雌激素对慢性间歇性低氧大鼠颏舌肌收缩功能及超微结构的影响

目的:探讨雌激素对慢性间歇性低氧大鼠颏舌肌等长收缩功能及肌细胞超微结构的影响. 方法:健康雄性SD大鼠40 只,随机分为正常对照组(NC)、慢性间歇性低氧组(CIH)、低、中、高剂量雌激素干预组(LE、ME、HE).后4 组建立CIH大鼠模型(8 h/d,5 周),同时LE、ME、HE组分别予苯甲酸雌二醇0.1、0.2、0.3 mg/kg(2 次/周)肌肉注射,NC、CIH组予无菌橄榄油 0.2 ml/次肌肉注射.第5 周末电刺激法测定各组大鼠颏舌肌等长收缩功能的变化,透射电镜观察肌细胞超微结构的改变.结果:各组大鼠颏舌肌单刺激收缩、强直收缩最大张力无明显变化;诱导疲劳试验中各时间点颏舌肌抗疲劳性:CIH组显著低于NC组(P＜0.01),ME、HE组明显高于CIH组(P＜0.05,P＜0.01);颏舌肌细胞超微结构:CIH组肌原纤维结构紊乱,部分肌丝溶解、消失,线粒体水肿,嵴断裂,空泡变;ME、HE组肌原纤维病变减轻,部分线粒体水肿,嵴减少.结论:慢性间歇性低氧可破坏大鼠颏舌肌细胞超微结构,降低抗疲劳能力;中、高剂量雌激素可不同程度逆转低氧对肌细胞超微结构及抗疲劳能力的损伤,保护低氧大鼠颏舌肌的抗疲劳能力.     

雌激素对慢性间歇性低氧大鼠颏舌肌线粒体细胞色素C氧化酶活性及其亚基表达的影响

目的 通过研究雌激素对慢性间歇性低氧(chronic intermittent hypoxia,CIH)大鼠颏舌肌线粒体细胞色素C氧化酶(cytochreme C oxidase,COX)活性及其亚基(COX Ⅰ、COXⅣ)表达的影响,探讨阻塞性睡眠呼吸暂停低通气综合征(OSAHS)发病中雌激素所起的作用.方法 3个月龄健康雄性SD大鼠48只,分为正常对照组(NC组)、慢性间歇性低氧组(CIH组)及慢性间歇性低氧+雌激素干预组(E+CIH组).后两组建立CIH模型,同时E+CIH组给予苯甲酸雌二醇0.2 mg/kg,NC组、CIH组给予无菌橄榄油0.2 ml/次,2次/周,肌肉注射.密度梯度离心法分离大鼠颏舌肌线粒体,极谱法测量COX活性;Western blotting分析COX Ⅰ和COXⅣ蛋白表达;实时聚合酶链反应(real-time polymerase chain action)法检测COX Ⅰ和COXⅣ的基因表达.结果 CIH组大鼠颏舌肌线粒体COX活性为(0.143±0.029)μkat/mg,与NC组[(0.273±0.058)μkat/mg]相比显著降低(P＜0.01),E+CIH组COX活性[(0.203±0.073)μkat/mg]较CIH组有所回升(P＜0.05),但仍显著低于NC组(P＜0.05).CIH组与E+CIH组COX Ⅰ蛋白表达分别为(10.789±8.144)和(25.593±11.108),与NC组(47.325±7.502)相比均显著降低(P＜0.01),且E+CIH组COX Ⅰ蛋白表达显著高于CIH组(P＜0.05).3组COXⅣ蛋白表达的差异无统计学意义(P＞0.05);3组COX Ⅰ、COXⅣmRNA表达量的差异无统计学意义(P＞0.05).结论 CIH可抑制大鼠颏舌肌线粒体COX蛋白表达及活性,而雌激素干预可部分恢复COX蛋白表达及活性.     

