全文获取类型
收费全文 | 727篇 |
免费 | 45篇 |
国内免费 | 5篇 |
专业分类
耳鼻咽喉 | 2篇 |
儿科学 | 12篇 |
妇产科学 | 2篇 |
基础医学 | 87篇 |
口腔科学 | 23篇 |
临床医学 | 47篇 |
内科学 | 154篇 |
皮肤病学 | 1篇 |
神经病学 | 151篇 |
特种医学 | 102篇 |
外科学 | 89篇 |
综合类 | 19篇 |
预防医学 | 21篇 |
眼科学 | 15篇 |
药学 | 21篇 |
肿瘤学 | 31篇 |
出版年
2021年 | 5篇 |
2020年 | 9篇 |
2019年 | 7篇 |
2018年 | 15篇 |
2017年 | 12篇 |
2016年 | 9篇 |
2015年 | 19篇 |
2014年 | 16篇 |
2013年 | 32篇 |
2012年 | 37篇 |
2011年 | 30篇 |
2010年 | 29篇 |
2009年 | 27篇 |
2008年 | 34篇 |
2007年 | 22篇 |
2006年 | 21篇 |
2005年 | 17篇 |
2004年 | 24篇 |
2003年 | 19篇 |
2002年 | 21篇 |
2001年 | 17篇 |
2000年 | 20篇 |
1999年 | 13篇 |
1998年 | 27篇 |
1997年 | 21篇 |
1996年 | 19篇 |
1995年 | 15篇 |
1994年 | 16篇 |
1993年 | 10篇 |
1992年 | 5篇 |
1991年 | 10篇 |
1990年 | 13篇 |
1989年 | 18篇 |
1988年 | 11篇 |
1987年 | 12篇 |
1986年 | 14篇 |
1985年 | 7篇 |
1984年 | 7篇 |
1982年 | 5篇 |
1981年 | 6篇 |
1980年 | 9篇 |
1978年 | 6篇 |
1977年 | 7篇 |
1976年 | 4篇 |
1975年 | 6篇 |
1965年 | 3篇 |
1954年 | 3篇 |
1937年 | 4篇 |
1933年 | 3篇 |
1870年 | 3篇 |
排序方式: 共有777条查询结果,搜索用时 375 毫秒
1.
In the treatment of massive hematuria after renal biopsy, selective percutaneous vaso-occlusion with detachable balloons is an effective alternative to urological surgery, as demonstrated in the case of a 39-year-old man. A review of the literature, which records success rate of more than 90% in nonmalignant renal hemorrhage, confirms the therapeutic value of the various parenchyma-sparing embolization techniques. 相似文献
2.
3.
4.
H Volkmann H Kühnert M Heinke G Kahlh?fer F Meier 《Zeitschrift für die gesamte innere Medizin und ihre Grenzgebiete》1987,42(3):70-76
In 6 patients with medicamentously therapy-refractory tachycardias (3 times auricular flutter/auricular fibrillation with very rapid atrioventricular conduction, once focal atrial tachycardia, once paroxysmal atrioventricular reentry tachycardia, once recurrent ventricular tachycardia on the basis of an interventricular reentry) percutaneous transvenous catheter ablations of the atrioventricular conduction region (bundle of His ablation, 5 cases) and the right Tawara branch (1 case), respectively, were performed. During a follow-up period of 2-26 months (on an average 10.5 +/- 8.0 months) a permanent total block in 4 cases could be obtained (3 times AV-block III. once complete right bundle branch block). In two other patients the total AV block receded to the AV block I. and II. respectively, within 2 and 8 days, respectively; in the first case the AV node reentry tachycardias were no more to be evoked, in the second case a tolerable ventricular frequency was the result during the auricular flutter recidivations. In all patients treated by means of catheter ablation after the intervention an impressive improvement of the clinical symptomatology developed. Due to possible complication and the dependence upon the pacemaker of the patients concerned which is to be expected the indication to the ablation should be made only after the exhaustion of all medicamentous possibilities and taking into consideration other electric therapy methods. 相似文献
5.
A Klumbies R Paliege H Volkmann 《Zeitschrift für die gesamte innere Medizin und ihre Grenzgebiete》1988,43(13):348-352
Repetitive precordial thumping is the simplest method of temporary cardiac pacing. In 90 patients out of 100 with witnessed cardiac arrest because of asystole or marked bradycardia the critical situation could be effectively bridged over by this until a sufficient spontaneous rhythm returned or electrostimulation was ready to function. 69 patients were conscious during the stimulation. During the mechanical pacing only in 2 patients there occurred ventricular flutter or fibrillation, which was stopped by electric defibrillation. The existence of the myocardial contractility is the presupposition for effective mechanical and electrical pacing. 相似文献
6.
