电针配合神经生长因子对脑缺血大鼠学习记忆能力的影响

Objective To observe the effect of nerve growth factor(NGF)and electro-acupuncture(EA) on learning and memory in rats with cerebral ischemia.Methods An experimental model of cerebral ischemiareperfusion was established in 40 rats,who were then randomly divided into a model group,a sham operated group,a NGF group,an acupuncture group and a NGF plus acupuncture group.Three weeks were allowed for the recovery of the blood-brain barrier lesion caused by ischemia-reperfusion before intravenous injection of NGF(10μg/kg,once daily for 15 days)via the vena caudalis.In the two acupuncture groups,this was administered in combination with EA at the Baihui(DU20)and Yamen(DU15)acupoints(frequency 100 Hz,2 mA,20 min/time,once daily for 15 days).Morris' water maze test was used to assess any changes in spatial learning and memory abilities.Results The ischemic rats showed significant learning and memory disorders compared with the sham-operated group.In the NGF group,the rats' learning and memory abilities did not impmve significantly compared with the model group and the EA group.Significantly better learning was observed in the NGF+EA group compared with the model group,the EA group and the NGF group.Conclusion NGF combined with EA can improve the learning and memory abilities of rats with learning and memory dysfunction caused by cerebral ischemia-repedusion.     

电针配合神经生长因子对脑缺血大鼠学习记忆能力的影响

Objective To observe the effect of nerve growth factor(NGF)and electro-acupuncture(EA) on learning and memory in rats with cerebral ischemia.Methods An experimental model of cerebral ischemiareperfusion was established in 40 rats,who were then randomly divided into a model group,a sham operated group,a NGF group,an acupuncture group and a NGF plus acupuncture group.Three weeks were allowed for the recovery of the blood-brain barrier lesion caused by ischemia-reperfusion before intravenous injection of NGF(10μg/kg,once daily for 15 days)via the vena caudalis.In the two acupuncture groups,this was administered in combination with EA at the Baihui(DU20)and Yamen(DU15)acupoints(frequency 100 Hz,2 mA,20 min/time,once daily for 15 days).Morris' water maze test was used to assess any changes in spatial learning and memory abilities.Results The ischemic rats showed significant learning and memory disorders compared with the sham-operated group.In the NGF group,the rats' learning and memory abilities did not impmve significantly compared with the model group and the EA group.Significantly better learning was observed in the NGF+EA group compared with the model group,the EA group and the NGF group.Conclusion NGF combined with EA can improve the learning and memory abilities of rats with learning and memory dysfunction caused by cerebral ischemia-repedusion.     

电针配合神经生长因子对脑缺血大鼠学习记忆能力的影响

Objective To observe the effect of nerve growth factor(NGF)and electro-acupuncture(EA) on learning and memory in rats with cerebral ischemia.Methods An experimental model of cerebral ischemiareperfusion was established in 40 rats,who were then randomly divided into a model group,a sham operated group,a NGF group,an acupuncture group and a NGF plus acupuncture group.Three weeks were allowed for the recovery of the blood-brain barrier lesion caused by ischemia-reperfusion before intravenous injection of NGF(10μg/kg,once daily for 15 days)via the vena caudalis.In the two acupuncture groups,this was administered in combination with EA at the Baihui(DU20)and Yamen(DU15)acupoints(frequency 100 Hz,2 mA,20 min/time,once daily for 15 days).Morris' water maze test was used to assess any changes in spatial learning and memory abilities.Results The ischemic rats showed significant learning and memory disorders compared with the sham-operated group.In the NGF group,the rats' learning and memory abilities did not impmve significantly compared with the model group and the EA group.Significantly better learning was observed in the NGF+EA group compared with the model group,the EA group and the NGF group.Conclusion NGF combined with EA can improve the learning and memory abilities of rats with learning and memory dysfunction caused by cerebral ischemia-repedusion.     

