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1.
Polymorphisms and the differential antiviral activity of the chicken Mx gene 总被引:29,自引:0,他引:29 下载免费PDF全文
Ko JH Jin HK Asano A Takada A Ninomiya A Kida H Hokiyama H Ohara M Tsuzuki M Nishibori M Mizutani M Watanabe T 《Genome research》2002,12(4):595-601
The nucleotide sequence of chicken Mx cDNA was reported earlier using the White Leghorn breed in Germany, but it showed no enhanced resistance to viruses. In this study, the nucleotide sequences of chicken Mx cDNA were determined in many breeds. A total of 25 nucleotide substitutions, of which 14 were deduced to cause amino acid exchanges, were detected, suggesting that the chicken Mx gene is very polymorphic. Transfected cell clones expressing chicken Mx mRNA were established after the Mx cDNA was constructed with an expression vector and introduced into mouse 3T3 cells, and the Mx genes from some breeds were demonstrated to confer positive antiviral responses to influenza virus and vesicular stomatitis virus. On the basis of the comparison among the antiviral activities associated with many Mx variations, a specific amino acid substitution at position 631 (Ser to Asn) was considered to determine the antivirally positive or negative Mx gene. Thus, a single amino acid substitution influences the antiviral activity of Mx in domesticated chickens. 相似文献
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Susaki Y; Shimizu S; Katakura K; Watanabe N; Kawamoto K; Matsumoto M; Tsudzuki M; Furusaka T; Kitamura Y; Matsuda H 《Blood》1996,88(12):4630-4637
The stimulating effect of nerve growth factor (NGF) on phagocytosis, parasite killing, and interleukin-1beta (IL-1beta) production of murine peritoneal macrophages was assessed. In the presence of various doses of NGF, macrophages showed the increased phagocytosis of both nonspecific hydrophilic microspheres and sheep red blood cells (SRBC) opsonized with anti-SRBC antibodies (Ab) or complement in a dose- dependent manner. NGF also enhanced killing of Leishmania donovani promastigotes by macrophages, and its ability was comparable with that of an optimal dose of recombinant granulocyte-macrophage colony- stimulating factor or recombinant interferon-gamma. The addition of NGF to peritoneal macrophages and monocyte-macrophage J774A.1 cells led to a significant release of IL-1beta in a dose-dependent manner and expression of IL-1beta mRNA. Because pretreatment of peritoneal macrophages and J774A.1 cells with K-252a, a tyrosine kinase inhibitor, completely suppressed these NGF-mediated stimulating effects and p140trk phosphorylation and because flow cytometric analysis with specific Ab against two distinct NGF receptors showed the expression of p140trk, unlike p75LNGFR, on the surface of macrophages, the stimulating activity of NGF to murine macrophages may be mediated through p140trk. Thus, NGF may act as an activator for murine macrophages in the process of inflammatory and immune actions. 相似文献
4.
Satoshi Ishishita Yuri Tsuruta Yoshinobu Uno Atsushi Nakamura Chizuko Nishida Darren K. Griffin Masaoki Tsudzuki Tamao Ono Yoichi Matsuda 《Chromosome research》2014,22(1):15-34
Many families of centromeric repetitive DNA sequences isolated from Struthioniformes, Galliformes, Falconiformes, and Passeriformes are localized primarily to microchromosomes. However, it is unclear whether chromosome size-correlated homogenization is a common characteristic of centromeric repetitive sequences in Aves. New World and Old World quails have the typical avian karyotype comprising chromosomes of two distinct sizes, and C-positive heterochromatin is distributed in centromeric regions of most autosomes and the whole W chromosome. We isolated six types of centromeric repetitive sequences from three New World quail species (Colinus virginianus, CVI; Callipepla californica, CCA; and Callipepla squamata, CSQ; Odontophoridae) and one Old World quail species (Alectoris chukar, ACH; Phasianidae), and characterized the sequences by nucleotide sequencing, chromosome in situ hybridization, and filter hybridization. The 385-bp CVI-MspI, 591-bp CCA-BamHI, 582-bp CSQ-BamHI, and 366-bp ACH-Sau3AI fragments exhibited tandem arrays of the monomer unit, and the 224-bp CVI-HaeIII and 135-bp CCA-HaeIII fragments were composed of minisatellite-like and microsatellite-like repeats, respectively. ACH-Sau3AI was a homolog of the chicken nuclear membrane repeat sequence, whose homologs are common in Phasianidae. CVI-MspI, CCA-BamHI, and CSQ-BamHI showed high homology and were specific to the Odontophoridae. CVI-MspI was localized to microchromosomes, whereas CVI-HaeIII, CCA-BamHI, and CSQ-BamHI were mapped to almost all chromosomes. CCA-HaeIII was localized to five pairs of macrochromosomes and most microchromosomes. ACH-Sau3AI was distributed in three pairs of macrochromosomes and all microchromosomes. Centromeric repetitive sequences may be homogenized in chromosome size-correlated and -uncorrelated manners in New World quails, although there may be a mechanism that causes homogenization of centromeric repetitive sequences primarily between microchromosomes, which is commonly observed in phasianid birds. 相似文献
5.
