免疫载药脂质体治疗小鼠泡球蚴

目的：观察载阿苯达唑（ABZ）的抗泡球蚴抗体的免疫脂质体对小鼠继发性泡球蚴病的疗效。方法：观察游离的、脂质体运载与免疫脂质体运载的ABZ（12．5、25和50mg／kg）对小鼠腹腔泡球蚴增殖的抑制。结果：免疫脂质体治疗抑制率高于脂质体，后者又高于游离ABZ，高剂量时3组抑制率分别为87．8％、65．2％和17.7％，组间比较差异显著（P＜0.01）；肝和腹腔泡球蚴摄取免疫脂质体的量比单纯脂质体高2．4倍（P＜0．01）。结论：免疫脂质体作载体可明显提高ABZ对泡球蚴病的疗效。     

更正

目的：探讨利培酮口服液治疗精神分裂症患者的依从性。方法：把符合CCMD-3诊断标准的精神分裂症患者100例，随机分成两组，分别给予利培酮口服液（B组）和阿立哌唑（A组）治疗8周，采用简明精神症状量表（BPRS）评定疗效，副反应量表（TESS）评定副反应。比较两组的服药依从性。结果：治疗8周后两组疗效差异不显著，B组药物完全依从占67．2％，显著高于A组的42.1％。不依从占3．4％，则显著低于A组的32．9％。利培酮的不良反应以锥体外系为主，阿立哌唑则以嗜睡为主，但易缓解。结论：利培酮口服液能更好地提高治疗的依从性。     

阿苯达唑脂质体治疗复杂肝泡型包虫病的初期临床观察

目的 初步评价阿苯达唑脂质体口服液治疗复杂肝泡型包虫病的临床疗效.方法 回顾性分析2000～2009年阿苯达唑脂质体口服液治疗的26例复杂肝泡型包虫病例的临床资料.26例患者中13例行单纯药物化疗,13例行姑息手术联合药物化疗,比较二者生存率差异.结果 2种治疗方式5年生存率差异无统计学意义(P>0.05),19例长期化疗随访病例健康存活.结论 阿苯达唑脂质体口服液治疗肝泡型包虫病是安全有效的,可以长期使用.同时单纯阿苯达唑脂质体口服液的化疗与姑息手术联合化疗治疗复杂病例的治疗方式可能无临床差异,但无论是否手术,有效长程的化疗都应作为必要的治疗选择.     

Clinical efficacy of albendazole emulsion in treatment of 212 cases of liver cystic hydatidosis

目的：验证新剂型阿苯达唑乳剂对肝囊型包虫病患的临床疗效。方法：对２１２例肝囊型包虫病患用阿苯达唑10mg/Kg/d和12.5mg/Kg/d两种剂量进行治疗。服药3个月复查一次为一个疗程,各疗程之间不断连续用药。以B超影象特征为主判定疗效。观察不同疗程的效果。以停药时的检查结果为近期疗效。停药后随访１－４年的结果为远期疗效。结果：两个剂量组共212例患的平均近期疗效为：治愈率74.5％,有效率为99.1%,无效率为0.9%。平均远期疗效为：治愈率为83.1%,有效率为８９.３%,无效率为0.6%,复发率为10.2％。以12.5mg/Kg/d连续治疗9个月的疗效最好。复发病人再治疗的效果良好。结论：阿苯达唑乳剂对肝囊型包虫病的临床疗效超过当前包虫病药物治疗的最好水平。且疗效稳定可靠,不良反应轻微。可做为包虫病首选治疗方法。     

阿苯达唑脂质体口服液的临床疗效及药物副作用

目的探讨阿苯达唑脂质体口服液的临床疗效及药物副作用。方法收集我院100例包虫病患者,随机分为观察组和对照组,均为50例。对照组实施阿苯达唑片治疗,观察组实施阿苯达唑脂质体口服液的治疗,观察临床疗效和药物副作用。结果观察组患者的治疗显效率和总有效率均明显高于对照组,并发症发生率明显低于对照组。结论阿苯达唑脂质体口服液能够明显提升临床治疗效果,同时药物副作用明显低于常规阿苯达唑片,值得推广应用。     

小儿热感宁口服液治疗小儿上呼吸道感染的临床研究

目的 评价小儿热感宁口服液（XRK）治疗小儿上呼吸道感染（上感）的疗效及安全性。方法 120例上感患儿随机分成观察组（60例）和对照组（60例）,观察组口服XRK的剂量为：1岁以内2．5－5ml,1－3岁5－10ml,3－7岁10－15ml,7－13岁15-20ml,1日3－4次,首剂加倍,3日1疗程。记录患儿药前后的体温、临床症状及不良反应,并按标准作出疗效评定,对照组口服维C银翘冲剂治疗,并对两组进行疗效比较。结果 观察组显效44例（73．3％）,好转8例（13．3％）,效8例（13．3％）,总有效率为86．6％,未发现与本药治疗有关的不良反应。对照组显效26例（43．3％）,好转18例（30％）,无效16例（26．7％）,总有效率为73．3％。两组显效率比较P＜0．01。结论 小儿热感宁口服液是一种临床治疗小儿上感的安全有效的中西药复方制剂。     