电针配合神经生长因子对脑缺血大鼠学习记忆能力的影响

Objective To observe the effect of nerve growth factor(NGF)and electro-acupuncture(EA) on learning and memory in rats with cerebral ischemia.Methods An experimental model of cerebral ischemiareperfusion was established in 40 rats,who were then randomly divided into a model group,a sham operated group,a NGF group,an acupuncture group and a NGF plus acupuncture group.Three weeks were allowed for the recovery of the blood-brain barrier lesion caused by ischemia-reperfusion before intravenous injection of NGF(10μg/kg,once daily for 15 days)via the vena caudalis.In the two acupuncture groups,this was administered in combination with EA at the Baihui(DU20)and Yamen(DU15)acupoints(frequency 100 Hz,2 mA,20 min/time,once daily for 15 days).Morris' water maze test was used to assess any changes in spatial learning and memory abilities.Results The ischemic rats showed significant learning and memory disorders compared with the sham-operated group.In the NGF group,the rats' learning and memory abilities did not impmve significantly compared with the model group and the EA group.Significantly better learning was observed in the NGF+EA group compared with the model group,the EA group and the NGF group.Conclusion NGF combined with EA can improve the learning and memory abilities of rats with learning and memory dysfunction caused by cerebral ischemia-repedusion.     

电针配合神经生长因子对脑缺血大鼠学习记忆能力的影响

Objective To observe the effect of nerve growth factor(NGF)and electro-acupuncture(EA) on learning and memory in rats with cerebral ischemia.Methods An experimental model of cerebral ischemiareperfusion was established in 40 rats,who were then randomly divided into a model group,a sham operated group,a NGF group,an acupuncture group and a NGF plus acupuncture group.Three weeks were allowed for the recovery of the blood-brain barrier lesion caused by ischemia-reperfusion before intravenous injection of NGF(10μg/kg,once daily for 15 days)via the vena caudalis.In the two acupuncture groups,this was administered in combination with EA at the Baihui(DU20)and Yamen(DU15)acupoints(frequency 100 Hz,2 mA,20 min/time,once daily for 15 days).Morris' water maze test was used to assess any changes in spatial learning and memory abilities.Results The ischemic rats showed significant learning and memory disorders compared with the sham-operated group.In the NGF group,the rats' learning and memory abilities did not impmve significantly compared with the model group and the EA group.Significantly better learning was observed in the NGF+EA group compared with the model group,the EA group and the NGF group.Conclusion NGF combined with EA can improve the learning and memory abilities of rats with learning and memory dysfunction caused by cerebral ischemia-repedusion.     

电针配合神经生长因子对脑缺血大鼠学习记忆能力的影响

Objective To observe the effect of nerve growth factor(NGF)and electro-acupuncture(EA) on learning and memory in rats with cerebral ischemia.Methods An experimental model of cerebral ischemiareperfusion was established in 40 rats,who were then randomly divided into a model group,a sham operated group,a NGF group,an acupuncture group and a NGF plus acupuncture group.Three weeks were allowed for the recovery of the blood-brain barrier lesion caused by ischemia-reperfusion before intravenous injection of NGF(10μg/kg,once daily for 15 days)via the vena caudalis.In the two acupuncture groups,this was administered in combination with EA at the Baihui(DU20)and Yamen(DU15)acupoints(frequency 100 Hz,2 mA,20 min/time,once daily for 15 days).Morris' water maze test was used to assess any changes in spatial learning and memory abilities.Results The ischemic rats showed significant learning and memory disorders compared with the sham-operated group.In the NGF group,the rats' learning and memory abilities did not impmve significantly compared with the model group and the EA group.Significantly better learning was observed in the NGF+EA group compared with the model group,the EA group and the NGF group.Conclusion NGF combined with EA can improve the learning and memory abilities of rats with learning and memory dysfunction caused by cerebral ischemia-repedusion.     

电针配合神经生长因子对脑缺血大鼠学习记忆能力的影响

Objective To observe the effect of nerve growth factor(NGF)and electro-acupuncture(EA) on learning and memory in rats with cerebral ischemia.Methods An experimental model of cerebral ischemiareperfusion was established in 40 rats,who were then randomly divided into a model group,a sham operated group,a NGF group,an acupuncture group and a NGF plus acupuncture group.Three weeks were allowed for the recovery of the blood-brain barrier lesion caused by ischemia-reperfusion before intravenous injection of NGF(10μg/kg,once daily for 15 days)via the vena caudalis.In the two acupuncture groups,this was administered in combination with EA at the Baihui(DU20)and Yamen(DU15)acupoints(frequency 100 Hz,2 mA,20 min/time,once daily for 15 days).Morris' water maze test was used to assess any changes in spatial learning and memory abilities.Results The ischemic rats showed significant learning and memory disorders compared with the sham-operated group.In the NGF group,the rats' learning and memory abilities did not impmve significantly compared with the model group and the EA group.Significantly better learning was observed in the NGF+EA group compared with the model group,the EA group and the NGF group.Conclusion NGF combined with EA can improve the learning and memory abilities of rats with learning and memory dysfunction caused by cerebral ischemia-repedusion.     

