鼻咽癌患者血清sICAM-1和SAA1及HSP70检测临床意义分析

目的:探讨可溶性细胞间黏附分子-1(sICAM-1)、血清淀粉样蛋白A1 (SAA1)和热休克蛋白70 (HSP70)在鼻咽癌患者血清中的表达水平及其诊断价值.方法:用酶联免疫吸附法(ELISA)对30例鼻咽癌转移患者,27例鼻咽癌未转移患者和30名正常人的血清sICAM-1、SAA1和HSP70进行检测.结果:血清sICAM-1、SAA1和HSP70含量在鼻咽癌组与正常对照组、鼻咽癌淋巴结转移组与无淋巴结转移组之间差异均有统计学意义,P值均＜0.01.血清sICAM-1和HSP70水平在不同TNM分期中差异有统计学意义,随着TNM分期的提高,其血清水平逐步提高,P＜0.01.而且这3种蛋白质在鼻咽癌患者血清中均具有相关性,即在鼻咽癌患者血清中,这3种蛋白质中的某一种蛋白质含量升高,则另外2种蛋白质的含量也会上升,P＜0.01.结论:经双向电泳和质谱鉴定出的蛋白质在3组标本中确实具有差异,且有一定意义.sICAM-1和HSP70的表达随着鼻咽癌的发展逐渐增强,可能反映鼻咽癌的进展.动态检测鼻咽癌患者血清sICAM-1、SAA1和HSP70的水平,可作为鼻咽癌诊断的一个重要指标,有一定的临床价值.     

TGF-β1、Cyclin E蛋白阳性在原发性胆囊癌诊治中的意义

目的 :检测原发性胆囊癌中TGF β1、CyclinE蛋白阳性 ,在其癌发生、发展过程中的表达特点及其在诊治中的临床意义。方法 :应用免疫组化S P法检测 36例原发性胆囊癌中TGF β1、CyclinE的阳性率 ,并以同期 2 0例慢性胆囊炎作对照。结果 :①TGF β1蛋白阳性率为 6 3.89% (2 3/ 36 )显著高于其相应良性对照组 10 % (2 / 2 0 )。Cy clinE蛋白阳性率为 4 7.2 2 % (17/ 36 )显著高于其相应良性对照组 10 % (2 / 2 0 )。②TGF β1蛋白在有淋巴结或远处转移、NevinⅣ～Ⅴ期患者中阳性率 (79.17% )明显增高于无转移、NevinⅠ～Ⅲ期的患者 (33.33% )。CyclinE在低分化、有淋巴结或远处转移阳性率 (84 .6 2 %、6 2 .5 0 % ) ,明显高于高中分化、无转移 (2 6 .0 9%、16 .6 7% )。③CyclinE与TGF β1两者呈正相关 (P <0 .0 5 )。④TGF β1阳性患者生存率明显低于TGF β1阴性患者。CyclinE阳性患者生存率明显低于CyclinE阴性患者。结论 :①在原发性胆囊癌细胞中TGF β1、CyclinE蛋白阳性率表达上调 ,且在有淋巴结或远处转移、NevinⅣ～Ⅴ期患者中更为明显。②联合检测TGF β1、CyclinE蛋白在原发性胆囊癌中阳性有助于反映原发性胆囊癌生物学特性、为预后判断提供参考指标 ,并为探索原发性胆囊癌早期诊断提供实验依据。     

原发性胆囊癌的超声诊断价值

目的　探讨原发性胆囊癌 (PCG)的术前诊断。方法　对近 6年来 2 4例经手术、病理证实为PCG的临床表现和超声图象特征进行回顾性分析。结果　 (1)、2 4例中 ,男 6例 ,女 18例 ,5 5岁以上者占 91.7%。 (2 )、主要临床表现为上腹痛 (91.7% )和黄疸 (4 5 .8% )。 (3 )、声像图显示胆囊壁不均匀性增厚 15例 (62 .5 % ) ,B超诊断符合率 5 8.3 %。结论　我们应重视 5 5岁以上腹痛、黄疸患者的临床表现和胆囊壁不均匀性增厚。超声对PCG术前诊断有一定的价值。     

