慢性肾小球肾炎患者血清中氧自由基对肾损害的探讨

测定慢性肾小球肾炎患者红细胞铜、锌超氧化物歧化酶（Ｃｕ，－ＳＯＤ）及血清过氧化脂质荧光参数（ＬＰＯ）的改变，结果表明慢性肾小球肾炎患者Ｃｕ，Ｚｎ－ＳＯＤ活力降低，血中ＬＰＯ增高；且随血肌酐的增高或内生肌酐清除率的降低，Ｃｕ，Ｚｎ－ＳＯＤ活力明显降低，ＬＰＯ增高。提示氧自由基对肾脏具有损伤作用，增加Ｃｕ，Ｚｎ－ＳＯＤ及活力可能是治疗慢性肾小球肾炎的重要措施之一。     

肾脏病患者血浆过氧化脂质含量和血清超氧化物歧化酶含量的观察

测定了５７例肾脏疾病和４７例正常人的血浆过氧化脂质和血清超氧化歧化酶（ＳＯＤ）含量，结果显示慢性肾盂肾炎患者血浆ＬＰＯ均值显著高于正常（Ｐ〈０．０５），而血清ＳＯＤ显著低正常（Ｐ〉０．０５）；肾病综合征Ⅱ型的ＬＰＯ均显著高于正常（Ｐ〈０．００１），肾病综合征Ⅰ型的ＬＰＯ与正常人相比无显著性差异（Ｐ〉０．０５）；肾病综合Ⅰ型和Ⅱ型患者血清ＳＯＤ与正常人相比无显著性差异（Ｐ〈０．０５）；慢性肾功能不全     

三七总皂苷对冠心病患者过氧化脂质及纤维蛋白溶酶原激活物的影响

目的：观察三七总皂苷对冠心病患者血清超氧化物歧化酶（ＳＯＤ）、过氧化脂质（ＬＰＯ）、组织型纤维蛋白溶酶原激活物（ｔＰＡ）及组织型纤维蛋白溶酶原激活物抑制剂（ＰＡＩ－１）的影响。方法：将４５例患者分成２组,三七总皂苷治疗组用三七总皂苷注射液５００ｍｇ加入２５０ｍｌ液体中静滴,ｑｄ,共１周,余治疗同对照组。结果：治疗组血清ＳＯＤ活力明显增高（Ｐ〈０．０１）,ＬＰＯ含量降低（Ｐ〈０．０１）。对照组ＳＯＤ活力及ＬＰＯ含量用药前后无明显改变（Ｐ均〉０．０５）,血清ＳＯＤ的含量在治疗组用药前后和对照组皆无明显差别（Ｐ均〉０．０５）。三七总皂苷治疗后ｔＰＡ明显高于治疗组,ＰＡＩ－１明显低于治疗前（Ｐ均〈０．０１）。而对照组治疗前后无明显变化（Ｐ均〉０．０５）。结论：三七总皂苷能升高冠心病患者ＳＯＤ活力,降低ＬＰＯ含量,提高纤     

血浆丙二醛及红细胞超氧化物歧化酶测定与呼吸系统疾病的关系

对呼吸系统疾病患者的血浆丙二醛（Ｐ－ＭＤＡ）含量及红细胞超氧化物歧化酶活性（ＲＢＣ－ＳＯＤＡ），进行了测定，并与对照组比较。结果显示：肺心病急性加重期与重度肺结核患者Ｐ－ＭＤＡ含量高于对照组（Ｐ〈０．０１），ＲＢＣ－ＤＯＳＡ明显低于对照组（Ｐ〈０．０１）；肺癌与轻、中度肺结核患者Ｐ－ＭＤＡ含量及ＲＢＣ－ＳＯＤＡ与对照组比较差异无显著意义（Ｐ〉０．０５）。表明肺心病急性加重期与重度肺结核患者自由基的     

对妊高征患者红细胞膜脂质过氧化和离子运输功能的观察

目的：对妊高征患者红细胞膜过氧化脂质（ＬＰＯ）含量、超氧化物歧化酶（ＳＯＤ）活性、Ｎａ＾＋－Ｋ＾＋－ＡＴＰ酶活性及膜内面Ｃａ＾２＋结合能力的变化进行研究。方法：检测１９名正常妇孕,１９例妊高征患者红细胞膜ＬＰＯ、ＳＯＤ、Ｎａ＾＋－Ｋ＾＋－ＡＴＰ酶活性（比色法）及膜内面Ｃａ＾２＋结合力（原子吸收法）。结果：（１）妊高征组红细胞膜ＬＰＯ含量明显高于正常孕妇组（Ｐ〈０．００１）。（２）妊高征组红细胞膜Ｓ     

