全自动血细胞分析仪联合血涂片细胞形态学在血常规检验中的应用意义

目的 探究在血常规检验中应用全自动血细胞分析仪+血涂片细胞形态学的意义。方法 选取200例行血常规检验的受检者作为研究对象,依次行全自动血细胞分析仪、血涂片细胞形态学检验,以复检结果作为金标准,比较三种检验方法的诊断效能及相关细胞的阳性检出情况。结果 以复检结果作为金标准,结果显示,阳性182例,阴性18例。全自动血细胞分析仪检验诊断符合率为81.50%、灵敏度为83.52%、特异度为61.11%;血涂片细胞形态学检验诊断符合率为85.50%、灵敏度为87.36%、特异度为66.67%;联合检验诊断符合率为96.50%、灵敏度为96.70%、特异度为94.44%。联合检验诊断符合率、灵敏度、特异度均显著高于全自动血细胞分析仪检验和血涂片细胞形态学检验,差异具有统计学意义(P<0.05)。全自动血细胞分析仪检验和血涂片细胞形态学检验对红细胞、血红蛋白、单核细胞、白细胞、淋巴细胞、嗜酸性细胞、中性粒细胞、嗜碱性细胞的阳性检出率比较差异均无统计学意义(P>0.05);联合检验对红细胞、血红蛋白、单核细胞、白细胞、淋巴细胞、嗜酸性细胞、中性粒细胞、嗜碱性细胞的阳性检出率均显著高于全...     

全自动血细胞分析仪与血涂片细胞形态学在血常规检验中的应用价值

目的 研究全自动血细胞分析仪与血涂片细胞形态学在血常规检验中的应用价值。方法 选取福建省周宁县医院收治的血常规检验患者116例为研究对象,时间为2021年8月至2022年10月,按照检验方法分为对照组（全自动血细胞分析仪）和观察组（血涂片细胞形态学）,以复检结果为金标准,对比两组血常规检验患者在全自动血细胞分析仪与血涂片细胞形态学检验下的血常规指标阳性情况、血常规检验结果、相关细胞阳性检出情况、相关细胞阳性检出情况、诊断效能。结果 血常规检验患者在血涂片细胞形态学检验下白细胞计数（WBC）、降钙素原（PLT）、红细胞计数（RBC）各项血常规指标阳性检出率高于全自动血细胞分析仪检验（P <0.05）。血常规检验患者在血涂片细胞形态学检验下白细胞、红细胞、血红蛋白、淋巴细胞、单核细胞、中性粒细胞、嗜酸性细胞、嗜碱性细胞相关细胞阳性检出率高于全自动血细胞分析仪检验（P <0.05）。血常规检验患者在血涂片细胞形态学检验下有核红细胞、异型淋巴细胞、中毒颗粒/空泡变性部分项阳性检出率高于全自动血细胞分析仪检验（P <0.05）。血常规检验患者在血涂片细胞形态学检验下敏感度与全自...     

全自动血细胞分析仪与血涂片细胞形态学在血常规检验中的应用效果分析

目的探究在血常规检验中应用全自动血细胞分析仪与血涂片细胞形态学的临床效果。方法在2017年11月至2018年12月,对40例血常规检验患者做出研究,并且对每个患者实现静脉血样采集。其中,主要利用全自动血细胞分析仪与血涂片细胞形态学来检查,对比两个方式下的检查效果。结果通过全自动血细胞分析仪与血涂片细胞形态学的检查,发现在淋巴细胞、单核细胞、中性粒细胞方面的差异不显著,表现为P>0.05。在嗜碱性粒细胞检查中,其存在的差异化显著,符合P<0.05的差异性。结论在对患者进行血常规检验工作中,使用全自动血细胞分析仪与血涂片细胞形态学的应用效果不同,虽然全自动血细胞分析仪的应用价值较高,但存在的误差率较低。所以,加强全自动血细胞分析仪与血涂片细胞形态学的结合应用,将保证细胞阳性检出率的提升,其存在的应用价值也十分显著。     

