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Development and evaluation of an IgM capture enzyme immunoassay for diagnosis of recent Norwalk virus infection 总被引:4,自引:0,他引:4
A capture enzyme immunoassay specific for Norwalk IgM class antibody was developed. The assay was moderately sensitive, identifying 33/53 (62%) of patients with naturally acquired Norwalk virus infection and 17/18 (94%) of experimentally infected volunteers. The assay was also specific for IgM class antibody and acute Norwalk virus infection and results were generally reproducible. A specific IgM response correlated with seroconversion by total antibody blocking assay and occurred independently of clinical symptoms. Among 81 symptomatic cases composing seven Norwalk outbreaks, specific IgM was absent from acute phase sera collected less than or equal to three days post onset, and was uncommon in sera collected within one week and after five weeks, with an optimal collection time at about two to three weeks. The Norwalk IgM capture immunoassay may be used to augment paired sera assays in the identification of Norwalk-associated outbreaks of acute gastroenteritis. 相似文献
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Development and validation of a negative-strand-specific reverse transcription-PCR assay for detection of a chicken strain of hepatitis E virus: identification of nonliver replication sites 下载免费PDF全文
Billam P Pierson FW Li W LeRoith T Duncan RB Meng XJ 《Journal of clinical microbiology》2008,46(8):2630-2634
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Comparison of a lateral-flow immunochromatography assay with real-time reverse transcription-PCR for detection of human metapneumovirus 下载免费PDF全文
Kikuta H Sakata C Gamo R Ishizaka A Koga Y Konno M Ogasawara Y Sawada H Taguchi Y Takahashi Y Yasuda K Ishiguro N Hayashi A Ishiko H Kobayashi K 《Journal of clinical microbiology》2008,46(3):928-932
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Highly sensitive assay for detection of enterovirus in clinical specimens by reverse transcription-PCR with an armored RNA internal control 下载免费PDF全文
Beld M Minnaar R Weel J Sol C Damen M van der Avoort H Wertheim-van Dillen P van Breda A Boom R 《Journal of clinical microbiology》2004,42(7):3059-3064
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Diagnostic evaluation of multiplexed reverse transcription-PCR microsphere array assay for detection of foot-and-mouth and look-alike disease viruses 下载免费PDF全文
Hindson BJ Reid SM Baker BR Ebert K Ferris NP Tammero LF Lenhoff RJ Naraghi-Arani P Vitalis EA Slezak TR Hullinger PJ King DP 《Journal of clinical microbiology》2008,46(3):1081-1089