长链非编码RNA HOTAIR在接受调强放射治疗鼻咽癌患者中的表达及其临床意义

目的:探讨长链非编码RNA (lncRNA)HOX转录本反义RNA(HOTAIR)在接受调强放射治疗鼻咽癌中的表达及其临床意义。方法:收集20例鼻咽癌患者肿瘤组织及对应癌旁组织的新鲜标本,279例经病理学证实接受调强放射治疗的鼻咽癌患者的组织标本、临床病理特征及随访资料。应用RT-qPCR法检测鼻咽癌组织中HOTAIR的表达情况,并对临床病理资料和随访资料进行统计分析。结果:20例鼻咽癌及其配对的正常鼻咽黏膜上皮组织中,75%(15/20)鼻咽癌组织lncRNA HOTAIR的表达水平明显高于正常鼻咽组织(P0.01);在279例鼻咽癌患者组织中,有59.9%(167/279)鼻咽癌组织HOTAIR表达升高; HOTAIR的高表达与T和N分期密切相关(P0.05),但与患者的年龄、性别、病理类型和M分期没有明显相关性;与lncRNA HOTAIR低表达组相比,HOTAIR高表达组的鼻咽癌患者疾病无进展生存率和总生存率明显下降(P0.05)。结论:lncRNA HOTAIR在鼻咽癌中表达水平升高,且与鼻咽癌患者的不良预后明显相关。     

Calreticulin通过诱导细胞EMT促进鼻咽癌迁移和侵袭

目的:观察钙网蛋白(calreticulin,CRT)在鼻咽癌组织中的表达并分析其临床病理学意义,揭示其对鼻咽癌CNE2细胞上皮-间充质转化(EMT)的影响。方法:S-P免疫组化法检测CRT在52例鼻咽癌组织和57例鼻咽部良性病变组织中的表达情况,并分析其临床病理学意义;构建特异性干扰CRT基因的小干扰RNA干扰载体,瞬时转染鼻咽癌CNE2细胞,观察CRT低表达对CNE2细胞形态的影响;Transwell迁移和侵袭实验检测CRT低表达对CNE2细胞迁移和侵袭能力的影响;Western blot检测CRT低表达对上皮型钙黏蛋白(E-cadherin)、波形蛋白(vimentin)、转化生长因子-β(TGF-β)及基质金属蛋白酶9(MMP-9)等EMT相关蛋白表达的影响。结果:CRT在鼻咽癌组织中的阳性表达率为82.69%(43/52),在鼻咽部良性病变组织的阳性表达率为19.29%(11/57),CRT在鼻咽癌组织中表达显著增高(P0.05);CRT的阳性表达率与鼻咽癌分期及淋巴结转移呈正相关(P0.05)。敲降CRT呈低表达后,CNE2细胞由梭形演变为扁平和鹅卵石样,且细胞排列紧密,细胞的迁移侵袭能力减弱(P0.05)。敲降CRT呈低表达后,E-cadherin表达显著增高,vimentin、TGF-β以及MMP-9蛋白的表达降低(P0.05)。结论:Calreticulin在鼻咽癌组织中表达显著增高,且与鼻咽癌临床分期及淋巴结转移呈正相关。Calreticulin可诱导鼻咽癌CNE2细胞发生EMT,进而促进鼻咽癌的迁移和侵袭。     

鼻咽癌组织中巨噬细胞移动抑制因子和MMP-2、MMP-9表达的关系

目的　研究巨噬细胞移动抑制因子 (macrophagemigrationinhibitoryfactor,MIF)和MMP 2、MMP 9在鼻咽癌组织中的表达水平及相互关系 ,探讨鼻咽癌细胞早期侵袭转移的机制。方法　收集 4 5例确诊的鼻咽原发癌活检组织标本 ,采用免疫组化LSAB法检测鼻咽癌组织中MIF和MMP 2、MMP 9的表达 ,并分析患者的临床参数的关系。结果　在 4 5例鼻咽原发癌组织中 ,MIF、MMP 2和MMP 9的阳性表达率分别为 77 8% (35 / 4 5 )、6 4 4 % (2 9/ 4 5 )和 71 1% (32 / 4 5 )。其中 ,癌细胞MIF和MMP 9的表达水平均显示与淋巴结转移有关 ,伴有淋巴结转移的癌组织中二者表达水平均高于无淋巴结转移的癌组织 (P值均 <0 0 5 )。MIF阳性组的癌细胞MMP 9的表达 (5 0 2 %± 33 5 % )明显高于MIF阴性病例 (11 7%± 2 2 7% ) ,两者差异有显著性 (P <0 0 1) ,且MIF的表达与MMP 9的表达亦呈正相关 (rs=0 .4 92 ,P <0 0 1) ,但癌细胞MMP 2的表达与MIF、MMP 9的表达以及是否有淋巴结转移则均未显示相关性。以Schmincke型生长方式分布的癌细胞MIF表达水平 (6 7 4 %±35 2 % )也高于以Regaud型方式分布的癌细胞 (32 9%± 2 9 7% ) ,差异有显著性 (P <0 0 1)。结论　鼻咽癌组织中癌细胞的MIF和MMP 9同步过表达 ,可能在鼻咽癌细胞的转移     

