Ge－132赖氨酸盐的抗癌作用

Ｇｅ－１３２赖氨酸盐２００ｍｇ·ｋｇ－１·ｄ－１×１０ｄ及１００ｍｇ·ｋｇ－１·ｄ－１×１０ｄｉｇ对小鼠移植性Ｓ１８０荷瘤实体瘤的抑制率分别为４６．７％、３４．７％，对小鼠移植性Ｈ２２肝癌实体瘤的抑制率分别为４２．５％、３５．２％。２００ｍｇ·ｋｇ－１·ｄ－１×１５·ｄ及１００ｍｇ·ｋｇ－１·ｄ－１×１５ｄｉｇ对小鼠移植性ＬＬＣ肺癌实体瘤的抑制率分别为８１．７％、６１．７％，其抑制率均高于Ｇｅ－１３２同剂量组。Ｇｅ－１３２赖氨酸盐对Ｈ２２肝癌腹水型小鼠的生命延长率明显大于ＮＳ组及Ｇｅ－１３２组。提示Ｇｅ－１３２赖氨酸盐的抗癌作用比Ｇｅ－１３２更明显。     

Ge－132赖氨酸盐的抗癌作用

Ｇｅ－１３２赖氨酸盐２００ｍｇ·ｋｇ－１·ｄ－１×１０ｄ及１００ｍｇ·ｋｇ－１·ｄ－１×１０ｄｉｇ对小鼠移植性Ｓ１８０荷瘤实体瘤的抑制率分别为４６．７％、３４．７％，对小鼠移植性Ｈ２２肝癌实体瘤的抑制率分别为４２．５％、３５．２％。２００ｍｇ·ｋｇ－１·ｄ－１×１５·ｄ及１００ｍｇ·ｋｇ－１·ｄ－１×１５ｄｉｇ对小鼠移植性ＬＬＣ肺癌实体瘤的抑制率分别为８１．７％、６１．７％，其抑制率均高于Ｇｅ－１３２同剂量组。Ｇｅ－１３２赖氨酸盐对Ｈ２２肝癌腹水型小鼠的生命延长率明显大于ＮＳ组及Ｇｅ－１３２组。提示Ｇｅ－１３２赖氨酸盐的抗癌作用比Ｇｅ－１３２更明显。     

骆驼蓬硷抗癌作用的研究

研究骆驼蓬硷对小鼠移植性肿瘤体内体外肿瘤细胞的抗癌试验。方法：体内抑瘤实验采用Ｓ－１８０,肝癌Ｈ２２及艾氏腹水癌等荷瘤小鼠、体外实验采用Ｈｅｌａ细胞和Ｓ－１８０细胞。结果：骆驼蓬硷对小鼠移植性肿瘤的抑制率在２８．９４％－４６．７７％之间,对肿瘤细胞的平均生长抑制率在８３．４１％－９２．３０％之间。结论：骆驼蓬硷对肿瘤细胞体内抑瘤试验,体外细胞毒均有明显的抑制作用。     

复方海藻制剂的药效学研究

采用小鼠Ｓ１８０实体瘤及艾氏腹水瘤模型,研究了复方海藻制剂的抗癌及免疫活性,结果发现,复方海藻制剂对小鼠Ｓ１８０肿瘤生长具有明显抑制作用,抑瘤率达３７．９８％～５０．３８％;并能延长腹水型小鼠的生存期,延长率达２１％～５１．３３％;同时促进小鼠脾抗体生成细胞的生成,增加荷瘤小鼠巨噬细胞的吞噬功能。     