电针配合神经生长因子对脑缺血大鼠学习记忆能力的影响

Objective To observe the effect of nerve growth factor(NGF)and electro-acupuncture(EA) on learning and memory in rats with cerebral ischemia.Methods An experimental model of cerebral ischemiareperfusion was established in 40 rats,who were then randomly divided into a model group,a sham operated group,a NGF group,an acupuncture group and a NGF plus acupuncture group.Three weeks were allowed for the recovery of the blood-brain barrier lesion caused by ischemia-reperfusion before intravenous injection of NGF(10μg/kg,once daily for 15 days)via the vena caudalis.In the two acupuncture groups,this was administered in combination with EA at the Baihui(DU20)and Yamen(DU15)acupoints(frequency 100 Hz,2 mA,20 min/time,once daily for 15 days).Morris' water maze test was used to assess any changes in spatial learning and memory abilities.Results The ischemic rats showed significant learning and memory disorders compared with the sham-operated group.In the NGF group,the rats' learning and memory abilities did not impmve significantly compared with the model group and the EA group.Significantly better learning was observed in the NGF+EA group compared with the model group,the EA group and the NGF group.Conclusion NGF combined with EA can improve the learning and memory abilities of rats with learning and memory dysfunction caused by cerebral ischemia-repedusion.     

电针配合神经生长因子对脑缺血大鼠学习记忆能力的影响

Objective To observe the effect of nerve growth factor(NGF)and electro-acupuncture(EA) on learning and memory in rats with cerebral ischemia.Methods An experimental model of cerebral ischemiareperfusion was established in 40 rats,who were then randomly divided into a model group,a sham operated group,a NGF group,an acupuncture group and a NGF plus acupuncture group.Three weeks were allowed for the recovery of the blood-brain barrier lesion caused by ischemia-reperfusion before intravenous injection of NGF(10μg/kg,once daily for 15 days)via the vena caudalis.In the two acupuncture groups,this was administered in combination with EA at the Baihui(DU20)and Yamen(DU15)acupoints(frequency 100 Hz,2 mA,20 min/time,once daily for 15 days).Morris' water maze test was used to assess any changes in spatial learning and memory abilities.Results The ischemic rats showed significant learning and memory disorders compared with the sham-operated group.In the NGF group,the rats' learning and memory abilities did not impmve significantly compared with the model group and the EA group.Significantly better learning was observed in the NGF+EA group compared with the model group,the EA group and the NGF group.Conclusion NGF combined with EA can improve the learning and memory abilities of rats with learning and memory dysfunction caused by cerebral ischemia-repedusion.     

Effects of Electroacupuncture Combined with Repetitive Transcranial Magnetic Stimulation on the Expression of Nestin in Neural Stem Cell after Focal Cerebral Ischemia in Adult Rats*

Objective: To investigate the influence of electroacupuncture (EA) combined with repetitive transcranial magnetic stimulation(rTMS) on the temporal profile of nestin expression after induction of focal cerebral ischemia in adult rats and to explore the mechanism of EA combined with rTMS in treating ischemic brain injury. Method: The model of transient focal ischemia was produced by occlusion of middle cerebral artery. Seventy-five Wistar rats were randomly divided into normal group, model group, EA group, rTMS group and EA+rTMS group. The neurologic impairment rating and ability of learning and memory were observed at the 7th、14th and 28th d after infarction respectively. Meanwhile, Western blotting was used to observe the number of nestin expression positive cells. Result: Nestin-positive cells were found in cortex, subgranular zone(SGZ), subventricular zone (SVZ) of the ipsilateral side at different time points after cerebral ischemia. The number of nestin-positive cells peaked at the 7th d, began to decrease at the 14th d and was significantly higher in EA+rTMS group than that in model group(P<0.05), then almost reached normal at the 28th d. The improvement of neural motor function deficits as well as the indexes of learning and memory were more obvious in EA+rTMS group compared with model group(P<0.01, P<0.05). These effects were most obvious in EA+rTMS group compared with the EA and rTMS group(P<0.05). Conclusion: EA and rTMS possess the potency of building up and can increase the number of nestin-positive cells in some brain regions after focal cerebral ischemia, which might be one of the important mechanisms of EA combined with rTMS in treating ischemia brain injury.     

