纵隔棘球蚴囊肿一例

病例资料　患者 ,女 ,5 2岁 ,农民 ,云南省富民县人 ,未到过疫区。发现左纵隔占位性病变 1年。胸前不适伴轻度呼吸困难。血常规 :白细胞 4.2×10 9/l,中性粒细胞 0 .5 7,淋巴细胞 0 .3 7,单核细胞 0 .0 6。CT平扫示左上中纵隔紧邻主动脉弓一椭圆形囊性病灶 ,边缘光滑、整齐 ,内     

子宫合并中腹棘球蚴囊肿1例

患者,女,48岁.月经史：14Y（3-5D）/（28-30）,末次月经：2006-07.自述自2005年起月经开始不规律,2～3个月至半年来潮一次,无腹痛及其他不适.妊娠分娩史：妊2娩2流0.2006-08-12因进食油腻,食后突然出现右上腹胀痛不适,伴恶心、呕吐,呕吐物为胃内容物,不向他处放射,无腹泻等其他特殊不适,于2006-09-05来我院就诊.妇科情况：外阴发育正常,已婚已产式,阴道前后壁轻度彭出.于右侧附件区可触及一约12 cm×10 cm×7 cm大小包块,表面光滑、质中、活动差、压痛（＋）,无反跳痛.     

右侧输卵管巨大棘球蚴囊肿1例

患者,女性,５４岁,哈族。自述４年前无意中触摸腹部时触及两个拳头大小的包块,伴腹胀,腹痛,未作检查及治疗,近日包块渐大且腹胀、腹痛加重。患者有密切犬羊接触史。查体：右下腹及下腹部可触及１０ｃｍ×１０ｃｍ及６ｃｍ×６ｃｍ包块,光滑、质中,边界清楚,活动...     

肝棘球蚴囊肿致梗阻性黄疸18例

梗阻性黄疸常见的病因为胆总管结石、胆道炎性狭窄、胆道肿瘤、手术损伤、先天性胆道畸形和胰头占位等。在农牧区易见到肝棘球蚴囊肿为多发病,肝棘球蚴囊肿破人胆道系统也是一种梗阻性黄疸的病因。肝棘球蚴囊肿破裂是棘球蚴病的常见并发症,但破入胆道系统致梗阻性黄疸则是较少见的并发症。现将我院1995～2008年收治肝棘球蚴867例中棘球蚴囊肿破入胆道的18例,作一报道及分析。     

肝门区肝棘球蚴囊肿切除39例

累及肝Ⅰ、Ⅳ、Ⅴ、Ⅶ、Ⅷ区段内的肝棘球蚴囊肿称为肝门区棘球蚴囊肿，因其解剖关系复杂。手术难度大。并发症多。难以手术切除。我院自1995—07～2005—07行肝单房性棘球蚴囊肿完整切除66例，其中肝门区肝单房性棘球蚴囊肿完整切除31例，部分切除8例，取得了较好的效果。现报告如下。     

肝棘球蚴囊肿自发性破裂3例治疗体会

我科从１９９６年１月～１９９８年１１月共收治肝棘球蚴囊肿３１例,其中自发性破裂３例,现报告如下。例１,女性,２７岁,汉族。因进行性腹部隆起伴腹胀２月而收住。经Ｂ超检查提示肝巨大囊性包块,卡松尼试验强阳性。查体：腹部膨隆,右上腹部肋下可触及２５ｃｍ×２...     

肝、肺及肌肉多发感染棘球蚴囊肿1例

患者 ,女 ,2 7岁 ,哈萨克族 ,牧民。因左股内侧肿块 6月、右胸部隐痛伴咳嗽 4月加重 3d入院。右胸部呈持续性隐痛 ,向右肩背放射 ,咳嗽呈间断性 ,活动及夜间加重。在当地诊所抗炎治疗症状可缓解 ,近日上述症状加重伴发热、咳脓痰。查体 :体温 38.1℃ ,脉搏 1 2 0·min-1 。右肺呼吸动度弱 ,语颤增强 ,右中下肺呼吸音较弱 ,未闻及明显干湿性罗音 ,无胸膜摩擦音。肝上界位于右锁骨中线第 4肋间 ,肝区轻度叩痛。左股内侧可触及 1 0cm× 8cm包块 ,质中、边界清楚光滑、可活动、基底宽、轻压痛。胸片示右肺野有一约 1 3.0cm× 1 1 .5cm圆形阴影、…     

