解脲支原体的生长条件研究

目的 探讨解脲支原体（Uu)在液体培养基中的最佳营养条件和在固体培养基上形成菌落的最佳气体条件,方法 采用不同的pH值、不同的酵母及小牛血清含量配制解脲支原体培养基,将法国Merie-um生产的培养基作对比研究。结果 解脲支原体生长最适pH值为6.0-6.5;10%酵母提取液、10%小牛血清能加速解脲支原体的生长,90%N2、5%-10%CO2能促进解脲支原体菌落生长。结论 本研究为改进解脲支原体的培养质量、开发高效的支原体培养基及药敏检测板提供实验依据。     

解脲支原体的分离及生物学特性

本文报告从5份产妇阴道分泌物中分离到3株解脲支原体的试验结果.在改良的Taylor-Robinson培养基中解脲支原体生长迅速,液体培养基中12～24小时就能分解尿素产硷,使含有酚红的培养基变色,但培养基不混浊、亦不呈表面生长.0.45μm的微孔滤膜过滤后仍然出现前述的生长现象.固体培养基在37℃烛缸培养48小时整个琼脂平板产硷变红,却无可见的生长现象,用显微镜放大100倍方可见直径15～30μm、圆形、凸起由细小颗粒聚集而成的微小菌落,随着培养时间延长,菌落可增至60～80μm,某些菌落表现为典型的油煎蛋样.电子显微镜观察解脲支原体无细胞壁,是仅由一层浆膜包统的原核型生物.本文还就解脲支原体的生长条件、营养要求、液体中生长特点、生化反应及培养注意事项等进行了讨论.     

一些培养基添加剂对解脲支原体生长的影响

对几种培养基添加剂对解脲支原体生长的影响进行研究。结果表明：狗血清、鸡蛋黄、鸡蛋清与小牛血清一样，有促进解脲支原体生长的作用，培养基中添加１０％狗血清、２～４％鸡蛋黄，２０％鸡蛋清，或１％～２％鸡蛋黄和５％～１０％鸡蛋清，可获得与添加１０％小牛血清相似的效果；鲜酵母浸液也能促进其生长，脲浓度从０．１％增至０．３％，有利于其生长，而蛋白胨酵母浸汁（工业产品）则有抑制作用。     

解脲脲原体引起血流感染1例

目的 分析安溪县医院1例流产后解脲脲原体引起的血流感染,及时为临床诊断及治疗提供依据。方法 回顾性分析该例解脲脲原体血流感染患者鉴定为解脲脲原体的整个诊断过程,收集了来自临床的基本资料、实验室诊断资料,包括血培养曲线特征、培养液瑞氏染色、支原体液体鉴定药敏及固体培养基菌落特征,以及靶向DNA测序结论。通过对上述资料的综合分析,优化血培养支原体的检测诊断流程及方法。结果 该例患者临床表现为术后发热38.5℃,CRP:14.85 mg/L,WBC:14.33×10⁹/L,NET:85.40%,PCT:0.12 ng/m L,IL-6:665.6 pg/m L。血培养3 d报阳,革兰染色未见菌体,瑞姬染色可见细沙样紫色菌体。接种于血平板、支原体固体、液体培养基,血平板培养48 h及5 d均未见菌落;支原体固体培养基上棕色油煎蛋状菌落边缘丝状;液体培养基上可鉴定为解脲脲原体。喹诺酮类、大观霉素耐药;大环内酯类、四环素类、林可霉素均敏感。后续靶向DNA测序结果一致也为解脲支原体。收到报告之前临床采用头孢曲松+甲硝唑常规经验抗感染覆盖阴性杆菌与厌氧菌,未对支原体进行针对应性治...     

解脲支原体保存法探讨

<正> 解脲支原体与一般细菌不同,形成菌落很小,且深埋在琼脂培养基里发育,不便采用白金耳从表面刮取菌落进行传代,支原体在4℃保存时,存活时间很短,给菌种保存带来了很多困难,本文对解脲支原体菌种冷藏、冷冻及冻干     

支原体双项药敏试验结果分析

目的 :了解支原体对多种抗生素的耐药性发展。方法 :对我院 2 0 0 1年 8月～ 2 0 0 3年 3月皮肤性病门诊病人 ,应用人型、解脲双项支原体药敏培养基 ,对 130株支原体对 10种抗生素的药敏监测 ,并对不同的菌株进行耐药性比较。结果 :螺旋霉素、壮观霉素、罗红霉素、环丙沙星、氧氟沙星、阿奇霉素、司怕沙星、对解脲、人型支原体和交叉感染的总耐药率分别为 6 3 1% ,4 7 7% ,4 0 8% ,38 5 % ,35 4 % ,33 1% ,2 9 2 % ,对解脲支原体敏感率仅在 7 6 %～2 8 6 %之间。结论 :强力霉素、美满霉素对人型、解脲和交叉感染的总敏感率分别为 91 5 %、93 1% ,可作为治疗支原体的首选药物。交沙霉素 5 9% ,仅对解脲支原体有效 ,对人型、解脲支原体引起的交叉感染无效     

