晚期喉癌边缘DNA含量和增殖细胞核抗原表达

目的 探讨晚期喉癌边缘ＤＮＡ含量和增殖细胞核抗原（ＰＣＮＡ）表达与喉癌手术预后的关系。方法 通过ＰＣＮＡ表达和ＤＮＡ含量检测，对３４例晚期喉癌边缘进行观察。结果 肿瘤边缘１．０、０．５、０ｃｍ处粘膜，ＰＣＮＡ指数分别为８．６２％，１７．７６％和５０．３２％。ＤＮＡ指数分别为０．９８、１．０８２和１．４３６。统计学分析１．０、０．５ｃｍ处粘膜０ｃｍ处粘膜差异有显著性（Ｐ〈０．０１）。结论 中、重度非     

c-myc癌基因蛋白在喉癌及癌旁不同距离组织中的表达研究

应用枸橼酸－ＬＳＡＢ－微波免疫组化法，检测ｃ－ｍｙｃ癌基因蛋白在喉癌、边缘区及癌旁０．５、１．０、１．５、２．０ｃｍ处粘膜和４例正常喉粘膜中的表达。结果：ｃ－ｍｙｃ蛋白在喉癌组织中的阳性表达率为９６．７％（２９／３０），与正常组织相比有极显著性差异（Ｐ〈０．０１）；在癌旁２．０ｃｍ处与１．５ｃｍ以内组织的表达存在着显著性差异（Ｐ〈０．０５）。ｃ－ｍｙｃ表达依正常粘膜→癌旁组织→癌呈逐步递增趋势。ｃ     

喉癌手术安全切缘的免疫组化研究

目的：从基因蛋白水平角度研究喉癌手术安全切缘。方法：应用 Ｌ Ｓ Ａ Ｂ免疫组化法,检测了ｒａｓ、ｃｍ ｙｃ、ｐ５３ 癌基因在３０ 例喉癌,边缘区,癌旁０．５、１．０、１．５、２．０ ｃｍ 粘膜处和 ４ 例正常喉粘膜中的表达情况。结果：三种癌基因产物的单独及联合表达阳性率依正常粘膜→癌旁２．０ ｃｍ →１．５ ｃｍ →１．０ ｃｍ →０．５ ｃｍ →边缘区→癌顺序递增,且癌基因蛋白的表达在距肿瘤０．５ ｃｍ 以内的癌旁组织与癌旁组织１．０ ｃｍ 处有显著性差异（ Ｐ ＜ ０．０５）。结论：距肿瘤０．５ ｃｍ 内的癌旁组织应视为癌前期病变,以切缘距肿瘤０．５ ｃｍ 作为喉癌手术安全切缘的标准较为合适     

增殖细胞核抗原在喉上皮癌前病变中的表达及意义

用免疫组化技术对１１例喉上皮单纯性增生（ＳＨＥ），３２例非典型性增生（ＡＨＥ）及４２例喉鳞状细胞癌（ＬＳＣＣ）的增殖细胞核抗原（ＰＣＮＡ）表达进行了原位检测。结果表明；在ＳＨＥ、ＡＨＥ及ＬＳＣＣ中，ＰＣＮＡ指数分别为９．５７％、２７．３３％、６８．０５％，差异极有显著性意义（Ｐ＜０．００１）。在轻、中、重度ＡＨＥ中，ＰＣＮＡ指数分别为１３．７９％、３０．８４％、３９．９４％，差异有非常显著性意义（Ｐ＜０．０１）。提示ＰＣＮＡ表达程度与非典型性增生程度密切相关，是反映细胞增殖状态的生物标志，ＰＣＮＡ组化研究有助于认识喉癌前病变的本质，发展趋势及喉癌的早期诊断。     

增殖细胞核抗原在喉上皮癌前病变中的表达及意义

用免疫组化技术对１１例喉上皮单纯性增生（ＳＨＥ），３２例非典型性增生（ＡＨＥ）及４２例喉鳞状细胞癌（ＬＳＣＣ）的增殖细胞核抗原（ＰＣＮＡ）表达进行了原位检测。结果表明：在ＳＨＥ、ＡＨＥ及ＬＳＣＣ中，ＰＣＮＡ指数为９．５７％、２７．３３％、６８．０５％，差异极有显著性意义（Ｐ＜０．００１）。在轻、中、重度ＡＨＥ中，ＰＣＮＡ指数分别为１３．７９％、３０．８４％，３９．９４％，差异有非常显著意义（Ｐ＜０     

