全文获取类型
收费全文 | 66篇 |
免费 | 0篇 |
专业分类
儿科学 | 7篇 |
妇产科学 | 1篇 |
基础医学 | 2篇 |
口腔科学 | 2篇 |
临床医学 | 5篇 |
内科学 | 19篇 |
神经病学 | 4篇 |
外科学 | 11篇 |
预防医学 | 1篇 |
药学 | 11篇 |
肿瘤学 | 3篇 |
出版年
2013年 | 1篇 |
2012年 | 1篇 |
2010年 | 1篇 |
2007年 | 1篇 |
2006年 | 5篇 |
2004年 | 1篇 |
2003年 | 3篇 |
2002年 | 3篇 |
1999年 | 2篇 |
1998年 | 6篇 |
1997年 | 3篇 |
1996年 | 5篇 |
1995年 | 3篇 |
1994年 | 2篇 |
1993年 | 2篇 |
1992年 | 2篇 |
1991年 | 2篇 |
1990年 | 1篇 |
1988年 | 4篇 |
1987年 | 4篇 |
1986年 | 4篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1981年 | 1篇 |
1979年 | 1篇 |
1977年 | 1篇 |
1954年 | 3篇 |
排序方式: 共有66条查询结果,搜索用时 15 毫秒
1.
SHIGEKI OCHI MORIKAZU ONJI KAZUHITO SHIRAISHI KYOKO OHTU TOMOHIRO AKAO YOSHITO YANO NOBUYUKI TAKEI HIKARU MATSUI YASUYUKI OHTA MASAKICHI UMEDA 《Journal of gastroenterology and hepatology》1991,6(6):599-602
To clarify the prevalence of concurrent infection with hepatitis C virus (HCV), hepatitis B virus (HBV) and human T cell leukaemia virus (HTLV), we measured HCV antibody in the population of a district endemic for HBV and HTLV infection. Blood samples were collected in June 1990 from 579 inhabitants of four islands of Uwa Bay in the southwest of Ehime Prefecture in Japan. Anti-HCV antibody against C100-3 protein was detected using an enzyme-linked immunosorbent assay kit (Ortho Diagnostics). Thirteen of the 579 inhabitants (2.2%) were positive for anti-HCV, and this prevalence rate was not significantly different from the frequency of anti-HCV in Tokyo blood donors. A total of 11% (64 of 579) of the subjects were positive for HBsAg and 3.3% (19 of 579) were positive for anti-HTLV. These frequencies of HBsAg and anti-HTLV positivity were distinctly higher than the respective means of Japanese. All anti-HCV positive individuals were negative for HBsAg and anti-HTLV, while 54% (7 of 13) had increased alanine aminotransferase levels. These data suggest that the prevalence of HCV infection is not high even in an area endemic for HBV and HTLV infection. 相似文献
2.
SHINICHI KOBAYASHI KAORU HIGUCHI HISASHI TAMAKI YASUYUKI WADA NORIYUKI WADA MASAKATSU KUBO YUICHI KOIKE MASATO NAGATA OSAMU TATSUZAWA SATOSHI FUJIKAWA 《Pediatrics international》1997,39(2):257-262
Questionnaires were sent to 1290 hospitals in Japan asking for data on patients with juvenile dermatomyositis (JDM) diagnosed between June 1984 and May 1994. Of the 204 patients identified by these questionnaires, 102 met the criteria for JDM. JDM is categorized into three subtypes: Banker-type JDM , Brunsting-type and fulminant-type; patients with the latter exhibit markedly elevated serum levels of creatinine phosphokinase (> 10 000 U/mL) and appear to be at risk of renal failure. Cutaneous manifestations were present in 98% of patients and preceded the appearance of other symptoms. This tendency is one of the reasons for the difficulty in some cases in diagnosing the onset of JDM. Better criteria for early treatment of JDM are needed. The results of the present study suggest that itching and calcinosis are factors that indicate a poor prognosis in patients with JDM. Muscle enzyme levels do not always reflect disease activity, suggesting that methods other than measurement of muscle enzymes, such as measurement of the levels of neoprerin and von Willebrand factor antigen, as well as magnetic resonance imaging should be used to be evaluate disease severity. Patients with Brunsting-type JDM who exhibit dysphagia and antinuclear antibody positivity and patients with Banker-type JDM should be treated aggressively. Pulse therapy should be selected as the initial therapy in patients with fulminant-type JDM. 相似文献
3.