血清白细胞介素-6、白细胞介素-17和基质金属蛋白酶9水平与一氧化碳中毒迟发性脑病的关系

目的 探讨白细胞介素(IL)-6)、IL-17和基质金属蛋白酶9(MMP9)与一氧化碳中毒迟发性脑病(DEACMP)的关系.方法 随机抽取58例体检健康人群为对照组,抽取71例DEACMP患者为实验组,采用酶联免疫吸附试验(ELISA)检测DEACMP患者血清中IL-6、IL-17和MMP9的表达水平.结果 和对照组相比,轻重度DEACMP患者血清中IL-6和MMP9表达水平升高(F=22.47、F=36.51,P＜0.05),其中在重度DEACMP患者血清中升高最显著;IL-17表达水平未有显著变化,差异无统计学意义(F=19.62,P＞0.05).Pearson相关分析显示,DEACMP患者血清IL-6和MMP9浓度呈正相关(r=0.58,P＜0.05).结论 DEACMP患者血清中IL-6和MMP9的表达水平与一氧化碳中毒的程度和预后密切相关.     

WNT信号抑制因子DKK3基因 对结直肠癌细胞系HCT116迁移与侵袭的影响

目的分析DKK3在结直肠癌组织中的表达及对患者预后的影响,同时探索DKK3对结直肠癌细胞增殖、迁移以及侵袭能力的影响,并对机制进行一定的探索。方法在线数据库分析DKK3在结直肠癌组织中的表达及其对患者预后的影响。用RNA干扰技术,下调DKK3;用MTT检测细胞增殖,同时运用细胞小室迁移实验检测细胞迁移及侵袭能力,并通过RT-qPCR技术以及Western blot技术检测迁移相关蛋白表达。结果与正常组织相比,DKK3在结直肠癌组织中的表达显著上调并会导致患者整体预后情况变差(P0.05),干涉DKK3后细胞增殖速率明显减缓(P0.05),并且细胞迁移以及侵袭能力减弱(P0.01)。MMP2、 MMP7以及MMP9与DKK3的表达呈正相关关系(P0.001)。在敲低DKK3后,MMP2、 MMP7、 MMP9以及p-ERK的表达均显著下调(P0.01)。结论 DKK3在结直肠癌细胞系HCT116中表达上调,可能通过MAPK/ERK信号通路促进细胞增殖以及迁移能力。     

风湿性心脏病心房颤动患者心房组织基质金属蛋白酶表达的研究

目的:检测风湿性心脏病心房颤动(房颤)患者心房组织中基质金属蛋白酶（MMP1，MMP9）及其组织抑制因子（TIMP1）表达的变化及血浆MMP1，MMP9、TIMP1水平的变化，并作相关分析。 方法:56例风湿性心脏病二尖瓣病变患者分为3组，窦性心律组、阵发性房颤组和持续性房颤组，于心脏外科手术时取右心耳组织，采用逆转录-聚合酶链反应（RT-PCR）检测MMP1，MMP9及TIMP1 mRNA的含量，同时取静脉血检测外周血MMP1，MMP9和TIMP1水平。 结果:阵发性房颤组、持续性房颤组MMP9、TIMP1表达显著大于窦性心律组(P<0.05 or P<0.01)，TIMP1/MMP1比值明显升高(P<0.05 or P<0.01)，持续性房颤组TIMP1/MMP1比值明显高于阵发性房颤组(P<0.05 )；阵发性房颤组、持续性房颤组外周血MMP9、TIMP1水平及TIMP1/MMP1明显升高(P<0.05 or P<0.01)，与心肌组织表达水平相一致。相关分析显示，血浆MMP1，MMP9、TIMP1与心肌组织MMP1，MMP9、TIMP1显著正相关（r=0.71, P<0.01），MMP9、TIMP1与房颤时间正相关(r=0.68, P<0.01)。 结论:风湿性心脏病患者心肌组织MMP9、TIMP1表达改变及TIMP1/MMP1比值改变可能是心房纤维化发生的分子机制之一，检测外周血MMP1，MMP9及TIMP1变化可了解其在心肌组织的表达情况。     

