鼠神经生长因子用于视神经挫伤的临床疗效

目的：观察注射用鼠神经生长因子对视神经挫伤的临床疗效。方法：选择的118例（118眼）视神经挫伤患者,并将其随机分成治疗组59例（59眼）,对照组59例（59眼）。治疗组在对照组的基础上给予注射用鼠神经生长因子18μg 肌肉注射,1次／d,疗程4周。对照组给予大剂量激素冲击治疗及综合治疗。随访6个月～1年观察注射用鼠神经生长因子的疗效。结果：治疗组的有效率为83.1％,对照组的有效率为52.5％,两组比较差异有显著性（P ＜0.05）。结论：注射用鼠神经生长因子治疗视神经挫伤有显著疗效.可促进视功能的恢复,提高视力：     

注射用鼠神经生长因子治疗视神经挫伤的疗效观察

目的：观察注射用鼠神经生长因子对视神经挫伤的临床疗效。方法：选择的46例（46眼）视神经挫伤患者,并将其随机分成治疗纽23例（23眼）,对照纽23例（23眼）。治疗组在对照纽的基础上给予注射用鼠神经生长因子301xg肌肉注射,1次／d,疗程4周,部分病例6周。对照组给予大剂量激素冲击治疗及综合治疗。随访6个月～1年观察注射用鼠神经生长因子的疗效。结果：治疗组的有效率为78．26％,对照组的有效率为52．17％,两组比较差异有显著性（X2=6．757,P=0．034）。结论：注射用鼠神经生长因子治疗视神经挫伤有显著疗效,可促进视功能的恢复。提高视力。     

神经生长因子结合高压氧治疗脊髓损伤疗效分析

目的探讨神经生长因子（NGF）结合高压氧（HBO）治疗脊髓损伤（SCI）的疗效。方法182例SCI患者随按治疗方法的不同分为NGF联合HBO组（NGF-I--IBO组）和常规药物治疗组（常规组）,NGF-HBO组予鼠NGF结合HBO治疗,对照组除不应用NGF及HBO治疗外,其余治疗与治疗组相同。结果治疗1个疗程后,NGF-HBO组126例中显效例78例,有效39例,无效9例,总有效率92．9％（117／126）。对照组56例中,显效13例,有效21例,无效22例,总有效率60．7％（34／56）。两组差异有统计学意义（P〈0．01）。结论NGF结合HBO对脊髓损伤疗效肯定,优于常规治疗。     

鼠神经生长因子治疗后天麻痹性斜视疗效观察

观察鼠神经生长因子治疗后天麻痹性斜视的临床疗效。方法将82 例后天麻痹性斜视患者随机分为鼠神经生长因子治疗组（治疗组）41 例和常规治疗组（对照组）41 例。对照组甲钴胺片口服0.5 mg,3 次/d,维生素B1 口服10 mg,3 次/d,治疗组加用肌肉注射鼠神经生长因子18μg,1 次/d,14 d为1 个疗程,治疗3 个疗程后观察疗效。结果痊愈26 例（63.41%）,有效10 例（24.39%）,无效5 例（12.20%）,总有效率87.80%;对照组痊愈13 眼（31.71%）,有效17 眼（41.46%）,无效11 眼（26.83%）,总有效率73.17%,两组疗效差异有统计学意义（ z=-2.807, p<0.05）,且治疗组三棱镜斜视度改善程度优于对照组,差异有统计学意（p <0.05）。结论鼠神经生长因子治疗后天麻痹性斜视能提高临床疗效,改善症状,减轻斜视度,值得推广。     

神经生长因子治疗脑出血临床疗效观察

目的：观察注射用鼠神经生长因子治疗脑出血的临床疗效。方法：选取脑出血患者78例,随机分为2组,对照组39例,应用胞二磷胆碱注射液0.5g,静滴,1次/d,观察组39例,应用注射用鼠神经生长因子18μg,肌内注射,1次/d,疗程4周,分别于治疗前及治疗后4周,对患者进行神经功能缺损评分及临床疗效评分。结果：治疗后,2组患者神经功能缺损评分较治疗前明显降低,差异有统计学意义（P＜0.05）。治疗后4周,观察组神经功能缺损评分较对照组明显降低,临床有效率明显增高,差异均有统计学意义（P＜0.05）。结论：注射用鼠神经生长因子可有效缓解脑出血患者的神经功能缺损。     