原发性胆囊癌的影像学诊断

目的　比较超声 ,CT ,PTC和ERCP对原发性胆囊癌的诊断价值。方法　 74例胆囊癌病人术前分别进行了超声 ,CT ,PTC和ERCP检查 ,对这些影像学检查进行回顾性研究分析。结果　超声 ,CT和ERCP对胆囊癌的正确诊断率分别为 80 .3 % ,88.5 5和 2 5 % .7例直径 >3cm的胆囊癌中有 6例CDFI阳性。 4例伴有黄疸的病人行PTC检查的目的主要是为了PTBD减黄。结论　超声是诊断胆囊癌首选的影像学检查方法 ,CDFI在确定胆囊恶性肿瘤时很有帮助。疑似胆囊癌或有转移和进行临床分期时 ,CT较超声更有用。胆囊癌伴有黄疸的病人 ,PTC和ERCP主要以治疗为目的     

原发性胆囊癌nm23-H1和p53基因蛋白的表达及意义

原发性胆囊癌临床过程隐匿，转移早，侵袭力强，预后不佳。如何准确判断其恶性程度和浸润转移倾向，倍受人们关注。nm２３－H１、p５３抑癌基因被认为对恶性肿瘤的发生和转移有抑制作用，且与分级、分期及预后有关。本研究应用免疫组化技术检测了原发性胆囊癌及胆囊息肉样病变nm２３－H１和p５３基因蛋白表达及其与胆囊癌临床病理参数的关系。１材料与方法１．１临床材料及标本４５例胆囊癌为本院存档标本，病理诊断明确，均经１０％福尔马林液固定、石蜡包埋。另收集１５例胆囊息肉样病变作对照。胆囊癌的一些临床病理资料如临床分期、浸…     

原发性胆囊癌中Nrf2的表达及意义

目的:探讨nuclear factor-erythroid 2 p45-related factor 2(Nrf2)在原发性胆囊癌中的表达及其与病理指标的关系.方法:应用免疫组化SP法检测59例原发性胆囊癌中Nrf2的表达.结果:Nrf2呈散在分布的淡黄色或棕褐色颗粒,主要定位于细胞核中,散见于细胞浆中,强阳性表达率分别为72.8%.随着转移程度、Nevin 分级以及病理分级的增加,强阳性表达率增加,且具有统计学差异(P<0.05).Nrf2表达与性别、年龄相关性不明显(P>0.05).结论:Nrf2的高表达参与了原发性胆囊的发生发展,Nrf2与原发性胆囊癌的分化、转移有关.     

非小细胞肺癌患者血清sICAM-1、sVCAM-1水平的临床意义

目的:探讨血清sICAM-1,sVCAM-1水平测定在非小细胞肺癌中的临床意义。方法:应用ELISA法测定43例非小细胞肺癌患者(其中14例为化疗后患者)血清sICAM-1、sVCAM-1的浓度,并与正常人比较。结果:Ⅰ+Ⅱ期、Ⅲ+Ⅳ期肺癌患者sICAM-1的浓度分别为370.65±78.63ng/ml、550.88±343.31ng/ml均明显高于正常组(P<0.01),且Ⅲ+Ⅳ期患者的sICAMV-1与Ⅰ+Ⅱ期比较具有显著性差异(P<0.05),Ⅰ+Ⅱ期、Ⅲ+Ⅳ期肺癌患者sVCAM-1的浓度分别为543.92±142.37ng/ml、885.12±892.12ng/ml均明显高于正常组(P<0.01),且二者之间也存在差异(P<0.05)。14例化疗后的患者sICAM-1、sVCAM-1分别为351.78±161.80ng/ml、418.64±251.64ng/ml均明显低于化疗前(P<0.05,P<0.01)。结论:血清sICAM-1,sVCAM-1水平与肺癌的病情发展相关,并有可能作为肺癌化疗评价疗效的血清学指标之一。     

原发性胆囊癌的诊治

目的 探讨原发性胆囊癌的病因及诊断。方法 回顾性分析我科1986年10月-1999年4月收治的31例原发性胆囊癌病例资料。结果 结石合并率71%（23/31）；B超检出率40%（12/30），CT检出率71.4%(10/14)。结论 胆囊结石，胆囊息肉样病变及胆肠内瘘为主要原因。B超检查有助于早期诊断。加强对高危人群的监测，早期诊断和积极治疗是提高手术根治率及5年生存率的关键。     