血常规检验中应用全自动血细胞分析仪的价值

目的观察并探究在血常规检验中应用全自动血细胞分析仪的临床价值。方法选取186例来我院接受血常规检验的患者为研究对象,其血常规标本经血细胞分析仪测定,均与血细胞形态学的复检标准相符。随后再次采用全自动血细胞分析仪进行检测,并展开涂片镜检检测,对比并评价两种检测结果。结果在NE(中性粒细胞)、EO(嗜酸粒细胞)、BA(嗜碱粒细胞)以及LY(淋巴细胞)检测阳性率方面,两种检测方法呈现出较好的一致性;在IG(幼稚细胞/核左移)、MO(单核细胞)以及OTHER(原始细胞/异形淋巴细胞)分类检测阳性率方面,全自动血细胞分析仪的检测结果要稍低于涂片镜检,但两组数据差异不大,P> 0.05,不具备统计学意义。结论通过采用全自动血细胞分析仪进行检验,可为临床诊治提供准确的血细胞数量等参数,必要时通过与涂片显微镜镜检相结合,可充分促进临床血常规检验准确率及质量的提高,值得重视。     

全自动血细胞分析仪联合血涂片细胞形态学在血常规检验中的应用效果

目的研究全自动血细胞分析仪联合血涂片细胞形态学在血常规检验中的应用效果。方法选取进行血常规检验的10000例患者的10000份血液样本作为研究对象,对所有样本进行全自动血细胞分析仪检验及血涂片细胞形态学检验。统计所有样本中最终确定的阳性样本数,比较全自动血细胞分析仪和血涂片细胞形态学的阳性检出率。比较两种检验方法单独检验及联合检验的敏感性、特异性、准确度。结果10000份样本中最终确定阳性样本9395份。全自动血细胞分析仪检验出阳性9177份,阳性检出率为91.77%;血涂片细胞形态学检验出阳性9207份,阳性检出率为92.07%。两种检验方法的阳性检出率比较,差异无统计学意义(χ²=0.606,P=0.436>0.05)。血涂片细胞形态学检验的敏感性为96.00%(9019/9395),特异性为68.93%(417/605),准确度为94.36%(9436/10000);全自动血细胞分析仪检验的敏感性为95.36%(8959/9395),特异性为63.97%(387/605),准确度为93.46%(9346/10000);联合检验的敏感性为100.00%(9395/9395),特异性为100.00%(605/605),准确度为100.00%(10000/10000)。联合检验的敏感性、特异性、准确度均高于单独检验,差异具有统计学意义(P<0.05)。结论临床在进行血常规检验的过程中,单纯应用全自动细胞分析仪和单纯应用血液涂片形态学检验都存在着各自的优点和缺点,会导致在临床检验过程中存在一定的假阳性或假阴性情况,联合两种检验手段进行血常规检查,能提升整体的检验效率和精确度。     

探究全自动血细胞分析仪在血常规检验中的应用价值

目的 探究全自动血细胞分析仪在血常规检验中的应用价值.方法 回顾性分析1712份血常规标本资料,经血细胞全自动血细胞分析仪检测并符合血细胞形态学复检标准的共878份,均行全自动血细胞分析仪和涂片镜检测,比较两种检验方法的血常规检验结果.结果 全自动血细胞分析仪对中性粒细胞(NE)、嗜酸粒细胞(EO)、嗜碱粒细胞(BA)...     

全自动血细胞分析仪与血涂片在血常规中的应用

目的研究全自动血细胞分析仪和血涂片细胞形态学在血常规检验中的应用效果。方法纳入100例患者作为此次研究的对象,100例患者均在我院接受血常规检验,并自愿参与此次研究,纳入时间段为2017年12月至2018年12月期间,在随机抽签分组的基础上,根据对患者实施的血常规检验的方法的不同,将患者均分为对照组(n=50)和观察组(n=50)。对照组以血涂片细胞形态学的方法进行血常规检验,观察组以全自动血细胞分析仪和血涂片细胞形态学联合检验的方法进行血常规检验,对比其检验结果的阳性率。结果观察组血常规检验结果为阳性的有3例,阳性率为6%,对照组血常规检验结果为阳性的有11例,阳性率为22%,组间差异显著(P <0.05)。结论对于血常规检验的方法选择来说,血涂片细胞形态学和全自动血细胞分析仪联合检验的方法有着更好的效果,检验结果的阳性率也更佳,因此,值得在临床上进行推广及应用。     