基质金属蛋白酶及其抑制剂在乳腺癌转移、浸润中的作用

目的 :探讨基质金属蛋白酶 (MMP 2、MMP 9)及其抑制剂 (TIMP 1、TIMP 2 )与乳腺癌的关系。方法 :应用免疫组化S P法检测乳腺癌中MMP 2、MMP 9、TIMP 1、TIMP 2的表达。结果　 30例乳腺癌患者中MMP 2阳性表达 14例 ,占 46 7% ,MMP 9阳性表达 16例占 5 3 3% ,TIMP 1阳性表达 4例 ,占 13 3% ,TIMP 2阳性表达 3例 ,占 10 0 %。MMP 2、MMP 9的表达与乳腺癌的淋巴结状况、肿瘤大小相关 ,与年龄、月经状况无关。结论 :MMP 2、MMP 9的表达可以作为估计乳腺癌预后及术后综合治疗的生物学指标     

MMP11在浆液性卵巢癌中的表达、临床意义及其表位的预测

目的 检测MMP11在正常卵巢组织与浆液性卵巢癌组织中的表达及临床意义,筛选用于设计抗肿瘤多肽疫苗的候选表位.方法 免疫组织化学和GEPIA平台分析MMP11蛋白及mRNA在卵巢癌组织和正常卵巢组织中的表达,探讨其与浆液性卵巢癌患者的临床病理参数的关系;Kaplan Meier plotter分析MMP11 mRNA表达与患者预后的相关性;基因富集分析预测MMP11的功能;IEDB数据库及MOE分子模拟软件筛选潜在的MMP11的HLA-A*0201限制性表位.结果 MMP11 mRNA与蛋白在浆液性卵巢癌组织中表达水平显著高于正常组织(P＜0.05)且与肿瘤分期、大小及淋巴结浸润相关;MMP11 mRNA表达水平高的患者总体生存期和无病生存期较短(P＜0.05);MMP11主要富集于肿瘤相关通路与T细胞免疫功能相关基因子集;候选表位RV和LL具有与HLA-A*0201分子结合的潜力.结论 MMP11在浆液性卵巢癌的发生和发展中发挥重要作用,是一个潜在的浆液性卵巢癌的生物标志物和免疫治疗靶点.     

二甲双胍抑制UVA诱导的人皮肤成纤维细胞的光老化效应

目的研究二甲双胍(MF)对长波紫外线(UVA)诱导的人皮肤成纤维细胞(HSF)光老化效应的抑制作用及相关机制。方法体外培养人皮肤成纤维细胞,分为对照组(control)、UVA组、UVA+MF组。以CCK-8法检测细胞增殖;对各组细胞进行细胞衰老β-半乳糖苷酶染色;以荧光探针DCF-DA染色流式细胞仪检测细胞内ROS水平;real-time PCR检测衰老相关基因MMP1、MMP3的mRNA相对表达量;Western blot检测蛋白MMP1、MMP3、SOD1和SOD2的表达。结果 0.01 mmol/L二甲双胍对HSF增殖无显著影响,0.1和1 mmol/L二甲双胍则显著抑制HSF的细胞增殖(P0.05)。与对照组相比,UVA组β-半乳糖苷酶染色阳性率显著增高(P0.01);ROS水平显著升高(P0.05);MMP1和MMP3 mRNA相对表达量显著增加(P0.01);MMP1和MMP3蛋白表达量显著增多(P0.01);SOD1、SOD2蛋白表达显著减少(P0.01)。与UVA组相比,UVA+MF组β-半乳糖苷酶染色阳性率显著降低(P0.05);ROS水平显著下降(P0.05);MMP1和MMP3的mRNA相对表达量显著降低(P0.05);蛋白MMP1和MMP3表达量显著降低(P0.05);蛋白SOD1表达显著增加(P0.01);SOD2表达量增加。结论二甲双胍可通过抑制细胞内ROS水平和相关基质金属蛋白酶的表达,提高细胞抗氧化能力,从而抑制UVA诱导的皮肤成纤维细胞的光老化效应。     

