eIF4E在上皮性卵巢癌中的表达及意义

目的研究eIF4E在上皮性卵巢癌组织中的表达与卵巢癌发生、发展及预后的关系。方法应用免疫组化的方法分别检测32例上皮性卵巢癌、22例交界性卵巢癌、32例良性卵巢包块及20例正常卵巢组织中eIF4E的表达情况。结果卵巢癌组织中eIF4E的阳性表达率在晚期卵巢癌中比早期卵巢癌高(P0.05,r=0.855),有淋巴结转移组比无淋巴结转移组高(r=0.665,P0.05),而病理级别高低与eIF4E的阳性表达率无关(r=0.184,P0.05)。结论 eIF4E可能参与了卵巢癌的发生及发展过程,通过监测eIF4E的表达,可能帮助判断肿瘤的恶性程度。     

PTK7在食管鳞状细胞癌中的表达及临床意义

目的研究酪氨酸蛋白激酶-7（PTK7）在食管鳞状细胞癌、癌旁组织中的表达及其与食管鳞状细胞癌的发展、侵袭、淋巴结转移的关系,分析其在食管鳞癌中的诊断价值及预后变化。方法收集食管鳞状细胞癌标本80例及癌旁组织63例,采用免疫组织化学链霉菌抗生物素蛋白一过氧化物酶连结（SP）法检测PTK7蛋白的表达,结合临床相关因素进行x2检验及Kaplan—meier等统计学分析。结果80例患者食管鳞状细胞癌组织中有45例PTK7蛋白表达阳性（X2=50．17,P〈0．01）,食管癌旁非典型增生及正常鳞状上皮中PTK7蛋白表达均阴性。PTK7表达与食管鳞癌的年龄、性别、分化程度、浸润深度、淋巴结转移、浸润长度、临床分期等比较差异无统计学意义（P〉0．05）;与5年生存率比较差异亦无统计学意义（X2=0．2,P〉0．1）;当肿瘤组织PTK7蛋白表达综合免疫组化评分≥5分时,5年生存率有下降趋势（X2=3．35,P=0．06）。结论PTK7表达与食管鳞癌有关,当PTK7综合免疫评分≥5分时,5年生存率有下降趋势。利用检测PTK7的表达,有助于综合判断食管鳞癌的相关性和临床预后。     

食管鳞状细胞癌中FBXO32基因表达及其临床意义

目的观察食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)中FBXO32基因的表达,探讨该基因在ESCC发生、发展中的作用。方法分别应用RT-PCR及免疫组织/细胞化学法检测食管癌细胞和ESCC组织中FBXO32 mRNA及蛋白表达,观察DNA去甲基化剂5-氮杂-2’-脱氧胞苷(5-aza-2’-deoxycitydine,5-aza-dC)对食管癌细胞系(TE1、TE13、T.TN、Yes-2)FBXO32基因表达的影响。结果 TE13、T.TN、Yes-2细胞株中均未检测到FBXO32 mRNA及蛋白表达,在去甲基化剂作用后,4株食管癌细胞株中FBXO32基因表达均上调。FBXO32 mRNA在ESCC和癌旁正常组织中的表达量分别为0.24±0.15和0.49±0.21,ESCC组织中FBXO32 mRNA表达量明显低于癌旁正常组织(P<0.05),且与患者的淋巴结转移及肿瘤组织的分化程度密切相关。51例ESCC组织中,12例FBXO32基因蛋白表达,阳性率为23.5%,明显低于正常食管黏膜组织(70.5%,P<0.01),且FBXO32与患者的淋巴结转移密切相关。结论 FBXO32基因在ESCC中的异常低表达与ESCC的发生、发展密切相关,且甲基化可能是其表达沉默的机制之一。     

食管鳞状细胞癌间质细胞中podoplanin的表达及临床意义

目的观察食管鳞状细胞癌间质细胞中podoplanin的表达及与临床病理特征的关系,探讨podoplanin阳性间质细胞在食管鳞状细胞癌进展中的作用。方法应用免疫组化En Vision法检测69例食管鳞状细胞癌中podoplanin的表达,分析阳性间质细胞中podoplanin表达与食管鳞状细胞癌临床病理特征的关系。结果 69例食管鳞状细胞癌中,间质细胞podoplanin阳性者43例,阳性率为62.3%。食管鳞状细胞癌中间质细胞podoplanin阳性者淋巴管密度(lymphatic vessel density,LVD)为45.72±14.36,明显高于间质细胞podoplanin阴性者(21.23±10.49),差异有统计学意义(P<0.01);有淋巴管侵犯的食管鳞状细胞癌间质细胞podoplanin表达明显高于无淋巴管侵犯者(P<0.05)。间质细胞podoplanin表达与患者年龄、性别、肿瘤大小、浸润深度、分化程度、淋巴结转移和临床分期均无关(P>0.05)。结论 podoplanin阳性间质细胞可促进食管鳞状细胞癌淋巴管形成和淋巴管侵犯。     