金雀异黄素对慢性间歇性低氧大鼠颏舌肌内氧化应激水平的影响

目的：探讨金雀异黄素对慢性间歇性低氧（CIH）大鼠颏舌肌氧化应激水平及抗氧化酶活性的影响。方法：8周龄健康雌性SD大鼠50只,去势1周后随机分为正常对照组（NC组）、慢性间歇性低氧组、慢性间歇性低氧＋低、中、高剂量金雀异黄素治疗组,每组10只。后4组建立CIH模型（8h/d,5周）。DCFH-DA荧光探针、硫代巴比妥酸法及底物催化法检测不同组颏舌肌内ROS和MDA的水平及抗氧化酶（超氧化物歧化酶酶,SOD）、过氧化氢酶（CAT）及谷胱甘肽过氧化物酶（GPx））的活性。结果：CIH导致大鼠颏舌肌内ROS和MDA的水平升高（P〈0.05）,金雀异黄素降低其水平（P〈0.05）。CIH导致大鼠颏舌肌内SOD、GPx及CAT的活性降低（P〈0.05）,低、中、高剂量的金雀异黄素恢复GPx和CAT的活性至正常水平（P〈0.05）,高剂量的金雀异黄素恢复SOD的活性至正常水平（P〈0.05）。结论：CIH导致大鼠颏舌肌内氧化应激水平的升高,金雀异黄素通过增强抗氧化酶的活性降低其氧化应激水平。     

雌激素对低氧大鼠颏舌肌肌型磷酸果糖激酶表达的影响

目的 探讨雌激素药物治疗对慢性间歇性低氧(chronic intermittent hypoxia,CIH)大鼠颏舌肌肌型磷酸果糖激酶(phosphofructokinase muscle-specific isoform,PFK-M)表达的影响,以期从能量代谢的角度探讨雌激素用于治疗阻塞性睡眠呼吸暂停低通气综合征(OSAHS)的可能机制.方法 选择2个月龄健康雄性SD大鼠50只,用随机排列表分为5组:正常对照组(NC)、慢性间歇性低氧组(CIH)、低剂量雌激素治疗组(LE)、中剂量雌激素治疗组(ME)、高剂量雌激素治疗组(HE),每组10只.后4组建立CIH模型(8 h/d,5周);后3组分别给予0.1、0.2、0.3 mg/kg的苯甲酸雌二醇肌肉注射,NC、CIH组给予无菌橄榄油肌肉注射.实时反转录聚合酶链反应(real-time RT-PCR)检测大鼠颏舌肌PFK-M mRNA表达;蛋白质印迹法分析PFK-M蛋白表达.结果 CIH组PFK-M mRNA和蛋白的表达量分别为(2.144±0.260)和(0.875±0.025),与NC组相比(1.000±0.259,0.413±0.013)均显著升高(P＜0.05).LE、ME、HE组PFK-M mRNA的表达量分别为(1.424±0.193)、(1.395±0.251)和(1.310±0.094),PFK-M蛋白的表达量分别为(0.638±0.015)、(0.576±0.017)和(0.505±0.021),与CIH组相比,3个雌激素治疗组的PFK-M mRNA和蛋白表达均显著降低(P＜0.05).3个雌激素治疗组间两两比较显示,HE组PFK-M蛋白表达量比LE组明显降低(P＜0.05),而PFK-M mRNA表达量的差异无统计学意义(P＞0.05).结论 CIH可导致大鼠颏舌肌内PFK-M转录和翻译水平的升高,而雌激素药物治疗可显著抑制PFK-M的过度表达,并呈现一定的剂量依赖趋势.     

雌激素对雌性大鼠舌下神经核及颏舌肌的影响

目的:研究SD大鼠外周血不同雌激素水平对舌下神经核自发放电及颏舌肌功能的影响,探讨雌激素影响上气道稳定性的中枢途径.方法:将30只SD大鼠随机分为假手术组(sham operation group,Sham)、去卵巢组(ovariectomized group,OVX)及去卵巢+雌二醇回补组(ovariectomized+ estrogen covering group,OVX+E2),采用玻璃微电极记录神经元放电,记录舌下神经核自发放电频率和最大幅值.采用BL-420生物学系统,检测颏舌肌功能:颏舌肌肌电的平均频率、最大频率、积分幅度、最大幅度;电刺激诱发肌电幅度、临界融合频率、50％强直收缩时程及阈刺激强度.采用SPSS13.0软件包对数据进行单因素方差分析.结果:OVX组舌下神经核的自发放电频率和最大幅值低于Sham组和OVX+E2组(P＜0.05);OVX组颏舌肌肌电最大频率、平均频率、积分幅度和最大放电幅度、50％强直收缩时程低于Sham组和OVX+E2组(P＜0.05),OVX组阈刺激强度和临界融合频率均显著高于Sham组和OVX+E2组(P＜0.05),OVX+E2组和Sham组各项指标相比无显著差异(P＞0.05).OVX组、Sham组、OVX+E2组颏舌肌的电刺激诱发肌电幅度无显著差异(P＞0.05).结论:外周血雌激素水平影响舌下神经核的兴奋性,进而影响颏舌肌功能.雌激素可能影响颏舌肌的收缩功能及抗疲劳能力.     

