免疫荧光检测阴道加德纳氏菌

加德纳氏菌（GV）是细茵性阴道病（BV）的主要病原体，可经性接触传播，由于该病菌生长条件苛刻，影响了该菌培养的开展。为此，我们应用免疫荧光和培养方法进行比较，现将结果报告如下。      

克氏耶尔森菌败血症1例

克氏耶尔森菌为非致病菌，由该菌致病的病例临床罕见。我院于2006年11月在一反复高热的患者血中培养出该菌，现报道如下。     

克氏耶尔森菌败血症1例

克氏耶尔森菌为非致病菌，由该菌致病的病例临床罕见。我院于2006年11月在一反复高热的患者血中培养出该菌，现报道如下。     

尖端赛多孢子菌引起骨髓移植患者局部感染1例

由于尖端赛多孢子菌（Scedosporium apiospermum）培养和鉴定难度大,发现该菌引起的感染病例较少,本文报道了国内首例急性髓性白血病患者行自体骨髓移植后由尖端赛多孢子菌所致左侧腹股沟感染。     

水体中致病菌快速检测的基因芯片技术研究

目的 探讨基因芯片技术行水体致病菌快速检测的方法.方法 采用基于单碱基延伸标签反应的基因芯片方法,对金黄色葡萄球菌、肠炎沙门菌、单核细胞增生李斯特菌、志贺菌、军团菌、肺炎克雷伯菌、肠出血性大肠埃希菌O157:H7、幽门螺杆菌、大肠埃希菌、霍乱弧菌、结核分枝杆菌、鼠疫耶尔森菌、炭疽芽孢杆菌等13种可能存在于水体中的致病菌进行检测.结果 该基因芯片可同时特异性地检测13种致病菌,基因组DNA检测灵敏度可达0.2pg,以肠炎沙门菌为单一检测对象的细菌纯培养物灵敏度可达1.0×103cfu/ml;配合浓缩集菌设备,整个检测时间可缩短至8h以内;用所制备的基因芯片检测模拟和实际水样,准确率达100%.结论 该基因芯片检测水体致病菌操作简单,速度快,灵敏度高,稳定性和重复性好.     

细菌培养标本加做L型菌培养452例药敏分析

 目的通过对452例送检标本做细菌常规培养并加做L型菌培养和药敏分析,了解L型菌的培养意义和耐药情况,为医生用药提供参考.方法对452例标本同时做两种培养,对检出的L型菌返祖后进行鉴定并做药物敏感试验.结果常规培养细菌的检出率为20.35%,常规培养阴性标本中,L型菌检出率为30%.两种培养的总检出率与常规培养的检出率相比有显著差异(P＜0.05).结论常规培养中未加做L型菌培养是导致临床感染漏诊、误诊的原因之一,L型菌培养不仅能提高病原菌的检出率,且能指导医生合理用药,临床上应得到重视.     

河生肠杆菌生物群2感染2例

河生肠杆菌（Enterobacter amnigenus）隶属于肠杆菌科肠杆菌属，较少从人的感染中分离到。我院先后自患者血液、腹腔渗液中培养出该菌。     

从患者血液中分离出痰塔特姆菌1例

塔特姆菌属（Tatumella）隶属于肠杆菌科，是Hollis等人在1981年提议而设立的新菌属。属内只有痰塔特姆菌（Tatumellaptyseos）1个种。已知的25个均来自临床标本，其中86％来自呼吸道，3株来自血液，是罕见的致病菌。我们从一患者血液中分离培养出该菌，报告如下。     

鼠疫耶尔森菌基因组进化与生态位适应研究

目的 探索鼠疫耶尔森菌基因组进化的基本规律及其与该菌在自然界适应性演化之间的内在联系。方法 对36株鼠疫耶尔森菌和7株假结核耶尔森菌进行芯片比较基因组分析,鉴定差异区段(DFR),并对260株鼠疫耶尔森菌进行PCR验证,分析各DFR在这些鼠疫耶尔森菌中的分布,同时进行基因组岛分析。结果 在鼠疫耶尔森菌基因组中鉴定出22个DFR,基于DFR图谱,将鼠疫耶尔森菌中国分离株分成14个基因组型。鼠疫耶尔森菌共有21个基因组岛,其中18个已存在于假结核杆菌中,余下3个为鼠疫耶尔森菌独有。结论 探明了各基因组岛在鼠疫耶尔森菌和假结核耶尔森菌间的差异分布,探究了鼠疫耶尔森菌的起源进化;建立了鼠疫耶尔森菌基因组分型系统;建立了各基因组型别间的系统发育关系,初步揭示了鼠疫耶尔森菌基因组的进化规律及其与鼠疫耶尔森菌生态位适应和鼠疫疫源地扩展之间的关系,基本探明了鼠疫耶尔森菌在中国的演化规律。     

痰菌阴性L型菌阳性的60例肺结核病人的临床分析

为了解痰菌阴性(-)L型菌阳性( )肺结核病人的临床表现和特点，对204例痰涂片及培养均阴性的肺结核病人做痰标本L型菌分离培养，依据L型菌分离培养的结果分为60例L型菌( )组及144例L型菌(-)组，并将其主要症状、X线表现及治疗结果进行比较。结果204例痰菌阴性肺结核L型菌检出率为29．4％，L型菌( )组的干酪病灶和干酪空洞多于L型菌(-)组，L型菌(-)组病灶的显著吸率(病灶吸收≥50％)高于L型菌( )组。作者认为，L型菌的存在提示病变有活动性，结核病的治疗必须坚持合理的全程化疗，直至痰中消灭L型菌。     