原发性胆囊癌22例临床分析

我院1997年6月～2 0 0 3年6月收治的原发性胆囊癌2 2例,总结报道如下。1　临床资料1.1　一般资料2 2例胆囊癌中,男12例,女10例。年龄3 8～73岁,平均5 6 6岁。病程最长5年,最短2d。临床表现为右上腹痛2 0例( 90 % ) ,黄疸6例( 2 7% ) ,发热4例( 18% ) ,恶心8例( 3 6% ) ,消瘦4例     

Circulating intercellular adhesion molecule 1 (ICAM-1), E-selectin and vascular cell adhesion molecule 1 (VCAM-1) in head and neck cancer

The sera from patients with nasopharyngeal carcinoma (n = 30), oral carcinoma (n = 22) and laryngeal carcinoma (n = 22) was extracted before treatment. The concentration of circulating intercellular adhesion molecule 1 (ICAM-1), E-selectin and vascular cell adhesion molecule 1 (VCAM-1) was measured by enzyme-linked immunoassay and compared with those from normal subjects (n = 20). The concentration of circulating ICAM-1, E-selectin and VCAM-1 was significantly increased in nasopharyngeal carcinoma. Correspondingly, VCAM-1 and E-selectin were significantly increased in laryngeal carcinoma, whereas only E-selectin was elevated in oral carcinoma. The concentrations of these adhesion molecules did not significantly differ with respect to the early and late stages of these carcinomas. Elevated levels of soluble adhesion molecules in the sera of cancer patients at three different head and neck regions, although appearing to be implicated in these tumour formations, may be unrelated to tumour progression. © 1999 Cancer Research Campaign     

Levels of soluble intercellular adhesion molecule-1 and total sialic acid in serum of patients with colorectal cancer

BACKGROUND AND OBJECTIVES: Serum soluble intercellular adhesion molecule-1 (sICAM-1) and total sialic acid (TSA) are related to the metastatic potential of cancer cells. The purpose of the present investigation was to determine sICAM-1 and TSA levels in colorectal carcinoma and correlate their levels with the cancer stage. METHODS: The sera from 65 patients with colorectal cancer (18 at Dukes' B, 24 at Dukes' C, 23 at Dukes' D) were extracted before treatment. The concentrations of sICAM-1 and TSA were measured by enzyme-linked immunoassay and the thiobarbituric acid method, respectively, and compared with those from a healthy control group (n = 42). RESULTS: Mean serum sICAM-1 and TSA levels were found to be higher in the total patient group than in the control group (P < 0.0001). The concentrations of sICAM-1 and TSA were significantly higher in patients with Dukes' C and Dukes' D. The correlations between sICAM-1 and TSA became more significant as the stage of the disease increased (r = 0.58, P < 0.05 in Dukes' B, r = 0.88, P < 0.01 in Dukes' C and r = 0.81, P < 0.01 in Dukes' D). CONCLUSIONS: The results of this investigation indicate that sICAM-1 and TSA are the best of the tested markers. These markers should prove useful for monitoring malignant disease stage and for evaluating the effectiveness of various therapeutic approaches for colorectal carcinomas.     

Expression of intercellular adhesion molecule-1 in hepatocellular carcinoma

The expression of intercellular adhesion molecule-1 (ICAM-1) was investigated in frozen sections obtained from 40 resected liver specimens of patients with hepatocellular carcinoma using immunoperoxidase techniques and immunoelectron microscopy. ICAM-1 was expressed in 80% of the HCC specimens on the membrane of cancer cells. In noncancerous regions characterized by cirrhosis in 28 cases and chronic hepatitis in 12 cases, ICAM-1 was rarely expressed on hepatocytes but was expressed mainly on the endothelium of portal vessels and sinusoidal lining cells. These results suggest that expression of ICAM-1 in hepatocellular carcinoma may be induced by malignant transformation of hepatocytes. © 1993 Wiley-Liss, Inc.     

