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??Objective    To observe whether the modified sandblasted??large-grit and acid-etched??SLA??surface titanium discs by the immobilized Arg-Gly-Asp??RGD??peptide via chemical grafting methods would promote the osseointegration in diabetic Guangxi BA-MA mini-pig models. Methods    The experiment was carried out in the Comparative Laboratory of General Hospital of Nanjing Military Region from June 2014 to October 2015. Five Guangxi BA-MA mini-pigs were used for this research. Three of them were randomly induced into diabetes models by using streptozotocin??STZ??as diabetes model??DM??group??and the other two pigs were set as non-diabetes model??NDM??group. RGD peptite was grafted on the SLA surface of titanium implant discs with chemical coupling method. Each mini-pig was implanted separately three SLA titanium discs and three RGD-SLA titanium discs??discs diameter = 5 mm??in maxilla. All animals were sacrificed at one month. Samples were fixed by 4% formaldehyde and analyzed by MicroCT and undecalcified histology methods. Results    During the observation period??there were no significant differences in the parameters of bone mineral density??BMD????bone trabecular thickness??Th.Th????trabecular number??Th.N??and trabecular spacing??Th.Sp??between the groups. Resin embedded tissue histological analysis showed that the bone contact ratio of NDM-RGD-SLA group??64.8%±18.4%??are significant higher than DM-RGD-SLA group??33.9%±11.7%????NDM-SLA group??39.5%±20.9%?? and DM-SLA group??36.4%±15.9%??. No significant differences were found when compared within other groups. Conclusion    RGD peptide modified SLA titanium discs obviously promoted bone to implant contact. Whether animals have diabetes or not??there is no significant effect on the osseointegration between titanium discs and the jaw bones. However??when modified by RGD peptide?? the SLA discs can obviously improve bone formation around implants in NDM animals??not in DM animals. It implies that diabetes may inhibit the osseointegration effects of RGD peptide.     

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??Objective??An animal model of condylar cartilage and subchondral bone injury during growing period is established??and three-dimensional measurement of mandibular was performed with Materialise Proplan CMF 3.0 software to investigate the effect of PRP on repairing condylar cartilage and maintaining mandibular growth in growing period. Methods??Thirty growing New Zealand white rabbits were randomly divided into three groups. The PRP group animals ??10?? were implanted in activated PRP after the establishment of the animal model of condylar cartilage and subchondral bone injury.The condylar cartilage and subchondral bone injury models were established in the operation group ??10??. The sham operation group ??10?? was similar to the operation group except for the condylar cartilage and subchondral bone injury. At 6 and 12 weeks after surgery the animals received spiral computed tomography??and SPCC data were collected.Maxillary and mandibular three-dimensional reconstruction was performed using Materialise Proplan CMF 3.0. The upper and lower jaws were separated and the morphology of condylar was completely revealed. The result of measurement are analysed with statistical method after the determining the marking points??reference planes and corresponding measurement indexes. Results??The indexes of the operation group were all smaller than those of the sham operation group and the PRP group at each time point??P < 0.05????6 weeks after surgery??the height of mandibular ramus??the length of condyle and the width of condyle in PRP group were less than those in sham operation group??P < 0.05??. There was no significant difference in mandibular length or condylar height between PRP group and sham operation group ??P > 0.05??. The mandibular ramus height and mandibular body length in PRP group were less than those in sham operation group at 12 weeks after operation??P < 0.05??. There was no significant difference in condylar height??length or width between PRP group and sham operation group ??P > 0.05??. Conclusion??After the injury of mandibular condylar cartilage??the growth and development of mandibular are relatively stagnant??and the mandibular condyle is absorbed. Activated PRP can effectively repair the injury of condylar cartilage during growing period??promote the normal growth and development of mandible and condyle??and maintain the normal shape of mandible.     