P53和Id2蛋白在食管鳞状细胞癌的表达及其意义

目的研究P53、Id2蛋白在食管鳞状细胞癌组织的表达及其意义。方法采用免疫组织化学SP法和图像分析技术检测122例食管鳞状细胞癌及90例癌旁正常组织中P53、Id2的表达情况。结果 122例食管鳞状细胞癌中P53、Id2表达水平均高于癌旁正常组织(p0.01)。P53表达强度与患者的性别、年龄及肿瘤的分化程度未见明显相关性(p0.05),但与肿瘤的浸润深度及淋巴结转移情况相关(p0.05);Id2的表达与患者的性别、年龄及淋巴结转移情况未见明显相关性(p0.05),但与肿瘤的分化程度成负相关(p0.05),且与肿瘤浸润深度正相关(p0.05)。结论 P53、Id2在食管鳞状细胞癌的高表达可能作为判断食管鳞状细胞癌生物学行为的潜在指标。     

HAb18G/CD147在食管鳞状细胞癌中的表达及其临床意义

目的:探讨HAb18G基因产物表达与食管鳞状细胞癌(ESCC)临床病理特征及预后的关系。方法:应用免疫组化SP染色法分析了108例食管鳞状细胞癌标本中HAb18G基因产物表达情况和定位,并对其进行了术后3年随访。结果:HAb18G基因产物在有淋巴结癌转移和预后差的食管鳞状细胞癌病例中高表达;HAb18G的高表达与食管鳞状细胞癌的浸润转移、分化程度均相关(P<0.05);淋巴结转移和HAb18G表达可作为食管鳞状细胞癌患者预后独立指标。结论:HAb18G在食管鳞状细胞癌中的表达与食管癌的发生发展、浸润转移和预后密切相关,可作为临床预测浸润转移和估计预后的一个重要参考指标。     

食管鳞状细胞癌组织中miR-21与ERK1/2表达及临床意义的研究

目的：研究miR-21和ERK1/2在食管鳞状细胞癌(esophageal squamous carcinoma,ESCC)组织中的表达并分析二者的临床意义。方法：原位杂交技术和免疫组化方法分别检测80例食管鳞状细胞癌及癌旁正常组织中miR-21和ERK1/2的表达,Spearman相关统计方法分析miR-21和ERK1/2表达的相关性,并分析二者与ESCC患者临床病理资料的关系。结果：miR-21和ERK1/2在ESCC组织中的阳性表达率和表达量都显著高于癌旁正常组织,尤其在有淋巴结转移的ESCC组织中显著增高,但在ESCC不同性别、年龄、民族、病理类型、肿瘤侵及范围和肿瘤大小间的差异无统计学意义。进一步分析发现miR-21与磷酸化的ERK1/2(p-ERK1/2)在ESCC及癌旁正常组织的表达呈正相关,相关系数为0.570。结论：miR-21和p-ERK1/2与ESCC发生发展密切相关,二者可能协同通过促进肿瘤细胞淋巴结转移增加ESCC的恶性程度。     

TPX2在肺鳞状细胞癌及其癌前病变中的表达和意义

目的探讨Xklp2靶蛋白（targeting protein for Xklp2,TPX2）在肺鳞癌及其癌前病变中的表达和意义。方法Westernblot分析TPX2蛋白在2株肺鳞癌细胞系和4株永生化支气管上皮细胞系中的表达。逆转录聚合酶链反应分析21对新鲜肺鳞癌组织及其远端正常肺组织TPX2的mRNA表达水平。构建肺癌组织微阵列,针对其中的319例肺鳞癌患者的组织样品及其相应的114例癌前病变组织的常规石蜡切片进行TPX2免疫组织化学（SP法）染色,结果与临床病理参数比较分析。结果TPX2蛋白在被测肺鳞癌和永生化支气管上皮细胞系中均有水平不一的表达。在新鲜组织标本中76．2％的肿瘤组织较正常组织TPX2 mRNA明显高表达。免疫组织化学分析结果显示,TPX2蛋白的表达在肿瘤组织（64．2％）中明显高于正常组织,且与肿瘤组织病理学分级、临床分期及淋巴结转移相关。TPX2蛋白在肺鳞癌的癌前病变中的表达显著高于正常组织,且随支气管上皮病变程度加重（鳞状化生、不典型增生、原位癌）而增高。结论TPX2蛋白的表达有可能促进支气管上皮癌变和肺鳞癌进展,而且是肺鳞癌淋巴转移的危险因素。TPX2有望成为监测肺鳞癌发生发展的候选标志物。     