VE,VC联合佐治新生儿缺氧缺血性脑病及其对脂质过氧化物和超氧化？…

应用自由基清除剂维生素Ｅ（ＶＥ）、维生素Ｃ（ＶＣ）联合佐治新生儿缺氧缺血性脑病（ＨＩＥ），观察其疗效和治疗前后患儿血清中脂质过氧化物（ＬＰＯ）和超氧化物歧化酶（ＳＯＤ）含量的变化，并同时与对照组比较。其结果显示：治疗组的显效率高于对照组（Ｐ〈０．０５）；在治前，两组血清ＳＯＤ与ＬＰＯ含量比较均无差异（Ｐ〈０．０５），在治后，治疗组ＳＯＤ明显增加，ＬＰＯ明显降低，与对照组比较均有显著性差异（Ｐ〈０．     

海脉冲营养素抗衰老作用的实验研究

本文报道了海脉冲营养素对老龄大鼠体内过氧化脂质（ＬＰＯ）,脂褐素（ＬＰＦ）的含量和超氧化物歧化酶（ＳＯＤ）,光胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）活性的影响。给老龄大鼠灌服海脉冲营养６０ｄ,结果表明：血液及肝脏中ＬＰＯ含量明显降低（Ｐ〈０．０５）;ＳＯＤ和ＧＳＨ－Ｐｘ酶活性显著提高（Ｐ〈０．０５）;大脑皮层中ＬＰＦ的生成明显减少（Ｐ〈０．０１）。提示海脉冲营养素具有提高老龄大鼠体内抗氧化酶活性、清除自由基、抑制脂质过氧化的作用。     

纳络酮对缺血再灌注心肌脂质过氧化物和钙含量的影响

利用犬心肌缺血，再灌注模型，观察了纳络酮对缺血再灌注心肌脂质过氧化物（ＬＰＯ），超氧化物岐化酶（ＳＯＤ）和钙含量的影响，结果表明，缺血，再灌注心肌ＬＰＯ和钙含量明显增加（Ｐ〈０．０５，Ｐ〈０．０１）。再灌注心肌ＬＰＯ含量和钙含量明显高于缺血心肌（Ｐ〈０．０５，Ｐ〈０．０１），而缺血，再灌注心肌ＳＯＤ明显降低（Ｐ〈０．０１）；纳络酮明显降低缺血，再灌注心肌ＬＰＯ和钙含量（Ｐ〈０．０５，Ｐ〈０．０１）     

聚乙二醇修饰超氧化物歧化酶的研究

以氰尿酰氯活化单甲氧基聚乙二醇（ＭＰＥＧ Ｍｒ５０００）对猪血Ｃｕ，Ｚｎ－ＳＯＤ进行修饰，制得ＭＰＥＧ２－ＳＯＤ。产物的酶活性保持８２．０％，期半衰期比修饰前延长至７．６倍，在动物脑中的ＳＯＤ活性较高。修饰ＳＯＤ抗炎活性增强，抗原性降低，稳定性提高。     

香烟依赖与血浆LPO含量及红细胞SOD活性关系的研究

检测经配对设计的５７例吸烟者和５７例健康非吸烟者的血浆过氧化脂质（Ｐ－ＬＰＯ）含量以及红细胞超氧化物歧化酶活性（Ｅ－ＳＯＤＡ）的结果表明，与健康非吸烟组比较．吸烟组的Ｐ－ＬＰＯ平均含量显著升高（Ｐ＜０．００１）、Ｅ—ＳＯＤＡ平均值显著降低（Ｐ＜０．００１），吸烟者的Ｐ－ＬＰＯ含量随吸烟者吸烟史和吸烟量的增加而升高、Ｅ－ＳＯＤＡ值随吸烟者吸烟史和吸烟量的增加而降低，并均呈线性相关（Ｐ＜０．００１）；提示吸烟者体内的氧自由基反应及脂质过氧化反应明显加剧。     