Tsuguhiko Seo Atsushi Tatsuguchi Seiichi Shinji Masaoki Yonezawa Keigo Mitsui Shu Tanaka Shunji Fujimori Katya Gudis Yuh Fukuda Choitsu Sakamoto 《Virchows Archiv : an international journal of pathology》2009,454(6):667-676
The aim of this study is to investigate the expression of three prostaglandin E synthase (PGES) isomers in colorectal cancer
(CRC) tissue and to evaluate their relationship to clinicopathological factors and patient prognosis. Microsomal PGES (mPGES)-1,
mPGES-2, cytosolic PGES (cPGES) and cyclooxygenase (COX)-2 protein expression were analyzed by real-time polymerase chain
reaction and Western blot. The localization of each PGES and COX-2 protein was examined by immunohistochemistry in 155 surgical
resections and correlated to clinicopathological factors and patient prognosis. mPGES-1 mRNA and protein levels were significantly
higher in CRC than in paired normal tissues. mPGES-1 immunoreactivity localized in cancer cells in 43% of cases. mPGES-2 immunoreactivity
was significantly more pronounced in cancer cells than in adjacent normal epithelium in 36% of cases. cPGES immunoreactivity
was homogeneous in cancer cells and thus determined constitutive. mPGES-1 and mPGES-2 correlated with significantly worse
prognosis in stage I–III patients. These results indicate that mPGES-1 and mPGES-2 may each play a role in CRC progression. 相似文献
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Akira Yoshida Tarou Asaga Chihiro Masuzawa Satoru Kawahara Shunsuke Yanoma Masaoki Harada Takasi Okamoto 《Journal of surgical oncology》1994,55(2):104-107
Five cell lines were established from four undifferentiated carcinomas and one squamous cell carcinoma of the thyroid. The levels of several kinds of cytokines were measured in the conditioned media of these cell lines by enzyme-linked immunosorbent assay (ELISA). Interleukin-6 (IL-6) was produced by four of the five cell lines, interleukin-1α (IL-lα) by three cell lines, and granulocyte-colony stimulating factor (G-CSF) by two cell lines. The mRNA of IL-lα or IL-6 was detected by Northern blot analysis in all the cell lines which secreted these cytokines into culture medium. These results suggest that undifferentiated carcinoma and squamous cell carcinoma of the thyroid frequently produce cytokines. Further studies are needed to clarify the possible clinical effects of these cytokines in patients with thyroid carcinoma. © Wiley-Liss, Inc. 相似文献
8.
Tetsuro Konno Akira Kakita Takeyasu Taira Masaoki Minato Akira Tamaki Eisei Sasaki Yoichi Kasai 《Surgery today》1981,11(3):209-213
The possible benefit of oxygenation during initial cold flushing was investigated as a means of improving the quality of liver
preservation in rats. In five groups of animals (total 61 experiments), the livers were flushed with different perfusates.
Non-oxygenated groups included controls, Collins’ solution alone and Collins’ solution containing perfluorotributylamine (FC-43).
In the oxygenated groups, Collins’ solution alone and Collins’ solution containing FC-43 were oxygenated by bubbling. The
hepatic ATP level and histopathological changes were used to assess the quality of liver preservation. Oxygenation during
the initial cooling process proved to be effective in maintaining energy metabolism and preventing the characteristic microscopic
changes of ischemic damage. Oxygenated Collins’ solution containing FC-43 showed a much longer lasting effect compared with
oxygenated Collins’ solution alone, without FC-43. Under light microscopy, the integrity of the liver appeared to be well
preserved up to eight hours with the former solution. It is concluded that enhanced oxygenation with FC-43 in the initial
cold flushing period can improve the quality of liver preservation. 相似文献
9.
M Kamiya T Eimoto H Kishimoto T Tsudzuki H Morishita Y Wada T Wakabayashi T Hashimoto S I Goodman F E Frerman 《Pediatric pathology》1990,10(6):1007-1019
An autopsy study of glutaric aciduria type II in a 62-day-old Japanese boy is presented. The diagnosis was made by analysis of organic acids in the urine. Immunoblot analysis of liver homogenate confirmed the diagnosis, revealing absence of electron-transferring flavoprotein dehydrogenase. The major findings were fatty changes of variable degree in many organs and tissues, the most severe being found in cardiac myocytes, hepatocytes, renal tubular epithelium, and skeletal muscle fibers. Other pertinent findings included multicystic and dysplastic kidney, pulmonary alveolar proteinosis, and spongiosis and gliosis of the spinal cord. The thymus was markedly depleted, and lymphocytes in the lymph nodes were mainly B cells. Although some of these changes may have been secondary to the sepsis and immunosuppression complicating 2 months of intensive care, the abnormal organic acid metabolism with severe acidosis may have been a significant contributing factor. 相似文献
10.
Soichiro Kitamura Yasunaru Kawashima Kanji Kawachi Masaoki Fujino Takahiro Kozuka Tsuyoshi Fujita Hisao Manabe 《The American journal of cardiology》1977
Reports of a mucocutaneous lymph node syndrome that frequently affects the coronary arteries have been increasing in Japan and other countries including the United States. Twelve patients with a documented history of mucocutaneous lymph node syndrome or a related coronary arteritis were studied with right and left heart catheterization, cardiac output measurements, left cineventriculography and selective coronary arteriography. These patients were separated into two groups on the basis of coronary arteriographic findings. Group 1 comprised six patients with normal coronary arteriograms; group 2 included the remaining six patients with coronary arterial aneurysm or occlusion. 相似文献