ERα在雌激素调控颏舌肌功能中的作用研究

目的:探索ERα在雌激素调控SD大鼠颏舌肌功能中的作用。方法:将30只8周龄雌性SD大鼠(250±25) g,随机分为假手术组(SHAM组),去卵巢+雌二醇组(OVX+E₂组),去卵巢+雌二醇+ERα拮抗剂MPP组(OVX+E₂+MPP组)(n=10)。通过卵巢切除与雌激素回补，在外周血建立稳定的雌激素水平大鼠模型；使用玻璃微电极检测SD大鼠舌下神经核团放电频率，BL-420E+生物机能实验系统检测颏舌肌肌电频率及幅值。结果:(1)舌下神经核放电频率：OVX+E₂+MPP组明显弱于OVX+E₂组(P<0.05)。(2)颏舌肌电频率及幅值：OVX+E₂+MPP组明显弱于OVX+E₂组(P<0.05)。结论：雌激素可能通过与ERα受体结合，影响舌下神经核放电，进而改变颏舌肌肌电功能。     

雌激素对雌性大鼠颏舌肌肌质网Ca^2＋-ATPase活性及其基因表达的影响

目的:观察雌激素对雌性大鼠颏舌肌细胞肌质网(sarcoplasmic reticulum, SR )Ca2 -ATPase活性及其基因表达的影响,探索其影响颏舌肌功能的分子生物学机制.方法:将30 只成年雌性SD大鼠随机分为对照组(control)、去卵巢组(ovariectomy,OVX)、去卵巢回补激素组(ovariectomy with 17β-estradiol, OVX E2).术后6 周,处死动物,提取颏舌肌SR.采用定磷法检测颏舌肌SR Ca2 -ATPase活性,荧光定量RT-PCR测定SR Ca2 -ATP酶 mRNA表达的变化.结果:OVX组血清雌激素水平及子宫湿重较对照组明显降低(雌二醇P<0.01;子宫湿重P<0.01),而OVX E2组与对照组相比差别无统计学意义(P>0.05).OVX组颏舌肌SR Ca2 -ATP 酶活性较对照组明显降低(P<0.01);OVX E2组较OVX组有明显提高(P<0.01)、与对照组相比差别无统计学意义(P>0.05).OVX组颏舌肌 SR Ca2 -ATPase mRNA表达较对照组明显降低(P<0.01);OVX E2组较OVX组有明显提高(P<0.01)、与正常组相比差别无统计学意义(P>0.05).结论:成年雌性大鼠雌激素水平的变化可引起颏舌肌SR Ca2 -ATP 酶活性及其mRNA表达的变化,雌激素可能通过该途径改变颏舌肌功能.     

外源性雌激素局部应用对去势雌性大鼠颏舌肌肌球蛋白重链基因表达的影响

目的:检测外源性雌激素局部应用对去卵巢大鼠颏舌肌肌球蛋白重链(myosin heavy chain,MHC)基因表达的影响,探讨雌激素水平与颏舌肌肌功能的相关性。方法:将30只7周龄健康雌性体质量210g左右的SD大鼠随机分为3组,每组10只。①去卵巢对照组(ovariectomized group,OVX组);②雌激素给药组(E2组);③假手术组(sham-operated group,SO组)。给药30d后取颏舌肌肌肉组织用荧光定量PCR(FQ-PCR)方法检测肌肉组织MHCⅡa、MHCⅡx、MHCⅡb、β-actin(内参照)基因表达。结果:去卵巢对照组颏舌肌MHCⅡb的表达明显高于假手术组(P<0.05),MHCⅡa、MHCⅡx的表达明显低于假手术组(P<0.05)。雌激素给药组颏舌肌MHCⅡb的表达明显低于去卵巢对照组(P<0.01),MHCⅡa、MHCⅡx的表达明显高于去卵巢对照组(P<0.05)。结论:雌激素水平变化能导致颏舌肌肌纤维类型的转化,从而改变肌肉功能。     

