毛细管区带电泳法检测甲氨蝶呤方法的探讨

目的 :建立毛细管区带电泳分离甲氨蝶呤的方法。方法 :甲氨蝶呤标准品溶解后直接进样电泳分离。电泳分离条件 :未涂层石英毛细管 5 0cm× 5 0 μm ;运行缓冲液为 5 0mmol/L磷酸盐溶液 (pH3 0 0 ) ;操作电压 2 5kV ;压力进样 0 5psi,5s ;柱上紫外 2 14nm检测。结果 :甲氨蝶呤在 2 0min内获得理想的电泳分离。浓度在 0 99～ 99 0 0 μg/ml范围内线形关系良好(r =0 9999,n =5 ) ,回收率为 96 80 % ,日内及日间精密度 (RSD)均小于 6 %。结论 :毛细管区带电泳法分离甲氨蝶呤 ,方法准确可靠 ,操作简便易行。     

高效毛细管电泳分离测定颠茄制剂中莨菪类生物碱

选择毛细管区带电泳分离模式，以麻黄素为内标建立了适合莨菪类生物碱阿托品、东莨菪碱、山莨菪碱、樟柳碱化化分离与颠茄酊剂及片剂定量分析的高效毛细管电泳方法。电泳分离条件：毛细管40c×50μm，运行缓冲液（pH8.0）50mmol／L磷酸盐溶液-四氢呋喃（9:1），电迁移进样5kV×5s，运行电压11kV（＋）→（－），柱上检测UV200um，0．02AUFS，毛细管柱温24℃。本法简便、快速、重现性好，可用于含莨菪类生物碱中药制剂的质量控制。     

头孢克洛人体尿药浓度的毛细管电泳分析

目的 :建立适合国人尿液中头孢克洛分离分析的毛细管电泳方法。方法 :用重蒸馏水多倍稀释尿液后直接进样电泳分析。电泳分离条件为 :未涂层毛细管 48cm× 5 0μm ;运行缓冲液为 5 0 m mol/ L磷酸二氢钠 - 12 .5 mm ol/ L硼砂 (p H 8.0 8) ;操作电压 2 0 k V(+ )→ (- ) ;压力进样 8.2 74× 10 4Pa· s;柱上紫外 2 6 5 nm检测。 结果 :头孢克洛和内标物头孢拉定在 6 .5min内获得了理想的分离。尿药浓度在 2 .5～ 2 5 0 .0μg/ ml范围内线性关系良好 (r =0 .9991,n =5 ) ,绝对回收率大于95 .5 % ,日内及日间精密度均小于 8.0 %。 结论 :毛细管电泳应用于健康志愿者口服头孢克洛普通胶囊后尿药浓度的动态分析 ,方法准确可靠 ,操作简便易行     

高效毛细管电泳法测定血清中头孢他啶的含量

采用高效毛细管电泳法测定血清中头孢他啶的含量。毛细管 6 0cm× 75 μm ;运行缓冲液 2 5mmol/L四硼酸钠 (pH8 2 ) ,高压进样 5s,分离电压 2 0kV ,温度为 2 5℃ ,检测波长为 2 5 4nm ,地塞米松磷酸钠为内标。头孢他啶在 0 .6 2 5～ 2 0 μg/mL浓度范围内线性关系良好 (r =0 .9997) ,平均回收率为 98.81 % (n =5 ,RSD =1 .88% )。     

红霉素微球中红霉素的高效毛细管电泳-电导法测定

建立了红霉素微球中红霉素含量测定的高效毛细管电泳 -电导法。测定条件为 :未涂层弹性融硅石英毛细管柱 (40cm×75μm)为分离通道 ,Tris-H3 BO3 为电泳介质 (30mmol LTris,5mmol LH3 BO3 ) ,重力方式进样 ,室温下 2 6kV恒压电泳分离 ,电导检测。红霉素在 0 .946～1 1 .352mg mL浓度范围内线性关系良好 ,平均回收率 92 .6 % ,RSD为 2 .1 6 %。     

儿茶酚胺和二羟苯乙酸的区带毛细管电泳测定法

目的　建立儿茶酚胺和二羟苯乙酸 (Dopac)的毛细管电泳检测方法。　方法　区带毛细管电泳测定儿茶酚胺和 Dopac分离缓冲液为含 5 7mm ol/ L Na H2 PO4,0 .2 m mol/ L EDTA,1.2 mmol/ L HSA和 8%甲醇的缓冲溶液 p H =3.2 ,紫外检测波长为 2 0 0 nm。　结果　儿茶酚胺和 Dopac的分离效果和重现性良好 ,灵敏度可达 5× 10 - 9g/ ml,在 0 .0 1～ 0 .32 μg/ m l浓度范围线性关系好。　结论　本方法用于儿茶酚胺和 Dopac的分析具有快速、简便、耗材少的优点。     

