冠心病患者血浆cAMP、cGMP水平变化及临床意义

不稳定性心绞痛(UAP)和急性心肌梗死(AMI)是冠心病的严重类型,而冠状动脉栓形成是UAP、AMI的重要诱因.我们对不同类型冠心病患者血浆cAMP、cGMP浓度进行了检测,以探讨其在冠心病发病中的作用. 资料与方法:观察对象为1999年10月至2001年1月我院收治的冠心病患者,均符合国际心脏病学会及WHO1979年制定的诊断标准.其中稳定性心绞痛(SAP)组28例,男16例、女12例,年龄31～77岁、平均54± 12.7岁.UAP组48例,男27例、女21例,年龄30～82岁、平均56±15 .4岁.AMI组23例,男15例、女8例,年龄36～78岁、平均58±13.3岁 .对照组为26例健康人,无心绞痛病史,心电图、Holter超声心动图正常,男1 5例、女11例,年龄34～72岁、平均年龄52±12.4岁.     

肝病患者血浆cAMP、cGMP和ANP变化的意义探讨

为了探讨肝病中cAMP、cGMP和ANP的变化规律及其相互间的关系，本文用放射免疫法测定肝炎，代偿期和失代偿期肝硬化病人的血浆cAMP、cGMP和ANP含量并与正常对照，发现肝炎组血浆cAMP与正常组比较无显著差异。cAMP／cGMP比值依次为肝炎组＞代偿期肝硬化＞失代偿期肝硬化。失代偿期肝硬化cAMP和ANP显著高於正常和代偿期肝硬化。提示cGMP可能是ANP的第二信使。cAMP／cGMP比值可作为肝病严重程度的判断指标。     

急性脑梗塞患者血浆cAMP和cGMP的含量变化及其临床意义

用放射免疫分析法对８４例急性脑梗塞患者（脑梗塞组）及６０例同龄健康者（对照组）的血浆环磷酸腺苷（ｃＡＭＰ）和环磷酸鸟苷（ｃＧＭＰ）含量进行了测定。结果显示，脑梗塞组血浆ｃＡＭＰ和ｃＧＭＰ含量及其比值均低于对照组，ｃＡＭＰ降低明显（Ｐ〈０．０１），ｃＧＭＰ降低轻微（Ｐ〉０．０５），其ｃＡＭＰ含量降低及ｃＡＭＰ／ｃＧＭＰ比值减小与病情呈正相关，提示检测血浆ｃＡＭＰ和ｃＡＭＰ含量有助于判断脑梗塞病情，指     

实验性癫痫大白鼠脑组织cAMP,cGMP含量的变化及意义

目前，癫痫的发病机制还未完全阐明。环磷酸腺苷（ｃＡＭＰ）、环磷酸鸟苷（ｃＧＭＰ）在脑组织中含量丰富，其微量变化即可引发脑细胞放电；癫痫是神经元的异常放电，有学者认为ｃＡＭＰ、ｃＧＭＰ的变化可能在癫痫发病机制中起重要作用。为此，我们对实验性癫痫大白鼠脑...     

磁场对AMI大鼠心肌ATP及血浆cAMP、cGMP的影响

目的探讨磁场对AM I的保护作用。方法将大鼠随机分为5组即:空白对照组、磁场对照组、AM I组、AM I药物(心得安)治疗组和AM I磁场治疗组。采用虫荧光素酶法及放射免疫技术法对实验性大鼠进行心肌ATP含量、及血浆cAMP、cGMP的测定。结果AM I磁场治疗组心肌ATP明显高于AM I组(P<0.01)与药物治疗组相近似。AM I的血浆cAMP、cGMP含量明显高于空白对照组(P<0.01)及磁场对照组,AM I磁场治疗组与药物治疗组明显低于AM I组(P<0.01)。结论磁场能降低AM I大鼠cAMP、cGMP的含量,增加心肌ATP含量,对心肌具有保护作用,这也为磁场用于AM I的治疗提供了实验依据。     