支原体浓度与临床症状相关性研究

目的　探讨泌尿生殖道不同型支原体、支原体感染度与其临床症状关系。方法　采用法国梅里埃公司MycoplasmaID试剂盒 ,先后将分离出 10 35株支原体经 3次传代成纯培养物后接种于液体培养基 ,置烛缸 37℃培养 2 4～ 4 8h ,进行支原体鉴定、菌量计数并了解其临床表现。必要时可取 0 2ml培养物转种于固体培养基中 ,37℃2 4～ 72h后置低倍镜下观察 ,发现油煎蛋样菌落为人型支原体 (Mh)生长 ,呈棕黑色海胆状菌落为解脲支原体 (Uu)生长。结果　无论解脲支原体还是人型支原体女性感染率显著高于男性 ,分别为 80 0 3%、32 79% ;临床表现方面 ,女性症状明显轻于男性 ,有些甚至无症状。而男性大多数都有症状。结论　支原体浓度以Uu≥ 10 4ccu/ml为界 ,Uu≥ 10 4ccu/ml应被作为需要积极进行临床治疗的推荐指征 ;Uu <10 4ccu/ml临床上无任何症状者 ,可暂时不要给药 ,观察一段时间 ,以避免滥用抗菌素造成菌群失调和耐药菌株增多 ,对人型支原体无论Mh≥ 10 4ccu/ml和Mh <10 4ccu/ml都具有临床意义 ,尤其男性更需要及时采取治疗措施 ,以避免延误病情。     

解脲支原体的微量菌落形成单位和颜色变化单位的测定

脲支原体的定量计数,因其个体微小,一般需要在光学显微镜下计数平板上生长的菌落,得出菌落形成单位(CFU/ml)和在试管中观察液体培养基pH改变,依颜色变化程度确定颜色变化单位(CCU/ml)。本试验用40孔细胞培养板对解脲支原体进行微量CFU和CCU测定,一块细胞培养板相当于40支小试管和5～6块琼脂平板,但只需要1/150～1/10的常规培养基,经比较微量法与常规法差别不大。这种微量的方法容易做到定量规范化、大大减少了器材和培养基的消耗、缩短了其它的辅助工作量。微量法操作简便、误差小、重复性好,并能在同一块细胞培养板上对多份本进行培养和比较。     

解脲支原体检测试剂盒的研制

研制出一种快速、敏感、准确检测解脲支原体试剂盒。方法：筛选出促进解脲支原体生长的促 生长因子,替代新生牛血清。选用多种有效抑菌剂以省去固体培养基鉴定的步骤,并与Ｈａｙｆｌｉｃｋ培养基及无血培 养基进行对比研究。结果：７２小时自制试剂盒与Ｈａｙｆｌｉｃｋ培养基的检出符合率达９８．１％, １６小时内检出率自制 试剂盒、Ｈａｙｆｌｉｃｋ培养基、无血解脲支原体培养基分别为５２．６％、２７．６％、１６．３％,前者明显优于后两种培养基。结 论：自制解脲支原体试剂盒简便、快速、准确,是一种实用的检测试剂盒。     

嗜酸乳杆菌部分生理特性的研究

目的：对嗜酸乳杆菌生长曲线等生理特性进行研究,初步掌握该菌的生长、繁殖和代谢规律。方法：按国标GB/T4789.35-2003的方法测定菌落总数。以培养时间为横坐标,pH值、滴定酸度和菌落总数对数为纵坐标,绘制菌株（种）生长曲线。通过测定pH值及OD值,确定菌株（种）的最佳培养条件。通过测定活菌数,确定菌株（种）的接种方法及生长促进因子。结果：综合分析生长曲线,确定嗜酸乳杆菌在5%接种量,37℃下最佳收获期为12h～16h;最佳培养条件：初始pH值5.8,37℃培养;选择4种天然廉价物料（添加剂）作为菌株（种）的促生因子,发现对其生长均有促进作用,影响大小依次为：西红柿汁5%〉香菇汁3%〉胡萝卜汁5%〉啤酒5%〉对照。结论：得到嗜酸乳杆菌的最佳生长条件,为菌液培养、冻干粉制备及活菌制剂打下基础。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.