炭疽芽孢杆菌芽孢适配子的结构与亲和性分析

为了对炭疽芽孢杆菌疫苗株A．16R芽孢的适配子进行亲和性分析,体外构建了78个碱基的随机DNA库,以炭疽芽孢杆菌疫苗株A．16R芽孢为靶标,用SELEX技术进行了15轮的筛,针室的适配子库进行克隆,测序,用Macaw2.05和DNAsisv2.5软件对每一适配子的保守序列和二级结构进行分析,并用生物素－链酶亲和素－辣根酶系统检测,根据OD值的高低判定亲和力的大小,对每一适配子进行亲和力测定。结果表明,适配子的亲和力高低不一,OD值最高为1．2,最低为0．25,二级结构分析结果显示,茎环和茎等二级结构可是适配子与芽孢结合的基础;其一级结构提示有AGGGG,CCCCG,GGCTT、ACACT等保守序,上述分类与OD值的高低分布基本吻合。     

Cómo escribir un artículo. Cuestiones formales

Scientific research and the publication of the results of the studies go hand in hand. Exquisite research methods can only be adequately reflected in formal publication with the optimum structure. To ensure the success of this process, it is necessary to follow orderly steps, including selecting the journal in which to publish and following the instructions to authors strictly as well as the guidelines elaborated by diverse societies of editors and other institutions. It is also necessary to structure the contents of the article in a logical and attractive way and to use an accurate, clear, and concise style of language. Although not all the authors are directly involved in the actual writing, elaborating a scientific article is a collective undertaking that does not finish until the article is published. This article provides practical advice about formal and not-so-formal details to take into account when writing a scientific article as well as references that will help readers find more information in greater detail.     

Bone marrow edema: patterns and clinical implications

This article outlines the causes of bone marrow edema and classifies them by mechanism into congestive, vasogenic, traumatic, and tumorigenic forms. In particular, the article focuses on traumatic edema, emphasizing the differing patterns on the basis of mechanism of injury, impaction, distraction, and shear-type injuries. Finally the article reviews the impact of understanding mechanisms in image interpretation and in patient management.     

CT evaluation of chronic thromboembolic pulmonary hypertension

The educational objectives of this article are to provide an overview of the computed tomography (CT) findings in chronic thromboembolic pulmonary hypertension. This article reviews the key imaging findings at CT in patients with chronic thromboembolic pulmonary hypertension. After reading this article, the reader should have an improved awareness of the condition, its imaging features, and the CT imaging features associated with surgically accessible disease.     

颈椎病的X线诊断——附200例临床X线分析

本文总结了颈椎病２００例，从临床及Ｘ线表现进行了分析，并对Ｘ线诊断意义，病理机制，Ｘ线检查方法进行了讨论。     

Legal trends in imaging: an update

In 2004, a similarly titled article,"Legal Trends in Imaging," was published in this Journal; it described medical malpractice cases involving imaging, the role of imaging in criminal trials and evolving legal concerns raised by newer imaging technologies. This article provides an update on the ever-expanding relationship between the law and imaging. Readers are cautioned that nothing contained in this article is intended as legal advice and should not be taken as such.     

飞行人员用药安全与监测研究现状（摘要）

本文针对飞行员用药安全与监测进行了悉心的文献阅读与整理,就各国对飞行员如何带药飞行的研究做一综述。文中涉及到飞行员目前所用六大类药物,如抗抑郁药物、心血管药物、糖尿病药物、呼吸系统药物、抗组胺药物、中草药等。通过总结各国最新文献以阐述各国目前对飞行员安全带药的最新研究成果。作者亦对各国所做的飞行事故毒物检测结果进行了总结。     

The lumbar facet joint: a review of current knowledge: Part II: diagnosis and management

This article is the second article in a two-part review on lumbar facet joint pathology. In this review, we discuss the current concepts and controversies regarding the proper diagnosis and management of patients presenting with presumed facet-mediated lower back pain. All efforts were made to include the most relevant literature from the fields of radiology, orthopaedics, physiatry, and pain management. Our focus in this article is on presenting the evidence supporting or refuting the most commonly employed injection-based therapies for facet-mediated lower back pain.     

Radiologic-pathologic correlation of pediatric and adolescent spinal neoplasms: Part 1, Intramedullary spinal neoplasms

OBJECTIVE: The purpose of this article is to review the neuroimaging findings of pediatric and adolescent intramedullary spinal tumors in children. The differential diagnosis for lesions in this location is limited and can be further narrowed with knowledge of specific imaging characteristics. CONCLUSION: This article reviews the radiologic findings and differential diagnosis for intramedullary neoplasms. After completing this article, the reader should have an improved understanding of the types of neoplastic processes that can involve this region of the pediatric spine.     

先天性巨指(趾)畸形(附8例报告)

本文报告8例巨指(趾)症,其中6例行手术治疗。文中重点讨论了巨指(趾)症的X线鉴别诊断,结合文献复习对本病的病因临床表现及治疗方法加以讨论。