持续性颅内压监测在颅脑外伤治疗的效果探讨

目的对急性重型颅脑外伤患者在临床治疗中实施持续性颅内压监测的价值进行研究。方法选择本院在2017年8月—2018年8月期间收治的确诊为重型颅脑外伤的患者共计28例作为研究对象,按照硬币法将这些患者划分为两个小组,对照组14例,实验组14例。采用常规治疗方式对对照组加以治疗,采用持续性颅内压监测对实验组加以治疗,对两组患者的治疗效果进行比较。结果对照组的预后神经功能NHISS评分为(15.83±0.31)分、预后GOS评分为(3.19±0.15)分,实验组的预后神经功能NHISS评分为(11.18±0.45)分、预后GOS评分为(4.90±0.17)分。实验组预后神经功能NHISS评分、GOS评分均明显优于对照组,且差异具有统计学意义(P<0.05)。结论在急性重型颅脑外伤患者的临床治疗中应用持续性颅内压监测具有重要作用,其能够对患者的治疗予以有效指导,提升患者的治疗效果。     

胃癌D2根治术后大出血的临床分析

目的总结分析胃癌D2根治术后发生大出血的原因及治疗方法并探讨其对生存预后的影响。方法回顾性分析广东省中医院2012年1月至2016年3月258例行胃癌D2根治术患者的临床资料,根据术后是否发生大出血分为出血组和非出血组。结果14例患者(5.4%)术后发生大出血;吻合口出血、十二指肠残端瘘或破裂是出血的主要原因;二次手术和胃镜止血是主要治疗措施。两组的短期总生存期有统计学意义(1年:P=0.017,3年:P=0.011)。结论吻合口出血、十二指肠残端瘘或破裂是胃癌D2根治术后出血的主要原因,及时诊断和治疗能有效降低病死率。胃癌D2根治术后大出血会降低患者的短期总生存期。     

苍耳子药材的HPLC特征图谱研究

摘 要 目的：建立苍耳子药材的HPLC特征图谱,为评价不同产地苍耳子的质量提供参考。方法: 采用Alltima C18（250 mm×4.6 mm,5 μm）,流动相乙腈 0.1%磷酸溶液梯度洗脱,流速：0.8 ml·min^-1,检测波长：278 nm,柱温：25 ℃,对来自河南、吉林、安徽等地共47批苍耳子样品运用“中药指纹图谱相似度评价系统”进行分析。 结果:以绿原酸为参照峰,初步构建了由5个特征峰组成的苍耳子HPLC 特征图谱。结论:该方法简便、可靠,为苍耳子药材的质量控制提供了有效的评价方法。     

Comparative Study of Metal Accumulation in Three Fish Species (<Emphasis Type="Italic">Silurus asotus,Cyprinus carpio</Emphasis>, and <Emphasis Type="Italic">Carassius auratus auratus</Emphasis>) from the Jinsha and Tuo Rivers Located Upstream of the Yangtze River,China

Poland is one of the countries distinguished by a long and colorful past. Undergoing numerous turbulent socio-economic changes forced by the course of history, Poland is now one of the member states of the European Union. Experiencing low water quantity and high contamination levels in surface waters, Poland is following other EU countries in the effort to reach a “good” water status. Herein are presented impacts of changes in Polish history on water legislation, management, and research, as well as explanations for the perceptible split between engineering and scientific approaches to the aquatic issues. Drawbacks caused by unsatisfactory state research funding for the sciences and division of the water related contemporary scientific interests are also discussed.     

G-CSF-Mobilized Blood and Bone Marrow Grafts as the Source of Stem Cells for HLA-Identical Sibling Transplantation in Patients with Thalassemia Major

As an inherited anemia, thalassemia major (TM) is currently only curable with allogeneic hematopoietic stem cell transplantation (allo-HSCT). Here we report an allo-HSCT protocol for patients with TM who received a combination of granulocyte colony-stimulating factor-primed bone marrow and peripheral blood stem cells (G-BM & PBSCs) from a matched sibling donor (MSD). The conditioning regimen consisted of i.v. busulfan, cyclophosphamide, fludarabine, and antithymocyte globulin. Chimerism analysis was performed for all patients. Immunosuppressive treatment was terminated if rejection was suspected, and donor lymphocyte infusion was administered once no response was observed. A total of 184 patients with TM were enrolled in the study between July 2007 and July 2018. The cumulative incidence of grade II-IV acute graft-versus-host disease (GVHD) was 13.1%, and that of moderate or severe chronic GVHD was 5.7%. The cumulative incidence of graft rejection was .6%. In the total cohort, the 3-year overall survival, thalassemia-free survival, and GVHD-free, relapse-free survival were 97.8%, 97.3%, and 89.5%, respectively. Collectively, our results indicate that G-BM & PBSCs from an MSD is be a good stem cell source for patients with TM undergoing allo-HSCT.     

Chloride channel 3 (CIC-3) predicts the tumor size in hepatocarcinoma

Chloride channel 3 (CIC-3) has been suggested to be implicated in the carcinogenesis though; it still remains ill understood in hepatocarcinoma, especially in terms of clinicopathological meaning of its expression. Given this, herein, to understand the clinicopathological significance of CIC-3 expression in hepatocarcinoma, Immunohistochemistry was performed to examine the level of CIC-3, followed by statistical analysis of the correlation between expression versus clinicopathological variables, including gender, age, TNM classifications, tumor size, lymph node metastasis and overall prognosis. It was shown that positive staining of CIC-3 can be present in both hepatocarcinoma and its paired normal controls; and that CIC-3 was significantly over-expressed in hepatcarcioma on the whole relative to paired normal controls. Moreover, up-regulation of CIC-3 markedly correlated with tumor size and overall prognosis, suggesting that CIC-3 expression could predict both tumor size and overall prognosis in hepatocarcinoma.     

Spatial and seasonal bacterioplankton community dynamics in the main channel of the Middle Route of South-to-North Water Diversion Project

In this study, the spatial and seasonal bacterioplankton community dynamics were investigated in the main channel of the Middle Route of the South-to-North Water Diversion Project (MRP) using Illumina HiSeq sequencing. Water samples were collected in spring and summer from south to north at eight water quality monitoring stations, respectively. The results showed that seasonal changes had a more pronounced effect on the bacterioplankton community compositions (BCCs) than spatial variation. The diversity analysis results indicated that samples of summer have more operational taxonomic units (OTUs), higher richness and diversity than those in spring. The main phyla, Proteobacteria, Actinobacteria, Bacteroidetes, Cyanobacteria and Chloroflexi, displayed significant differences (P < 0.05) between spring and summer in the main channel. The Redundancy analysis (RDA) targeting all samples indicated that specific conductivity (SPC), dissolved oxygen (DO), pH and temperature (T) might be key factors in driving BCCs, while trophic status showed no significant correlation (P > 0.05). The present study provides important insights into the potential ecological roles of specific taxa in the new artificial ecosystem and it offers reference for studies on ecosystem succession of other giant interbasin water diversion project in the world.