肾上腺皮质疾病患者超氧化物歧化酶和丙二醛的表达及意义

目的 探讨超氧化物歧化酶活性（SOD）和丙二醛水平（MDA）在肾上腺皮质疾病患者外周血血清和组织中的表达及与意义. 方法 选择肾上腺皮质增生患者12例、肾上腺皮质腺瘤患者10例,健康对照者11例,分别比较对照组和病例组患者术前血清学铜/锌超氧化物歧化酶（Cu/Zn-SOD）、锰超氧化物歧化酶（Mn-SOD）活性和MDA含量、肾上腺组织免疫组化染色Cu/Zn-SOD、Mn-SOD阳性细胞占细胞总数百分比之间的差异. 结果 病例组各组患者术前血清中Cu/Zn-SOD、Mn-SOD活性显著低于健康对照组（P＜0.05）,MDA含量显著高于健康对照组（P＜0.05）;Cu/Zn-SOD染色面积在肾上腺皮质腺瘤组中表达显著降低（P＜0.05）,Mn-SOD染色面积在肾上腺皮质增生和肾上腺皮质腺瘤组中表达显著降低;SOD的血清学与组织学之间表达呈正相关（P＜0.05）. 结论 SOD和MDA在肾上腺皮质疾病患者血清和组织中表达异常,联合检测SOD、MDA可能为肾上腺皮质疾病的鉴别诊断提供新的方法.     

Ethanol fraction of Aralia elata Seemann enhances antioxidant activity and lowers serum lipids in rats when administered with benzo(a)pyrene

Aralia elata Seemann is an edible mountain vegetable in Korea containing saponin, alkaloid, palmitic acid, linoleic acid, methyl eicosanoate and hexacosol, and is known to manifest an effect on cardiac infarction, gastric ulcer, colitis, and enervation. This study has examined the effects of Aralia elata Seemann ethanol extract on antioxidant enzyme systems and lipid metabolism in rats along with benzo(a)pyrene (B(a)P) administration. Rats were divided into four groups: control (C), an extract fed group (CE), a B(a)P fed group (CB), and a B(a)P and extract fed group (CBE). The ethanol extracts of Aralia elata Seemann (50 mg/kg body weight) were fed to the rats for 4 weeks by stomach tubing. Extract administration increased the antioxidant activities of glutathione sulfur transferase (GST). Total superoxide dismutase (SOD) and Cu,Zn-SOD activities were stimulated. Catalase activities were increased by 50% with extract feeding. Cu,Zn-SOD was greatly enhanced from 0.10 unit to 0.18 unit and catalase activity also was increased. Serum alpha-tocopherol was markedly increased by the extracts. The ethanol fraction of Aralia elata Seemann decreased total serum cholesterol. However, serum HDL-cholesterol was increased by 35% (p<0.05). The results indicate that Aralia elata Seemann exerts antioxidant and strong hypocholesterolemic and hypolipidemic effects in vivo with the administration of B(a)P.     

PGⅠ、PGⅡ的水平在胃癌诊断中的意义

目的探讨血清中胃蛋白酶原(PGⅠ、PGⅡ)含量及其比值(PGR),在胃癌诊断中的意义。方法采用时间分辨荧光免疫分析法(TRFIA),检测198例正常对照者、89例胃溃疡患者及67例胃癌患者血清中的PGⅠ、PGⅡ含量并计算PGR。结果胃溃疡患者PGⅠ、PGⅡ较正常对照组明显升高,差异具有显著性(P<0.05),PGR差异无显著性(P>0.05);胃癌患者PGⅠ、PGR较正常对照组显著降低,差异显著(P<0.01),PGⅡ差异无显著性(P>0.05)。结论测定血清PGⅠ、PGⅡ水平及PGR值对胃癌的早期筛查及鉴别诊断有临床意义。     

肺癌、肺结核患者血中LPO、SOD测定的临床意义

目的了解SOD、LPO在肺癌患者及肺结核患者血中的水平文化。方法随机选择肺癌患者 2 8例 ,肺结核患者 6 7例 ,正常对照 35例 ,检测血中SOD及LPO并进行比较分析。结果肺癌患者血SOD水平较正常对照明显下降 (P <0 0 1 ) ,而LPO水平则较正常对照组增高 (P <0 0 5 ) ;肺癌各组类型间血中SOD、LPO水平无显著性差异 (P >0 0 5 ) ;肺结核组患者血中SOD、LPO均较正常对照组明显增高 (P <0 0 5 )。结论肺癌患者血中SOD下降、LPO增高 ,可能对机体造成一定损害 ,肺结核血中SOD、LPO增高 ,似对机体有保护作用     

