血清β_2微球蛋白检验在糖尿病肾病患者中的临床价值分析

目的:探讨血清β_2微球蛋白检验在糖尿病肾病患者中的临床价值。方法:选取在我院接受医治的糖尿病肾病患者60例,作实验组,选取时间为2016年11月-2017年11月,另选取同期在我院进行体检的健康者60例,作对照组。对比2组血清β_2微球蛋白水平和血清β_2微球蛋白阳性率。结果:实验组患者其血清β_2微球蛋白水平高于对照组,组间差异明显,P0.05;实验组患者血清β_2微球蛋白阳性率为88.33%(53/60),显著高于对照组阳性率(3.33%),P0.05。结论:在糖尿病肾病疾病诊断中,检验其血清β_2微球蛋白水平效果较为理想,值得广泛应用。     

血清胱抑素C、β_2微球蛋白诊断糖尿病早期肾损害的临床意义

目的:探讨血清胱抑素C、β_2微球蛋白诊断糖尿病早期肾损害的临床意义。方法:随机选取糖尿病早期肾损害患者25例作为观察组,另外选取糖尿病无肾损害患者25例作为对照组,观察两组患者的血清胱抑素C及血、尿β_2微球蛋白水平变化情况,并对阳性率和检测灵敏度做出比较。结果:观察组的血清胱抑素C及血、尿β_2微球蛋白水平及其阳性率均明显高于对照组,此外,与单纯的血清胱抑素C(76.0%)或β_2微球蛋白(64.0%)相比,二者联用时检测灵敏度更高,达到了88.0%,差异均有统计学意义(P<0.05)。结论:血清胱抑素C和β_2微球蛋白在糖尿病早期肾损害患者的诊断中有重要的临床指导意义。     

血清β_2—微球蛋白与大肠癌

用放射免疫法测定了36例大肠癌患者的血清β_2-微球蛋白,并设立了对照组,结果发现对照组血清β_2-微球蛋白在正常范围,大肠癌组增高,阳性率为56%,其水平符合正态分布.并就其临床意义和产生的机理进行探讨.     

消化系癌肿与非癌肿疾病患者血清β_2—微球蛋白的变化及其临床意义

本文测定了40例消化系癌肿(26例)和非癌肿患者(14例)的血清β_2—微球蛋白(β_2MG)水平。结果发现消化系癌和非癌患者的血清β_2—MG均有明显增高,其中以消化系非癌肿组患者血清β_2—MG的增高最为显著,与癌肿组相比,有显著性差异(P<0.05)。如果以血清β_2—MG>3ug/dl作为判断异常的标准,消化系癌肿和非癌肿组的差异阳性率分别为53.8%和95.7%,但两组间无显著性差异(P<0.05)。因此单凭血清β_2—MG增高不能鉴别消化系疾病的良愿性,必须结合临床其它有关检查,方能作出良愿性的鉴别诊断。     

检测肺心病患者β_2-微球蛋白、Tamm-Horsfall蛋白及白蛋白的临床意义

作者采用放射免疫方法测定28例肺心病急性加重期患者血清β_2-微球蛋白(β_2-MG)、Tamm-Horsfall蛋白(THP)及24 h尿β_2-MG、THP和白蛋白(Alb)水平,结果表明,肺心病组血清和尿THP含量显著降低(P<0.05),血清、尿β_2-MG及尿Alb浓度显著升高(P<0.01),后三者阳性率显著高于血清中尿素氮及肌酐的阳性率,提示肺心病加重期患者的肾小球及肾小管功能均有不同程度损害.动态观察血清、尿β_2-MG和THP,及尿Alb变化,是判断其早期肾功能损害的敏感方法.     

急性白血病血清β_2微球蛋白的变化及来源探讨

应用放射免疫测定法分别对健康人、急性白血病病人血清与骨髓细胞内β_2微球蛋白含量变化进行了观察,并同时对正常人外周血淋巴细胞进行体外培养,检测其培养液上清液的β_2微球蛋白水平.结果表明,急性白血病病人治疗前及复发时血清β_2微球蛋白明显升高,完全缓解时下降至正常.同期骨髓单个核细胞内β_2微球蛋白(BM-β_2M)含量正常,与血清β_2微球蛋白(S-β_2M)升降无关.经植物血凝素(PHA)刺激后的淋巴细胞产生β_2微球蛋白明显增加.提示急性白血病S-β_2M升高与白血病细胞本身无直接相关,可能与淋巴细胞合成的β_2微球蛋白增多有关.     

血清β_2-微球蛋白测定对鼻咽癌诊断的临床意义

测定21例鼻咽癌、10例鼻咽癌放疗后、10例良性肿瘤、18例非肿瘤和35例正常人的血清β_2-微球蛋白.结果21例初诊鼻咽癌患者血清β_2-微球蛋白为3.55±2.13mg/L,正常人为2.08±0.97mg/L(P<0.001).10例放疗后的鼻咽癌患者为2.40±0.75mg/L,9例良性肿瘤和18例非肿瘤组分别为2.30±0.95mg/L、2.56±0.22mg/L.     

自身免疫性甲状腺疾病血、尿β_2-微球蛋白水平及其临床意义

用放免法测定76例自身免疫性甲状腺疾病及正常对照组的血、尿β_2-微球蛋白水平。结果:Graves病未缓解组、桥本氏病组血、尿β_2-微球蛋白较对照组明显升高(P<0.001),Graves病缓解组、特发性甲减组与对照组差异无显著性(P>0.05)。Graves病缓解组较Graves病未缓解组明显降低(P<0.01)。提示β_2-微球蛋白水平测定可做为自身免疫性甲状腺疾病诊断、疗效观察及了解其免疫状态的一项指标。     

甲巯咪唑治疗甲亢对β_2-球蛋白和甲状腺激素表达水平的影响

目的通过观测甲巯咪唑在治疗甲亢患者过程中β2-球蛋白含量与甲状腺激素含量的变化情况,研究两者的相关性及甲巯咪唑的药理学作用机制。方法随机选取73例甲状腺功能亢进症患者作为患者组,并且随机调取在本院进行身体健康检查的70名健康人体检资料作为对照组,给予73例患者甲巯咪唑作为甲亢治疗方案药物,在治疗过程中监测甲状腺激素水平变化情况以及使用放射免疫法(RIA)检测各组β2-球蛋白水平,并进行数据资料分析。结果在应用甲巯咪唑治疗甲亢的过程中,T4和T3水平升高组患者血清中β2-球蛋白水平(2764.15±179.67)明显高于对照组(1838.82±155.29)。在治疗过程中甲巯咪唑对甲状腺激素水平变化无明显影响,对β2-球蛋白含量存在一定影响,差异无统计学意义(P0.05)。结论甲状腺功能亢进症患者血清中β2-球蛋白含量与甲状腺激素水平存在密切关系,β2-球蛋白含量可以作为甲巯咪唑的药理学作用及甲亢患者的临床疗效检测指标。     

血液病患者血清β_2—微球蛋白测定的临床意义

用放射免疫法测定不同类型血液病患者77例及正常人45例血清β_2—微球蛋白(β_2—MG),结果表明,急、慢性白血病,恶性淋巴瘤患者β_2—MG值明显高于正常人(P<0.01),病情好转,其值下降,至完全缓解可降至正常水平;多发性骨髓瘤患者β_2—MG值高于正常(0.05>P>0.01);慢性再生障碍性贫血患者夕β_2—MG低于正常(P<0.01),随着病程延长其值有下降趋势,骨髓增生异常综合征、原发性血小板减少性紫癜、缺铁性贫血患者β_2—MG值在正常范围内。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.