秋水仙碱抗肝纤维化疗效观察

目的　研究秋水仙碱抗肝纤维化作用。方法　 49例慢性乙型肝炎和 35例肝炎后肝硬化患者随机分为秋水仙碱治疗组 (46例 )和对照组 (38例 ) ,治疗前后放免法测定血清肝纤维化指标 :IV型胶原、透明质酸、和层粘蛋白。结果秋水仙碱治疗后血清IV型胶原、透明质酸和层粘蛋白显著降低 (P 0 0 1 ) ,对照组三项指标变化不明显 (P 0 0 5 )。结论　秋水仙碱具有抗肝纤维化作用 ,可用于慢性乙型肝炎及肝炎后肝硬化的抗肝纤维化治疗。     

秋水仙碱抗肝纤维化疗观察

目的 研究秋水仙碱抗肝纤维化作用。方法 ４９例慢性乙型肝炎和３５例肝炎后肝硬化患者随机分为秋水仙碱治疗组（４６例）和对照组（３８例）,治疗前后放免法测定血清肝纤维化指标：ＩＶ型胶原、透明质酸、和层粘蛋白。结果 秋水仙碱治疗后血清ＩＶ型胶原、透明质酸和层粘蛋白显著降低（Ｐ〈０．０１）,对照组三项指标变化不明显（Ｐ〉０．０５）。结论 秋水仙碱具有抗肝纤维化作用,可用于慢性乙型肝炎及肝炎后肝硬化的抗肝纤     

风湿1号颗粒的抗痛风作用

目的：研究风湿1号颗粒的抗痛风作用。方法：风湿1号颗粒ig给药,并用消炎痛和秋水仙碱作对照,结果：在5,10,20g生药剂量下,具有非常显著的抗痛风性关节 炎的作用,其中对尿酸钠诱导的小鼠足肿,大鼠足肿对家兔急性痛风性关节炎的抑制作用较强,在以上几种实验性痛风性关节炎模型上,该药的抗痛风作用强于阳性对照药消炎痛和秋水仙碱,结论：风湿1号颗粒在临床上通史治疗痛风性关节炎。     

雷公藤的抗炎抗免疫作用研究进展

雷公藤是目前临床使用较多的中药免疫抑制剂,其具有抗炎、抗免疫、抗增殖、促凋亡等作用,在多种自身免疫性和炎症性疾病中有广泛应用。雷公藤及其提取物发挥抗炎抗免疫作用的过程十分复杂,主要涉及免疫细胞(T淋巴细胞、B淋巴细胞、树突状细胞、单核细胞、巨噬细胞等)、炎症介质(肿瘤坏死因子-α、白细胞介素-6、白细胞介素-1β等)、酶类等,具体机制有待进一步研究。因此,探索雷公藤的抗炎抗免疫作用机制具有重要意义。相信随着研究的不断深入,未来雷公藤的抗炎抗免疫作用将会得到更深的认识,其将用于更多疾病的治疗。     

抗幽门螺杆菌感染治疗进展

幽门螺杆菌与慢性胃炎、消化性溃疡、胃癌及MALT淋巴瘤等疾病的发生发展关系密切，因此，抗幽门螺杆菌感染对这些疾病的防治具有重要的作用。本文明确了国内外抗幽门螺杆菌感染治疗的临床指征、根除指征、根除方案以及根除幽门螺杆菌感染失败者的补救方案，从而使抗幽门螺杆菌感染的治疗更加简捷化。     

抗痛风药的作用机制与分类

抗痛风药根据其作用方式可分为五类 :①抑制粒细胞浸润药 ,如秋水仙碱 ;②抑制尿酸生成药 ,如别嘌呤醇 ;③促进尿酸排泄药 ,如丙磺舒、磺吡酮、苯溴马隆等 ;④非甾体抗炎药 ,如吲哚美辛、保泰松、炎痛喜康、萘普生、布洛芬等 ;⑤糖皮质激素类 ,如强的松。治疗痛风急性发作 ,可应用秋水仙碱、非甾体抗炎药和糖皮质激素 ;控制慢性痛风可用促尿酸排泄药和抑制尿酸生成药。1　抑制粒细胞浸润药秋水仙碱 (ｃｏｌｃｈｉｃｉｎｅ) :作用及作用机制 :本品为治疗痛风急性发作的特效药 ,对痛风的急性发作有选择性抗炎作用 ,一般于服药后 6～ 12小时关…     

以肺栓塞为主要表现的抗磷脂综合征1例报告

抗磷脂综合征，是指机体内抗磷脂抗体增高引起的一组临床疾病的总称。临床表现有反复血栓形成、习惯性流产、血小板减少、肾脏系统损害及神经、精神症状等[1]。本组疾病发病率、认知率较低，且在大部分医院无相应检测手段，早期诊断存在一定难度。现报告1例抗磷脂综合征患者，以肺栓塞为主要表现，伴有血液、肾脏、呼吸等多系统损害，结合文献资料复习，以加深对本组疾病的认识。     

抗中性粒细胞胞浆抗体的临床应用

抗中性粒细胞抗体（ANCA）是一种针对中性粒细胞和单核细胞胞浆成分的自身抗体。在临床上与一些疾病密切相关，可作为诊断系统性血管炎等相关疾病的血清学标志物。对于自身免疫疾病，肾小球肾炎，炎性肠病等均有临床意义。经过不断的研究ANCA对临床疾病的诊断和鉴别价值将更广泛。     

浅谈抗微生物药物的不合理应用

抗微生物药物是目前临床应用较广，用量较大的一类药物，在控制与治疗疾病方面发挥了重大的作用。随着科学技术的不断发展，抗微生物药物新品种不断增多，在临床上存在着不合理应用和滥用的问题也越来越严重。笔者对抗微生物药物的不合理应用现状及对策，谈以下看法。     

抗血小板药物的研究进展

血小板聚集可以导致血栓形成、动脉粥样硬化等心脑血管疾病，而抗血小板药物预防血栓生成的作用非常明显。现简要介绍近年来抗血小板药的研究概况，包括阿司匹林、噻氯吡啶、双嘧达莫、氯吡格雷、拉米非班等。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.