肝、肺及肌肉多发感染棘球蚴囊肿1例

患者 ,女 ,2 7岁 ,哈萨克族 ,牧民。因左股内侧肿块 6月、右胸部隐痛伴咳嗽 4月加重 3ｄ入院。右胸部呈持续性隐痛 ,向右肩背放射 ,咳嗽呈间断性 ,活动及夜间加重。在当地诊所抗炎治疗症状可缓解 ,近日上述症状加重伴发热、咳脓痰。查体 :体温 38.1℃ ,脉搏 12 0·ｍｉｎ-1。右肺呼吸动度弱 ,语颤增强 ,右中下肺呼吸音较弱 ,未闻及明显干湿性罗音 ,无胸膜摩擦音。肝上界位于右锁骨中线第 4肋间 ,肝区轻度叩痛。左股内侧可触及 10ｃｍ× 8ｃｍ包块 ,质中、边界清楚光滑、可活动、基底宽、轻压痛。胸片示右肺野有一约 13.0ｃｍ× 11.5ｃｍ圆形…     

肝棘球蚴囊肿破入胆道七例误诊原因分析

肝棘球蚴囊肿破入胆道为肝棘球蝴病的严重并发症之一，本文对我院1984～1994年收治的7例误诊病例进行分析，从中吸取经验教训。     

电视腹腔镜摘除肺棘球蚴囊肿二例护理体会

肺棘球蝴病的传统手木方法是剖胸将内囊取出。1993年7月和1994年1月我科结合肝棘球蝴包囊穿刺治疗方法，运用电视腹腔镜，做了2例肺棘     

Rapid and sensitive identification of human blood by an ELISA-ABC method using a biotinylated antibody against human HbA0

Summary A direct enzyme-linked immunosorbent assay (ELISA) using an avidin-biotin complex (ABC) system for the identification of human blood is described. In this ELISA-ABC method, in which biotin-labeled goat IgG antibody against human HbA₀ was used, it was possible clearly to distinguish human blood from the blood of other species, including that of Japanese monkeys. It took about 3 h to obtain the results. Human Hb concentrations ranging from 22 ng to 169 g produced a positive reaction, and the minimum detection limit in terms of the highest possible dilution of human blood was 1:640,000.     

Early immunoscintigraphic localisation of a mediastinal tumour with indium 111-DTPA CEA-specific F(ab')2 monoclonal antibody fragments (BW 431/31) using second tracer isocontour technique

A female patient with steadily increasing carcinoembryonic antigen (CEA) serum levels of unknown origin was referred for immunoscintigraphy with indium 111-labelled CEA-specific monoclonal antibody. The procedure revealed a tumour, undetectable by conventional diagnostic methods. Anatomical landmarking using the second tracer isocontour technique allowed the distinction between an intra- or extrapulmonary lesion. Two months later, tumour infiltration along the aortic arch was confirmed by a targeted angio-CT scan. Upon surgery, the diagnosis was definitely established histologically (undifferentiated, solid large cell carcinoma, most probably arising from the bronchus), and staining by CEA-specific immunohistochemistry confirmed the presence of the CEA antigen.     

New detection method for ribonuclease 2 (RNase 2) using immunoblotting with specific antibody

Summary A ribonuclease (RNase) was isolated from the urine of a 35-year-old male and purified to electrophoretic homogeneity. The enzyme was tentatively designated RNase 2. A rabbit antibody produced by injection of the purified RNase 2 was able to distinguish RNase 2 from another type of RNase coexisting in body fluids. With this antibody it was possible to detect RNase 2 isozymes in human serum and urine without difficulty using isoelectric focusing or sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by immunoblotting. Both RNase 2 in serum and urine seemed to exist in multiple forms with regard to their molecular masses and pI values. This technique may prove to be useful in genetic and forensic studies of RNase polymorphism.Supported in part by grants from the Japan Brain Foundation and Tokyo Immunopharmacology Institute, and a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan     

Development of a SNP set for human identification: A set with high powers of discrimination which yields high genetic information from naturally degraded DNA samples in the Thai population