P53和1d2蛋白在食管鳞状细胞癌的表达及其意义

目的研究P53、Id2蛋白在食管鳞状细胞癌组织的表达及其意义。方法采用免疫组织化学SP法和图像分析技术检测122例食管鳞状细胞癌及90例癌旁正常组织中P53、Id2的表达情况。结果122例食管鳞状细胞癌中P53、Id2表达水平均高于癌旁正常组织（P〈0．01）。P53表达强度与患者的性别、年龄及肿瘤的分化程度未见明显相关性（P〉0．05）,但与肿瘤的浸润深度及淋巴结转移情况相关（P〈0．05）;Id2的表达与患者的性别、年龄及淋巴结转移情况未见明显相关性（P〉0．05）,但与肿瘤的分化程度成负相关（P〈0．05）,且与肿瘤浸润深度正相关（P〈0．05）。结论P53、Id2在食管鳞状细胞癌的高表达可能作为判断食管鳞状细胞癌生物学行为的潜在指标。     

CST4在哈萨克族食管鳞状细胞癌中的表达及意义

目的 探究半胱氨酸蛋白酶抑制剂S(CST4)在哈萨克族食管鳞状细胞癌(ESCC)中的表达情况及临床意义。方法 收集2010年至2020年于新疆医科大学第一附属医院49例接受手术治疗的哈萨克族食管鳞状细胞癌患者的新鲜组织标本及石蜡包埋的组织标本。用RT-qPCR及免疫组化法检测食管癌组织与对应癌旁组织中CST4的表达。用χ²检验分析CST4表达与患者临床病理资料之间的关系。用单因素及多因素分析CST4表达与患者术后生存期的关系。结果 CST4 mRNA在食管癌组织中的表达量明显高于对应癌旁组织(P<0.05)。CST4蛋白在食管癌组织中的表达率(53.1%, 26/49)明显高于对应癌旁组织(28.2%, 11/39)(χ²=5.506,P<0.05)。CST4表达与淋巴结转移(P<0.05)和TNM分期(P<0.01)有关,CST4高表达者淋巴结转移率更高,肿瘤TNM分期更晚。单因素生存分析发现,CST4表达水平(P<0.01)、肿瘤浸润深度(P<0.05)和TNM分期(P<0.01)均与患者生存率相...     

Mnk2蛋白水平与食管鳞癌预后的相关性

目的:检测丝裂原活化蛋白激酶相互作用激酶2(mitogen-activated protein kinase-interacting kinase-2,Mnk2)在食管鳞状细胞癌中的表达水平,并探讨其与患者生存预后的相关性。方法:收集临床食管鳞癌标本86例及癌旁正常食管组织54例,应用Western blot法和免疫组化SP法检测肿瘤组织及正常食管黏膜组织中Mnk2蛋白表达水平,并用Kaplan-Meier生存曲线和Cox比例风险回归模型的方法探究其与食管鳞癌患者预后的关系。结果:Mnk2在食管癌组织中呈高表达,并且Mnk2蛋白表达与食管鳞癌的TNM分期密切相关(P0.05),同时Mnk2蛋白高表达组的无疾病进展生存期和总生存期均少于Mnk2低表达组,多因素分析提示Mnk2是食管鳞癌的独立预后因子。结论:Mnk2在食管鳞癌组织中的表达与TNM分期有关,同时可作为预测食管鳞癌患者预后的指标。     

Nrf2在食管鳞癌组织中的表达及意义

目的:探讨Nrf2(Nuclear factor E2 p45-related factor2)在食管鳞癌组织中的表达及其与临床病理学特征的关系。方法:采用免疫组化SP法检测Nrf2在32例食管鳞癌,30例癌旁组织,21个阳性淋巴结和24个阴性淋巴结组织中的表达。结果:Nrf2阳性表达主要定位于细胞核中,在食管鳞癌中的阳性表达率为78.13%,显著高于癌旁组织(13.33%),淋巴结癌转移阳性组织中的表达率(66.67%)也显著高于淋巴结癌转移阴性组织中的表达水平(20.83%),均具有统计学意义(P<0.05)。Nrf2的阳性表达随淋巴结的转移度的增加而表达增加(P<0.05),但在不同年龄、性别、TNM分期、肿瘤分化程度及不同部位之间差异无统计学意义。结论:Nrf2在食管鳞癌中高表达,表达的高低与淋巴结转移与否及转移度有关。     

