Glycodelin与IL-17在子痫前期患者底蜕膜中的表达及相关性分析

目的:探讨glycodelin与IL-17在子痫前期患者底蜕膜中的表达,分析glycodelin与IL-17在子痫前期患者发病过程中在免疫抑制方面的相互作用。方法:选取子痫前期患者34例为实验组,正常足月妊娠患者30例为对照组,应用免疫组织化学法检测其底蜕膜中glycodelin及IL-17的表达情况。结果:1Glycodelin在34例子痫前期患者底蜕膜中的阳性表达率是14.17%;在30例对照组阳性表达率为16.67%。两组比较,在底蜕膜中glycodelin表达经统计学分析无差异(P>0.05)。2IL-17在34例子痫前期患者底蜕膜中的阳性表达率是79.41%;在30例对照组阳性表达率为13.33%。两组比较,在底蜕膜中IL-17表达有统计学差异(P<0.05),且随着病情加重IL-17表达逐渐增加。3Glycodelin与IL-17子痫前期患者底蜕膜中表达两者呈现负相关(r=-0.984,P<0.05)。结论:Glycodelin可能在妊娠过程中通过自身在底蜕膜中表达减少并上调IL-17在底蜕膜中的表达进一步过度激活免疫系统,进而导致子痫前期的发生发展。     

The Effect of Niceritrol (Pentaerythritoltetranicotinate) on Experimental Atherosclerosis in Cholesterol-fed Rabbits at Different Levels of Plasma Cholesterol

The action of the hypolipaemic drug niceritrol (INN) on cholesterol accumulation in the aorta was studied in cholesterol-fed rabbits at three levels of plasma cholesterol, approximately 100, 500–1000 and 2000 mg/100 ml. This was achieved by feeding diets with three different concentrations of cholesterol. The two higher but not the lowest concentration resulted in a large increase in plasma cholesterol as well as in a moderate lipid infiltration of the aorta. On addition of 0.5–1% niceritrol to the diet there was a significant reduction of plasma cholesterol in all three series. In the series with the lowest plasma cholesterol level the degree of atherosclerotic changes were so slight that the effect of niceritrol could not be evaluated. In the series with plasma cholesterol up to 1000 mg/100 ml niceritrol markedly inhibited the lipid infiltration of the aorta. In the series with the highest plasma cholesterol level niceritrol had no protective effect. The relationship between plasma cholesterol levels and extent of aortic lipid infiltration was studied in a pooled reference material of 154 cholesterol-fed rabbits. The demonstrated type of relationship could largely explain the difference in the protective action of niceritrol in the three series.     

膜叶槌果藤水提物镇痛抗炎作用的实验研究

目的：研究膜叶槌果藤水提物镇痛抗炎作用。方法：小鼠分别连续灌胃给予膜叶槌果藤水提物高、中、低剂量（10.88,5.44,2.72 g/kg）7 d,采用扭体法、福尔马林致痛模型、二甲苯致耳肿胀模型及腹腔毛细血管通透性实验,观察其镇痛抗炎作用。结果：膜叶槌果藤水提物可显著抑制冰醋酸引起的小鼠扭体反应次数（P＜0.01）;对福尔马林致小鼠疼痛舔足第Ⅰ,Ⅱ时相累积时间均有显著减少（P＜0.05或P＜0.01）;对二甲苯致小鼠耳肿胀有显著的抑制作用（P＜0.05或P＜0.01）;对冰醋酸引起的毛细血管通透性也有显著的抑制作用（P＜0.01）。结论：膜叶槌果藤水提物具有显著的镇痛抗炎作用。     

脂必妥对大鼠实验性高脂血症的作用

目的研究脂必妥对大鼠实验性高脂血症的调血脂作用。方法采用喂养法建立动物模型,给予不同剂量的脂必妥,4周后取血测定血脂含量。结果脂必妥可明显降低血清总胆固醇和甘油三酯的水平,显著提高血清高密度脂蛋白胆固醇。结论脂必妥对大鼠实验性高脂血症有明显的调血脂作用。     

IL-6在早孕绒毛蜕膜中的表达及米非司酮对其的影响

目的观察IL-6在早孕绒毛、蜕膜组织中的表达及米非司酮对其表达的影响,探讨药物流产的分子免疫作用机制。方法采用免疫组化染色测定早孕绒毛及蜕膜组织中IL-6的表达,其中正常早孕人工流产组20例,米非司酮药物流产组22例。结果IL-6在正常早孕绒毛及蜕膜组织中均呈阳性表达,米非司酮药物流产组表达强度明显降低(P<0.01)。结论IL-6在早孕绒毛及蜕膜中有较高水平表达,可能与胚泡着床、妊娠维持有关,米非司酮明显降低其表达,影响胚胎发育引起流产。     

