. Conclusion The parameters of the collagen-chitosan-chondroitin sulfate scaffold were suitable in our study. The results suggested that it can promote the adipose tissue-derived stromal cells proliferation and chondrogenic differentiation better than the plate and pellet culture systems and maintain the phenotype of chondrocytes well; it is the optimal choice for cartilage tissue engineering in the future.     

大鼠脂肪干细胞复合胶原-壳聚糖-硫酸软骨素三维支架构建组织工程软骨

Objective To evaluate the character of the collagen-chitosan-chondroitin sulfate scaffold seeded with rat adipose tissue-derived stromal cells. Methods A dipose tissue were harvested from 6 weeks old Wistar rats and the stromal cells were harvested by type Ⅰ collagenase and then cultured in vitro. Type Ⅰ collagen was fully mixed with chitosan, freeze-dried and cross-linked with chondroitin sulfate, then freeze-dried again and sterilized by ethylene oxide. The pore diameter, water content, porosity of the scaffold were tested. The adipose tissue-derived stromal cells were digested, seeded into the plates, scaffold, and cen-trifuged into pellet, and then induced into cartilage. MTT detection for cell proliferation was done. After 3 weeks, the cell morphology, and cell proliferation and adhesion were observed, and chondrngenic differenti-ation was also analyzed. Results The pore diameter, water content, porosity tested for the scaffold showed an appropriate form. Cell proliferation showed faster in the scaffold and pellet culture system after 5 day, there was still cell proliferation in the scaffold system after 14 days but no obvious changes in the pellet cul-ture system; ceils on the scaffold proliferated densely showed by histological staining, but there was a scaf-fold structure residues in the inner layer. The finding of type Ⅱ immunohistochemistry stain showed that cells express strong positive for type Ⅱ collagen in the scaffold and pellet culture system whereas it was weakly positive in the plate culture system; the specific mRNA for cartilage, type Ⅱ collagen, aggrecan and SOX-9 were expressed in all three systems showed by RT-PCR, but type X collagen was expressed continu-ously in the plate culture system and expressed after 21 days in the pellet culture system, whereas it was not detected in the collagen-chitosan-chondroitin sulfate scaffold system. Conclusion The parameters of the collagen-chitosan-chondroitin sulfate scaffold were suitable in our study. The results suggested that it can promote the adipose tissue-derived stromal cells proliferation and chondrogenic differentiation better than the plate and pellet culture systems and maintain the phenotype of chondrocytes well; it is the optimal choice for cartilage tissue engineering in the future.     

后路微创全髋关节置换术早期体会

报道后路小切口微创单侧全髋关节置换术15例。后路小切口微创全髋置换术具有创伤小、出血少、恢复快等优点，但要有严格的手术指征、熟练的操作技术和专用的器械。     

颈椎前路单节段减压四种不同融合方式的疗效比较

目的比较颈椎前路单节段减压并不同融合方法治疗脊髓型颈椎病（cervical spondylotic myelopathy,CSM）的临床疗效。方法对168例单节段病变的CSM患者采用前路减压并植骨融合治疗,其中单纯植骨融合42例（A组）、颈椎前路减压界面固定术（cervical interbody fusioncage,CIFC）54例（B组）、植骨融合并颈椎前路钢板内固定40例（C组）、CIFC并前路钢板内固定32例（D组）。术后定期随访并摄X线片,观察疗效、椎间高度、颈椎前弯曲度和融合情况。结果经过随访,A组融合率为83．3％,B组融合率为96．3％,C组融合率为95．0％,D组融合率为96.9％。终访时,A组平均椎间高度和颈椎前弯曲度较术后早期显著降低（P〈0．05）,B组、C组和D组则无显著差异（P〉0．05）。结论脊髓型颈椎病的治疗关键不仅在于充分减压及有效植骨融合,而且不同融合技术对疗效有明显影响。     

扶正益津汤对放疗所致免疫功能抑制小鼠细胞毒功能的影响

目的观察扶正益津汤对放疗所致免疫功能抑制小鼠细胞毒功能的影响。方法以小鼠腹腔巨噬细胞介导的肿瘤细胞溶解作用(MTC)和抗体依赖细胞介导的细胞毒作用(ADCC)为指标,观察中药扶正益津汤对小鼠巨噬细胞细胞毒活性的影响。结果扶正益津汤对受X射线照射所致免疫功能抑制小鼠细胞毒活性有显著增强作用。结论扶正益津汤可用于放疗辅助用药。     

小儿腓骨小细胞性骨肉瘤一例

小细胞性骨肉瘤(small cell osteosarcoma,SCOC)是一种非常罕见的骨肉瘤,约占骨肉瘤的1.3％～4％.发病年龄与普通骨肉瘤相似,多发生在10～20岁[1,2].该病于1979年由Sim等[3]首次发现.这种小细胞骨肉瘤组织学形态类似于尤文氏肉瘤,预后比传统的骨肉瘤更差.其与尤文氏肉瘤的区别在于二...     

经椎间孔椎体间融合与后路椎体间融合治疗腰椎退行性疾病的疗效比较

<正>本文回顾性分析了我院2010年7月至2011年5月行后路椎体间融合术(PLIF)或经椎间孔椎体间融合术(TLIF)治疗腰椎退变性疾病(DLD)112例,并对2种术式的疗效进行比较研究,现报告如下。1资料与方法1.1一般资料     

同种异体骨移植与自体骨移植修复四肢粉碎性骨折:骨性愈合及骨活性比较

背景:骨移植是一种治疗四肢粉碎性骨折常用的方法,并且可以选择同种异体骨移植或者自体骨移植,两种骨移植方法各有优缺点。目的:对比同种异体骨移植与自体骨移植修复四肢粉碎性骨折在骨性愈合及骨活性方面的临床效果。方法:回顾性分析49例四肢粉碎性骨折患者的临床资料,按照骨移植方法分为观察组与对照组,观察组采用同种异体骨移植修复,对照组采用自体骨移植修复。观察两组骨折临床愈合时间、骨性愈合时间及骨特异性碱性磷酸酶活性,并进行比较。结果与结论:观察组骨折愈合时间及骨性愈合时间分别为(5.8±1.2)周、(5.8±1.5)个月,对照组骨折愈合时间及骨性愈合时间分别为(6.1±1.3)周、(6.1±0.8)个月,两组间骨折愈合时间及骨性愈合时间比较差异无显著性意义(P>0.05);观察组与对照组骨特异性碱性磷酸酶活性分别为(10.45±1.53),(13.58±1.69)μg/L,两组间比较差异有显著性意义(P<0.05)。表明同种异体骨移植与自体骨移植修复四肢粉碎性骨折均可获得良好的临床疗效,但自体骨移植在促进骨骼愈合能力方面有一定的优势。     

胃窗－85超声显像剂在胆总管下段梗阻性病变中的应用

超声对胆总管下段病变的检查, 往往因胃肠道气体的干扰而难以显示。1988年以来, 我们应用胃窗一85超声显像剂充盈胃与十二指肠, 形成一种“前声窗”后对胆总管下段病变进行超声检查, 获得良好效果。