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目的研究抗菌肽RISE—AP12对体外培养的牙龈91、啉单胞菌（Porphyromonas gingivalis,P.g）的抗菌活性。方法本研究于2012年10月至2013年1月在中国医科大学附属口腔医院中心实验室进行。以P．g W83为实验菌株,采用麦氏比浊法配制P.g W83混悬液（10^6 CFU／mL）备用,微量液体稀释法测定抗菌肽RISE—AP12对P．g W83的最小抑菌浓度（minimal inhibitory concentration,MIC）和最小杀菌浓度（minimal bactericidal concentration,MBC）。结果抗菌肽RISE—AP12对10^6 CFU／mL P．g W83的MIC为0．01 mg／mL,MBC为0．02mg／mL。结论抗菌肽RISE—AP12对浮游状态下的P譬有抑菌（MIC为0,01 mg／mL）和杀菌（MBC为0．02 mg／mL）作用。     

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??Objective    To analyze the correlation between OSAHS children's minimal width measured in cephalometrical film and the minimal cross section area in CBCT to determine the accuracy of airway 2D measurement in the diagnosis of airway obstruction. Methods    48 OSAHS children??10-12y??diagnosed by PSG were selected. All the subjects took cephalometrical film and CBCT at the same time. We got the airway volume and minimum cross section area by inputing DICOM form data into the dolphin. Make the Pearson correlation analysis concerning the minimum width and minimum cross section area. Results    The minimal upper airway site measured in cephalometrical film was at the same place where the minimal cross section area lied. The mean value of the most narrow sagittal width was??3.79 ± 2.40??mm??the minimal cross section area measured in CBCT was??103.30 ± 34.47??mm2. Pearson correlation R = 0.670??P < 0.05??. Conclusion    2D and 3D upper airway measurement in OSAHS children has strong correction. Cephalometrical film can reflect the airway condition and can be used in the primary diagnosis of airway obstruction site and degree.     

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目的评价自体富血小板血浆应用于颌骨囊肿手术的临床疗效。方法将2007年8月至2008年8月浙江嘉兴医学院附属第二医院口腔科收治的颌骨囊肿患者30例随机分成试验组和对照组各15例。颌骨囊肿摘除后骨腔用含自体富血小板血浆的数字纱布充填为试验组,术后骨腔只用数字纱布充填为对照组。比较两组术后10d创口愈合情况和术后6个月骨缺损区骨密度的变化。结果术后10d创口软组织愈合和术后6个月骨缺损区骨密度的变化,试验组优于对照组。结论富血小板血浆局部应用能促进软硬组织的生长。     

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??Abstract??Objective To evaluate the the sealing effects and abrasive resistance of ClinproTM XT Varnish and Oravive through scanning electron microscope ??SEM??. Methods Totally 48 freshly extracted human premolars were selected?? prepared into dentin discs?? and randomly divided into 3 groups ??group A?? control group?? group B?? ClinproTM XT Varnish group??group C?? Oravive group??. ClinproTM XT Varnish and Oravive were applied respectively on the exposed dentin surfaces of the B and C group in accordance with operating manual. Eight teeth of the B and C group were respectively selected for toothbrush abrasion test. Then an observation was made on the surface and the section plane by scanning election microscope and quantitative analysis was made with Image-Pro Plus 6.0. Results Both of the two desensitizers could occlude the dentinal surface?? and dentinal tubule area and the relative area of the two groups had no significant difference ??P > 0.05??. The two desensitizers could penetrate into the dentinal tubules at a certain depth?? but the penetration depth in group B was larger than that in group C ??P < 0.05???? after the toothbrush abrasion test?? the sealing effect in group B was markedly superior to that in group C ??P < 0.05??. Conclusion ClinproTM XT Varnish performs better than Oravive in sealing effect and abrasion resistance.     

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目的比较镍铬、钴铬、纯钛、钯基、金铂等5种常用烤瓷合金的细胞毒性。方法本研究于2011年7—10月在福建医科大学附属口腔医院中心实验室进行。按照ISO10993标准,采用CCK-8法测定第1、3、5、7天时烤瓷合金浸提液培养的小鼠肺成纤维细胞(L929细胞)的OD值,同时观察L929细胞形态,并且计算细胞相对增殖率(RGR),评价5种烤瓷合金对L929的细胞毒性。结果第7天时,除金铂合金外,其他4组烤瓷合金的OD值与阴性对照组的差别均有统计学意义(P<0.05);L929细胞的RGR由高到低分别为金铂合金(98.4%)、钯基合金(95.2%)、纯钛合金(83.0%)、镍铬合金(74.0%)、钴铬合金(72.6%)。细胞形态学观察发现:金铂合金组、钯基合金组以及纯钛合金组的细胞形态与阴性对照组相比无明显差别;而钴铬合金组和镍铬合金组的细胞排列稀疏,少量细胞皱缩变圆。结论金铂合金、钯基合金以及纯钛合金的细胞毒性为1级,具有较好的生物相容性;钴铬合金、镍铬合金的细胞毒性为2级,结合细胞形态观察认为生物相容性仍然合格。     