鲍鱼多糖的提取及抗肿瘤试验

叙述了鲍鱼多糖的提取方法。研究的结果表明，鲍鱼多糖对Ｓ１８０腹水型小鼠，艾氏腹水型小鼠，肝癌腹水型小鼠有延长寿命的作用，对Ｓ１８０实体型小鼠有明显的抑瘤作用。     

洋苏木素体内外抗肿瘤作用研究

洋苏木素（Ｈａｅｍａｔｏｘｙｌｉｎ）对体外培养的人白血病细胞株Ｋ５６２、ＨＬ—６０及小鼠肥大细胞瘤Ｐ８１５有明显的细胞毒作用。在１００μｇ／ｍｌ浓度下４８小时对靶细胞有９３．８～９６．９％的杀伤作用。对各靶细胞的ＥＤ５０分别是１０．０、８．７５和１２．１２μｇ／ｍｌ。洋苏木素对小鼠移植性白血病Ｐ３８８和Ｌ１２１０有显著的治疗作用，３５０ｍｇ／ｋｇ腹腔给药七天可平均延长小鼠生存时间大于２００％（Ｐ＜０．０１）。对小鼠艾氏腹水癌（Ｅｈｒｌｉｃｈ）也有较强的抑制作用，并表现出明显的效量关系。对实体瘤Ｓ１８０抑制作用不明显。洋苏木素对昆明小鼠的急性ＬＤ５０为６９７．６±７２．６ｍｇ／ｋｇ。     

复方千金藤口服液对实验性肿瘤的治疗及对化疗减毒作用研究

复方千金藤口服液（ＦＱＪＴ）９４０,２０ｇ．ｋｇ＾－１）连续灌胃给药９ｄ,对小鼠移植肉瘤１８０（Ｓ１８０）、子宫肉瘤（Ｕ１４）、肝癌实体型（Ｈｅｐｓ）的肿瘤生长具有明显的抑制作用（Ｐ〈０．０１）,最高抑制率分别为５０．２％,４７．０％,４８．７％,与环磷酰胺（Ｃｙ）相比,虽然作用稍弱,但Ｃｙ有明显降低小鼠体重增长作用,而ＦＱＪＴ则无明显影响。ＦＱＪＴ（４０,２０ｇ．ｋｇ＾－１）连续灌胃给药１０ｄ对     

文蛤多糖抗癌免疫药理作用的研究

小鼠ｉｇ文蛤多糖１００ｍｇ／ｋｇ、２００ｍｇ／ｋｇ,显著抑制Ｓ１８０实体瘤的瘤重显著延长ＥＡＣ腹水瘤荷瘤小鼠的生存时间,对Ｌ１２１０白血病的存活时间无明显影响,以环磷酰胺损伤小鼠的免疫功能,上述剂量的文蛤多糖可使免疫指标显著改善阻挡的增重,白细胞数量增多,在噬能力增强,血清溶血素水平增高,ＳＲ－ＢＣ－ＤＴＨ和ＰＣ－ＤＴＨ反应增强,以上结果表明,文蛤多人有抗癌作用和免疫调节作用。     

基因重组别藻蓝蛋白对小鼠S180肉瘤的抑制作用

研究基因重组别藻蓝蛋白（ＲＡＰＣ）的抑瘤作用,给皮下接种Ｓ１８０瘤细胞的小鼠,每天分别ｉｔ及ｉｐ ＲＡＰＣ１３．４ｍｇ／ｋｇ,６．７ｍｇ／ｋｇ和３．４ｍｇ／ｋｇ,共１０ｄ,第１１ｄ处死小鼠,称瘤重和胸腺重,计数白细胞。结果表明,ＲＡＰＣ对小鼠Ｓ１８０肉瘤有显著抑制作用,瘤重抑制率在４５％～６４％之间,ｉｇ及ｉｐ均有效,对荷瘤小鼠的胸腺指数和白细胞数量无明显影响。     