Serum levels of intercellular adhesion molecule ICAM-1 and E-selectin in advanced stage non-small cell lung cancer

Cell adhesion is a basic count in inter- and intra-cellular communication and plays an important role in tumor progression. This study was conducted to investigate the serum levels of intercellular adhesion molecule (ICAM-1) and E-selectin in patients with advanced stage non-small cell lung cancer (NSCLC) and the relationships with known prognostic parameters and therapy. These serum factors were measured of 57 NSCLC patients pathologically verified before and after chemotherapy in comparison with 24 healthy controls by using ELISA method. Serum levels of ICAM-1 were increased significantly in NSCLC patients compared with the healthy controls (P = 0.006). However, serum E-selectin levels were not significantly different from healthy control groups (0.643). No statistically significant relationships were found between investigated all serum parameters and various characteristics of patients, and the diseases such as stage and tumor burden. Likewise, we also found no correlation between serum ICAM-1 and E-selectin (P = 0.78). We found that serum ICAM-1 levels were decreased owing to the chemotherapy effect, independently from chemotherapy response. However, serum E-selectin levels were not changed by the chemotherapy effect. The median survival of all patients was 11.9 months and 1-year survival rate was 47.6%. We found that patients performance status (P = 0.013), age (P = 0.015), and weight loss (P = 0.007) were prognostic factors for survival. Serum E-selectin levels showed a trend (P = 0.08) related to worse prognosis, however serum ICAM-1 levels were determined as ineffective on survival (P = 0.11). Multivariate analysis revealed that only weight loss (P = 0.005) and E-selectin levels (P = 0.002) remained as an independent prognostic factor for survival in patients with advanced NSCLC. In conclusion, our data suggest that higher serum ICAM-1 can be useful for diagnosis while E-selectin levels have prognostic significance and could be a potential prognostic factor in NSCLC patients.     

Serum levels of the soluble adhesion molecules in patients with malignant melanoma

The incidence of malignant melanoma has been steadily increasing over the past decades. CD 44 is a transmembrane glycoprotein which is implicated in a number of adhesive and migratory events. Downregulation of CD 44 is implicated in the metastatic process. P-Selectin is a member of the selectin family of cell surface molecules. The levels of P-Selectin in biological fluids may be elevated in subjects with a variety of pathological conditions. In malignant melanoma, elevation of the plasma level of soluble intercellular adhesion molecule-1 (sICAM-1) has been associated with a reduction in disease-free survival. This study was performed to investigate the differences in the serum concentrations of the adhesion molecules in patients with malignant melanoma.The study group consisted of 52 patients with malignant melanoma and 20 healthy subjects. No meaningful difference was observed for P-selectin and sICAM 1 levels. A statistically significant decrease was observed in the cancer patients for serum CD 44 levels.     

肺癌患者血清可溶性细胞间粘附分子-1水平及其意义

目的 动态观察肺癌患者血清可溶性细胞间粘附分子-1(sICAM-1)水平,明确sICAM-1在肺癌发展及预后中的意义.方法 选取22例未经任何治疗、组织学类型明确的肺癌,在动脉灌注化疗前、化疗后2周及8周取血,以ELISA法测定血清sICAM-1水平.结果 治疗前,肺癌组血清sICAM-1水平显著高于正常对照组[(348.4±101.7)mg/L vs(171.3±34.2)mg/L,P＜0.01],血清sICAM-1水平与原发灶大小呈正相关(r=0.6014,P＜0.01);血清sICAM-1水平＞300mg/L组与＜300 mg/L组相比,前者的生存率显著低于后者(26.7%vs 85.7%,P＜0.05).治疗有效组化疗后2周sICAM-1水平显著低于化疗前[(248.6±46.7)mg/L vs(377.4±48.7)mg/L,P＜0.01],而治疗无效组,化疗前后sICAM-1水平变化不大(P＞0.05).结论 肺癌患者血清sICAM-1水平与体内肿瘤负荷、临床病期及癌细胞类型有关,血清sICAM-1水平可作为疗效评估及预后的指标之一.     

Differential levels of soluble intercellular adhesion molecule-1 (sICAM-1) in early breast cancer and benign breast lesions

To date, no soluble markers can discriminate benign from malignant breast lesion; therefore, to assess the diagnostic potential of circulating intercellular adhesion molecule-1 (sICAM-1), serum concentrations of sICAM-1 were quantitated in 230 consecutive patients that underwent surgery for breast neoplasias, utilizing an enzyme-linked immunosorbent assay. Histological diagnosis revealed that 177 patients had breast cancer and 53 had a benign breast disease. In the cancer patient group, 90 subjects had pTl tumors without (pT1N0M0, n=46) or with (pT1N1M0, n=41; pT1N2M0, n=3) regional lymph node metastases. Mean levels of serum sICAM-1 of patients with pT1 breast cancer, without or with regional lymph node involvement, were significantly (P<0.05) higher than those of patients with benign breast lesions and of 49 age-matched control subjects. Elevated levels of serum sICAM-1 were detected in 27/90 (30%) pTl breast tumors and in 1/53 (2%) benign breast lesions; thus, among subjects with high levels of sICAM-1, 96% had breast cancer. No significant correlation was found between levels of serum sICAM-1 and breast cancer progression. These observations, altogether, suggest that in the presence of a suspicious breast neoplasm the quantitative analysis of serum sICAM-1 can orient clinical diagnosis towards malignancy.     