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??Objective    To investigate the expression of miR-590 in the tissues of patients with oral lichen planus and oral squamous carcinoma and study its role in the carcinogenesis of oral mucosal cells. Methods    Twenty female and twelve male OLP patients??OLP group??and seventeen female and eleven male OSCC patients??OSCC group??were selected??who were pathologically diagnosed in Jinzhou Stomatological Hospital from March 2014 to December 2015. Twenty healthy matched volunteers served as control group. The expressions of miR-590 in oral tissues of the three groups were examined by realtime PCR. The difference among the three groups was statistically analyzed. Results    The relative expressions of miR-590 were significantly higher in OLP??1.96 ± 0.52??and OSCC patients??2.75 ± 0.78??than those in the healthy controls??0.77 ± 0.34????P < 0.05??. There were significant differences in the expression of miR-590 between the OLP group and OSCC group??P < 0.05??. Conclusion    The expressions of miR-590 are higher in OLP and OSCC patients than those in normal controls??miR-590 may play a role in the carcinogenesis of oral mucosal cells.     

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目的了解济南市小学生家长对口腔保健知识的认知程度,为济南市儿童口腔保健预防工作提供基线资料。方法 2009年1—5月对济南市10所小学2860名学生家长进行问卷调查,所得数据采用SPSS软件统计分析。结果家长对各种口腔保健知识的认知水平不同,在所调查的问题中,对刷牙的作用、频繁进食甜食饮料等易致龋坏等知识认知水平非常高,对六龄牙的重要性的认识也处于较高水平,但对于氟化物、窝沟封闭防龋、乳牙龋坏应及时治疗及咬合诱导知识等认知明显偏低,相关知识知晓率差异有统计学意义(P<0.05)。随家长文化水平及经济收入的提高,家长对部分口腔保健知识的掌握有明显提高(P<0.05)。不同年龄家长对部分口腔保健知识的掌握有明显差异(P<0.05)。结论济南市儿童家长口腔保健知识认知水平存在明显差异,部分家长口腔保健知识缺乏,保健意识不强,应加强针对性的口腔宣传与教育工作。     

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目的建立BALB／c近交系小鼠腭胚突间充质（EPM）细胞体外培养模型并研究其生物学特性。方法芩研究于2012年11月至2013年7月在山西医科大学医学寄生虫研究所进行。采用机械分离及胰蛋白酶消化法,简便、准确、快速地获得高成活率的BALB／c近交系小鼠EPM细胞,采用免疫组化法鉴定细胞,并进行细胞增殖和形态学观察。结果EPM细胞为成纤维细胞样细胞,呈梭型,单层漩涡状排列,排列无序,细胞核大,核分裂像较多。免疫组化染色显示,角蛋白标记为阴性,波形蛋白标记为阳性。EPM细胞呈指数生长,有较强的增殖能力。结论建立的BALB／c近交系小鼠EPM细胞体外培养技术模型可较好地保持EPM细胞的基本生物学特性。     

Maternal periodontitis and adverse pregnancy outcomes

Abstract – Objectives: Maternal periodontal diseases have been associated with increased risk of preterm birth and restricted fetal growth among relatively low socioeconomic groups. Whether the association can be generalized to middle‐class populations remains uncertain. We evaluated periodontitis in relation to preterm birth (<37 weeks’ gestation) and small‐for‐gestational‐age (SGA, birth weight below the 10th percentile of birth weight for gestational age) among a group of medically insured women. Methods: We conducted a prospective study among participants of Project Viva, a US cohort study of pregnant women and their offspring from 1999 to 2002. Pregnancy outcomes were obtained from medical records. Self‐reported periodontitis was assessed during the second trimester of pregnancy, and validated against radiographs. Logistic regression analyses were employed to evaluate the association of periodontitis with pregnancy outcomes adjusted for age, race/ethnicity, smoking status, income, frequency of dental check‐ups, prepregnancy body mass index, pregnancy weight gain, gravidity, prior history of preterm birth and history of genitourinary infection. Results: Of the 1635 women, 72.7% were Caucasian, 65.0% had annual household income >$70 000, 3.8% reported having periodontitis, 6.4% delivered preterm, 5.4% delivered SGA babies, and 11.0% had poor pregnancy outcome (either preterm birth or SGA). The odds ratio (OR) associated with periodontitis was 1.74 (95% CI 0.65–4.66) for preterm delivery and 2.11 (95% CI 0.76–5.86) for SGA individually. When preterm delivery and/or SGA were combined, the OR was 2.26 (95% CI 1.05–4.85) relating periodontitis with poor pregnancy outcome. Conclusion: Within the limitations of the study, the results suggest that periodontitis is an independent risk factor for poor pregnancy outcome among middle‐class women.     