胃癌组织中miR-107、AXIN2的表达及与预后的相关性

目的检测微小RNA-107(microRNA-107, miR-107)和轴抑制蛋白2(axis inhibition protein 2, AXIN2)在胃癌组织中的表达,探讨两者表达的关系及临床意义。方法采用qRT-PCR法检测65例胃癌及正常胃黏膜组织中miR-107和AXIN2 mRNA表达水平;采用免疫组化SP法检测AXIN2蛋白表达水平,分析miR-107、AXIN2与胃癌临床病理特征的关系;Kaplan-Meier法分析胃癌患者术后3年生存期;Cox生存回归分析影响胃癌患者预后的危险因素;Pearson分析胃癌组织中miR-107与AXIN2 mRNA表达水平的相关性。结果胃癌组织中miR-107的表达水平高于正常胃黏膜组织(P0.05),AXIN2 mRNA表达水平及蛋白阳性率均低于正常胃黏膜组织(P0.05);miR-107和AXIN2表达与肿瘤分化程度、淋巴结转移、TNM分期及浸润深度有关(P0.05);miR-107高表达组患者的3年生存率显著低于miR-107低表达组患者,AXIN2高表达组胃癌患者3年生存率显著高于低表达组患者(P0.05);淋巴结转移、TNM分期、miR-107高表达、AXIN2低表达是影响胃癌患者不良预后的独立危险因素(P0.05);胃癌组织中miR-107与AXIN2 mRNA表达呈负相关(r=-0.607,P0.001)。结论胃癌组织中miR-107高表达、AXIN2低表达,且与患者预后有关,可能作为胃癌患者潜在的预后标志物。     

Concurrent alterations of RAGE,RECK, and MMP9 protein expression are relevant to Epstein-Barr virus infection,metastasis, and survival in nasopharyngeal carcinoma

This study aimed to concurrently investigate the expressions of receptor for advanced glycation end products (RAGE), reversion inducing cysteine-rich protein with Kazal motifs (RECK) and matrix metalloproteinase 9 (MMP9) in nasopharyngeal carcinoma (NPC) and their correlations with clinicopathological properties. Using immunohistochemistry, we found that RECK expression was downregulated in NPC tissues compared with chronic nasopharyngitis (CNT) tissues, while RAGE and MMP9 expressions were upregulated. We further found that RECK expression level was inversely correlated with MMP9 expression level in NPC, whereas RAGE expression level was positively correlated with MMP9 expression level. Moreover, aberrant expressions of these proteins had a positive correlation with the titers of EBVCA-IgA, lymphatic metastasis, recurrence and survival. Together, these findings suggest that dysregulations of RECK and RAGE expressions may be collectively involved in tumor progression of NPC by regulating MMP9 expression and that they may be a good prognostic predictors for NPC.     

Nuclear overexpression of metastasis-associated protein 1 correlates significantly with poor survival in nasopharyngeal carcinoma

ABSTRACT: BACKGROUND: Metastasis-associated protein 1 (MTA1) has been associated with poor prognosis in several malignant carcinomas. The purpose of this study was to investigate the expression and prognostic value of MTA1 in nasopharyngeal carcinoma (NPC). METHODS: MTA1 expression was assessed using immunohistochemistry in paraffin-embedded tumor specimens from 208 untreated NPC patients. Cox regression analysis was used to calculate the hazard ratio (HR), 95% confidence interval (CI) and identify independent prognostic factors, and recursive partitioning analysis was used to create a decision tree. RESULTS: Nuclear overexpression of MTA1 was observed in 48.6% (101/208) of the NPC tissues. Nuclear overexpression of MTA1 correlated positively with N classification (P = 0.02), clinical stage (P = 0.04), distant metastasis (P < 0.01) and death (P = 0.01). Additionally, nuclear overexpression of MTA1 correlated significantly with poorer distant metastasis-free survival (DMFS; P <0.01) and poorer overall survival (OS; P < 0.01). MTA1 had prognostic significance in NPC patients with stage II disease, but not stage III or IV disease. Multivariate analysis demonstrated that nuclear overexpression of MTA1 was independently associated with poorer DMFS (HR, 2.05; 95% CI, 1.13-3.72; P = 0.02) and poorer OS (HR, 1.98; 95% CI, 1.09-3.59; P = 0.03). Using recursive partitioning analysis, the NPC patients could be classified with a low, intermediate or high risk of distant metastasis and death, on the basis of clinical stage, age and MTA1 expression. CONCLUSION: The results of this study suggest that nuclear overexpression of MTA1 correlates significantly with poorer DMFS and poorer OS in NPC. MTA1 has potential as a novel prognostic biomarker in NPC.     