幽门螺杆菌相关性胃疾病血清PG测定及意义

为研究血清胃蛋白酶原 (pepsinogen,PG)亚群 (PG 、PG )含量与幽门螺杆菌 (Helicobacter pylori,Hp)相关性胃疾病的关系 ,选取体检健康者 3 0例 (对照组 )和疾病组 14 4例 (实验组 ) ,其中慢性浅表性胃炎 47例 ,慢性萎缩性胃炎 3 5例 ,胃溃疡 40例 ,胃癌 2 2例。采用快速尿素酶试验 (RUT)及病理切片法检测幽门螺杆菌感染 ,采用放射免疫法 (RIA)检测血清胃蛋白酶原含量 ,旨在研究胃相关性疾病患者以上指标的变化情况。结果显示 ,RUT法检测幽门螺杆菌阳性率 (79.17% )高于病理切片法 (4 0 .2 8% ) ,两者有显著性差异 (P<0 .0 1) ;幽门螺杆菌在慢性浅表性胃炎、慢性萎缩性胃炎及胃溃疡患者中有较高的阳性率 ,胃癌组患者 Hp感染率低于胃炎及胃溃疡组 ;在血清 PG含量检测中 ,胃癌组低于对照组、浅表性胃炎组及胃溃疡组 (P<0 .0 1) ,胃癌组 PG /PG 比值低于对照组及浅表性胃炎组 (P<0 .0 1) ,Hp(+ )组 PG 、PG 含量均高于 Hp(-)组。     

幽门螺旋杆菌根除对幽门螺旋杆菌相关性胃病患者血清COX-2影响

目的 探讨幽门螺旋杆菌(Hp)根除对幽门螺旋杆菌相关性胃病患者血清COX-2影响.方法 将2014年7月至2016年1月本医院收治的310例Hp相关性胃病患者按照病理学诊断结果分为胃溃疡组117例、十二指肠溃疡组96例和慢性萎缩性胃炎组97例,另选择同期在医院体检的健康人群60例作为对照组,所有感染Hp患者均行标准四联疗法,记录胃病患者Hp根除率;分别抽取各组受试对象肘静脉血,采用酶联免疫吸附试验检测四组血清环氧合酶-2(Cyclooxygenase-2,COX-2)水平.结果 310例胃病患者Hp根除273例,总根除成功率88.1％ (273/310);胃溃疡组、十二指肠溃疡组Hp根除成功率比较差异无统计学意义(x 2=2.375,P＞0.05),胃溃疡组、十二指肠溃疡组Hp根除成功率显著高于慢性萎缩性胃炎组(x2=3.996、9.162,P＜0.05).胃溃疡组、十二指肠溃疡组、慢性萎缩性胃炎组血清COX-2显著高于对照组(P＜0.05);胃溃疡组、十二指肠溃疡组血清COX-2又显著高于慢性萎缩性胃炎组(P＜0.05),胃溃疡组、十二指肠溃疡组两组间COX-2水平比较差异无统计学意义(P＞0.05).Hp根除成功的胃溃疡组、十二指肠溃疡组、慢性萎缩性胃炎组患者治疗后血清COX-2显著降低,与治疗前比较差异具有统计学意义(P＜0.05);Hp根除失败的胃溃疡组、十二指肠溃疡组、慢性萎缩性胃炎组患者治疗前后血清COX-2比较差异均无统计学意义(P＞0.05).结论 Hp相关性胃病患者在成功根除Hp后,血清COX-2会显著降低,血清COX-2可以作为Hp根除疗效评价指标之一.     

Cu2+、Zn2+胁迫对蛹虫草(Cordyceps militaris) 抗氧化酶活性及脂氧化水平的影响

研究了Cu^2＋、Zn^2＋对蛹虫草菌丝体抗氧化酶活性及脂氧化水平的影响。结果表明：高浓度的Cu^2＋抑制菌丝体的生长和细胞内抗氧化酶的活性；高浓度的Zn^2＋对菌丝体的生长也有抑制作用但抗氧化酶的活性变化不大。脂氧化水平随细胞内过氧化状态的变化而变化。蛹虫草菌丝体对Cu^2＋、Zn^2＋有较高的耐受度。     

Effect of intermittent administration of omeprazole on serum pepsinogens in duodenal ulcer patients and healthy volunteers.

