全文获取类型
收费全文 | 891篇 |
免费 | 46篇 |
国内免费 | 6篇 |
专业分类
耳鼻咽喉 | 5篇 |
儿科学 | 41篇 |
妇产科学 | 3篇 |
基础医学 | 146篇 |
口腔科学 | 8篇 |
临床医学 | 57篇 |
内科学 | 208篇 |
皮肤病学 | 19篇 |
神经病学 | 155篇 |
特种医学 | 49篇 |
外科学 | 134篇 |
综合类 | 3篇 |
预防医学 | 8篇 |
眼科学 | 15篇 |
药学 | 28篇 |
中国医学 | 2篇 |
肿瘤学 | 62篇 |
出版年
2022年 | 9篇 |
2021年 | 9篇 |
2020年 | 9篇 |
2019年 | 7篇 |
2018年 | 15篇 |
2017年 | 14篇 |
2016年 | 10篇 |
2015年 | 19篇 |
2014年 | 33篇 |
2013年 | 33篇 |
2012年 | 49篇 |
2011年 | 47篇 |
2010年 | 23篇 |
2009年 | 16篇 |
2008年 | 38篇 |
2007年 | 44篇 |
2006年 | 50篇 |
2005年 | 48篇 |
2004年 | 56篇 |
2003年 | 50篇 |
2002年 | 53篇 |
2001年 | 27篇 |
2000年 | 11篇 |
1999年 | 31篇 |
1998年 | 17篇 |
1997年 | 13篇 |
1996年 | 10篇 |
1995年 | 8篇 |
1994年 | 13篇 |
1993年 | 11篇 |
1992年 | 20篇 |
1991年 | 16篇 |
1990年 | 10篇 |
1989年 | 17篇 |
1988年 | 9篇 |
1987年 | 12篇 |
1986年 | 9篇 |
1985年 | 5篇 |
1984年 | 7篇 |
1983年 | 3篇 |
1982年 | 6篇 |
1981年 | 6篇 |
1980年 | 6篇 |
1979年 | 9篇 |
1978年 | 4篇 |
1976年 | 4篇 |
1975年 | 8篇 |
1969年 | 2篇 |
1968年 | 4篇 |
1967年 | 2篇 |
排序方式: 共有943条查询结果,搜索用时 11 毫秒
1.
Yoshihiro Wakayama Masahiko Inoue Makoto Murahashi Seiji Shibuya Takahiro Jimi Hiroko Kojima Hiroaki Oniki 《Annals of neurology》1996,39(2):217-223
The ultrastructural localization of adhalin and its relations to dystrophin, β-dystroglycan, and β-spectrin were studied in normal murine skeletal myofibers. The C-terminal peptides of adhalin and β-dystroglycan were synthesized based on their cDNAs, and the affinity-purified antibodies against these peptides were produced. Single-immunolabeling electron microscopy showed that the adhalin was located just inside the muscle plasma membrane or inside the myofiber a short distance from the plasma membrane. The adhalin signal was also noted at the sarcoplasmic side of plasmalemmd invaginations or at vesicular structures in subsarcolemmal areas. Double-immunogold-labeling electron microscopy disclosed a similar localization of dystrophin, β-dystroglycan, and β-spectrin. The close association of adhalin with dystrophin or β-dystroglycan was demonstrated by formation of doublets by signals of antibodies of adhalin with those of dystrophin or β-dystroglycan and was confirmed by statistical analyses. This study demonstrated that the location of adhalin is close to that of dystrophin and β-dystroglycan at the muscle plasma membrane. 相似文献
2.
3.
Dual-probe assay for rapid detection of drug-resistant Mycobacterium tuberculosis by real-time PCR 总被引:3,自引:0,他引:3 下载免费PDF全文
Wada T Maeda S Tamaru A Imai S Hase A Kobayashi K 《Journal of clinical microbiology》2004,42(11):5277-5285
Mutations in particular nucleotides of genes coding for drug targets or drug-converting enzymes lead to drug resistance in Mycobacterium tuberculosis. For rapid detection of drug-resistant M. tuberculosis in clinical specimens, a simple and applicable method is needed. Eight TaqMan minor groove binder (MGB) probes, which discriminate one-base mismatches, were designed (dual-probe assay with four reaction tubes). The target of six MGB probes was the rpoB gene, which is involved in rifampin resistance; five probes were designed to detect for mutation sites within an 81-bp hot spot of the rpoB gene, and one probe was designed as a tuberculosis (TB) control outside the rpoB gene hot-spot. We also designed probes to examine codon 315 of katG and codon 306 of embB for mutations associated with resistance to isoniazid and ethambutol, respectively. Our system was M. tuberculosis complex specific, because neither nontuberculous mycobacteria nor bacteria other than mycobacteria reacted with the system. Detection limits in direct and preamplified analyses were 250 and 10 fg of genomic DNA, respectively. The system could detect mutations of the rpoB, katG, and embB genes in DNAs extracted from 45 laboratory strains and from sputum samples of 27 patients with pulmonary TB. This system was much faster (3 h from DNA preparation) than conventional drug susceptibility testing (3 weeks). Results from the dual-MGB-probe assay were consistent with DNA sequencing. Because the dual-probe assay system is simple, rapid, and accurate, it can be applied to detect drug-resistant M. tuberculosis in clinical laboratories. 相似文献
4.