原因不明复发性流产患者蜕膜和绒毛组织MMP-2与TIMP-2表达

目的检测原因不明复发性流产患者孕早期蜕膜和绒毛组织中基质金属蛋白酶-2(MMP-2)及其特异性组织抑制物-2(TIMP-2)的表达,探讨蜕膜在其发病中的作用。方法以免疫组化染色方法检测原因不明复发性流产患者25例蜕膜和绒毛组织中MMP-2、TIMP-2的表达。结果蜕膜组织中MMP-2表达(87±20)低于绒毛组织(98±30)(P<0.05);蜕膜组织MMP-2/TIMP-2(0.9±0.3)低于绒毛组织中(1.0±0.4)(P<0.05)。结论原因不明复发性流产患者母胎界面局部蜕膜的MMP-2表达显著减少,MMP-2/TIMP-2比值显著降低,其在原因不明复发性流产的发病中有重要的作用。     

Prevention of Carbon Tetrachloride-Induced Hepatotoxicity by the Ethanol Extract of Capparis moonii Fruits in Rats

The effect of the ethanol extract of Capparis moonii Hook. f. Thoms. (Capparidaceae) fruits was studied in carbon tetrachloride (CCl₄)-induced hepatotoxicity in rats. The hepatotoxicity was induced in rats with the administration of 1 : 1 (v/v) mixture of CCl₄ in olive oil at the dose of 1 ml/kg subcutaneously on day 7. The ethanol extract of C. moonii (200 mg/kg) and the standard drug silymarin (25 mg/kg) were given orally from day 1 to day 9. The extract of C. moonii produced significant (p &lt; 0.001) lowering of the elevated Serum glutamic oxaloacetic transaminace (SGOT) Serum glutamicpyraric transaminase (SGPT), alkaline phosphatase (ALP), and a rise of depleted total protein when compared with the toxic control. The results were comparable with the standard drug silymarin.     

山蒟提取物对兔动脉粥样硬化的影响

目的:观察山蒟提取物对高脂喂养家兔动脉粥样硬化的影响,并初步探讨其作用机制。方法:将家兔随机分为高脂组和山蒟(高、低剂量)组,高脂食物饲养3wk后给予药物或生理盐水,12wk周后考察并测定其血脂、主动脉病变程度、冠状动脉病变情况、血清总超氧化物歧化酶(SOD)和丙二醛(MDA)活性,并设对照组。结果:与高脂组比较,山蒟高剂量组血胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白(LDL)显著降低(P<0.05),高、低剂量组主动脉及冠状动脉病变程度显著降低(P<0.05),血清SOD活性升高,血清MDA活性降低(P<0.05)。结论:山蒟提取物能够明显降低兔动脉粥样硬化病理病变程度,具有良好的抗氧化能力,并且对血脂具有一定的调节作用。     

The effect of food on the pharmacokinetic properties and bioequivalence of two formulations of pitavastatin calcium in healthy Chinese male subjects

1.?Pitavastatin is an effective treatment for primary hyperlipidemia and mixed dyslipidemia. The aim of the present study was to investigate the effect of food on the pharmacokinetic properties and bioequivalence of the original, branded, formulation of pitavastatin calcium and a new generic formulation in healthy Chinese male subjects under fasting and fed conditions.

2.?Under fasting and fed conditions, 90% CIs of the geometric mean of generic/branded AUC_0–48?h ratios were 92.2–102.4%, 93.1–104.5%, the ratios of ln(AUC_0–∞) were 92.6–103.7%, 93.2–103.5%, and ln(C_max) ratios were 90.7–110.3%, 84.7–100.8%, respectively. The generic and branded formulations were bioequivalent in terms of rate and extent of absorption under both the conditions. The average values of AUC_0–48?h, AUC_0–∞ and C_max decreased noticeably following a high-fat breakfast. Values for AUC_0–48?h were 87.69% and 83.7%, values for AUC_0–∞ were 87.5% and 84.6%, and values for C_max were 45.0% and 50.4% in subjects given the generic and branded preparations, respectively. The absorption of pitavastatin calcium tablets was delayed following a high-fat meal, with T_max increasing by up to 2.43-fold.

3.?Both formulations were generally well tolerated, with no serious adverse reactions reported. The newly developed generic formulation may provide a reliable alternative to the branded tablets for patients with primary hyperlipidemia or mixed dyslipidemia.     

