A program in BASIC for calculation of tissue ion flux rates, rate constants, and cellular ion contents and concentrations for a three compartmental serial model

A BASIC program is presented to calculate tissue ion flux rates, rate constants, and cellular ion contents and concentrations from ion flux curves. For the purpose of this program, the biological tissue will be treated as a three compartmental series model. The program requires that eight experimentally determined variables be entered for each ion species to be analyzed: the experimental slope and intercept variables for each phase of the flux curve, the wet and dry tissue weights, the known or estimated value of extracellular space and the measured or known value of the cellular surface to volume ratio of the particular tissue being studied. All other required input data are obtained from the literature or experimental protocol and may be treated as constants. This program was designed for the in vitro determination of ion exchange in skeletal muscle but may be used with little or no modification for flux measurements in other tissues.