TOSHIYUKI YAMAMOTO TATSUYA KOEDA AKIKO TAMURA HIROSHI SAWADA IKUO NAGATA NAOKI NAGATA TAKASHI ITO YASUYUKI MIO 《Pediatrics international》1996,38(6):689-691
A female infant with 21 trisomy syndrome associated with congenital chylothorax was reported. She was born at a gestational age of 34 weeks by Cesarean section because of fetal hydrothorax and hydrops fetus, confirmed by ultrasonography at 32 weeks. Emergent resuscitation and immediate thoracentesis were performed soon after birth. After beginning breast feeding, the serous pleural fluid became opalescent and a diagnosis of congenital chylothorax was made. Feeding was changed to medium-chain triglyceride (MCT) feeding and the production of pleural effusion disappeared after thoracentesis was performed several times. Accumulating evidence suggested that MCT feeding and intermittent thoracentesis under echo guide were effective. Some reports on patients, including this one, suggest that there may be more patients with 21 trisomy associated with congenital hydrothorax. Therefore, congenital hydrothorax might be listed as a complication of 21 trisomy. 相似文献
4.
MASAJI KIKUKAWA YASUYUKI OKAMOTO HIROSHI NAKANO 《Journal of gastroenterology and hepatology》1998,13(6):651-655
: Maintaining high levels of intracellular cyclic AMP (cAMP) is known to inhibit the growth of various proliferating cells including hepatocytes. We show here that transient (30 min) elevations of cAMP induced by addition of 8-bromo-cAMP (1 mmol/L) to rat hepatocytes in primary culture at three time points (12h, 16h and 20h) after seeding stimulated DNA synthesis. Sustained levels of cAMP stimulated DNA synthesis to a lesser degree at a lower concentration (1 μmol/L), but inhibited it at concentrations higher than 100μmol/L. We also determined cyclin-dependent kinase 2 (cdk2) activity in the hepatocytes during this incubation period. The transient addition of 8-bromo-cAMP at the late G1 phase increased cdk2 activity. This suggests that transient cAMP elevation in hepatocytes at the late G1 phase has a growth stimulation effect. Up-regulation of cdk2 activity may have a role in this process. 相似文献
5.
THEODORE J. NITZ YASUYUKI SHIMOHIGASHI TOMMASO COSTA HAO-CHIA CHEN CHARLES H. STAMMER 《Chemical biology & drug design》1986,27(5):522-529
The dehydrophenylalanine4-enkephalin having the E-configuration (ΔEPhe; phenyl and C = O, cis) was prepared by photoisomerization of the Z-isomer with 3100 Å light, followed by reversed-phase HPLC separation of the resulting mixture of the Z- and E-isomers. In the radioligand receptor binding assays, the E-isomer of [D-Ala2, ΔPhe4, Leu5]enkephalin exhibited an extremely diminished affinity as compared with the Z-isomer, namely 150–260-fold loss of affinity for the delta and mu opiate receptors. The results indicate that the interrelationship of the Tyr1 and Phe4 residues in the enkephalin molecule seems to be of great importance in receptor recognition. 相似文献
6.
MICHIO KONDO HIROAKI KODAMA TOMMASO COSTA YASUYUKI SHIMOHIGASHI 《Chemical biology & drug design》1986,27(2):153-159
A cystamine-enkephalin dimer, containing two molecules of [D-Ala2, Leu5] enkephalin cross-linked at the COOH-terminal leucine residue with cystamine, (NH2-CH2-CH2-S-)2, has been synthesized in order to examine directly the dimerization effect of an enkephalin molecule on the opiate receptor interactions. In a comparison of potencies against [3H]-[D-Ala2,D-Leu5]enkephalin (3H-DADLE) and [3H]-[D-Ala2,MePhe4,Gly-ol5]enkephalin (3H-DAGO) as delta and mu tracers, respectively, enkephalin dimer showed a very high affinity, especially for the delta opiate receptors. Dimer was almost threefold more potent than DADLE, which is one of the most utilized delta ligand to date. When the binding affinity of cystamine-dimer was compared with that of its reduced thiol-monomer, namely [D-Ala2,Leu5, cysteamine6]enkephalin, the increment in affinity was four to fivefold for both delta and mu receptors. The results strongly indicate that the dimeric enkephalin is more potent presumably due to the simultaneous interaction with the two binding sites of the opiate receptors. 相似文献
7.