喉上皮增生性病变中PCNA及c-erbB-2的表达及其临床意义

目的：研究ＰＣＮＡ及ｃ－ｅｒｂＢ－２在喉上皮增生性病变中的表达与增生程度的关系。方法：对１０例上皮单纯性增生、２６例异型增生及３０例喉鳞状细胞癌石蜡包埋标本,用免疫组化ＳＰ法检测其ＰＣＮＡ及ｃ－ｅｒｂＢ－２。结果：单纯性增生、异型增生和喉鳞癌间三者ＰＣＮＡ指数分别为６．６４％、２９．６３％及６２．６７％,差异有显著性意义（Ｐ〈０．０１）;单纯性增生病变与轻度异型增生间亦有显著性差异（Ｐ〈０．０１）     

喉癌EGFR和PCNA表达及DNA含量的研究

研究喉鳞癌中表皮生长因子受体、增殖细胞核抗原的表达和ＤＮＡ含量,分析各指标间的相关性及与喉癌临床生物学行为和预后的关系。方法：用免疫组化ＳＰ法和图像分析仪检测正常喉粘膜、喉鳞癌的ＥＧＦＲ、ＰＣＮＡ和ＤＮＡ指数,结合临床资料进行分析。结果：喉鳞癌的ＥＧＦＲ的阳性表达率为５４．８％,与正常喉组织的差异有统计学意义,但ＥＧＦＲ的阳性表达与喉癌的病理分级和５年生丰率无显著相关,而ＰＣＮＡ阳性表达和ＤＩ则随     

喉癌癌变过程中CyclineE的表达及临床病理学意义

目的：研究喉癌癌变过程中ＣｙｃｉｉｎＥ的临床病理学意义。方法：用免疫组化检测２０例正常喉粘膜，４０例喉不典型增生和６０例喉癌组织中ＣｙｃｌｉｎＥ的表达。结果：ＣｙｃｌｉｎＥ阳性表达定位于肿瘤细胞核。在喉癌癌变过程中，喉正常粘膜、不黄型增生病变和喉癌中ＣｙｃｌｉｎＥ阳性表达率分别为：５．０％（１／２０），２０．０％（８／４０）和４５．０％（２７／６０）（Ｐ〈０．００１）。喉癌淋巴结转移组Ｃｙｃｌｉｎ     

用吖啶橙荧光染色对喉癌细胞DNA和RNA的观察

采用吖啶橙荧光染色方法对７例喉正常粘膜上皮（ＮＥ）、２５例喉粘膜上皮非典型增生（ＡＨＥ）和４４例喉鳞状细胞癌细胞形态和ＤＮＡ、ＲＮＡ进行观察。结果证明吖啶橙荧光染色是一种可显示细胞分化程度的染色方法，对细胞ＤＮＡ、ＲＮＡ具有很强的特异性，可以显示细胞的形态、结构和两种核酸的含量变化。方法简便、迅速、图像清晰，显示了喉粘膜上皮不典型增生和喉癌ＤＮＡ、ＲＮＡ的变化特点，对喉癌早期诊断具有应用价值。     

喉近全切除术治疗晚期喉癌的临床疗效观察

目的 探讨晚期喉癌患者喉切除术后保留喉发声功能以提高生存质量,方法 对１７例晚期喉癌行喉近全切除术（Ｐｅａｒｓｏｎ手术）手术中,保留一侧杓状软骨及一条宽约１．５ｃｍ的与气管相连的粘膜条形成发声管,Ｎ０期患者规探查颈动脉分叉处淋巴结,根据冰冻病检结果而决定是否行颈淋巴清扫术,结果：术后１２例发声良地,除１例外均无明误吸现象,２年,３年及５年生存率分别为７０．６％,６２．４％,５０％,结论：Ｐｅａｒｓ     

喉癌前病变恶变机理的探讨

选自北京同仁医院耳鼻咽喉科1992年5月～1994年6月收集的标本61例，其中喉癌前病变标本34例，利用免疫组化（P(53)、PCNA）及原位杂交技术，重点对不同病变阶段的10例慢性肥厚性喉炎、13例喉角化症和11例喉乳头状瘤（成人）进行检测，并与16例喉单纯息肉和11例喉原位癌标本进行了比较。分析了各种检测的阳性表达率在病变发展过程中的变化规律，对喉癌前病变的转归进行评估和预测。喉单纯息肉、喉癌前病变和原位癌三种病变中，P(53)蛋白的表达率分别是0％，35．29％和63.64％；PCNA指数分别是6．32％、26．16％和62．02％。在喉癌前病变和原位癌中，HPV(16.18)的阳性表达率分别是32．40％和63．63％。在癌前病变中重度非典型增生与轻、中度非典型增生P(53)和PCNA的阳性表达率有明显的差异（P＜0.05）。     

增殖细胞核抗原(PCNA)、DNA含量及细胞核形态参数测定在鼻咽癌复发中的应用价值

应用增殖细胞核抗原（PCNA）单克隆抗体PC10免疫组化法，DNA含量及细胞核形态图像分析法，对58例鼻咽癌进行检测。结果显示，三种方法的检测结果具有较好的一致性，其中肿瘤细胞的DNA指数和DNA倍体分布最具有价值。结果表明，检测肿瘤细胞增殖活性对于判断鼻咽癌恶性变及估计患者的预后具有重要意义。     