肝细胞生长因子与肝细胞再生

肝脏是具有极强再生能力的器官。自Ｈｉｇｇｉｎｓ和Ａｎｄｅｒ－ｓｏｎ报道大鼠部分肝切除术后肝细胞迅速再生实验以来,学者们开展了众多关于肝细胞再生机制的研究。目前,就肝细胞再生启动与终止的机制尚未最终明确。肝细胞增生的过程是通过超乎想象的复杂的调控实现的。肝细胞增生大体可分为３个阶段①增殖启动过程（Ｇ０～Ｇ１期）,相关因子有ＩＬ－１,ＩＬ－６和ＴＮＦ－α;＠增殖促进调节过程（Ｇ１～Ｓ期）,相关因子有肝细胞生长因子（ＨＧＦ）,表皮生长因子（ＥＧＦ）／转化生长因子α（ＴＧＦα）等;③增殖停止过程（Ｇ１～…     

糖尿病大鼠坐骨神经cAMP、cGMP与微循环相关性及益气活血通络中药的干预作用

目的观察糖尿病(DM)大鼠坐骨神经环磷酸腺苷(c AMP)、环磷酸鸟苷(c GMP)与微循环相关性及益气活血通络方的干预作用。方法采用链脲佐菌素复制DM大鼠模型,并随机分为模型组、中药组、弥可保组,同时设正常对照组。观察益气活血通络中药对大鼠血液流变学、坐骨神经c AMP、c GMP含量的影响。结果 DM大鼠血液流变学、坐骨神经c AMP、c GMP含量均发生改变,益气活血通络方药对二者有明显改善作用。结论 DM大鼠血液流变学与坐骨神经改变c AMP、c GMP含量具有相关性,益气活血通络中药通过对二者的改善从而防治DM周围神经病变。     

肝细胞生长素的研制及临床应用

近年国内已有应用“人胎肝细胞悬液”(FLC)治疗重症肝炎及慢性活动性肝炎获得较好疗效的报道。但临床广泛应用FLC尚存在两个亟待解决的问题:(1)人胎肝细胞来源困难;(2)胎肝细胞活性难以较长时间保存。我们从Labrecque氏研究肝     

复方丹参滴丸预防治疗TIA的临床观察及对血浆cAMP、cGMP的影响

目的 :观察复方丹参滴丸预防治疗短暂性脑缺血发作 (TIA)的临床疗效及对血浆cAMP、cGMP的影响。方法 :选择 180例TIA病人 ,随机分为 3组 ,均口服给药 ,Ⅰ组 60例 ,每日1次 ,每次 10粒 ;Ⅱ组 60例 ,每日 2次 ,每次 10粒 ;Ⅲ组 60例 ,每日 3次 ,每次 10粒。每例病人均 3个月随访1次 ,共随访 18个月 ,同时采用放射免疫分析法 ,测定 3组血浆中的cAMP、cGMP含量。结果 :3组分别发生卒中的例数为 6例、5例、4例 ,副作用分别为 1例、2例、4例 ,未出现牙龈出血、鼻出血及皮下出血病例 ,无统计学意义 (P >0 .0 5 )。 3组cAMP分别为 2 1.2 2 pm /ml± 3 .94pm /ml、2 2 .5 0pm /ml± 3 .16pm /ml、2 3 .10pm /ml± 7.70pm/ml;cGMP分别为3 .67pm /ml± 1.18pm /ml、4.74pm /ml± 2 .12 pm /ml、4.60 pm/ml±0 .70 pm /ml,3组进行两两比较 ,并与本院正常值比较 ,均无统计学意义(P >0 .0 5 )。结论 :复方丹参滴丸在TIA的二级预防中 ,有肯定的疗效 ,且无明显副作用 ,可作为预防和治疗脑血管疾病的药物之一。     