胆囊癌组织P62蛋白的表达及其与血管生成关系

目的 :检测胆囊癌组织中P6 2蛋白、碱性成纤维细胞生长因子(bFGF)的表达和微血管密度 (MVD) ,探讨它们的相互关系 ,以及与胆囊癌临床病理特征的关系。方法 :4 1例胆囊癌和 2 2例慢性胆囊炎组织中 ,P6 2蛋白和bFGF的表达用SP免疫组化染色法进行检测。胆囊癌组织中MVD用抗CD34单抗 (mAb)做SP免疫组化染色进行检测。结果 :4 1例胆囊癌组织中 ,P6 2和bFGF表达阳性率分别为 6 3.4 %和 75 .6 % ,均高于 2 2例慢性胆囊炎组织 (P <0 .0 1)。P6 2的表达与胆囊癌淋巴结转移有关 (P <0 .0 5 ) ,与癌组织的分级、临床分期无显著关系。bFGF的表达与胆囊癌临床病理分期及组织学分级有关 (P <0 .0 5 ) ,而与淋巴结转移无显著关系。P6 2表达率与bFGF表达率呈正相关关系 (r=0 .5 2 1;P <0 .0 1)。两者的表达均与患者的年龄、性别、肿瘤的种类、是否伴有胆囊结石无关。胆囊癌组织中MVD明显高于慢性胆囊炎组织 (P <0 .0 1)。P6 2及bFGF表达阳性组织MVD高于表达阴性组织 (分别P <0 .0 5和P <0 .0 1)。MVD与胆囊癌的Nevin分期及淋巴结转移有关 (P<0 .0 1) ,与患者的年龄、性别、肿瘤种类、分化程度以及是否伴有胆囊结石无关。结论 :P6 2蛋白及bFGF的表达与胆囊癌组织中血管的生成相关 ,在胆囊癌的发生和发展过程中可能具有重     

Long-term follow-up and prognostic significance of angiogenic basic fibroblast growth factor (bFGF) expression in patients with breast cancer

The development of distant metastasis in breast cancer patients is the key step towards worse prognosis. The angiogenic basic fibroblast growth factor (bFGF) has been associated with tumorigenesis and metastasis in several human cancers. Therefore, bFGF expression was studied by immunohistochemistry in 111 patients with primary breast cancer. The results were correlated with prognostically relevant clinico-pathological features. such as tumor stage, grading. nodal stage and survival. bFGF was expressed in approximately 70% of the breast cancer tissues; 30% of the tumors showed strongly positive staining. With the exception of histological grading (p < 0.05), no correlation was found between the extent of bFGF expression and prognostic parameters. Analysis of survival showed a significantly (p < 0.05) prolonged survival for patients with a concomitant absence of axillary lymph node metastasis and bFGF immunoreactivity. Our data suggest that increased bFGF expression is a novel parameter for worse prognosis in nodal-negative breast cancer patients.     

Nuclear p27 Expression Confers a Favorable Outcome for Nasopharyngeal Carcinoma Patients

Objective. The purpose of the present study is to explore the correlation between nuclear expression of cyclin-dependent kinase inhibitor 1B (p27) and clinicopathologic features in nasopharyngeal carcinoma (NPC), including patient survival. Methods. Immunohistochemistry was used to examine the expression of p27 in 130 primary NPC tissues. The relationship between the levels of p27 expression and clinicopathologic characteristics was analyzed. Survival curves were plotted using the Kaplan-Meier method and compared using the log-rank test. The significance of various survival variables was analyzed using multivariate Cox proportional hazards model. Results. p27 was expressed in both nuclear and cytoplasmic compartments. Nuclear expression of p27 was inversely correlated with T classification and clinical stage. Patients with nuclear p27 expression had better overall survival rates than those without nuclear expression of p27. Further, we observed that nuclear expression of p27 was positively associated with survival time of NPC patients not only in N0-1 and M0 classifications but also in radiotherapy and chemotherapy treatment groups. Finally, we found that nuclear expression of p27 was not an independent prognostic factor for patients with NPC. Conclusions. Our findings hint that nuclear expression of p27 is a potentially favorable factor in the progression and prognosis of NPC.     

