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88例前列腺癌组织应用免疫组化、图像分析及流式细胞仪(FCM)方法,检测癌基因P~(53),c-erbB-2及增殖细胞核抗原PCNA的表达及DNA含量。P~(53)异常表达率为28%(25/88),c-erbB-2过度表达率为68%(60/88).PCNA(>50%)增殖指数值为74%(65/88).与对照组前列腺良性增生及正常前列腺组织中呈阴性反应对比有明显差异(P<0.05),与癌组织分化程度、临床分期及DNA含量无明显相关(P>0.05)。77例癌旁前列腺基底细胞异型增生上皮中有9例(12%)亦显示P~(53),c-erbB-2及PCNA的异常表达,提示该异型增生的基底细胞具有恶性表型。表明前列腺癌中P~(53),c-erbB-2及PCNA的异常表达与癌发生有一定关系,以c-erbB-2过度表达尤为重要,3种抗体的联合应用可作为前列腺癌的肿瘤标志物,对前列腺癌发病机理研究及预测早癌发生有一定价值,但作为患者预后判断的指标尚显不足。 相似文献
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Xle Ya-Gang; Han Fei-Yu; Peyrard Myrlam; Ruttledge Martin H.; Fransson Ingegerd; DeJong Pleter; Collins John; Dunham Ian; Nordenakjold Magnus; P.Dumanski Jan 《Human molecular genetics》1993,2(9):1361-1368
In order to permit detailed characterization of meningioma casesshowing deletions within chromosomal band 22q12 and furthersystematically clone genes located within this region, we establisheda genomic YAC and cosmid contig which encompasses a region inexcess of 1000 kb of 22q12. The YAC contig consists of 6 YACclones arranged Into 5 overlapping steps covering more than1100 kb. Two corresponding cosmid contigs consisting of 40 stepsof overlapping groups of cosmids encompasses 9001000kb. This set of genomic clones provides a detailed physicalmap of this part of chromosome 22 and constitutes a basis forthe Isolation and characterization of genes that may be locatedwithin this chromosomal region. Employing the exon-amplificationmethod on two cosmids from the contig, we cloned a novel, anonymousgene, pK1.3, which potentially encodes a protein of 683 aminoacids with a predicted molecular weight of of 78.5 kD. Its 2.7kb mRNA is expressed ubiquitously. We estimated the genomicsize of this gene to 100 150 kb, and it is located inthe Immediate centromeric vicinity of the neurofibromatosis2 (NF2) tumor suppressor gene. 相似文献
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