促肝细胞生长素致过敏性休克1例

1病例资料 患者，男，51岁，农民。因“腰腿部疼痛”于2009年5月24日住院治疗。入院后体检及综合辅检诊断“腰椎病；原发性肝癌”。在综合基础保肝治疗的同时，于2009年5月26日用促肝细胞生长素（PHGF）100mg加入5％葡萄糖液250ml静脉滴注，     

Changes of plasma cAMP and cGMP levels in chronic atrophic gastritis patients with spleen-qi deficiency

AIM: To investigate the relationship between plasma cAMP and cGMP levels and spleen-qi deficiency in chronic atrophic gastritis. METHODS: Plasma cAMP and cGMP levels were detected in chronic atrophic gastritis patients with spleen-qi deficiency (n = 87). Thirty patients underwent self-controlled study prior to and after 1-2 courses of Weiweian therapy, with each course lasting 3 mo. The samples were detected using ¹²⁵I-labelled cAMP and cGMP kits. RESULTS: Plasma cAMP and cGMP levels in all cases of chronic atrophic gastritis patients with spleen-qi deficiency syndrome were lower than or at the lower margin level. In the 30 patients receiving Weiweian therapy, both plasma cAMP and cGMP levels increased significantly after the therapy as compared with those prior to the therapy (P < 0.01, and < 0.05). CONCLUSION: Measurements of plasma cAMP and cGMP levels are of great importance in CAG patients with spleen-pi deficiency, and Weiweian therapy can correct the spleen-qi deficiency. Furthermore, plasma cAMP may serve as a prognostic factor in CAG, and it might prevent CAG from progressing into gastric cancer since plasma cAMP is low in gastric cancer.     

Influence of metabolic control of insulin-dependent diabetes on plasma nucleotide levels (cAMP, cGMP) during bicycle exercise

Plasma concentrations of cyclic nucleotides (cAMP, cGMP) were measured before and during bicycle exercise in 8 well-controlled (mean pre-exercise blood glucose 5.3 mmol/l; HbA1 8.6%) and 8 moderately controlled (mean pre-exercise blood glucose 12.2 mmol/l; HbA1 10.8%) patients aged 18-32 years with insulin-dependent diabetes mellitus (IDDM) and in a group of non-diabetic control subjects matched for age and sex. Pre-exercise plasma cAMP concentrations and the rise with exercise were similar in all study groups. Significantly lower resting cGMP levels were found in well-controlled IDDM patients (3.5 +/- 0.3 pmol/ml, mean +/- SEM) compared to controls (5.6 +/- 1.1 pmol/ml; p less than 0.05) and moderately controlled IDDM patients (5.6 +/- 1.0 pmol/ml; p less than 0.05). By contrast, plasma cGMP levels increased during exercise in the diabetics but not in the controls. These findings indicate a significant difference in responses of plasma cGMP to exercise between IDDM patients and controls.     

刺激大鼠离断背根神经外周端对应皮区cAMP,cGMP释放的影响

目的 探讨循经感传的外周神经机制和跨节段感觉传入中的物质基础(cAMP和cGMP)。方法 利用刺激离断背根神经外周端大鼠模型,采用放射免疫分析法检测相应皮区皮瓣浸泡液中cAMP、cGMP含量。结果 刺激背根神经外周端后,刺激侧皮瓣浸泡液中的cAMP含量(39.50±4.525fg/ml)明显高于对照侧(24.104±3.952fg/ml);刺激侧皮瓣浸泡液中的cGMP含量(6.684±1.215fg/ml)亦明显高于对照侧(2.784±0.548fg/ml)。结论 刺激背根神经可通过逆向传导促使相应末梢皮区释放cAMP和cGMP增多。cAMP和cGMP可能是循经感传与跨节段感觉传入中的共同分子基础。     

Selective Effect of High Arterial Pressure in Hypertension Upon Inhibition of cGMP Versus cAMP Mediated Vascular Relaxation