血涂片分析在血常规检验中的运用与结果探究

目的探究在血常规检验中实施血涂片的结果和价值。方法 128例开展血常规检验患者,以检验方法不同为依据分为实验组与对照组,各64例。对照组的检验方法为血常规常规检验,实验组则实施血涂片分析检验。比较两组检验结果、异常细胞检出率,分析实验组异常血液样本。结果实验组异常检出率45.31%高于对照组的28.13%,假阴性率1.56%、假阳性率0,均低于对照组的10.94%、6.25%,差异有统计学意义(P<0.05)。实验组异常嗜碱性细胞检出率高于对照组,差异有统计学意义(P<0.05);两组异常嗜酸性细胞、异常淋巴细胞、异常单核细胞、异常中性粒细胞检出率比较,差异无统计学意义(P>0.05)。实验组异常血液样本29例, 11例患者核红细胞、幼稚细胞出现;5例患者中心淡染区扩大、红细胞呈现大小不均情况;4例患者呈现中毒颗粒少量、中性粒细胞或白细胞提升情况;9例患者呈现空泡、核左移(程度不同)。结论在血常规检验中运用血涂片分析可获得满意的检验目标,可对细胞形态进行明确,使得异常检出率提升,建议推广。     

血液细胞形态学检查在血常规检测中的应用价值分析

目的分析血液细胞形态学检查在血常规检测中的应用价值。方法将我院2016年5月～2018年12月接受血常规检验的180例标本纳入到本研究中,按照随机数字表法分为对照组(90例,接受全自动血细胞分析仪检测)和研究组(90例,接受全自动血细胞分析仪联合血液细胞形态学检查),比较检测结果。结果研究组RDW-SD、HCT、MCV、MCHC、MCH与对照组比较,差异无统计学意义(P 0.05)。研究组RDW-SD、HCT、MCV、MCHC、MCH异常检出率均高于对照组,差异有统计学意义(P 0.05)。结论临床在做血常规检测时,采用全自动血细胞分析仪联合血液细胞形态学进行检查,可以有效检验血常规指标水平,且对相关疾病的鉴别能力更强,应用前景广。     

ADVIA2120血液分析仪对动物血细胞的分析性能评价

目的对ADVIA2120血细胞分析仪的动物血细胞分析性能进行评价。方法对该仪器的准确度、精密度、线性和携带污染率进行评价,并与Hemavet 950FS动物血细胞分析仪测定结果及人工白细胞分类镜检结果进行比较。结果该仪器的准确度、批内和批间精密度、携带污染率和线性均在允许范围内;与Hemavet 950FS动物血细胞分析仪测定结果相关性白细胞(WBC)、红细胞(RBC)、血红蛋白(HGB)、血小板(PLT)较好,红细胞压积(HCT)较差;白细胞分类测定结果与人工显微镜分类比较,中性粒细胞、淋巴细胞、嗜酸粒细胞相关性较好,单核细胞、嗜碱粒细胞相关性较差。结论 ADVIA2120全自动血细胞分析仪在测定动物全血样本时准确度、精密度、线性较好,携带污染率低,有较强的筛选能力,是一台性能良好的全自动血细胞分析仪,可以满足药物安全性评价课题动物血液样本分析的需要。     

造血干细胞移植

20世纪50年代初期造血干细胞移植兴起,随着医学科技发展,大剂量化疗(预处理)+造血干细胞移植为各种晚期肿瘤和癌症治疗提供了有力的支持,目前对造血干细胞的来源、种类、采集、活性测定,异基因造血干细胞的配型、动员剂的效果、骨髓中癌细胞污染的处理、移植后并发症的诊治、疾病观察指标、临床适应证和治疗效果的评估等等都逐渐规范化,且有很多治疗成功病例的报告。     

血细胞仪器分析后血涂片复审准则制定与应用

目的 拟制定适合本实验室血细胞仪器分析后血涂片复审准则,以保证血细胞分析结果的准确性和可靠性,更有利于临床对疾病的诊断和治疗.方法 在雅培CELL-DYN3700五分类血细胞计数仪上检测1486份静脉血,先以“国际血涂片复审准则”为标准,记录仪器检测所得各项数据、报警类别和显微镜下所见.结合本实验室实际情况和仪器性能特点,制定适合的血涂片复审准则,并以200份标本验证.结果 以“41条规则”复审,1486份标本真阴性60.4％、真阳性20.8％、假阴性3.0％、假阳性15.8％.以本实验室血涂片复审准则验证200份标本,真阴性69.0％、真阳性18.5％、假阴性2.5％、假阳性10.0％,具有诊断意义的重要参数（幼稚细胞）无漏检.复检率由于36.6％下降到达28.5％.结论 血细胞仪器分析具有局限性,对于形态结构异常的细胞不能作出准确的判断和定位,实验室应根据自身实际情况制定合适的血涂片复审准则,以保证血细胞分析结果的准确性和可靠性.     