牙周炎与不良妊娠结局相关关系的研究进展

牙周炎是由牙周致病菌感染引起的慢性炎症性疾病,在妊娠妇女中更容易发生。不良妊娠结局是新生儿围产期死亡的重要原因,受机体全身炎症的影响。该文主要对牙周炎与不良妊娠结局的相关性进行阐述,进一步从阴道感染、菌血症、免疫炎症和肠道菌群方面对其机制进行探讨,并简述了牙周炎与不良妊娠结局共有的宿主易感性遗传背景,以期为临床上预防妊娠期牙周病和不良妊娠结局提供一定的理论指导。     

牙龈卟啉单胞菌与不良妊娠结局的相关研究进展

龈卟啉单胞菌(Porphyromonas gingivalis, P.gingivalis)是一种革兰阴性厌氧菌,是牙周炎尤其是慢性牙周炎病变区或活动部位最主要的病原菌。研究表明,牙周炎与多种不良妊娠结局(adverse pregnancy outcomes, APO)有关,这一过程涉及多种病理生理机制,而P.gingivalis作为关键牙周致病菌之一在其中扮演重要角色。目前我们对P.gingivalis与APO的关系及其作用机制的认识仍然十分有限。该文拟通过回顾近年来发现的P.gingivalis与APO之间相关的直接或间接的证据,对P.gingivalis与APO的联系和作用机制作一综述。     

The relationship between the anti-Porphyromonas gingivalis immunoglobulin G subclass antibody and small for gestational age delivery: a longitudinal study in pregnant Japanese women

Background: Maternal serum IgG antibody against Porphyromonas gingivalis is an indicator of both periodontitis and adverse pregnancy outcomes. This study aims to evaluate the anti-P. gingivalis IgG and IgG subclasses1–4 in threatened preterm labour (TPL) patients and their association with small for gestational age (SGA). Methods: Serum, saliva and subgingival plaque samples were collected from 47 TPL patients compared with 48 healthy pregnant women. The amount of P. gingivalis was measured in saliva and plaque using real-time polymerase chain reaction. The serum anti-P. gingivalis IgG titre and anti-P. gingivalis subclasses IgG 1–4 concentration were measured using enzyme-linked immunosorbent assay. Results: The amount of anti-P. gingivalis IgG-1 was significantly lower in the TPL group than in the healthy group. Fourteen subjects delivered SGA infants in the TPL group. The pocket probing depth (PPD), clinical attachment loss, PPD ≥ 5 mm%, amount of P. gingivalis in plaque, anti-P. gingivalis IgG and anti-P. gingivalis IgG-4 were significantly higher in the TPL-SGA group than in the TPL-normal weight group. Moreover, logistic regression analysis revealed the detection frequency of P. gingivalis in plaque and placenta weight were significantly correlated with SGA in TPL. In the receiver operating characteristic curve analysis, an amount of P. gingivalis in plaque ≥ 86.45 copies showed a sensitivity of 0.786 and a specificity of 0.727 (AUC 0.792) for predicting SGA in TPL. Conclusion: Lower anti-P. gingivalis IgG-1 amounts are related to TPL, while higher anti-P. gingivalis IgG and IgG-4 are related with SGA in TPL. Further, greater colonisation of P. gingivalis in plaque might increase the risk of SGA and can be useful in prediction of SGA in TPL.Key words: Porphyromonas gingivalis, immunoglobulin G subclasses antibody, threatened preterm labour, small for gestational age     

牙周炎和胎膜早破的关系及其炎症介质的研究进展

牙周炎是一种慢性感染性疾病,通常伴有局部或全身的炎症介质变化或免疫环境紊乱,在临床上与多种全身性疾病密切相关.胎膜早破是一种以羊膜内感染为主要因素的疾病,最终以胎膜胶原蛋白破坏,胎膜弱化破裂为结局.近年来,研究者们发现牙周炎和胎膜早破存在一定的相关性,但其中具体的相关机制尚不清楚.该文搜集资料并归纳了与二者相关的多种因...     