去卵巢雌性大鼠颏舌肌肌功能特征的研究

目的 检测雌性大鼠去卵巢后颏舌肌肌功能的变化，探索雌激素影响上气道稳定性的机制。方法 30只雌性SD大鼠随机分成三组：正常组、假手术组、去卵巢组。手术6周后，麻醉动物，切断双侧迷走神经，采用插入式双极针电极引导大鼠颏舌肌肌电；电脉冲刺激舌下神经中央支诱发颏舌肌收缩。原始信号经SMUP-PC型生物信号处理系统输入计算机，使用MF lab功能学实验软件包Version3．00分析，检测各实验动物颏舌肌的肌电强度、最大等长收缩力和70％强直收缩力的时程。结果假手术组颏舌肌的肌电强度、最大收缩力、70％强直收缩时程与正常组相比无显著性差异（P〉0．05），去卵巢组颏舌肌的肌电强度、最大收缩力、70％强直收缩时程较正常组明显降低（P〈0．05）。结论 雌性大鼠去卵巢后颏舌肌肌功能降低，雌激素可能通过增强颏舌肌肌功能的途径对上气道稳定性发挥保护作用。     

去卵巢雌性大鼠颏舌肌雌激素受体表达的研究

目的:检测分析去卵巢雌性大鼠颏舌肌内雌激素受体的表达及其因雌激素水平而变化的规律。方法:30只雌性SD大鼠随机分成3组:正常组、假手术组、去卵巢组。采用SP法检测各组大鼠颏舌肌细胞内雌激素受体ERα、ERβ的表达,并分析比较各组间差异。结果:正常雌性大鼠的颏舌肌细胞存在ERα和ERβ的阳性表达,ERβ的表达明显较ERα弱(P<0.05);去卵巢组大鼠颏舌肌细胞ERα的表达较正常组显著降低(P<0.001),但ERβ的表达无显著变化(P>0.05)。结论:雌性大鼠颏舌肌细胞内存在雌激素受体的表达,其中ERα的表达明显受雌激素水平的影响。     

Effects of phytoestrogens on genioglossus contractile properties in ovariectomized rats exposed to chronic intermittent hypoxia may be independent of their estrogenicity

Chronic intermittent hypoxia (CIH) is a frequent feature of obstructive sleep apnea/hypopnea syndrome (OSAHS), and it may alter upper airway muscle endurance. We have previously reported the positive effects of estrogen on genioglossus fatigue resistance in rats. Our present study was designed to evaluate the effects of two phytoestrogens - genistein and coumestrol - on genioglossus contractile function and estrogen receptor (ER) expression in female rats exposed to CIH. Eight-wk-old female rats were ovariectomized and exposed to CIH for 5 wk. Genistein and coumestrol, respectively, were administered by intraperitoneal injection, at a dose of 2.5 mg kg(-1) d(-1), during the last 4 d of exposure to CIH. The contractile properties of the genioglossus were measured. Real-time RT-PCR and western blotting were performed to determine the expression of ERs in the genioglossus. Phytoestrogens were found to significantly increase genioglossus fatigue resistance, the effect of genistein being more powerful than that of coumestrol. However, higher levels of ER mRNA and protein were detected in the coumestrol group than in the genistein group. We conclude that phytoestrogens, especially genistein, could improve the endurance of the genioglossus muscle in ovariectomized rats exposed to CIH, and this effect is, in part, not related to its estrogenic action.     

Genistein attenuates genioglossus muscle fatigue under chronic intermittent hypoxia by down-regulation of oxidative stress level and up-regulation of antioxidant enzyme activity through ERK1/2 signaling pathway

Oral Diseases (2011) 17 , 677–684 Objective: This study aims to investigate the effects of genistein on contractile properties of genioglossus under chronic intermittent hypoxia (CIH) conditions and its relationship with oxidative stress, antioxidant enzyme, and ERK1/2 signaling pathway. Materials and Methods: Fifty female Sprague–Dawley rats were randomly divided into five groups 1 week after ovariectomy: the normal control group, the CIH group, the CIH with low‐dose, medium‐dose, and high‐dose genistein groups. Rats in the latter four groups were exposed to CIH for 5 weeks. Twitch tension, tetanic tension, and fatigue resistance of genioglossus were investigated. Malondialdehyde (MDA) and mitochondrial reactive oxygen species (ROS), enzymatic activity of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), and ERK1/2 were detected. Results: Muscle fatigue resistance and enzymatic activity of GPx, CAT, and SOD were reduced after CIH exposure and improved by different doses of genistein at different degrees. CIH increased the level of ROS and MDA, and they were returned to normal by genistein. The expression of phospho‐ERK1/2 is opposite to the changes in muscle fatigue resistance. Conclusion: Chronic intermittent hypoxia decreases fatigue resistance of genioglossus, and genistein treatment reverses the fatigability of genioglossus by down‐regulation of oxidative stress level and up‐regulation of antioxidant enzymatic activity probably through ERK1/2 signaling pathway.     

