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1.
When stimulating the mixed nerve to record evoked potential, both sensory and motor fibers are activated before entering the spinal cord. The N10 potential has been described as an antidromic motor evoked potential based on results obtained by recording at the anterior midneck. In the present study, we examined the changes in latencies of Erb's potential, N10, and N13 by stimulating the median nerve distally at the wrist and proximally at the elbow. The conduction velocity of N10 calculated by the difference between N10 latencies at the two stimulation points was consistent with motor conduction velocity, although N13 conduction velocity estimated by the same method reflected a sensory conduction velocity. A positive relation was also observed between the indirect latency from the stimulation point to the anterior root as calculated using the equation (F - M - 1) / 2 (ms) and the direct latency to the negative peak of the N10 potential. Our data support the notion that N10 represents antidromic motor potential originating in the spinal entry zone of the anterior root. 相似文献
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The transmembrane form of TNF-alpha drives autoantibody production in the absence of CD154: studies using MRL/Mp-Fas(lpr) mice 总被引:1,自引:0,他引:1
It is generally accepted that the interaction between CD40 and its ligand (CD154) plays a decisive role in contact-dependent help for T and B cells. In CD154-deficient MRL/Mp-Fas(lpr) (MRL/lpr) mice, however, high titres of IgG2a-type autoantibodies against small nuclear ribonucleoproteins (snRNPs) are observed. We successfully isolated two CD154-deficient MRL/lpr Th1 lines, which could provide B cell help for anti-snRNP antibody production. The proliferative responses of the Th1 cell lines were MHC class II (I-Ek)-restricted. Although syngeneic B cell proliferation was induced by Th1 lines in both a contact-dependent and -independent manner, the soluble form of TNF-alpha (sTNF-alpha) was not involved in contact-independent B cell proliferation. On the other hand, both anti-TNF-alpha and TNF-receptor 2 (TNF-R2, p75) monoclonal antibody (MoAb) blocked contact-dependent B cell proliferation, suggesting that the transmembrane form of TNF-alpha (mTNF-alpha)-TNF-R2 co-stimulation participates in B cell activation. Similarly, anti-TNF-alpha and TNF-R2 MoAb inhibited anti-snRNP antibody production in vitro, but anti-CD154 or TNF-R1 MoAb did not. These results indicate that the interaction of mTNF-alpha on activated Th1 cells with TNF-R2 on B cells may be involved in the autoimmunity seen in MRL mice, and that the blockade of CD40-CD154 co-stimulation may not always be able to suppress some Th1-related manifestations of lupus. 相似文献
5.
Li J Mori M Yang Y Inoue H Mimori K Shibuta K Nakashima H Mafune K Shimada Y Barnard G Sugimachi K Akiyoshi T 《International journal of oncology》1995,7(2):257-260
The multiple tumor suppressor 1 (MTS1) gene is homozygously deleted frequently in cell lines derived from a wide variety of tumors. We investigated the deletion of the MTS1 gene in esophageal cancer cell lines and primary esophageal squamous carcinomas using the polymerase chain reaction. Sixteen and 15 of 23 esophageal cancer cell lines showed homozygous deletion of MTS1 exon 1 and exon 2, respectively, while none of 21 primary esophageal carcinomas showed the deletion. An analysis of MTS1 gene mutations was carried out by direct DNA sequencing in 8 cell lines and 21 primary carcinomas showing no homozygous deletion. In contrast to previous reports of esophageal carcinoma, there were no mutations recognized in the region sequenced. Our study suggests that the inactivation of the MTS 1 gene may play an important role in esophageal carcinoma cell lines but may be less important in primary carcinomas of the human esophagus. 相似文献
6.