DNA index,cellular proliferative activity and nucleolar organizer regions in cancers of the larynx

The DNA index, expression of cell-cycle-related proteins — proliferating cell nuclear antigen (PCNA, cyclin) and Ki-67 — and the content of silver-binding nucleolar organizer regions (AgNORs) were evaluated in 30 unselected consecutive primary squamous cell carcinomas of the larynx. Results were compared and subsequently related to histological grading, lymph node status, pT category, and pathological stage. DNA content was non-diploid in 9 cases (30%). Mean AgNOR counts per tumor ranged from 2.52 to 8.76. PCNA and Ki-67 expressions were similar in 10 cases (33%). In the remaining cases, PCNA-positive cells usually outnumbered Ki-67-positive cells. No significant correlation was found among DNA index, PCNA and Ki-67 expressions, and AgNOR counts. Although there was a positive trend when Ki-67 was compared with histological grading, findings were not statistically significant. In contrast, a significant correlation was found between DNA index and lymph node status (P = 0.035), with a higher incidence of neck node metastases in non-diploid tumors. These data suggest that tumor ploidy can be correlated with lymph node spread in laryngeal squamous cell carcinoma and might be used as an additional prognostic factor when planning treatment.     

Acoustic schwannoma and pregnancy: A DNA flow cytometric,steroid hormone receptor,and proliferation marker study

For a long time, it has been speculated that pregnancy stimulates the growth of acoustic schwannomas. To test this hypothesis, immunohistochemical stains for estrogen receptor, progesterone receptor, and proliferating cell nuclear antigen (PCNA) were performed. Flow cytometric studies for DNA ploidy and S-phase fraction determinations were also performed. The study subjects included 6 female patients with unilateral acoustic tumors; at the time of tumor removal, 1 woman was pregnant and the other 5 women were 2 to 10 months postpartum. The age-sex-matched control group consisted of 6 men and 12 nonpregnant women, all with acoustic schwannomas similar in size to those of the study group. The study found no statistically significant association between the presence or quantity of estrogen or progesterone receptors and pregnancy, DNA ploidy, proliferation indices, or clinical data. Based on PCNA indices, large tumors tended to be less “biologically active” than small lesions (P<.01). The authors concluded that pregnancy does not significantly stimulate the cellular growth of acoustic schwannomas.     

Heterogeneity of Squamous Cell Carcinomas of the Head and Neck-Analysis of Tumor Biologic Factors and Proliferation Rates

Specimens obtained from five different tumor regions in 12 patients who underwent surgery for squamous cell carcinoma (SCC) of the oropharynx were examined. The evaluation of each biopsy included quantitative DNA measurements based on image analysis, immunohistochemical assessment of proliferations markers (i.e., Ki67-MIB1, proliferating cell nuclear antigen [PCNA]), and morphological tumorfront grading. From single cell measurements, several DNA indices were derived which are known to reflect tumor aneuploidy. The results revealed a marked variation of proliferation and cellular differentiation in different regions of tumors and a wide intraindividual variation between particular tumors for all markers examined. There was good correlation between DNA data and proliferative cell fractions (Ki67 score, PCNA score). With the use of diagrams, three-dimensional distribution of proliferation rates and markers reflecting tumor aggressiveness within each tumor was obtained. The results confirmed previous clinical and histological observations that SCCs of the oropharynx are heterogeneous tumors. One might expect that the regions with increased proliferation and aggressiveness may predict the location of possible tumor recurrence.     

Prognostic value of cell proliferation markers,tumour suppressor proteins and cell adhesion molecules in primary squamous cell carcinoma of the larynx and hypopharynx

In an attempt to identify molecular prognostic markers, a series of laryngeal and hypopharyngeal carcinomas was examined for PCNA, Ki67, p27(Kip1), p53, E-cadherin and CD44 by immunohistochemistry and for DNA content by flow cytometry. No correlation was found between E-cadherin, CD44, p53 or DNA ploidy and the clinicopathological data. The fraction of cancer cells immunolabelled for p27(Kip1) correlated with tumour differentiation, but not with lymph node metastasis. In contrast, the PCNA, Ki67 and S-phase fractions of cancer cells were significantly higher in tumours with lymph node metastasis than in those without lymph node metastasis and were correlated with pathological T-stages and with tumour dedifferentiation. In univariate analysis, advanced pathological T-stage, lymph node metastasis and high fractions of cancer cells immunolabelled for PCNA or Ki67 inversely correlated with overall and disease-free survival. In multivariate analysis, lymph node metastasis was the only factor significantly associated with poor survival. The data suggest that immunohistochemical investigation of PCNA and Ki67 and flow cytometric analysis of S-phase fractions may be useful predictive markers of biological aggressiveness in laryngeal carcinomas.