急性脑卒中患者血浆胰岛素样生长因子1与胰岛素抵抗的关系

目的　探讨急性脑卒中患者血浆胰岛素样生长因子 1(IGF 1)及其结合蛋白 3(IGFBP3)水平的变化 ,及两者与胰岛素抵抗 (IR)有关的血浆胰岛素 (FINS)水平及其敏感性系数 (ISI)的相关性。方法　应用放射免疫法检测了 4 0例急性脑出血 (ACH)患者、34例脑梗死 (ACI)患者、脑卒中恢复期组30例和正常对照组 30名的血浆IGF 1、IGFBP3、FINS水平 ,同时测定空腹血糖 (GS)水平。结果　 (1)ACI和ACH组血浆IGF 1、IGFBP3、ISI显著低于正常对照组和脑卒中恢复期组 (分别为ACI:17 3mmol/L± 7 0mmol/L ,72 9mmol/L± 11 4mmol/L ,- 4 2± 0 3;ACH :14 4mmol/L± 5 8mmol/L ,5 7 8mmol/L± 17 2mmol/L ,- 4 7± 0 6 ,恢复期组 :2 8 8mmol/L± 13 6mmol/L ,87 4mmol/L± 18 3mmol/L ,- 3 9±0 4 ;正常对照组 :31 5mmol/L± 18 9mmol/L ,93 1mmol/L± 2 8 0mmol/L ,- 3 6± 0 3;F分别为 18 3,2 0 2 ,17 2 ,P <0 0 1;GS、FINS显著高于正常对照组 (ACI组 :6 1mmol/L± 1 0mmol/L ,15 7mIU/L±3 1mIU/L ;ACH组 :6 4mmol/L± 2 1mmol/L ,19 7mIU/L± 9 6mIU/L ;正常对照组 :4 8mmol/L± 0 5mmol/L ,10 6mIU/L± 3 2mIU/L ;F分别为 8 5 ,10 6 ;P <0 0 1) ;(2 )ACI组和ACH组血浆IGF 1与IGFBP3水平呈正相关 (P     

多脏器功能衰竭患者并罕见心肌酶谱增高1例报告

1　病例病例 ,男 ,32岁 ,未婚 ,无业 ,有 15年反复上腹痛史。于 2 0 0 2年 5月 11日 ,因恶心 ,呕吐咖啡色液体 15 0 0ml,无黑便 ,嗜睡 1天住入我院消化科。查体温 (T) 36 .4℃ ,血压 (BP) 10 0 / 6 0mmHg。皮肤较干燥 ,两肺未及罗音。二对半检查 :HBsAg(+) ,HBeAb(+) ,HBcAb(+)。白细胞 (WBC) 13.1×10 9/L ,N 0 .75 ,L 0 .2 5 ,Hb 133g/L ,Plt 32 7× 10 9/L ,K+ 5 .1mmol/L ,Na+ 135mmol/L ,Cl- 6 8mmol/L ,二氧化碳结合率 (CO2 CP) 14mmo/L ,Ca2 + 1.2 7mmol/L ,尿素氮 (BUN) 4 3mmol/L ,血清肌酐 (Scr) 5 84 μmmol/L…     

胶束电动毛细管色谱法对三磷酸腺苷及其分解产物的分离分析

对ATP(adenosine triphosphate)及其分解产物ADP(adenosine diphosphate)、AMP(adenosine monophcsphate)等采用胶束电动毛细管色谱法进行分离分析。电动色谱系统为:毛细管柱57 cm×45 μm(I.D),石英毛细管,UV紫外检测器(波长260 nm),磷酸盐缓冲溶液(Na_2HPO_4)5 mmol/L,十二烷基硫酸钠(SDS)50 mmol/L(pH=9.06)。获得了良好的分离,为ATP的定量建立了良好的分析系统。     

毛细管电泳法测定金芪降糖片中小檗碱的含量

目的 :采用毛细管电泳法快速地对金芪降糖片中 3种生物碱进行分析并测定其中盐酸小檗碱的含量。探讨有机改性剂的加入对分离的影响以及缓冲液的重复使用对定量分析产生的影响。方法 :毛细管电泳 ,未涂层石英毛细管 (5 0cm× 10 0 μm) ,以 4 0mmol/L磷酸氢二钠—甲醇 (6 5∶35 )为缓冲液 ,2 5 4nm为测定波长 ,测定盐酸小檗碱含量。结果 :在 12min内测定盐酸小檗碱 ,盐酸小檗碱平均含量为 1 5 2 % (g/g) ;线性范围 0 0 1～ 0 2 0mg/ml;r=0 9999。结论 :该方法可做为金芪降糖片中盐酸小檗碱含量测定方法之一。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.