The relationship between infections and adverse pregnancy outcomes: an overview

Preterm birth with its subsequent morbidity and mortality is the leading perinatal problem in the United States. Infants born before the thirty-seventh week of gestation account for approximately 6% to 9% of all births, but 70% of all perinatal deaths and half of all long-term neurologic morbidity. Current approaches focus on symptomatic treatment. Despite widespread use of drugs to arrest preterm labor (tocolytics), there has been no decrease in low birth weight or preterm infants in the last 20 years. It is likely that therapy directed at preventing or treating underlying causes would be more successful. Evidence from many sources links preterm birth to symptomatic infections, for example, of the urinary or respiratory tracts. In the last decade, great interest has been generated to support the hypothesis that subclinical infection is an important cause of preterm labor. Evidence to support this may be categorized as follows: histological chorioamnionitis is increased in preterm births; clinical infection is increased after preterm birth; there is significant association of some lower genital tract organisms and infections with preterm birth or preterm premature rupture of the membranes; there are positive cultures of amniotic fluid or membranes from some patients with preterm labor and preterm birth; there are markers of infections in preterm birth; bacteria or their products induce preterm birth in animal models; and some antibiotic trials have shown a lower rate of preterm birth or have deferred preterm birth. In the last 5 years, additional exciting information has suggested that not only is subclinical infection responsible for preterm birth but also many serious neonatal sequelae including periventricular leukomalacia, cerebral palsy, respiratory distress, and even bronchopulmonary dysplasia and necrotizing enterocolitis. In sum, a large body of clinical and laboratory information suggests that subclinical infection is a major cause of preterm birth, especially those occurring before 30 weeks. This concept holds promise that new approaches can be developed to prevent prematurity.     

The relationship between maternal periodontitis, adverse pregnancy outcome and miscarriage in never smokers

BACKGROUND: It has been postulated that associations between periodontal disease and systemic conditions may be because of the confounding effects of smoking. In addition, studies of this type rarely investigate the adverse pregnancy outcome of miscarriage. AIM: The aim of this prospective study was to investigate a relationship between periodontal disease in pregnancy and subsequent adverse pregnancy outcomes in a population of never smokers. MATERIALS AND METHODS: Pregnant women were recruited at 12 weeks gestation. Demographic, behavioural and medical data were collected. A periodontal examination was performed and data on each subjects' pregnancy outcome were collected. RESULTS: A total of 1793 women reported never previously smoking. Of these, 7.3% had a pre-term birth and 0.9% a late miscarriage. As expected in this population, we found no associations between poorer periodontal health and either pre-term birth or low birth weight (LBW). In contrast, the subjects who experienced a late miscarriage had a higher mean probing depth at mesial sites compared with the subjects that gave birth at term (2.69 mm versus 2.41 mm, p=0.006). CONCLUSIONS: There was an association between some measures of periodontal disease and late miscarriage; however, there was no association between periodontitis and pre-term birth or LBW in this population.     

固齿散治疗牙周炎的II期临床试验总结

目的 :评价固齿散对牙周炎的临床疗效及其安全性 ,进一步明确固齿散的作用。方法 :采用随机单盲对照试验方法治疗牙周炎 131例。以固齿散为治疗组 ,碳酸钙粉剂为对照组 ;治疗组 10 0例 ,对照组31例。结果 :治疗组与对照组的显效率分别为 2 0 .2 5 %和 3.2 3% ,临床总有效率分别为 82 .2 5 %和 6 4 .5 2 % ,两组比较 ,差异有显著性意义 (P <0 .0 5 )。固齿散具有良好的消炎、止血等作用。WBC、HB、GPT、BUN、BSR等各项检查均未能提示固齿散对患者有明显的毒副作用和不良反应。结论 :固齿散治疗牙周炎的临床疗效好 ,无毒副作用和不良反应