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Restriction maps of the rDNA cistron of twelve species of mosquitoes in six genera of the subfamily Culicinae were constructed using eight 6 bp recognition restriction enzymes. Anopheles albimanus was used as an outgroup. The size of the rDNA cistron ranged from 8.5 kb in Aedes katherinensis to 12.9 kb in Ae. polynesiensis. A total of twenty-six sites were scored; eighteen were polymorphic among ingroup taxa. The proportion of polymorphic nucleotide sites (Pnuc) was 0.059 and the heterozygosity per nucleotide site (Hnuc) was 0.028. Wagner and Fitch Parsimony, Dollo Parsimony and Nei-Li distance/neighbour-joining methods were used to construct phylogenetic trees. The rDNA RFLP dataset did not provide a well-supported phylogeny among culicine taxa. The RFLP phylogenies are incongruent with the morphology character based and molecular phylogenies and derived relationships did not correspond with current taxonomic classifications. The lack of resolution was due to homoplasy arising from frequent independent loss or gain of restriction sites among unrelated taxa. 相似文献
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A A Adamian B M Shloznikov L I Muzykant M A Za?denberg B Sh Gogiia Iu D Kashin 《Khirurgiia》1990,(9):77-81
The healing of sluggishly postburn wounds after local electrostimulation (ES) with the use of current-conducting carbon tissue as the electrode was studied in 12 patients. Analysis of the clinical data which were corroborated by the results of morphological and biochemical studies confirmed the marked stimulating effect of local ES which facilitates successful taking of dermal autografts and healing of burn wounds. The obtained data are evidence that the method of ES through a current-conducting carbon tissue is promising in the complex of measures for local treatment of burn wounds. 相似文献
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V P Podzolkov N A Kiselev L R Plotnikova L M Zotova L V Petrunina I N Mitina S V Kirakosian V I Mironenko L V Za?tsev I A Iurlov 《Grudnaia i serdechno-sosudistaia khirurgiia / Ministerstvo zdravookhraneniia SSSR [i] Vsesoiuznoe nauchnoe obshchestvo khirurgov》1990,(9):15-21
The article deals with the results of complex study of the effect of pulmonary insufficiency on intracardiac hemodynamics and function of the heart in the immediate and late-term periods after radical correction of Fallot's tetralogy. Answers are also given to questions concerning the expediency of the use and functional adequacy of a xeno-pericardial monocusp in the closure mechanism of the pulmonary artery valve. The study showed that massive pulmonary regurgitation has a negative effect on the functional condition of the right ventricle in late-term postoperative periods. Convincing data were obtained which allow a graft with a monocusp of a bull's pericardium to be recommended for further clinical use to prevent insufficiency of the pulmonary artery valve after radical correction of Fallot's tetralogy. 相似文献
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V M Koshkin L M Rozofarov L A Za?tseva A V Karalkin G D Saitova 《Klinichna khirurhiia / Ministerstvo okhorony zdorov'ia Ukra?ny, Naukove tovarystvo khirurhiv Ukra?ny》1990,(7):18-20
The authors have revealed a gradual development of venous hypertension and deceleration of the rate of venous blood flow in the damaged extremities in chronic obliterative arterial diseases. Decompensation of arterial hemodynamics is accompanied by the development of pronounced venous congestion with reduced venous pressure and tension. This may cause the incompetence of the deep venous valves. 相似文献
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L M Za?tsev 《Farmakologiia i toksikologiia》1988,51(3):106-110
The results of a comparative evaluation of methodical approaches to the analysis of the electrical and contractile activity of the uterus in laboratory animals during in vitro, in situ and in vivo investigation of potential uterotropic pharmacological agents are presented. For the analysis of the results there is proposed the method of mathematical processing of hysterograms including 20 parameters and the program of the calculation of the latter for the "Electronika MK-54" microcalculator. 相似文献