Expression of Matrix Metalloproteinase‐9 mRNA and Vascular Endothelial Growth Factor Protein in Gastric Carcinoma and Its Relationship to Its Pathological Features and Prognosis

To investigate matrix metalloproteinase‐9 (MMP‐9) mRNA and vascular endothelial growth factor (VEGF) protein expression in gastric carcinoma and its correlation with microvascular density, growth‐pattern, invasion, metastasis, and prognosis. In situ hybridization of MMP‐9 mRNA and immunohistochemistry of VEGF and CD34 proteins were performed on surgical specimens of gastric cancers from 118 patients compared with 20 nonmalignant gastric mucosae. Their relationships to pathological parameters and survival times were determined by statistical analysis. The positive rate of MMP‐9 in noncancerous gastric mucosae was significantly lower than that of gastric cancer tissue (60.17%, P < 0.01). In patients with cancers of the infiltrating type, at stage T3‐T4, with vessel invasion, lymphatic metastasis, hepatic, or peritoneal metastasis, the positive expression rates of MMP‐9 mRNA, VEGF protein, and CD34 were significantly higher than those for patients with tumors of the expanding type (P < 0.01), at stage T1–T2 (P < 0.01), with nonvessel invasion (P < 0.05), without lymphatic metastasis (P < 0.05), and without hepatic (P < 0.001) or peritoneal metastasis (P < 0.001), respectively. Expression of MMP‐9 mRNA was positively related to that of VEGF protein (P < 0.001) and microvascular density (P < 0.001). Patients with higher MMP‐9 mRNA and VEGF expression demonstrated vivid tumor angiogenesis and poor 5‐year survival rate. MMP‐9 and VEGF expression is associated with enhanced tumor angiogenesis and may play crucial roles in the invasion and metastasis of gastric carcinoma. Therefore, MMP‐9 and VEGF may represent prognostic biomarkers and promising targets for therapeutic intervention. Anat Rec, 2010. © 2010 Wiley‐Liss, Inc.     

Abnormal expression of matrix metalloproteinase-9 (MMP9) correlates with clinical course in Chinese patients with endometrial cancer

Aims and background: The aim of the present study was to analyze the expression of matrix metalloproteinase-9 (MMP9) in endometrial cancer and its correlation with clinicopathologic features in Chinese patients, including the survival of patients with endometrial cancer. Methods: Using immunohistochemistry analysis, we analyzed MMP9 protein expression in clinicopathologically characterized 128 endometrial cancer (EC) cases with age ranging from 30 to 85 years (median=51.6 years) and 30 endometrial atypical hyperplasia (EAH) and 30 normal endometrium (NE). Cases with greater than or equal to 6 and less than 6 with the score value of cytoplasmic MMP9 immunostaining were regarded as high expression and low expression, respectively. The relationship between the expression levels of MMP9 and clinical features was analyzed in EC cases. Results: Immunohistochemical analysis revealed that the protein expression of MMP9 detected in EC tissues was higher than that in the EAH tissues and NE tissues (P=0.006). In addition, high levels of MMP9 protein were positively correlated with the status of lymph node metastasis (P=0.044) and the histopathological grade (P<0.05) of EC patients. Patients with higher MMP9 expression did not correlate with EC patients'' clinical outcome in China. Multivariate analysis suggested that status of lymph node metastasis and depth of myometrial invasion, but not MMP9 expression level, were significantly correlated with patients'' survival. Conclusion: MMP9 is highly expressed in ECs and correlates with the progression of ECs, but not be helpful in predicting the prognosis of EC patients.     

Expression of heat shock protein 70 in nasopharyngeal carcinomas: different expression patterns correlate with distinct clinical prognosis

ABSTRACT: BACKGROUND: Heat shock protein 70, a stress protein, has been implicated in tumor progression. However, its role in nasopharyngeal carcinoma (NPC) progression has not yet been clearly investigated. METHODS: Immunohistochemistry (IHC) was employed to examine the expression patterns of Hsp70, human leukocyte antigen -A (HLA-A) in NPC tissue samples. RESULTS: The expression of Hsp70 exhibited different spatial patterns among nuclear, membrane and cytoplasm in 507 NPC tumor tissues. Kaplan-Meier survival analysis demonstrated that different Hsp70 expression patterns are correlated with different patient outcomes. High membranal and cytoplasmic levels of Hsp70 predicted good survival of patients. In contrast, high nuclear abundance of Hsp70 correlated with poor survival. Moreover, the membranal and cytoplasmic levels of Hsp70 were positively correlated with levels of the MHC I molecule HLA-A. CONCLUSIONS: Different Hsp70 expression patterns had distinct predictive values. The different spatial abundance of Hsp70 may imply its important role in NPC development and provide insight for the development of novel therapeutic strategies involving immunotherapy for NPC.