中西医结合治疗异位妊娠效果观察

目的：寻找一种简单、有效、副反应小、不影响生育功能的药物治疗异位妊娠方法。方法：将248例异位妊娠患者随机分为3组，A组中西医结合组78例（MTX加中药联合治疗）；B组中药治疗组90例，C组单纯MTX治疗组3组80例，对3组的疗效和副反应进行比较。结果：A组包块缩小程度、血B-hCG下降程度显著，住院天数缩短；3组治疗成功率A组89．7％、B组61．1％，两者相比差异有统计学意义（P〈0．05），与C组82．5％比较，差异无统计学意义（P〉0．05）；A组副反应发生率6．4％、C组33．8％，两者差异有统计学意义（P〈0．05）。结论：中西医结合治疗异位妊娠疗效高，副作用小，可作为临床保守治疗的首选方案。     

六君子汤联合阿苯达唑治疗包虫病疗效观察

目的对2011-2012年金塔县实施《甘肃省包虫病流行情况调查技术方案》确诊的36例包虫病患者的病情进行回顾性分析。探讨包虫病的发病趋势、流行特征及治疗方法,为制定包虫病治疗方案提供临床经验和科学依据。方法对本县24057例健康人群依据《包虫病诊断标准》（WS257—2006）,采用B超影像学检查方法进行普查,对疑似者同时采用血清学方法进行辅助检查,将确诊的36例患者分为联合用药组（六君子汤＋阿苯达唑治疗）20例,对照组（阿苯达唑治疗）16例并进行疗效观察。结果中药六君子汤联合阿苯迭唑治疗总有效率达100．00％,应用阿苯迭唑治疗总有效率为81．25％,前者明显优予后者,差异有显著性（P〈0．05）。结论六君子汤联合阿苯迭唑治疗包虫病,能有效控制包虫病的蔓延和扩散,疗效显著。适合基层医院推广和应用,同时也为今后制定包虫痛治疗方案提供了临床经验和科学依据。     

L—谷氨酰胺胶囊治疗消化性溃疡的临床研究

目的：评价L－谷氨酰胺治疗消化性溃疡的临床疗效及安全性。方法：采用随机双盲双模拟药物平行对照试验观察40例病人，分试验组（A组）20例，十二指肠球部溃疡12例，占60％,，胃溃疡8例占40％；对照组（B组）20例，十二指肠球部溃疡13例，占65％，胃溃疡7例占35％，A组用L－谷氨酰胺胶囊，每次2粒（500mg),每日3次，我后服用，十二指肠溃疡疗程6周，胃溃疡8周。B组用替普瑞酮，每次2粒，用法及疗程用A组。结果：总有效率十二指肠溃疡试验组91．7％，对照组92．3％，差异无显著性。胃溃疡试验组87．5％，对照组85．6％，差异无显著性（P＜0．05），不良反应发生率10％，症状轻微。结论：L－谷氨酰胺治疗PU临床疗效确切。     

经皮细针穿刺治疗肝囊型包虫病22例初步临床观察

１９９６年１月至１１月期间,我科依照ＷＨＯ有关人体包虫病治疗指导纲要推荐的穿刺治疗包虫病方法（简称ＰＡＩＲ）,在麻醉科医师监护下,由Ｂ超定位引导细针（内径０．６～１．３ｍｍ）穿刺治疗了２２例肝囊型包虫病患者共计２６个包虫囊。经过７～１８个月的临床随访,结果：（１）包虫囊消失或小于１ｃｍ者占３１％,囊包虫缩小６５％以上者３１％包虫囊缩少４０％～５０％者占３８％;（２）其中５例患者置管外引流２周至３个月不等,拔管后情况良好。４例穿刺证实为包虫术后残腔。穿刺治疗前后所有患者均给予口服丙硫咪唑（ａｌｂｅｎｄａｚｏｌｅ）,每日每公斤体重１０～１５ｍｇ。穿刺前用药３～５ｄ,穿刺后酌情用药１～３个月,每３个月进行Ｂ超随访至少２年。初步结果表明：在严格掌握穿刺适应证条件下,ＰＡＩＲ可作为一种经济、有效、创伤小的治疗手段,尤其对有多次手术史的肝包虫病患者更具优越性。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.