原发性肝癌患者血清可溶性细胞间粘附分子-1的测定及其临床意义

目的：探讨血清可溶性细胞间粘附分子－1（sICAM-1)与原发性肝癌发展，转移，疗效及预后的关系。方法：采用ELISA法检测72例原发性肝癌患者治疗前后，12例良性肝肿瘤患者治疗前血清中sICAM-1的水平，再进行比较分析。结果：原发性肝癌患者血清sICAM-1水平高于正常对照组及良性肝肿瘤患者（P＜0．01），且与肿瘤直径，有无腹腔积液，有无肝外转移，临床分期及治疗效果有关。肿瘤越大，血清sICAM-1水平越高（P＜0．01），有腹腔积液者比无腹腔积液者高（P＜0．05），有肝外转移者比无肝外转移者高（P＜0．05），Ⅲ期患者比Ⅱ期者高，Ⅱ期者又比I期者高（P＜0．05或P＜0．01），治疗前比治疗后高（P＜0．01），而正常对照组与良性肝肿瘤患者血清sICAM-1水平无显著性差异（P＞0．05），结论：原发性肝癌患者血清siCAM-1水平在一定程序上可以反映肝癌发展，转移情况及治疗效果，有可能成为预测肝癌转移，复发及疗效的指标。     

血清细胞间黏附分子-1 在胰腺癌诊断和预后评估中的价值

[摘要] 目的：探讨血清细胞间黏附分子-1（intercellular adhesion molecule-1,ICAM-1）在胰腺癌诊断和预后评估中的价值。方法：选取2015 年4 月至2017 年12 月在湖北省肿瘤医院肝胆胰外科就诊的胰腺癌患者80 例（胰腺癌组）、胰腺良性疾病患者40例（良性疾病组）及同期健康体检者30 例（对照组）。分别检测3 组人群血清ICAM-1 和CA19-9 水平;采用受试者工作特征曲线（ROC）分析ICAM-1 对胰腺癌的诊断特性,采用COX回归模型分析血清ICAM-1 与胰腺癌患者预后是否独立相关。结果：胰腺癌组ICAM-1 和CA19-9 水平明显高于良性疾病组和对照组（均P<0.01）,良性疾病组CA19-9 水平明显高于对照组（P<0.01）。血清ICAM-1、CA19-9 以及两者联合的曲线下面积（AUC）为0.732（95%CI：0.658~0.807,P=0.000）、0.691（95%CI:0.620~0.762, P=0.000）、0.747（95%CI ：0.674~0.821,P=0.000）;ICAM-1与CA19-9之间呈显著正相关（r=0.472,P=0.000）。血清ICAM-1<2 308 U/ml患者的生存时间明显长于≥2 308 U/ml 的患者（χ2=28.357,P=0.000）;ICAM-17≥2 308 U/ml 是患者预后的独立影响因子,其OR为3.08（2.14~7.23）。结论：血清ICAM-1 有助于胰腺癌的早期诊断和预后评估。     

Serum trace element levels in patients with bladder cancer

Trace elements are essential components of biological structures, but they can be toxic at concentrations beyond those necessary for their biological functions. In the present study, groups of 35 patients with bladder cancer and 34 healthy volunteer controls were measured for trace elements using a furnace atomic absorption spectrophotometer. Serum levels of Cd, Ni and Co were increased (p<0.05) and Mn and Zn were decreased (p<0.05) in patients with bladder cancer. In the present study, a relationship was seen between the level of trace elements and the occurrence of bladder cancer, suggesting that an increase in the serum level of Cd, Ni, Co and a decrease in the levels of Zn and Mn might be important causes of bladder cancer occurrence; however, defining such a cause-and-effect relationship needs several prospective studies to be done, which seems necessary with regard to the high prevalence of this cancer.