牛膝多糖的抗肿瘤活性及其免疫增强作用

牛膝多糖（ＡＢＰ）５０ｍｇ·ｋｇ＾－１ｉｐ或２５０ｍｇ·ｋｇ＾－１ｉｇ显抑制小鼠移植性肉瘤Ｓ１８０生长，提高荷瘤小鼠低下的血清ＩｇＧ含量和抗体形成细胞数量及脾淋巴细胞增殖反应。ＡＢＰ ｉｐ还提高荷瘤鼠ＮＫ细胞活性及ＬＰＳ诱生的血清ＴＮＦ－α产生。ＡＢＰ５０－８００μｇ·ｍｌ＾－１体外对Ｓ１８０细胞无直接细胞毒作用，但能增强ＭФ对Ｓ１８０的杀伤作用。提示ＡＢＰ抗肿瘤作用与其增强宿主免疫功能有关。     

聚酯型儿茶素对小鼠的免疫调节作用

目的 :研究聚酯型儿茶素对正常小鼠及实验性免疫功能低下小鼠的影响。方法 :选用二硝基氟苯 (DNFB)致小鼠迟发型超敏反应 (DTH)模型、小鼠腹腔巨噬细胞 (PMΦ )吞噬鸡红细胞模型以及血清溶血素生成法 (HC50) ,分别观察对正常小鼠特异性细胞免疫、非特异性细胞免疫和体液免疫功能的影响。以环磷酰胺腹腔注射15mg/(kg·d)×4d造成免疫功能低下的模型 ,采用DTH、碳粒廓清法及血清溶血素生成法测定有关免疫指标。结果 :聚酯型儿茶素 (50、100、200mg/(kg·d)×7d)可明显增强正常小鼠的耳片肿胀度、腹腔巨噬细胞吞噬功能及血清溶血素生成 ,明显提高环磷酰胺所致免疫低下小鼠的DTH反应、碳粒廓清K和α值及HC50 值。结论 :聚酯型儿茶素对正常及免疫功能低下小鼠的细胞免疫和体液免疫均具有增强作用。     

Antitumor activity of Klebsiella 03 lipopolysaccharide in mice

The antitumor activity of Klebsiella 03 lipopolysaccharide (KO3 LPS) isolated from the culture supernatant against S180 sarcoma, Ehrlich carcinoma, MM2 mammary carcinoma and Meth A fibrosarcoma in mice was investigated. KO3 LPS significantly prolonged the lifespan of S180-bearing ddY mice and MM2-bearing C3H/He mice by intraperitoneal pre- or postmedication at doses ranging from 0.1 to 1.0 mg/kg. The LPS also inhibited the growth of subcutaneously inoculated Ehrlich carcinoma in ddY mice and Meth A sarcoma in BALB/c mice by intraperitoneal, intravenous or intratumoral administration. The intratumoral injection of KO3 LPS was most effective and results by the intravenous and the intraperitoneal administrations followed in effectiveness, but the administration through the subcutaneous route was hardly effective. Thus, KO3 LPS was shown to have antitumor activity on both allogeneic tumors and syngeneic tumors. It was also indicated in this study that the lifeprolonging effect of KO3 LPS on S180 ascites type tumor-bearing mice was significantly minimized by pretreatment of cyclophosphamide and that the LPS did not influence the cell viability of HeLa cells, Ehrlich cells and MM2 cells in vitro. These results suggest that the antitumor activity of KO3 LPS is provided by host-mediated actions.     

青蒿素抗肿瘤作用的初步研究

目的 研究菊科植物黄花蒿的单体青蒿素(Artimisinine, ART)的抗肿瘤作用.方法 采用动物移植性肿瘤实验方法.结果 50 mg·kg-1 ART对小鼠移植性肉瘤S180的抑瘤率为37.88%;100 mg·kg-1 ART对小鼠移植性肝癌Heps实体瘤的抑瘤率为35.23%;ART对艾氏腹水癌EAC小鼠生命延长率无统计学意义.结论 ART具有良好的抗肿瘤作用.     