17β-雌二醇对骨质疏松状态下牙种植体骨整合的影响

目的：研究17β-雌二醇（17β-E2）对骨质疏松大鼠种植体-骨界面骨整合指数的影响。方法：6月龄未交配SD雌性大鼠随机分为3组,A：假去势手术组（SHAM）,B：去势手术非药组（OVX）,C：17β-E2治疗组（E2）,每组13只。将后两组行卵巢切除术,并在卵巢切除术后1．5个月对所有大鼠右股骨远中种植纯钛螺钉,E2组在种植术后-周开始给予17β-E2（30μg／kg）治疗。分别于种植术后1．5个月及3个月,随机处死各组动物的-半。处死后,取大鼠右股骨,沿种植体长轴将种植体连同骨组织平均切为两半,扫描电镜观察与测量种植体-骨结合骨接触率即骨整合指数（OI）。结果：1．5个月与3个月处死时,E2组与SHAM组骨整合指数大致相同（E2组略高）,它们明显高于OVX组如〈0．05）。各组大鼠不同时期处死时,骨整合指数大致相同0〉0．05）。结论：1．骨质疏松改变可使大鼠种植体-骨界面骨整合指数降低。2．17β-E2可使骨质疏松大鼠种植体-骨界面骨整合指数增加。3．骨整合完成后,延长时间并不能使骨整合指数增加。     

Effects of oestrogen on sarcoplasmic reticulum Ca-ATPase activity and gene expression in genioglossus in chronic intermittent hypoxia rat

This study was designed to investigate the effects of oestrogen on sarcoplasmic reticulum (SR) Ca²⁺-ATPase activity and gene expression in ovariectomised rats under the condition of chronic intermittent hypoxia (CIH). Thirty-two female Sprague-Dawley rats were randomly divided into four groups: the normal control group (NC), the CIH group (CIH), the CIH-ovariectomised group (CIH + OVX), and the group of CIH-ovariectomised rats receiving estradiol replacement (CIH + OVX + E₂). Rats in the latter three groups were exposed to CIH for 5 weeks. The animals were killed before genioglossus (GG) was rapidly excised, and their body and uterus mass were determined. Estradiol level was detected by radioimmunoassay. SR Ca²⁺-ATPase (SERCA) activity was observed by detecting inorganic phosphorus ion, and the SERCA mRNA level was measured using real-time quantitative polymerase chain reaction (real-time PCR). It was found that, compared with the NC group, the SERCA activity and mRNA level were remarkably reduced (p < .01) in the CIH group. And compared with the CIH group, the SERCA activity and mRNA level were also significantly reduced (p < .01) in the CIH + OVX group. Meanwhile, the SERCA activity and mRNA level significantly increased (p < .01) in the CIH + OVX + E₂ group compared with the CIH + OVX group, but lower than those in the NC group (p < .01). The results showed that CIH could reduce the SERCA activity and mRNA expression, and oestrogen-deficiency could exacerbate this effect; whilst estradiol replacement can partially reverse the effect of CIH in ovariectomised rats.     

牙本质肩领形态及纤维桩的长度对患牙抗折强度影响的研究

目的：评价不同牙本质肩领形态及纤维桩的长度对患牙抗折强度的影响.方法：选择80颗新近拔除的单根下颌前磨牙,经根管治疗后随机分为8组,每组10颗.A1组：牙本质肩领-无纤维桩,A2组：牙本质肩领-5mm纤维桩,A3组：牙本质肩领-7mm纤维桩,A4组：牙本质肩领-9mm纤维桩; B1组：无牙本质肩领-无纤维桩,B2组：无牙本质肩领-5mm纤维桩,B3组：无牙本质肩领-7mm纤维桩,B4组：无牙本质肩领-9mm纤维桩;所有样本经复合树脂堆核后进行烤瓷冠修复.使用万能材料测试机对样本进行抗折强度测试,并采用SPSS 13.0对测试结果进行统计分析.结果：牙本质肩领组的患牙抗折强度显著高于无牙本质肩领组（P.＜0.05）,牙本质肩领组患牙抗折强度由大到小排序为A4＞A3＞A1＞A2,差异无统计学意义（P＞0.05）.无牙本质肩领组患牙抗折强度由大到小排序为B4＞B3＞B2＞b1,B1-B2组,B3-B4组差异无统计学意义（P＞0.05）,其余各组差异均有统计学意义（P＜0.05）.结论：尽量保留剩余牙体组织,来提高根管治疗后患牙的抗折强度.当无牙本质肩领存在时,可通过增加纤维桩的长度来增加修复体的固位力,并提高其抗折强度.     