Hepatoprotective Effect of Houttuynia cordata Thunb Extract against Carbon Tetrachloride-induced Hepatic Damage in Mice

Houttuynia cordata Thunb (Saururaceae) is a traditional medicinal herb used to treat several disease symptoms. The present study was focused on the hepatoprotective effects of H. cordata ethyl acetate extract in experimental mice. Further the antioxidant potential of the extract was also evaluated to substantiate its hepatoprotective properties. Carbon tetrachloride-induced hepatic damage in mice was used to measure the serum biochemical parameters. Morphological changes in hepatocyte architecture were studied by haematoxylin and eosin staining. In vitro alkyl and hydroxyl free radical scavenging assays were performed to evaluate the antioxidant effect. Administration of H. cordata extract significantly reduced the elevated serum levels and regulated the altered levels of serum cholesterol in carbon tetrachloride-treated mice (P<0.05). The morphological changes in hepatocyte architecture were also reversed by H. cordata treatment. Further, the extract showed significant antioxidant actions by scavenging the alkyl and hydroxyl free radicals. The concentration of the extract necessary for 50% scavenging of alkyl and hydroxyl radicals was 15.5 and 410 μg/ml, respectively. H. cordata extract exhibited significant hepatoprotective property in carbon tetrachloride-induced hepatotoxicity in mice. The strong antioxidant activities possessed by the extract might be responsible for such actions.     

Radioprotective Property of Emblica officinalis. Fruit Ethanol Extract

Abstract

The radioprotective activity of Emblica officinalis. Gaertn. (Euphorbiaceae) ethanol extract (EOE) has been studied using rat liver mitochondria and pBR 322 plasmid DNA as two model in vitro. systems. EOE could effectively prevent γ-ray–induced lipid peroxidation as assessed by measuring thiobarbituric acid reactive substrates, lipid hydroperoxide, and conjugated diene. It also protected mitochondrial SOD against γ-ray–induced damage. Likewise, it prevented radiation-induced DNA strand breaks in a concentration-dependent manner. The radioprotective activity of EOE could be attributed to its hydroxyl and superoxide radicals scavenging property along with its lymphoproliferative activity. The radical scavenging capacity of EOE could be attributed to its constituent phenolics. The in vitro. radioprotective activity of EOE was significantly better compared with the in vivo. results reported earlier by others with the aqueous extract or fruit pulp of E. officinalis.. The higher concentrations of the more bioavailable phenolics such as gallic acid and ellagic acid in EOE compared with the other preparations might possibly account for the results.     

蒙药童格勒格-1对大鼠高脂血症的影响

目的观察蒙药童格勒格-1(TGLG-1)对高脂血症大鼠血清血脂的调节作用。方法建立大鼠高血脂模型。高血脂症大鼠分为四组,一组为实验对照组,其余三组分别以低、中、高剂量TGLG-1灌胃,21d后测定血脂值。结果与高脂对照组相比,中、高剂量TGLG-1能显著降低血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白一胆固醇(LDL-C)水平。结论TGLG-1具有良好的降低血脂浓度和预防高血脂症的作用。     

牡丹皮水提取物及乙醇提取物的抗菌作用研究

目的研究牡丹皮水提取物及不同浓度乙醇提取物的体外抑菌和杀菌作用。方法用试管内药液稀释法测定牡丹皮水提取物及不同浓度乙醇提取物的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。结果牡丹皮水提取物和乙醇提取物对金黄色葡萄糖球菌、大肠杆菌及绿脓杆菌均有较强的抑制作用和一定的杀灭作用,且水提取物的作用强于乙醇提物。结论牡丹皮具有抗菌作用。     

Antifertility Effects of Sapindus trifoliatus L. Fruit Extract in Rats

Abstract

A saponin rich fraction was isolated from the fruit pulp of Sapindus trifoliatus L. It was tested for postcoital antifertility effects in female albino rats on different days of pregnancy. When injected intraperitioneally (20 mg/rat) on days 6–9 or on days 1–14, it impaired fertility in 7/10 and 10/10 animals, respectively. However, no significant fertility impairment occurred when this dose was administered on Days 1–3, 1–7 or 4–7. This fraction also appeared to be antiestrogenic when tested with 1 μg 170-estradiol.     