螺旋藻多糖硫酸酯化修饰前后抗肿瘤及免疫活性的研究

比较螺旋藻多糖硫酸酯化修饰前后体内外抗肿瘤及免疫活性.采用MTT比色法研究药物在体外对人肿瘤细胞株的抑制作用,促进正常小鼠脾淋巴细胞的增殖活性以及对荷瘤小鼠NK和CTL细胞活性的影响.采用移植性肿瘤实验方法考察了药物对小鼠S180肉瘤的抑制作用.结果显示,螺旋藻多糖（NPSP）对肿瘤细胞株几乎无细胞毒作用,硫酸酯化螺旋藻多糖（SNPSP）对肿瘤细胞株具有显著的细胞毒作用,其中对SMMC-7721人肝癌细胞株抑制率最高达50%.NPSP50 mg/kg对小鼠S180肉瘤无抑制,相同剂量的SNPSP对小鼠S180肉瘤的抑制率达35.42%.NPSP具有促进脾淋巴细胞增殖作用,但对ConA和LPS诱导的脾淋巴细胞增殖反应无促进作用,SNPSP促进脾淋巴细胞增殖作用较NPSP增强,同时对ConA和LPS诱导的脾淋巴细胞增殖反应也具有明显的促进作用.NPSP和SNPSP均能促进荷瘤小鼠NK细胞和CTL细胞活性,其中SNPSP促进CTL细胞活性较NPSP增强.     

Pharmacological study on kefir--a fermented milk product in Caucasus. I. On antitumor activity (1)]

The antitumor activity of kefir (YK-1), a fermented milk product in Caucasus, was investigated. YK-1 at oral doses of 100 or 500 mg/kg inhibited the proliferation of solid tumor of Ehrlich ascites carcinoma transplanted subcutaneously in mice. YK-1 did not show an inhibitory effect on the ear swelling induced contact dermatitis caused by picryl chloride (PC-CD). However, YK-1 inhibited the immunosuppression in Ehrlich carcinoma-bearing mice and with the frozen and dried ascites of the tumor-bearing mice containing immunosuppressive substances (EC-sup) in PC-CD-induced mice. And also, YK-1 activated the immunosuppressive activity of spleen cells of mouse treated with EC-sup. These results suggest that YK-1 may have antitumor activity against Ehrlich carcinoma and activate the immunosuppression with it.     

1,2∶5,6-二去水卫矛醇与美登新联合用药的实验研究

去水卫矛醇(DAG)和美登新(MAY)同时或间隔24小时给药(无论先后顺序如何),对EAC小鼠的生命延长和杀瘤细胞均有协同作用;不同先后顺序给药对L1210得到同样结果,但同时给药则无协同作用。给DAG后24小时再给MAY,对接种21天的B16黑色素瘤瘤重抑制率超过了“相加作用”,但不能延长生命。联合用药对HepA及S180无协同疗效。联合使用DAG及MAY,对正常小鼠骨髓干细胞的杀灭低千“相加作用”。本实验表明,DAG及MAY间隔24小时给药较同时给药为佳。     

The anticancer activities of wogonin in murine sarcoma S180 both in vitro and in vivo

The anticancer effects of wogonin on murine sarcoma S180 both in vitro and in vivo were investigated, and its pro-apoptotic molecular mechanism was further studied. Wogonin treatment resulted in significant inhibition of S180 cells in a concentration-dependent manner detected by MTT assay. The IC(50) value for 48 h was (7.37+/-1.53)x10(-5) M. Typical morphological changes and apoptosis bleb phenomenon in S180 cells exposed to wogonin were distinctly observed by the inverted light microscope and the fluorescence microscope, respectively. According to protocols of transplanted tumor research,(1)) mice were transplanted with tumor cells S180. The weight of tumor and the peripheral leucocyte count were observed after the treatment of wogonin. The significant suppression of tumor growth was observed, and the peripheral leucocyte count of S180-bearing mice remained no significant changes compared with control group. After the treatment of 40 mg/kg wogonin, the inhibitory rate of tumor weight was 53.01%. Additional DNA fragmentation assay showed that wogonin induced apoptosis on murine sarcoma S180 tissue. RT-PCR results indicated that the increasing mRNA levels of bax and p53 and the decreasing mRNA level of bcl-2 were induced by wogonin. Western-blot assay showed that the increasing protein level of bax and the decreasing protein level of bcl-2 were induced by wogonin. Collectively, wogonin could induce apoptosis in murine sarcoma S180 thereby inhibiting the tumor growth both in vitro and in vivo. The pro-apoptotic effects might be related to the improvement of mRNA level of p53, the improvement of mRNA and protein levels of bax, and the reduction of mRNA and protein levels of bcl-2.     