Tadaaki Yokota Yoshimi Yamashita Yoshiko Okuzono Mutsuo Takahashi Shigeyoshi Fujihara Shin'ichiro Akizuki Tokuhiro Ishihara Fumiya Uchino Takako Iwata 《Pathology international》1984,34(3):663-668
A case of malignant cystosarcoma phyllodes of the prostate is reported in a 45-year-old male. This tumor was composed of benign columnar or squamous cystic folds and sarcomatous stroma including rhabdomyomatous elements. The prostatic origin of the tumor was clearly proved by the unlabeled immunoperoxidase method. ACTA PATHOL. JPN. 34: 663–668, 1984. 相似文献
5.
Tadashi Narita Tokio Hagiwara Hiroshi Hamana Takehiko Miyasaka Akira Wakayama Tetsuo Hotta 《Macromolecular chemistry and physics.》1987,188(2):273-279
The anionic polymerizations of 2,2,2-trifluoroethyl acrylate ( 1a ), 2,2,2-trifluoroethyl methacrylate ( 1b ), 2,2,2-trifluoro-1-trifluoromethylethyl acrylate ( 2a ), and 2,2,2-trifluoro-1-trifluoromethylethyl methacrylate ( 2b ) were examined with triethylaluminium and diethylaluminium active methylene chelate compounds as initiators. 2b was polymerized with triethylaluminium. Diethyl(ethyl cyanoacetato)aluminium was found to produce polymers of 1a and 1b . Diethyl(acetylacetonato)aluminium and diethyl (dimethyl malonato)aluminium, however, show low reactivity towards these four monomers. The resulting polymers show unimodal molecular weight distribution with number-average molecular weights of 104?105, measured by GPC. The initiation reaction and the copolymerization reactivity with styrene support the anionic polymerization mechanism. 相似文献
6.
Seiji Shibuya Yoshihiro Wakayama Masahiko Inoue Makoto Murahasi Hiroaki Oniki 《Medical Electron Microscopy》1998,31(3):135-141
To examine the influence of thyroid hormone on the skeletal muscle plasma membrane, we analyzed the changes in ultrastructural architecture and membrane area complexed with digitonin of muscle plasma membrane in myopathic patients with hypo-and hyperthyroidism by the conventional freeze-fracture (F-F) technique and F-F cytochemistry using the sterol-specific ligand digitonin. The densities of flask-shaped invaginations, which are mainly thought to correspond to caveolae, intramembranous particles, and orthogonal arrays, and the changes of digitoninreacted membrane areas in the muscle plasma membranes in three patients with hypothyroid myopathy and one patient with both myasthenia gravis and hyperthyroidism were compared with those in age-matched controls. In the conventional F-F study, the muscle plasma membrane of hypothyroid patients showed increased invagination density, whereas that of the hyperthyroid patient was normal ultrastructurally. In the F-F cytochemistry study, however, the ratio of digitonin-reacted membrane areas versus fractured membrane areas was not different between hypothyroid patients and controls, whereas that of the hyperthyroid patient was lowered in comparison with that of control. These results suggest that thyroid hormone may alter the biochemical properties and ultrastructural architecture of muscle plasma membrane.This study was presented at the 29th annual meeting of the Clinical Electron Microscopy Society of Japan, Sapporo, October 2–3, 1997 相似文献
7.
8.
9.
The Epstein–Barr virus (EBV) nuclear antigen EBNA1 plays an essential role in the replication of EBV episomes in latently infected cells and is the only viral protein that is consistently expressed in all programs of latent EBV gene expression. In this study, four monoclonal antibodies (MoAbs) directed to a region (amino acid residues 442–530) of EBNA1 were generated. Competitive enzyme-linked immunosorbent assay (ELISA) experiments using biotinylated MoAbs showed that they recognized distinct epitopes. Reactivity of these MoAbs with various laboratory EBV strains and field EBV isolates was shown to be heterogeneous in that EBNA1 from certain strains (isolates) was recognized and that from others was not. All four MoAbs showed such heterogeneous reactivity, and moreover, each MoAb showed a distinct spectrum of reactivity with these EBV strains (isolates). These results demonstrate an extensive structural variation in this region of EBNA1 as predicted by previous sequencing studies. These MoAbs will be useful as probes to dissect this structural heterogeneity of EBNA1. 相似文献
10.
Hitoshi Nishimura Masashi Emoto Kenji Hiromatsu Shunsuke Yamamoto Keiko Matsuura Hiroshi Gomi Toshio Ikeda Shigeyoshi Itohara Yasunobu Yoshikai 《European journal of immunology》1995,25(5):1465-1468
The secretion of tumor necrosis factor (TNF)-α from macrophages is regulated by both priming and triggering signals. We found that macrophages from mice lacking γδ T cells [T cell receptor (TCR) δ?/- mice], which lack the gene encoding the δ chain, produced only small amounts of TNF-α in response to lipopolysaccharide (LPS) and showed a reduced level of expression of CD14. Pre-incubation of macrophages from TCR δ-/- mice with γδ T cells from their TCR δ+/- littermates restored their capacity to produce TNF-α in response to LPS. The priming activity of γδ T cells was in part inhibited by neutralizing anti-interferon (IFN)-γ monoclonal antibodies. Collectively, these results suggest that γδ T cells play a role in priming macrophages to a steady state of activation via IFN-γ secretion, which allows them to produce TNF-α when exposed to LPS. 相似文献