Malignant transformation of renal angiomyolipoma 总被引:8,自引:0,他引:8
NOBUYOSHI TAKAHASHI RYUJI KITAHARA YASUYUKI HISHIMOTO AKIMITSU OHGURO YASUHIRO HASHIMOTO TADASHI SUZUKI 《International journal of urology》2003,10(5):271-273
In the present paper, two cases of malignant transformation of renal angiomyolipoma without tuberous sclerosis are reported. Pathological examination revealed that, in both cases, in addition to the areas affected by typical angiomyolipoma, there were areas that contained elevated numbers of perivascular epithelioid cells with prominent nuclear pleomorphism. Immunohistochemical examination revealed that both cases were negative for keratin and epithelial membrane antigen, but were positive for the melanogenesis-related marker HMB-45. Metastatic diseases appeared 40 months after radical nephrectomy in the first case and 18 months in the second case. 相似文献
8.
HIROAKI KODAMA YASUYUKI SHIMOHIGASHI TOMMASO COSTA MICHIO KONDO 《Chemical biology & drug design》1988,32(1):41-46
For the elucidation of structural elements in the opiate receptors, a thiol-containing enkephalin analog, [d -Ala2, cysteamine5]enkephalin, and its dimeric analog were synthesized and evaluated in the radio-ligand receptor binding assays using rat brain membranes. The dimeric analog was very potent in both delta and mu assays. Comparison of receptor affinities of the thiol-containing enkephalin with those of standard mu or delta receptor specific ligands suggested that the mu receptor contains an essential thiol group which may interact with the thiol group at the C-terminus of the enkephalin analog. It also appears that no metal-ion site, postulated for the delta receptors, is present in the delta binding site. 相似文献
9.
The Autonomic Control of the Transmural Dispersion of Ventricular Repolarization in Anesthetized Dogs 总被引:4,自引:0,他引:4
MANABU TAKEI M.D. YASUYUKI SASAKI M.D. TAKANORI YONEZAWA M.D. MANOJ LAKHE M.D. MASAKAZU ARUGA M.D. KENDO KIYOSAWA M.D. 《Journal of cardiovascular electrophysiology》1999,10(7):981-989
INTRODUCTION: The initiation of ventricular arrhythmias is in part associated with autonomic nervous tone. We investigated the effects of sympathetic and parasympathetic stimulation on the monophasic action potentials (MAPs) of the epicardium (EPi), mid-myocardial (M) region, and endocardium (Endo) in vivo. METHODS AND RESULTS: In 12 mongrel open chest anesthetized dogs, both sides of the cervical vagus nerves and stellate ganglia were crushed with a tight ligature. Right atrial pacing at 600 msec cycle length was begun after the sinus nodal area had been crushed. MAPs from the M region were measured by two needle electrodes that were supported by a W-shaped plastic frame. The epicardial, M region, and endocardial MAP durations at 90% repolarization (MAPD90) were 287+/-7, 315+/-7, and 290+/-8 msec, respectively. The MAPD90 from M cells was longer than that from Epi or Endo. Sympathetic stimulation shortened MAPD90 more in the M region (53+/-4 msec) than that in the Epi (27+/-3 msec) or Endo (26+/-4 msec). The transmural dispersion of repolarization during sympathetic stimulation was shorter than that of the control. Parasympathetic stimulation did not significantly affect any of the MAPD90 values. Simultaneous sympathetic and parasympathetic stimulation produced changes not significantly to those produced by sympathetic stimulation alone. CONCLUSION: Our results suggest that sympathetic activity can reduce transmural dispersion of repolarization under autonomic control in the canine heart under baseline conditions. 相似文献
10.
MICHIHISA ZENMYO SETSURO KOMIYA RIKIMARU KAWABATA YASUYUKI SASAGURI AKIO INOUE MINORU MORIMATSU 《The Journal of pathology》1996,180(4):430-433
The purpose of this study was to investigate the mechanism of cell death in chondrocytes of the growth plate. In the degenerative chondrocyte zone of the growth plate, apoptotic chondrocytes were defeated by the in situ nick end labelling method, by DNA analysis in agarose gel, and by electron microscopy. The results of the in situ nick end labelling method and the occurrence of a ladder pattern of DNA in agarose gel analysis indicated the activation of endogenous endonucleases, resulting in DNA fragmentation. Electron micrographs showed the early morphological changes associated with apoptosis. This report presents both morphological and biochemical evidence for apoptosis in the terminal hypertrophic chondrocytes of the growth plate. These data suggest that apoptosis of degenerative chondrocytes may play an important role in the control of normal and pathological endochondral ossification. 相似文献