This study describes the development of a SNP typing system for human identification in the Thai population, in particular for extremely degraded DNA samples. A highly informative SNP marker set for forensic identification was identified, and a multiplex PCR-based Invader assay was developed. Fifty-one highly informative autosomal SNP markers and three sex determination SNP markers were amplified in two multiplex PCR reactions and then detected using Invader assay reactions. The average PCR product size was 71 base pairs. The match probability of the 54-SNP marker set in 124 Thai individuals was 1.48 × 10⁻²¹, higher than that of STR typing, suggesting that this 54-SNP marker set is beneficial for forensic identification in the Thai population. The selected SNP marker set was also evaluated in 90 artificially degraded samples, and in 128 naturally degraded DNA samples from real forensic casework which had shown no profiles or incomplete profiles when examined using a commercial STR typing system. A total of 56 degraded samples (44%) achieved the matching probability (PM) equivalent to STR gold standard analysis (successful genotyping of 44 SNP markers) for human identification. These data indicated that our novel 54-SNP marker set provides a very useful and valuable approach for forensic identification in the Thai population, especially in the case of highly to extremely degraded DNA.In summary, we have developed a set of 54 Thai-specific SNPs for human identification which have higher discrimination power than STR genotyping. The PCRs for these 54 SNP markers were successfully combined into two multiplex reactions and detected with an Invader assay. This novel SNP genotyping system also yields high levels of genetic information from naturally degraded samples, even though there are much more difficult to recover than artificially degraded samples.     

Design and development of a prototype endocavitary probe for high-intensity focused ultrasound delivery with integrated magnetic resonance imaging

Purpose

To integrate a high intensity focused ultrasound (HIFU) transducer with an MR receiver coil for endocavitary MR‐guided thermal ablation of localized pelvic lesions.

Materials and Methods

A hollow semicylindrical probe (diameter 3.2 cm) with a rectangular upper surface (7.2 cm × 3.2 cm) was designed to house a HIFU transducer and enable acoustic contact with an intraluminal wall. The probe was distally rounded to ease endocavitary insertion and was proximally tapered to a 1.5‐cm diameter cylindrical handle through which the irrigation tubes (for transducer cooling) and electrical connections were passed. MR compatibility of piezoceramic and piezocomposite transducers was assessed using gradient‐echo (GRE) sequences. The radiofrequency (RF) tuning of identical 6.5 cm × 2.5 cm rectangular receiver coils on the upper surface of the probe was adjusted to compensate for the presence of the conductive components of the HIFU transducers. A T1‐weighted (T1‐W) sliding window dual‐echo GRE sequence monitored phase changes in the focal zone of each transducer. High‐intensity (2400 W/cm^–2), short duration (<1.5 seconds) exposures produced subtherapeutic temperature rises.

Results

For T1‐W images, signal‐to‐noise ratio (SNR) improved by 40% as a result of quartering the conductive surface of the piezoceramic transducer. A piezocomposite transducer showed a further 28% improvement. SNRs for an endocavitary coil in the focal plane of the HIFU trans‐ducer (4 cm from its face) were three times greater than from a phased body array coil. Local shimming improved uniformity of phase images. Phase changes were detected at subtherapeutic exposures.

Conclusion

We combined a HIFU transducer with an MR receiver coil in an endocavitary probe. SNRs were improved by quartering the conductive surface of the piezoceramic. Further improvement was achieved with a piezocomposite transducer. A phase change was seen on MR images during both subtherapeutic and therapeutic HIFU exposures. J. Magn. Reson. Imaging 2007. © 2007 Wiley‐Liss, Inc.     

Artificial neural networks (ANNs) for differential diagnosis of interstitial lung disease: results of a simulation test with actual clinical cases

RATIONALE AND OBJECTIVES: To evaluate the performance of an artificial neural network (ANN) scheme with use of consecutive clinical cases and its effect on radiologists with an observer test. MATERIALS AND METHODS: Artificial neural networks were designed to distinguish among 11 interstitial lung diseases on the basis of 26 inputs (16 radiologic findings, 10 clinical parameters). Chest radiographs of 96 consecutive cases with interstitial lung disease were used. Five radiologists independently rated their radiologic findings on the 96 chest radiographs. Based on their ratings of radiologic findings and clinical parameters obtained from the hospital information system, the output values indicating the likelihood of each of the 11 interstitial lung diseases were determined. Subsequently, 30 cases were selected from these 96 cases for an observer test. Five radiologists marked their confidence levels for diagnosis of 11 possible diseases in each case without and with ANN output. The performance of ANNs and radiologists was evaluated by receiver operating characteristic analysis based on their outputs and on confidence levels, respectively. RESULTS; The average Az value (area under the receiver operating characteristic curve) indicating ANN performance for the 96 consecutive cases was 0.85 +/- 0.03. The average Az values indicating radiologists' performance without and with ANN outputs were 0.81 +/- 0.11 and 0.87 +/- 0.06, respectively. The diagnostic accuracy was improved significantly when radiologists read chest radiographs with ANN outputs (P < .05). CONCLUSION: Artificial neural networks for differential diagnosis of interstitial lung disease may be useful in clinical situations, and radiologists may be able to utilize the ANN output to their advantage in the differential diagnosis of interstitial lung disease on chest radiographs.