We tested the hypothesis as to whether elevated arterial pressure in hypertension alters cGMP, or cAMP, mediated vasorelaxation. Relaxation to nitroglycerin and isoproterenol was determined in isolated aortic rings from one-kidney, one clip hypertensive (1K1C), coarctation hypertensive (CH) and normotensive control (C) rats. Thoracic aortas from 1K1C and CH rats, as well as abdominal aortas from 1K1C rats, but not abdominal aortas from CH rats were exposed chronically (4–6 weeks) to elevated arterial pressure. Sensitivity of rings with and without endothelium to nitroglycerin was suppressed significantly only in vessels exposed chronically to high arterial pressure. Impaired sensitivity to nitroglycerin in abdominal rings from lKlC rats could not be abolished by exposure to 100 uM L-arginine, the substrate for production of NO by endothelial nitric oxide synthase, or 100 uM L-cysteine, the source of thiol groups required for the production of nitric oxide from nitroglycerin. Maximum relaxation to isoproterenol was impaired significantly in thoracic and abdominal rings, with and without endothelium, from lKlC and CH rats. Relaxation to 8-bromo-cGMP and dibutyryl cAMP was similar in abdominal rings from all groups. We conclude that impaired vasorelaxation to nitroglycerin and isoproterenol in hypertension involves mechanisms prior to activation of vascular smooth muscle cGMP-dependent and cAMP dependent protein kinase, respectively. Impaired cGMP, but not cAMP, mediated relaxation of aortas appears to result from their exposure to high arterial pressure per se. This effect does not appear to involve the vascular endothelium or vascular sources of thiols, but rather may reflect an effect of high arterial pressure to impair the ability of the artery to respond t o nitric oxide derived from nitroglycerin.     

Hepatocyte growth factor (HGF) regulates blood-testis barrier (BTB) in adult rats

We have studied the effects of HGF on BTB dynamics in adult rats. We demonstrate that, at stages VII-VIII of the epithelium wave when germ cells traverse the BTB, HGF reduces the levels of occludin and influences its distribution pattern and assembling. Moreover, we report that, at stages VII-VIII, HGF significantly increases the amount of active TGF-β and the amount of uPA present in the tubules. For the first time we report that, in the same stages, HGF reduces the amount of actin present in the BTB region, in which occludin levels are highest, and modifies the morphology of the actin cytoskeleton network. At the level of maximal intensity of occludin fluorescence, we report that HGF also modifies the colocalization of occludin and actin. Lastly, we demonstrate that HGF is maximally expressed at stages VII-VIII, whereas its levels fall in the subsequent stages.     

Alteration in canine cardiac basal levels of cAMP and cGMP, and elevated tissue PCO2 levels, induced by coronary ligation.

Forty dogs were subjected to coronary ligation by placing snares around the two consecutive diagonal branches of the left anterior descending coronary arteries. Both acute and chronic studies were made of intramyocardial gas tensions using the mass spectrometer. Other studies such as levels of cyclic nucleotides and myosin ATPase activity were made at the chronic periods. Following coronary ligation in dogs there was a significantly elevated tissue Pco₂ level in the ischemic region and left ventricular border zone as late as 5 weeks post-ligation. There was a severe decrease in cAMP, whereas cGMP was either the same as normal values or in some cases significantly elevated, in the ischemic area during this post-ligation time. These data were reflected in the border area of the left ventricle, but to a lesser extent. There was a minor, but significant decrease in myosin enzymatic V_max values in the border area of the left ventricle during the early post-ligation period when tissue Pco₂ levels were significantly elevated.     

肝细胞生长因子和丹参对大鼠急性肝损伤保护作用的实验研究

本文报道肝细胞生长因子(HGF)、丹参和两药联用对CCl_4所致大鼠肝损伤有保护作用,表现为血清ALT、α-肿瘤坏死因子(TNF-α)、丙二醛(MDA)、肝组织匀浆MDA、肝组织钙含量和血清及肝匀浆超氧化物歧化酶(SOD)活力及光镜、电镜下肝组织病变均有明显改善,且联用优于单用。本文还对HGF、丹参合用所起协同作用的机理作一简短的讨论。