Martin Krause Wolfgang Fogel Volker Tronnier Sabine Pohle Konstanze H?rtnagel Ute Thyen Jens Volkmann 《Movement disorders》2006,21(12):2255-2257
Pantothenate kinase-associated neurodegeneration (PKAN) is a rare autosomal recessive disorder with onset in childhood and rapid progression. There is no causative and insufficient symptomatic drug therapy. Deep brain stimulation (DBS) of the internal pallidum (GPi) has been reported to improve motor function. Most case reports, however, are limited to short observational periods. The impact of DBS on the progression and life expectancy in PKAN is unknown. We present a 5-year outcome and video documentation of bilateral GPi-DBS of an adolescent patient suffering from genetically defined PKAN. 相似文献
7.
We performed high-resolution magnetic resonance morphometry of the total midsagittal area and seven midsagittal subareas of the corpus callosum in healthy young adult dextrals and sinistrals (N = 52). There was no influence of handedness on these anatomic measurements. However, an effect of sex emerged, with women (N = 26) having a larger proportional isthmus segment of the callosum. This may reflect a sex-specific difference in the interhemispheric connectivity and functional organization of the temporoparietal association cortex. 相似文献
8.
9.
Androgen receptor YAC transgenic mice carrying CAG 45 alleles show trinucleotide repeat instability 总被引:1,自引:15,他引:1
La Spada AR; Peterson KR; Meadows SA; McClain ME; Jeng G; Chmelar RS; Haugen HA; Chen K; Singer MJ; Moore D; Trask BJ; Fischbeck KH; Clegg CH; McKnight GS 《Human molecular genetics》1998,7(6):959-967
X-linked spinal and bulbar muscular atrophy (SBMA) is caused by a CAG
repeat expansion in the first exon of the androgen receptor (AR) gene.
Disease-associated alleles (37-66 CAGs) change in length when transmitted
from parents to offspring, with a significantly greater tendency to shift
size when inherited paternally. As transgenic mice carrying human AR cDNAs
with 45 and 66 CAG repeats do not display repeat instability, we attempted
to model trinucleotide repeat instability by generating transgenic mice
with yeast artificial chromosomes (YACs) carrying AR CAG repeat expansions
in their genomic context. Studies of independent lines of AR YAC transgenic
mice with CAG 45 alleles reveal intergenerational instability at an overall
rate of approximately 10%. We also find that the 45 CAG repeat tracts are
significantly more unstable with maternal transmission and as the
transmitting mother ages. Of all the CAG/CTG repeat transgenic mice
produced to date the AR YAC CAG 45 mice are unstable with the smallest
trinucleotide repeat mutations, suggesting that the length threshold for
repeat instability in the mouse may be lowered by including the appropriate
flanking human DNA sequences. By sequence-tagged site content analysis and
long range mapping we determined that one unstable transgenic line has
integrated an approximately 70 kb segment of the AR locus due to
fragmentation of the AR YAC. Identification of the cis - acting elements
that permit CAG tract instability and the trans -acting factors that
modulate repeat instability in the AR YAC CAG 45 mice may provide insights
into the molecular basis of trinucleotide repeat instability in humans.
相似文献
10.
Use of specific ELISA for the detection of antibodies directed against p53 protein in patients with hepatocellular carcinoma. 总被引:3,自引:0,他引:3
下载免费PDF全文
![点击此处可从《Journal of clinical pathology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
S D Ryder P M Rizzi M Volkmann E Metivier L M Pereira P R Galle N V Naoumov H Zentgraf R Williams 《Journal of clinical pathology》1996,49(4):295-299
AIMS: To analyse the significance of antibodies to p53 protein as a serological marker for changes in p53 gene expression in patients with hepatocellular carcinoma. METHODS: Thirty eight patients with hepatocellular carcinoma, 19 showing accumulation of p53 protein by immunohistochemistry and 19 having no accumulation, were studied. The presence of anti-p53 was tested using a novel ELISA utilising a recombinant p53 protein as a capture system and verified by western blotting. p53 gene mutations were sought by single strand conformational polymorphism and DNA sequencing analyses. RESULTS: Of 19 patients with p53 protein accumulation in tumour tissue, 10 (52%) had antibodies to p53 in serum by ELISA. Four patients with p53 negative immunohistochemistry also had detectable anti-p53. Western blot analysis confirmed the specificity of the ELISA positive serum samples. The presence of anti-p53 was independent of serum alpha-fetoprotein and was detected in 50% of small tumours while only 8% were alpha-fetoprotein positive. Mutations affecting exons 5 and 6 seem to be more frequently associated with development of anti-p53, than mutations in exons 7 or 8. CONCLUSIONS: The ELISA for anti-p53 is a convenient and specific tet for the detection of humoral response to alterations in p53 gene expression and could be of value in the diagnosis and characterisation of patients with hepatocellular carcinoma. 相似文献