东莨菪碱对兔肝性脑病模型的保护作用研究

目的:研究东莨菪碱对兔肝性脑病模型的保护作用。方法:采用肝叶大部分切除术联合十二指肠注入复方氯化铵溶液的方法复制肝性脑病模型兔28只,于第2次注入复方氨化铵后分为4组,即空白对照组(5%葡萄糖注射液),东莨菪碱组(0.015mg·kg-1)、复方谷氨酸钠组(0.5g·kg-1)及合用组(2药同剂量),进行一次性耳缘静脉给药后,观察各组兔给药后抽搐情况、存活时间和给药前与给药10min后的血氨水平变化。结果:与空白对照组比较,东莨菪碱组、复方谷氨酸钠组和合用组兔抽搐潜伏期和存活时间延长、抽搐持续时间缩短、血氨降低(P<0.05或P<0.01),其中合用组保护作用最显著。结论:东莨菪碱与复方谷氨酸钠合用对肝性脑病具有协同保护作用。     

复方螺旋藻片对高血脂症小鼠血脂的影响

目的：观察复方螺旋藻片（FST）对高脂血症小鼠血脂的影响。方法：用FST1.5mg/kg,3g/kg分别灌胃给予高脂血症小鼠,连续14d后,眼眶取血测血脂。结果：与高脂血症模型对照组相比,FST高、低剂量能显著降低血清TC、TG和LDL－C水平,明显升高血清HDL－C水平与HDL－C／TC比值。同时FST还能升高血清apoAI并降低apoB(但FST 1.5g/kg对apoB无明显影响）。结论：FST能明显降低高脂血症小鼠血脂水平,并对载脂蛋白有一定的调节作用。     

匹伐他汀治疗原发性高胆固醇血症的疗效与安全性评价

目的 评价匹伐他汀治疗原发性高胆固醇血症的疗效和安全性.方法 随机、单盲(对研究者设盲)、阳性药物平行对照.46例原发性高胆固醇血症患者随机分为匹伐他汀(2和4 mg)组和阿托伐他汀(10 mg)组,研究为期8周,观察治疗前后患者血脂[总胆固醇(TC)、高密度脂蛋白-胆固醇(HDL-C)、低密度脂蛋白-胆固醇(LDL-C)、甘油三酯(TG)]、丙氨酸氨基转移酶/天冬氨酸氨基转移酶(ALT/AST)及磷酸肌酸激酶(CPK)水平的变化.结果 治疗4和8周各组TC、LDL-C水平均明显降低(P<0.01), 且4周与8周无显著差异;治疗8周各组LDL-C达标率分别为62.5%、66.7%和86.7%;但3组HDL-C和TG水平治疗前后无明显变化.匹伐他汀(2 mg)组1例出现CPK水平异常升高,停药2周后恢复正常.结论 匹伐他汀能有效降低原发性高胆固醇血症患者的TC和LDL-C水平,疗效与阿托伐他汀相似,且基本安全.     

红山胶囊对试验性高脂血症大鼠的治疗作用

目的 观察红山胶囊的调脂效果.方法 取72只SD雄性大鼠,给予普通饲料预饲1周取血,检测血清总胆固醇(TC).根据血清TC水平随机分为普饲组、模型组、关降之组及红山胶囊高、中、低剂量组,每组12只大鼠.试验开始时,除普饲组外,其余各组挟用高脂饲料造模.造模后第11天各组分别给予0.5%羧甲基纤维素钠,关降之4mg/kg,红山胶囊4,2,1 g原生药/kg.每日灌胃1次,给药后第28天晚禁食不禁水,次日晨取血检测甘油三酯(TG)、TC、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C),并计算TC/HDL-C.结果 红山胶囊2,4 g原生药/kg剂量对大鼠试验性高脂血症有明显的治疗作用,能呈刺量依赖性降低血清TC,TG,LDL-C.TC/HDL-C,对血清HDL-C则有升高或抑制其降低作用.红山胶囊1 g原生药/kg对大鼠试验性高脂血症模型也有一定的调脂作用.结论 红山胶囊对大鼠试验性高脂血症有显著的治疗作用.     

RP HPLC测定家兔血浆中银杏叶提取物浓度方法研究

目的:建立用反相高效液相色谱(RP HPLC)测定家兔血浆中银杏叶提取物(extract ginkgo biloba, EGB)浓度的方法,对银杏叶提取物在家兔体内的药物动力学进行研究.方法:采用ODS Hypersil色谱柱(100 mm×2.1 mm,5 μm)柱温22℃,甲醇 水 磷酸为流动相(32∶67.6∶0.4),流速1.0 mL&#8226;min 1,紫外检测波长368 nm等条件下进行分析测定.结果:最低检测浓度1.25 μg&#8226;mL 1,方法平均回收率分别为98.82%和101.06%精密度为107.80%,相对标准差(RSD)＜4%(n＝3),线性范围2.50～5.00 μg&#8226;mL 1(r＝0.997 2,n＝5)和6.25～15.00 μg&#8226;mL 1(r＝0.995 7,n＝5).结论:该法具有操作简单、干扰小、准确灵敏等优点.为临床应用EGB提供实验依据.     

Effect of an Aqueous Extract of Salsola collina on the Course of Experimental Cholelithiasis in Rabbits

Pharmaceutical Chemistry Journal -