1. Omeprazole, a substituted benzimidazole inhibitor of the gastric H+/K(+)-APT-ase, was administered orally at a dose of 20 mg in the morning of 3 consecutive days, followed by a period of 4 days without medication, and this intermittent dosage regimen was continued for 4 weeks. 2. During intermittent administration of omeprazole to 10 patients with duodenal ulcer disease and 10 healthy volunteers concentrations of serum pepsinogen A and serum pepsinogen C were monitored by sensitive and specific radioimmunoassays to study whether the effect of this treatment on serum pepsinogens is different between patients and normal subjects and to evaluate whether serum pepsinogen levels can be used to assess compliance with therapy. 3. Administration of omeprazole for 3 days induced significant increases in pepsinogen A and pepsinogen C serum concentrations, which rapidly fell after stopping the omeprazole intake. The pattern of serum pepsinogens after stopping the drug was different for duodenal ulcer patients and normal subjects. Both pepsinogens were intra-individually related in both patients and healthy subjects when compared during the first and last 3-day course with omeprazole, but in duodenal ulcer patients both pepsinogens tended to be higher in the last treatment course, while the opposite was found in the normal subjects. 4. The present study confirms that serum pepsinogen concentrations are higher in duodenal ulcer patients than in normal subjects, but also shows for the first time that serum pepsinogens in the patients respond differently upon stimulation with omeprazole.(ABSTRACT TRUNCATED AT 250 WORDS)     

817例儿童血红细胞SOD活性及血清LPO含量正常值研究

用邻苯三酚法及简易荧光法测定了817例儿童正常血红细胞内SOD活性及血清过氧化脂质含量。结果显示:(1)脐带血红细胞SOD活性显著低于各年龄组正常儿童(P<0.05),血清LPO含量显著高于(P<0.05)各年龄组正常儿童。(2)围产期血红细胞SOD活性明显低于新生儿围产期儿以后的各组儿童(P<0.05),血清LPO含量明显高于新生儿围产期儿以后的各组儿童(P<0.05)。(3)7天后新生儿血红细胞SOD活性明显低于其它各组儿童(P<0.05),血清LPO含量明显高于其它各组儿童(P<0.05)。(4)红细胞内SOD活性随年龄增长而逐渐升高。(5)7～14岁年龄组段内血清LPO含量女低于男(P<0.01)。(6)围产期新生儿组新生儿足跟血与静脉血红细胞内SOD活性、血清LPO含量无显著性差异(P>0.05)。     

Salivary immunoreactive endothelin in patients with upper gastrointestinal diseases

Endothelins have been implicated in gastric mucosal damage in a variety of animal models. Furthermore, clinical reports also show elevated gastric mucosal endothelin-1 levels in patients suffering from peptic ulcer diseases. We have demonstrated, first, the presence of immunoreactive endothelin (IR-ET) in human saliva. We also show that endothelins are rather stable in human saliva. The present study was undertaken to determine whether patients with endoscopically proven upper gastrointestinal diseases have a salivary excess of IR-ET, compared with patients with a normal esophagogastroduodenoscopy. Saliva was collected from fasting subjects prior to esophagogastroduodenoscopy. The levels of IR-ET were measured by the radioimmunoassay method. The salivary concentrations of IR-ET in the studied subjects were as follows: 8.9 +/- 1.0 fmol/mL (mean +/- standard error of the mean) for patients with gastric ulcers (n = 18); 7.3 +/- 1.0 fmol/mL for patients with duodenal ulcers (n = 22); and 6.8 +/- 0.6 fmol/mL for patients with gastritis (n = 28). These values are all higher than that of normal subjects (4.4 +/- 0.5 fmol/mL, n = 20; P < 0.001, P < 0.01, and P < 0.05, respectively). No significant differences in salivary IR-ET were noted between patients with a normal esophagogastroduodenoscopy and patients with esophagitis (3.8 +/- 0.7 fmol/mL, n = 4) or gastric cancer (5.3 +/- 1.4 fmol/mL, n = 4). There were no significant differences in the salivary IR-ET levels between males and females. However, the salivary IR-ET levels in the smokers (8.0 +/- 0.6 fmol/mL, n = 38) were significantly higher (P < 0.01) than those of the non-smokers (6.0 +/- 0.4 fmol/mL, n = 58). There was no correlation of IR-ET levels with age. Our findings suggest that salivary endothelin may have a contributing role in certain gastroduodenal diseases.