Effects of phytoestrogen genistein on genioglossus function and oestrogen receptors expression in ovariectomized rats

Objective

This study was designed to investigate the effects of genistein on genioglossal muscle function and the expression of oestrogen receptors (ERs) in the ovariectomized rats.

Design

Fifty female Sprague-Dawley rats were randomly divided into 5 groups: the control group (SHAM), the ovariectomized group (OVX), the ovariectomized rats receiving low genistein dosage (OVX+L), the moderate genistein dosage group (OVX+M) and the high genistein dosage group (OVX+H). Oestradiol level was detected by radioimmunity. The isometric twitch tension (P_t) and tetanic tension (P₀) of the GG muscle were measured in response to electrical field stimulation. The expression of ERs on the mRNA and protein levels was measured by real-time PCR and western blot respectively.

Results

Ovariectomy decreased muscle fatigue resistance and the expression of different ERs significantly. Genistein treatment resulted in a dose-dependent protective effect on muscle fatigability and a parallel dose-responsive increase in the expression of oestrogen receptors mRNA and protein levels in genioglossus, with larger effects on oestrogen receptor beta vs. alpha. In contrast to the improvements in fatigability, there was no treatment effect on isometric twitch or tetanic tensions.

Conclusion

The results indicated that genistein increased muscle fatigue resistance in addition to effects on receptors, and the up-regulation of receptors expression may be a possible mechanism by which genistein improved fatigue.     

雌激素对骨质疏松状态下牙种植体周围骨组织内BMP2含量的影响

目的：研究系统应用17β-雌二醇对骨质疏松大鼠种植体周围骨组织BMP2含量的影响。方法：42只6月龄未交配SD雌性大鼠平均分为3组,每组14只,即A：假去势手术组（SHAM）,B：去势手术非用药组（OVX）,C：去势手术雌激素治疗组（E2）。将后两组大鼠卵巢切除后1.5月对其右股骨远中行牙种植手术,E2组在种植术后一周开始给予17β-雌二醇（30ug/kg）治疗。分别于种植术后1.5月及3月,随机处死各组动物的一半。处死后,取种植部位做脱钙切片,进行免疫组化染色,对种植体周围骨组织的BMP-2含量进行定量分析。结果：种植术后1.5月及3月处死时,各组BMP2含量由高到低的顺序为：E2组、SHAM组、OVX组,统计差异显著。同时,各组BMP2含量均较1.5月处死时显著增高,统计差异显著。结论：1骨质疏松改变可使大鼠种植体-骨界面BMP2含量降低。2 17β-estradiol可使骨质疏松大鼠种植体-骨界面BMP2含量增加。3雌激素长期用药的效果优于短期用药。     

去势大鼠股骨与腰椎骨密度改变的比较研究

目的：研究大鼠去势后股骨与腰椎骨密度的变化规律与相关性.方法：6月龄未交配SD雌性大鼠41只,随机分为2组即：A假去势手术组（SHAM）,n=20只.B去势手术组（OVX）,n=21只.对B组进行卵巢切除术.采用双能X线骨密度测量仪分别在卵巢切除术前、术后1.5个月、3个月及4.5个月测量各组大鼠腰椎3-5及左右股骨远中1/2骨密度,并对骨密度值进行相关性研究.结果：A组大鼠股骨与腰椎骨密度值在各时间测量点无显著差异（P＞0.05）,B组大鼠股骨与腰椎骨密度随术后时间延长而降低,差异显著（P＜0.01）.A、B两组大鼠股骨与腰椎骨密度各时间点均显著相关（P＜0.01）.结论：卵巢切除术可致大鼠双股骨远中及腰椎骨密度降低,是制备绝经后骨质疏松动物模型的可靠方法,大鼠腰椎与双股骨骨密度改变具有相关性.