A semi-mechanistic red blood cell survival model provides some insight into red blood cell destruction mechanisms

Most mathematical models developed for the survival of haematological cell populations, in particular red blood cells (RBCs), follow the principle of parsimony. They focus on the predominant destruction mechanism of age-related cell death (senescence) and do not account for within subject variability in the RBC lifespan. However, assessment of the underlying physiological destruction mechanisms can be of interest in pathological conditions that affect RBC survival, for example sickle cell anaemia or anaemia of chronic kidney disease. We have previously proposed a semi-mechanistic RBC survival model which accounts for four different types of RBC destruction mechanisms. In this work, it is shown that the proposed model in combination with informative RBC survival data is able to provide a deeper insight into RBC destruction mechanisms. The proposed model was applied in a non-linear mixed effect modelling framework to biotin derived RBC survival data available from literature. Three mechanisms were estimable based on the available data of twelve subjects, including random destruction, senescence and destruction due to delayed failure. It was possible to identify three subjects with a decreased RBC survival in the study population. These three subjects all showed differences in the contribution of the estimated destruction mechanisms: an increased random destruction, versus an accelerated senescence, versus a combination of both.     

Modeling red blood cell survival data

Anaemia of chronic kidney disease (CKD) is a common complication in patients with renal impairment, especially in end-stage renal failure. As well as erythropoietin deficiency, decreased red blood cell survival is a contributing factor. However, it remains unclear which mechanism underlies the altered survival of red blood cells (RBCs). In this work a previously developed statistical model for RBC survival was applied to clinical data obtained from 14 patients with CKD undergoing hemodialysis as well as 14 healthy controls using radioactive chromium (⁵¹Cr) as random labelling method. A classical two-stage approach and a full population analysis were applied to estimate the key parameters controlling random destruction and senescence in the model. Estimating random destruction was preferred over estimating an accelerated senescence in both approaches and both groups as it provided the better fit to the data. Due to significant nonspecific random loss of the label from the cells that cannot be quantified directly only a relative RBC survival can be obtained from data using ⁵¹Cr as labelling method. Nevertheless, RBC survival was found to be significantly reduced in CKD patients compared to the controls with a relative reduction of 20–30% depending on the analysis method used.     

血库型白细胞过滤器在临床输血中的应用观察

目的观察国产血库型白细胞过滤器在临床输血中的应用效果。方法取保存期在6～10d的红细胞悬液进行白细胞过滤,检测过滤前后红细胞、白细胞计数,观察白细胞过滤和未过滤各300例患者中的非溶血性发热性输血反应(FNHTR)发生情况。结果红细胞悬液经过滤后,残余白细胞平均为4.2×106/L,白细胞清除率平均为99.95%,过滤前后白细胞含量有显著性差异(t=15.41,P<0.01),红细胞回收率平均为92.71%;红细胞悬液未过滤时临床发生FNHTR8.67%(26/300),白细胞过滤后,FNHTR降至0.67%(2/300);滤前及滤后1～6天内,红细胞悬液的上清游离血红蛋白和红细胞LDH差异无显著性变化。结论国产一次性白细胞过滤器可有效预防FNHTR的发生。     

Effects of red blood cell concentrate transfusion on blood tacrolimus concentration