Use of crystepin in combination with obsidan in patients with diabetes mellitus with arterial hypertension

Examination included 70 patients with diabetes mellitus in combination with arterial hypertension of different origin (II stage essential hypertension and symptomatic renal arterial hypertension). Crystepin (2-3 tablets per 24 h) in combination with beta-adrenoblocker obsidan (40-80 mg/24 h) was used for treatment. Basic hemodynamic parameters and the state of the renin-aldosterone system were determined. The hemodynamic hypotensive effects in these patients due to the influence of the above therapy are uniform and depend on the form of attendant arterial hypertension. The hypotensive effect of crystepin used in combination with obsidan was more pronounced in patients with diabetes and II stage essential hypertension than that in those with diabetes and renal hypertension. The concentration of aldosterone and renin activity of blood plasma in patients with diabetes and arterial hypertension during treatment with crystepin and obsidan had no regular connection with the hemodynamic parameters.     

硝酸甘油联合托拉塞米在高血压危象并发急性肺水肿患者的应用

目的研究硝酸甘油联合托拉塞米治疗高血压危象合并急性肺水肿的效果。方法选取我院收治的高血压危象合并急性肺水肿患者80例作为研究对象。随机分为对照组40例,采用托拉塞米治疗;试验组40例,采用硝酸甘油联合托拉塞米治疗。比较两组患者治疗效果、临床指标改善程度及并发症发生率。结果治疗后对照组患者血压、呼吸频率及氧分压改善程度均低于试验组,且治疗有效率(77.5%)低于试验组(92.5%),差异显著(P0.05);对照组患者并发症发生率(20.0%)高于试验组(7.5%),差异显著(P0.05)。结论采用硝酸甘油联合托拉塞米在治疗高血压危象合并急性肺水肿安全性高,可减少并发症,改善治疗效果,提高患者生活质量。     

Satisfaction with upper-extremity surgery in individuals with tetraplegia

OBJECTIVE: To measure the satisfaction of individuals with tetraplegia with their upper-extremity reconstructive surgery. DESIGN: Survey. SETTING: Two Spinal Cord Injury Model Systems centers. PARTICIPANTS: Sixty-seven individuals with spinal cord injury at the C4 through C8 motor level (107 arms). INTERVENTIONS: Participants had upper-extremity surgery to improve function. The surgical procedures included tendon transfers for elbow extension, wrist extension, hand grasp, and pinch or hand grasp neuroprosthesis. MAIN OUTCOME MEASURE: A survey was mailed to participants, who were asked to respond to statements such as, "If I had it to do over, I would have the hand/arm surgery again," using a 5-level Likert scale (ranging from strongly agree to strongly disagree). RESULTS: Seventy percent of the participants were generally satisfied with the results of their upper-extremity surgery, 77% reported a positive impact on their lives, 68% reported improvements in activities of daily living (ADLs), 66% reported improved independence, 69% reported improvement in occupation, 71% reported improved appearance or neutral, 78% reported their hand worked as well (or neutral) as it did when surgery was first performed, and 86% reported postoperative therapy as being beneficial. CONCLUSIONS: Upper-extremity surgery had a positive impact on life, increased ability to perform ADLs and to be independent, and improved quality of life.     

Observations with Rabenid in patients with locomotor disease

By examining 139 patients suffering from locomotor diseases the author could observe the characteristic accompanying diseases as well as high uric acid level in 40 cases. She analyses the current opinions on hyperuricaemia and reports on her observations with uricosuric and antithrombotic coated Rabenid tablet (RG). She found sulfinpyrazone to be effective and valuable in the examined indication field.     

休克状态下PSVT多种药物转复成功1例

1 临床资料 患者女，77岁，主因心慌、乏力、大汗六小时就诊。查体，急性面容，血压波动在70～86/37～54mmHg；心率：160次/分；呼吸：26次/分；口唇紫绀，双肺呼吸音清；心率齐，心电图示阵发性室上心动过度，无预激综合症和房颤；血生化示电解质正常。     

Changes in myocardial function in patients with hypertension during treatment with minipress

Altogether 33 patients with arterial hypertension were investigated to study changes of left ventricular systolic phasic analysis, caused by prazosin therapy. Two groups were identified on the basis of these changes: indices in the 1st group (intrasystolic index, myocardial tension index, Blumberger coefficient) improved, in the 2nd group they deteriorated. The antihypertensive effect of prazosin should be assessed not only by the level of BP reduction but also by changes of left ventricular systolic phasic analysis. In the deterioration of indices of left ventricular systolic phasic analysis during a test with single administration of prazosin, antihypertensive therapy should be reconsidered.