Serial diffusion-weighted imaging in MELAS 总被引:7,自引:1,他引:6
Ohshita T Oka M Imon Y Watanabe C Katayama S Yamaguchi S Kajima T Mimori Y Nakamura S 《Neuroradiology》2000,42(9):651-656
Clinical features of mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes (MELAS) resemble those
of cerebral infarcts, but the pathogenesis of infarct-like lesions is not fully understood. To characterise these infarct-like
lesions, we studied two patients with MELAS using diffusion-weighted (DWI) MRI before and after stroke-like episodes and measured
the apparent diffusion coefficient (ADC) in the new infarct-like lesions. These gave high signal on DWI and had much higher
ADC than normal-appearing regions. The ADC remained high even 30 days after a stroke-like episode then decreased in lesions,
with or without abnormality as shown by conventional MRI. We speculate that early elevation of ADC in the acute or subacute
phase reflects vasogenic rather than cytotoxic edema. The ADC of the lesions, which disappeared almost completely with clinical
improvement, returned to normal levels, which may reflect tissue recovery without severe damage. To our knowledge, this is
the first study of DWI in MELAS.
Received: 13 September 1999/Accepted: 7 January 2000 相似文献
7.
Hisashi Nagahara Koshi Mimori Mitsuhiko Ohta Tohru Utsunomiya Hiroshi Inoue Graham F Barnard Masaichi Ohira Kosei Hirakawa Masaki Mori 《Clinical cancer research》2005,11(4):1368-1371
PURPOSE: Somatic mutations of the epidermal growth factor receptor (EGFR) gene may predict the sensitivity of non-small cell lung carcinoma to gefitinib. However, no mutations have been reported for colorectal carcinoma. We therefore analyzed EGFR mutations in colorectal adenocarcinomas by the combined use of laser microdissection and sequencing of genomic DNA. EXPERIMENTAL DESIGN: We examined 11 representative colorectal adenocarcinoma cell lines and 33 clinical samples of colorectal carcinoma. In the clinical cases, we carefully dissected only carcinoma cells from frozen sections by laser microdissection. After DNA extraction and PCR, we examined EGFR mutations by sequencing genomic DNA. RESULTS: None of 11 colorectal carcinoma cell lines exhibited somatic mutations, but 4 of 33 clinical tumors (12%) exhibited mutations in the EGFR kinase domain. This may be the first report of somatic mutations in colorectal adenocarcinoma. CONCLUSIONS: Our findings suggest that a distinct minority of colorectal adenocarcinomas exhibit somatic mutations of EGFR, and these tumors may be susceptible to gefitinib treatment. 相似文献
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9.
A. Sasaki F. Tanaka K. Mimori H. Inoue S. Kai K. Shibata M. Ohta S. Kitano M. Mori 《European journal of surgical oncology》2008
Aims
CD4+ CD25+ forkhead box P3 (FOXP3)+ Treg accumulate in malignant tumors and negatively regulate anti-tumor immunity. To determine the prognostic value of tumor-infiltrating regulatory T cells (Treg), we conducted a retrospective study on 164 patients with hepatocellular carcinoma (HCC) who underwent curative hepatic resection.Methods
We investigated the number of tumor-infiltrating FOXP3+ Treg in formalin-fixed HCC specimens. The number of FOXP3+ Treg for each case was calculated as the total number of positive cells per 10 high-power fields (HPF) on light microscopy. Long-term survival rate after resection according to the number of FOXP3+ Treg was accessed by univariate and multivariate analyses.Results
The mean and median numbers of tumor-infiltrating Treg were 29.0 and 14 per 10 HPF for FOXP3+ Treg. The number of FOXP3+ Treg was positively correlated with preoperative serum alpha-fetoprotein levels. The disease-free survival rate was significantly lower in patients with high Treg counts (≥14, n = 84) than in those with low Treg counts (<14, n = 80) (13.6% vs. 25.7% at 5 years; P = 0.02). By multivariate analysis, the high Treg counts, presence of portal vein invasion, and elevation of preoperative aspartate aminotransferase level were independent predictive factors of tumor recurrence.Conclusions
The high number of tumor-infiltrating Treg is an independent predictive factor of tumor recurrence after hepatic resection for HCC. 相似文献10.