Background Elevated blood concentration of tacrolimus is frequently observed following transfusion of red blood cell concentrate in patients after allogeneic hematopoietic stem cell transplantation. Objective The aim of this retrospective study was to clarify the effects of transfusion of red blood cell concentrate on the blood concentration of tacrolimus. Setting Chiba University Hospital in Japan. Method Fifty-two patients (aged 0–65 years) receiving both tacrolimus and transfusion after allogeneic hematopoietic stem cell transplantation were enrolled. The ratio of measurement after transfusion to measurement before transfusion was calculated for hematocrit and blood concentration/dose ratio of tacrolimus (termed the hematocrit ratio and the tacrolimus ratio, respectively). Main outcome measure Change in blood concentration/dose ratio of tacrolimus and variable factors associated with variation in tacrolimus ratio. Results The blood concentration/dose ratio of tacrolimus was increased after transfusion compared with before transfusion (p?<?0.001). A statistically significant correlation was seen between the hematocrit ratio and tacrolimus ratio (r?=?0.32, p?<?0.001). Hematocrit ratio, age or body surface area, and difference in aspartate aminotransferase level before and after transfusion were associated with the variation in tacrolimus ratio. There was no correlation between tacrolimus ratio and change in serum creatinine or potassium level in the short term. Conclusion Change in the blood concentration/dose ratio of tacrolimus was associated with change in the hematocrit ratio after transfusion, and more attention is required for children or patients with small body surface area. Dose adjustment of tacrolimus is required if the blood concentration of tacrolimus is much higher than the target concentration.

     

In-vitro red blood cell partitioning of doxycycline

Objective:

In-vitro red blood cell (RBC) partitioning of doxycycline was studied to determine whether doxycycline penetrates RBC and its concentration was assayed keeping in view its high lipophilicity.

Materials and Methods:

Standardization of doxycycline was performed in whole blood and plasma of cattle by microbiological assay using Bacillus subtillis ATCC 6633 as indicator organizm. Actual concentration of the drug was obtained by comparing zone inhibition with standard graph and the extent of partitioning was mathematically calculated.

Results:

The R² value of standard graph for doxycycline was 0.9934 and 0.9727 for plasma and whole blood, respectively. Overall, RBC partitioning of doxycycline was found to be 18.40 ± 1.70%.

Conclusions:

Overall RBC partitioning of doxycycline indicated low penetration into RBC. Plasma is the fluid suggested for pharmacokinetic evaluation of doxycycline.     

Models for the red blood cell lifespan

The lifespan of red blood cells (RBCs) plays an important role in the study and interpretation of various clinical conditions. Yet, confusion about the meanings of fundamental terms related to cell survival and their quantification still exists in the literature. To address these issues, we started from a compartmental model of RBC populations based on an arbitrary full lifespan distribution, carefully defined the residual lifespan, current age, and excess lifespan of the RBC population, and then derived the distributions of these parameters. For a set of residual survival data from biotin-labeled RBCs, we fit models based on Weibull, gamma, and lognormal distributions, using nonlinear mixed effects modeling and parametric bootstrapping. From the estimated Weibull, gamma, and lognormal parameters we computed the respective population mean full lifespans (95 % confidence interval): 115.60 (109.17–121.66), 116.71 (110.81–122.51), and 116.79 (111.23–122.75) days together with the standard deviations of the full lifespans: 24.77 (20.82–28.81), 24.30 (20.53–28.33), and 24.19 (20.43–27.73). We then estimated the 95th percentiles of the lifespan distributions (a surrogate for the maximum lifespan): 153.95 (150.02–158.36), 159.51 (155.09–164.00), and 160.40 (156.00–165.58) days, the mean current ages (or the mean residual lifespans): 60.45 (58.18–62.85), 60.82 (58.77–63.33), and 57.26 (54.33–60.61) days, and the residual half-lives: 57.97 (54.96–60.90), 58.36 (55.45–61.26), and 58.40 (55.62–61.37) days, for the Weibull, gamma, and lognormal models respectively. Corresponding estimates were obtained for the individual subjects. The three models provide equally excellent goodness-of-fit, reliable estimation, and physiologically plausible values of the directly interpretable RBC survival parameters.     

血细胞分析前质量控制分析

目的探讨血细胞分析前质量控制的重要性。方法通过近几年临床实验室开展血细胞分析前的质量控制评价活动,分析质量控制分析前的影响因素,明确血细胞分析前质量控制的工作要求。结果血细胞分析前质量控制的影响因素：检验科工作人员素质及仪器设备状态;分析前项目的正确选择;标本采集前患者的准备;正确的标本采集;标本的正确处理;药物及生理因素对检验结果的影响。结论血细胞分析前的质量保证,既能减少实验误差,还能提高血细胞检验质量。