Expression and clinical significance of B cell translocation gene 2 in esophageal squamous cell carcinoma

Esophageal squamous cell carcinoma (ESCC) is widely known as a highly fatal cancer, and thus it is important to identify tumor-specific and radiosensitivity-specific markers in ESCC. B cell translocation gene 2 (BTG2) has been considered a novel tumor suppressor gene or radiotherapy sensitivity-associated gene. However, the relationship between BTG2 and ESCC development and radiotherapy sensitivity is uncertain. The present study aims to explore the expression and clinical significance of B cell translocation gene 2 (BTG2) in ESCC by analyzing the RNAseq data from the TCGA and immunohistochemical staining of ESCC samples. We found that the level of BTG2 mRNA was significantly decreased in ESCC patients, and further decreased significantly in radiotherapy resistant patients compared to sensitive patients. The positive expression rate of BTG2 protein was 56.0% (103/184) in 184 ESCC tissue samples and 84.0% (42/50) in normal esophageal mucosal samples, respectively. The positive ratios of BTG2 expression in radiotherapy-sensitive group and radiotherapy resistant group were 57.9% (22/38) and 23.5% (4/17), respectively. Furthermore, the analysis indicates that the expression level of BTG2 significantly correlated with lymph node metastasis and clinical staging in ESCC patients. A multivariate analysis with Cox regression model showed that BTG2 level was an independent risk factor affecting the prognosis of ESCC patients. Above all, the downregulation of BTG2 may be used as a molecular marker to identify and predict ESCC progression and radiosensitivity.     

Association of Kruppel-like factor 4 expression with the prognosis of esophageal squamous cell carcinoma patients

Objective: To investigate the association of Kruppel-like factor 4 (KLF4) expressions with the prognosis of esophageal squamous cell carcinoma (SCC) patients. Methods: Ninety-eight cases of esophageal carcinoma patients were enrolled. The expression of KLF4 in the esophageal SCC and normal esophageal mucosa tissues were examined by immunohistochemistry. The correlations between the expression of KLF4 protein and patients’ clinical characteristics and prognosis were analyzed. Results: We observed higher expressed KLF4 in normal esophageal mucosa tissues than esophageal SCC tissues, with positive rate of 82.7% (81/98) and 43.8% (43/98) respectively. In patients with lymphatic metastasis, the positive rate of KLF4 was 24.4% (10/41), whereas it was 57.9% (33/57) in patients without lymphatic metastasis, and the difference was significant (x² = 10.871, P = 0.001). The positive rates of KLF4 were 62.5% (5/8), 53.1% (26/49) and 29.3% (12/41) in stage I, II and III patients, respectively. There were no correlations between the expression of KLF4 and gender, age, tumor size, location, differentiation grade and infiltration depth. The 5-year survival rates and median survival times were 48.8% and 25.5%, and 55 and 26 months for the patients with KLF4 positive and negative expression, respectively. There were significant differences between the patients with KLF4 positive expression and negative expression in the 5-year survival rates and median survival times (x² = 5.747 and 4.493, P = 0.017 and 0.034). Conclusion: KLF4 might act as a tumor suppressor in esophageal SCC and the expression status of KLF4 could be considered as a prognosis predictor for esophageal SCC patients.     

骨桥蛋白在食管鳞癌中的表达及其临床意义

目的观察骨桥蛋白（OPN）在食管鳞癌组织、癌旁组织和转移淋巴结的表达状况,探讨OPN在食管鳞癌中表达的临床意义。方法运用免疫组化S-P法检测44例食管鳞癌组织、癌旁组织和20例转移淋巴结免疫组化染色。结果OPN在食管癌组织、癌旁组织及转移淋巴结中的表达率分别为86．3％、0％和100％。癌组织中OPN主要表达于肿瘤细胞的细胞浆中。OPN的表达与肿瘤TNM分期、淋巴结转移状态有关,而与肿瘤位置、肿瘤直径、浸润深度及病理学分级无关。OPN在癌组织、癌旁组织及转移淋巴结表达强度亦存在显著性差异。结论食管鳞癌组织中OPN主要由肿瘤细胞产生。OPN与肿瘤的浸润、转移有关,反映了肿瘤的生物学特性。     

