全文获取类型
收费全文 | 5940篇 |
免费 | 360篇 |
国内免费 | 43篇 |
专业分类
耳鼻咽喉 | 60篇 |
儿科学 | 104篇 |
妇产科学 | 55篇 |
基础医学 | 1027篇 |
口腔科学 | 66篇 |
临床医学 | 488篇 |
内科学 | 1071篇 |
皮肤病学 | 119篇 |
神经病学 | 718篇 |
特种医学 | 309篇 |
外科学 | 910篇 |
综合类 | 16篇 |
一般理论 | 3篇 |
预防医学 | 147篇 |
眼科学 | 236篇 |
药学 | 379篇 |
中国医学 | 14篇 |
肿瘤学 | 621篇 |
出版年
2024年 | 4篇 |
2023年 | 51篇 |
2022年 | 93篇 |
2021年 | 142篇 |
2020年 | 109篇 |
2019年 | 134篇 |
2018年 | 143篇 |
2017年 | 113篇 |
2016年 | 177篇 |
2015年 | 218篇 |
2014年 | 243篇 |
2013年 | 312篇 |
2012年 | 468篇 |
2011年 | 471篇 |
2010年 | 290篇 |
2009年 | 268篇 |
2008年 | 408篇 |
2007年 | 430篇 |
2006年 | 399篇 |
2005年 | 422篇 |
2004年 | 380篇 |
2003年 | 334篇 |
2002年 | 281篇 |
2001年 | 63篇 |
2000年 | 42篇 |
1999年 | 32篇 |
1998年 | 49篇 |
1997年 | 25篇 |
1996年 | 31篇 |
1995年 | 21篇 |
1994年 | 25篇 |
1993年 | 20篇 |
1992年 | 9篇 |
1991年 | 13篇 |
1990年 | 9篇 |
1989年 | 11篇 |
1988年 | 3篇 |
1987年 | 4篇 |
1986年 | 6篇 |
1985年 | 7篇 |
1984年 | 6篇 |
1983年 | 14篇 |
1982年 | 12篇 |
1981年 | 6篇 |
1980年 | 3篇 |
1979年 | 5篇 |
1978年 | 7篇 |
1977年 | 4篇 |
1972年 | 6篇 |
1965年 | 4篇 |
排序方式: 共有6343条查询结果,搜索用时 15 毫秒
1.
2.
Carsten Olbrich Andrea Gessner Werner Schr?der Oliver Kayser Rainer H Müller 《Journal of controlled release》2004,96(3):425-435
Sleeping sickness is a widely distributed disease in great parts of Africa. It is caused by Trypanosoma brucei gambiense and rhodiense, transmitted by the Tse-Tse fly. After a hemolymphatic stage, the parasites enter the central nervous system where they cannot be reached by hydrophilic drugs. To potentially deliver the hydrophilic antitrypanosomal drug diminazene diaceturate to the brain of infected mice, the drug was formulated as lipid-drug conjugate (LDC) nanoparticles (NP) by combination with stearic- (SA) and oleic acid (OA). To estimate the in vivo compatibility, the particles were incubated with human granulocytes. Because as potential delivery mechanism the absorption of specific serum proteins (ApoE, Apo AI and Apo AIV) was found to be responsible for the delivery of nanoparticles to the brain, demonstrated using PBCA nanoparticles coated with polysorbate 80 (LDL uptake mechanism) the nanoparticles were incubated with mouse serum and the adsorption pattern was determined using the 2-D PAGE technique. As a result of this study, the cytotoxic potential was shown to decrease when diminazene is part of the particle matrix compared to pure fatty acid nanoparticles and the mouse serum protein adsorption pattern differs from the samples studied earlier in human serum. Especially, the fact concerning Apo-E that could be detected when the particles were incubated in human serum is absent after the mouse serum incubation, potentially, is a critical point for the delivery via the LDL-uptake mechanism but the data demonstrate that LDC nanoparticles, with 33% (wt/wt) drug loading capacity possess the potential to act as a delivery system for hydrophilic drugs like diminazene diaceturate and that further studies have to demonstrate the usability as a brain delivery system. 相似文献
3.
Streptococcus sanguis, usually considered a nonpathogen of the oral cavity, was isolated from blood cultures from a patient who was subsequently found to have a cecal adenocarcinoma. Further studies are needed to determine if Streptococcus sanguis infections have diagnostic implications similar to those of Streptococcus bovis. © 1995 Wiley-Liss, Inc. 相似文献
4.
5.
Astrid R. R. Heutelbeck Carsten Junghans Hermann Esselmann Ernst Hallier Thomas G. Schulz 《International archives of occupational and environmental health》2009,82(9):1123-1131
Introduction
Cattle are an important source of allergens in the working area of farmers. Asthma caused by cow allergens is a significant occupational problem. Yet in allergological testing, the results of in vivo and in vitro diagnostic tests are often inconsistent even in cases with clearly cattle-related symptoms. 相似文献6.
7.
The present study was designed to investigate whether or not arginine vasopressin (AVP) is released from magnocellular neurons within the median eminence (ME) in vivo. Urethane-anesthetized adult male Wistar rats were equipped with a microdialysis probe aimed at the supraoptic (SON) or paraventricular nucleus (PVN), a push-pull perfusion probe resting in the ME, and a blood microdialysis probe within the jugular vein. Dialysis of the SON (but not the PVN) with Ringer's solution containing 56 mmol l−1 K+ resulted in an increase in AVP release within the ME (to 492 ± 192% of release during basal conditions,P < 0.05) and into blood (to 138 ± 9%,P < 0.01) whereby the release probably occurred from axonal swellings and nerve terminals of supraoptic neurons which project through the internal zone of the ME to the posterior pituitary. The calculated amount of AVP released into the extracellular fluid of the ME was high enough (approximately 1 pg/μ1) to hypothesize that the neuropeptide could enter the portal blood capillaries in physiologically relevant concentrations. Taken together, the present study indicates that activation of magnocellular neurons is accompanied by release of AVP within the median eminence. We assume that AVP released in this way might mediate a communication between the hypothalamic-neurohypophysial system and the hypothalamic-pituitary-adrenal axis in response to selected stressful stimuli. 相似文献
8.
This article concerns the relations between personality and quality of life. In the first part, we discuss different conceptualizations of personality and quality of life. We argue that personality affects quality of life by influencing how people approach and react to critical life situations. In the second part, we address the beneficial role played by two individual difference variables in promoting quality of life: dispositional optimism and goal adjustment. Literature is reviewed demonstrating that dispositional optimism facilitates subjective well-being and good health, mediated by a person's coping behaviors. In addition, we discuss studies that examine people who confront unattainable goals. The reported evidence supports the conclusion that individual differences in people's abilities to adjust to unattainable goals are associated with a good quality of life. 相似文献
9.
A method is described based on crossed immunoelectrophoresis of a complex antigen mixture in agarose gel followed by incubation of the gel with the monoclonal antibody. The bound monoclonal antibody is detected by the use of a secondary enzyme-labelled antibody.Using this technique we have been able to identify the precipitate arc in crossed immunoelectrophoresis of major histocompatibility complex (MHC) class I molecules in a mixture of all detergent solubilized cell membrane molecules by means of a monoclonal antibody, the specificity of which was known independently to be against MHC class I molecules. In other experiments using the same technique we demonstrated the reaction of a monoclonal antibody specific for chicken Ig light chains. 相似文献