鼠神经生长因子对恢复期早中期面神经炎的疗效分析

目的 探讨鼠神经生长因子(mNGF)治疗恢复期早中期面神经炎患者的疗效.方法 方便选取2012年8月—2016年8月期间在该院门诊或住院治疗的恢复期早中期面神经炎患者118例为研究对象.根据治疗方法分为应用鼠神经生长因子组(mNGF治疗组)及无应用鼠神经生长因子的对照组,两组的其余治疗方案相同,对比观察两组的有效率和治愈率.结果 mNGF治疗组的有效率96.5％、治愈率44.8％;对照组的有效率83.3％、治愈率26.7％.mNGF治疗组的疗效好于对照组,差异有统计学意义(P<0.05).结论 鼠神经生长因子对恢复期早中期面神经炎的患者有疗效,值得推荐.     

注射用小牛血去蛋白提取物治疗急性脑出血30例疗效观察

目的:观察注射用小牛血去蛋白提取物治疗急性脑出血的临床疗效。方法:60例急性脑出血患者随机分为两组,每组30例,对照组使用胞二磷胆碱1.0 g,每日1次;治疗组在对照组的基础上使用注射用小牛血去蛋白提取物1 200mg,每日1次,两组均14 d为1个疗程。结果:治疗组治疗后显效11例,有效3例,无效1例,总有效率为90.1%;对照组治疗后显效7例,有效4例,无效4例,总有效率为73.33%。治疗后两组总有效率比较差异有统计学意义(P<0.05)。结论:注射用小牛血去蛋白提取物治疗急性脑出血疗效确切,安全性高。     

鼠神经生长因子对老年带状疱疹后遗神经痛病人的疗效评价

目的:探讨鼠神经生长因子（mNGF）治疗老年带状疱疹后遗神经痛（PHN）病人的临床疗效。方法:将老年PHN病人50例,随机分成观察组和对照组,各25例,对照组予维生素B₁₂ 500 μg,每天1次;观察组予mNGF 20 μg肌内注射,每天1次;2组疗程均14 d。2组病人治疗前后,均采用疼痛视觉模拟评分法（VAS）进行评分,评估疗效,并记录不良反应。结果:观察组VAS评分显著低于对照组（P<0.01）,总有效率、疼痛减轻程度和疼痛消失时间均明显优于对照组（P<0.01）。结论:采用mNGF治疗PHN安全有效。     

注射用鼠神经生长因子治疗重症病毒性脑炎疗效观察

目的：观察注射用鼠神经生长因子治疗重症病毒性脑炎的疗效。方法：将治疗组18例重症病毒性脑炎与回顾性重症病毒性脑炎对照组15例相比较,对照组用抗病毒、脱水剂、降颅压、营养脑细胞及丙种球蛋白治疗。治疗组则在对照组治疗的基础上加用注射用鼠神经生长因子治疗。结果：经统计学处理治疗组和对照组有显著差异（P〈0.05）。结论：注射用鼠神经生长因子对重症病毒性脑炎临床症状的恢复非常重要。     

注射用鼠神经生长因子联合神经节苷脂治疗脑出血后昏迷效果观察

目的:观察注射用鼠神经生长因子联合神经节苷脂治疗脑出血后昏迷患者的效果。方法:选取63例脑出血后昏迷患者作为研究对象,按照随机数字表法将其分为对照组31例和观察组32例,两组均给予常规治疗,对照组采用纳洛酮和神经节苷脂治疗,观察组在对照组的基础上给予注射用鼠神经生长因子治疗,比较两组临床疗效、治疗前后脑血肿体积及日常生活能力、不良反应发生情况。结果:观察组的治疗有效率为93.75%(30/32),明显高于对照组的70.97%(22/31),差异有统计学意义(P<0.05);治疗后观察组脑血肿体积小于对照组,差异有统计学意义(P<0.05);两组不良反应发生率比较,差异无统计学意义(P>0.05);治疗后观察组Barthel指数评分明显高于对照组,差异有统计学意义(P<0.05)。结论:神经节苷脂联合注射用鼠神经生长因子可减少脑出血后昏迷患者脑血肿体积,提高疗效,提高患者日常生活能力,且安全性高。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.