2,3-吲哚醌抗肿瘤作用研究

目的研究2,3-吲哚醌的抗肿瘤作用及机制。方法观察2,3-吲哚醌对小鼠肉瘤(S180)、小鼠肝癌(H22)、小鼠艾氏腹水瘤实体型(EC)和腹水型(EAC)的抗肿瘤作用;免疫组化法测定药物对S180肿瘤组织中PCNA、Bcl-2蛋白表达的影响;核染色法(Hoechst33258染色)和MTT法观察2,3-吲哚醌对人神经母瘤(SH-SY5Y)细胞体外促凋亡和抗增殖作用。结果2,3-吲哚醌对3种小鼠移植性肿瘤有抑制作用,对荷瘤鼠血液白细胞水平无影响;2,3-吲哚醌可抑制S180肿瘤组织中PCNA,Bcl-2蛋白表达;2,3-吲哚醌可促进人神经母瘤细胞凋亡并抑制其增殖。结论2,3-吲哚醌有抗肿瘤作用,其机制与抑制肿瘤细胞增殖,诱导肿瘤细胞凋亡有关。     

洋葱挥发油抗肿瘤作用的实验研究

目的:研究洋葱挥发油离体对人肝癌细胞株(QCY-7703)、人胃癌细胞株(MGC-803)、人宫颈癌细胞株(Hela)、人肺腺癌细胞株(SPC-A-1)的增殖抑制作用和在体对小鼠肉瘤S180及小鼠艾氏腹水癌的抑制作用。方法:采用MTT法检测不同剂量的洋葱挥发油对4种肿瘤细胞增殖的影响,复制小鼠肉瘤S180和小鼠艾氏腹水癌模型,检测不同剂量(1000、500、250mg·kg-1)的洋葱挥发油对小鼠肿瘤的抑制作用,比较其抑制率。结果:洋葱挥发油离体和在体实验中对肿瘤细胞均具有较强的增殖抑制作用。结论:洋葱挥发油能抑制多种肿瘤细胞的增殖,对肿瘤细胞有明显的细胞毒性作用。     

肝康灵胶囊对化疗药物的增效减毒作用研究

目的:研究肝康灵胶囊(GKL)对化疗药物的增效减毒作用。方法:以体内抑瘤实验测定GKL单独使用及与顺铂(DDP)伍用时对小鼠移植性肿瘤(S180肉瘤、H22肝癌和Lewis肺癌肿瘤)的抑癌率,并考察GKL分别与DDP和丝裂霉素(MMC)伍用时对荷瘤小鼠白细胞数、免疫器官质量、巨噬细胞吞噬功能和自然杀伤(NK)细胞活性的影响。结果:GKL对小鼠移植性肿瘤(S180肉瘤、H22肝癌和Lewis肺癌肿瘤)均有抑制作用;当其分别与MMC和DDP伍用时,能显著提高2种化疗药物的抑癌率(P<0.01);GKL还可显著减少MMC和DDP所致小鼠白细胞下降、免疫器官萎缩、巨噬细胞吞噬功能下降和NK细胞活性降低等副作用。结论:GKL具有显著的抗肿瘤和增效减毒作用。