C-erbB-2和VEGF在鼻腔-鼻窦鳞状细胞癌中的表达及临床意义

目的研究原癌蛋白(C-erb B-2)及血管内皮生长因子(VEGF)在鼻腔、鼻窦鳞状细胞癌(SNSCC)中的表达及其临床意义。方法收集62例SNSCC组织、30例鼻息肉组织(NP组)以及25例正常鼻腔黏膜(正常组),用免疫组化及Western blot技术分别检测C-erb B-2和VEGF在三者中的表达情况。结果 C-erb B-2和VEGF在SNSCC组织中的表达明显高于鼻息肉及正常鼻腔黏膜中的表达(P0.05)。另外,C-erb B-2和VEGF的表达与病理类型、临床病理分期及淋巴结转移等临床病理特征呈正相关(P0.05),而与性别、年龄无关。结论 C-erb B-2和VEGF与SNSCC的发生、分化及细胞增殖密切相关,联合检测二者有助于对SNSCC的诊断与治疗。     

Nuclear PKM2 expression predicts poor prognosis in patients with esophageal squamous cell carcinoma

Esophageal squamous cell carcinoma (ESCC) is one of the most common tumors worldwide, with a high malignant degree and poor prognosis. The present study aims to investigate the relationship between pyruvate kinase M2 (PKM2) expression and the prognosis of patients with ESCC. The expression of PKM2 in 86 cases of esophageal carcinoma tissues was tested using immunohistochemistry. The relationship between PKM2 expression and clinical pathological parameters, and their effects on the prognosis of patients with ESCC were analyzed. The expression levels of PKM2 in both cytoplasm and nucleus of ESCC tissues were significantly higher than those in paracancerous tissues (P = 6.73 × 10⁻⁹ and 4.32 × 10⁻⁶, respectively). The Kaplan–Meier analysis showed that nuclear PKM2 expression was closely related to the survival of patients with ESCC (P = 0.005). Patients with high PKM2 expression in the nucleus had significantly shorter survival times than those with low PKM2 expression in the nucleus (hazard ratio for death, 2.358; 95% confidence interval, 1.156–4.812; P = 0.018). No other significant difference was found between PMK2 expression and clinico-pathological features of ESCC patients (all P > 0.05). In conclusion, high PKM2 expression in the nucleus is essential in the pathogenic process of ESCC and may be used to predict the prognosis of patients with ESCC.     

PINCH expression and its significance in esophageal squamous cell carcinoma

Particularly interesting new cysteine-histidine rich protein (PINCH), as a newly discovered protein of LIM family members, may play a role in signal transduction of integrin and growth factor, and involved in the incidence and development of tumors. PINCH protein is overexpressed in tumor-associated stroma of several types of tumors. However, there is no study of the PINCH in esophageal cancer, therefore we investigated PINCH expression in esophageal squamous cell carcinomas and its clinicopathological significance in the patients. PINCH expression was immunohistochemically examined in 20 normal esophageal samples and 64 esophageal squamous cell carcinomas. The results showed that PINCH expression in the stroma of cancers was heterogeneous, and its positive rate (56%) was higher than that of normal esophageal mucosa (5%, p<0.0001). The stronger staining was observed at the invasive edge of tumor when compared to the inner area of tumor. The rate of positive PINCH (90%) in the cases with lymph node metastasis was higher than that (41%) in the cases without metastasis (p<0.0001). PINCH expression was not correlated with patients' gender, age, tumor location, size and differentiation (p>0.05). The results suggest that PINCH protein may be a marker of tumor associated-stroma involving tumor development, and predicting the ability of invasion and metastasis of esophageal squamous cell carcinoma.     

表皮生长因子受体在口腔鳞癌组织中的表达及意义

目的：研究表皮生长因子受体（ＥＧＦＲ）在口腔鳞癌组织中的表达及其临床病理学意义。方法：采用免疫组化Ｓ－Ｐ法对６５例不同分化的口腔鳞癌组织及１０例正常口腔粘膜组织进行ＥＧＦＲ检测。结果：ＥＧＦＲ在鳞癌组织中呈异质性表达,其阳性率（６１．５％）明显高于正常口腔粘膜组织（Ｐ〈０．０１）;ＥＧＦＲ表达状况与鳞癌组织学分级,区域淋巴结转移及患者预后间存在相关关系（Ｐ〈０．０１或０．０５）。结论：ＥＧＦＲ在口