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Aristides D. Tagalakis Do Hyang D. Lee Alison S. Bienemann Haiyan Zhou Mustafa M. Munye Luisa Saraiva David McCarthy Zixiu Du Conrad A. Vink Ruhina Maeshima Edward A. White Kenth Gustafsson Stephen L. Hart 《Biomaterials》2014
Formulations of cationic liposomes and polymers readily self-assemble by electrostatic interactions with siRNA to form cationic nanoparticles which achieve efficient transfection and silencing in vitro. However, the utility of cationic formulations in vivo is limited due to rapid clearance from the circulation, due to their association with serum proteins, as well as systemic and cellular toxicity. These problems may be overcome with anionic formulations but they provide challenges of self-assembly and transfection efficiency. We have developed anionic, siRNA nanocomplexes utilizing anionic PEGylated liposomes and cationic targeting peptides that overcome these problems. Biophysical measurements indicated that at optimal ratios of components, anionic PEGylated nanocomplexes formed spherical particles and that, unlike cationic nanocomplexes, were resistant to aggregation in the presence of serum, and achieved significant gene silencing although their non-PEGylated anionic counterparts were less efficient. We have evaluated the utility of anionic nanoparticles for the treatment of neuronal diseases by administration to rat brains of siRNA to BACE1, a key enzyme involved in the formation of amyloid plaques. Silencing of BACE1 was achieved in vivo following a single injection of anionic nanoparticles by convection enhanced delivery and specificity of RNA interference verified by 5′ RACE-PCR and Western blot analysis of protein. 相似文献
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Demeke Mesfin Belay Wubet Alebachew Bayih Abebaw Yeshambel Alemu Aklilu Endalamaw Sinshaw Demewoz Kefale Mekonen Amare Simegn Ayele Tigabu Munye Aytenew Yeshambaw Eshetie Aynew Wasihun Hailemichael Sisay Getu Mulugeta Kiros Henok Andualem Binyam Minuye Birihan 《Tropical medicine & international health : TM & IH》2021,26(12):1539-1552
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Munye Tenaw Gashu Gezahegn 《Proceedings of the National Academy of Sciences, India. Section B.》2023,93(1):45-57
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences - Tiru Selam forest is one of the subjugated natural forest in Northwest Ethiopia. Hence, the study was... 相似文献
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