介绍一种快速瑞氏-姬姆萨染色法

传统的瑞氏染色法染色时间长、细胞着色差,而姬姆萨染色法虽然细胞核着色好,但胞浆染色欠佳,本文介绍一种瑞氏姬姆萨染色液,使染色在30″内一步完成,染色效果和原法一致,此液尤其适用于临床常规工作。 1 染液的配制 1.1 贮存液:瑞氏染粉2.0g,姬姆萨染液0.6g,天青Ⅱ0.6g,甘油10ml,聚乙烯呲咯烷酮2.0g,甲醇1000ml混合即可。 1.2 磷酸盐缓冲液(pH6.2～6.8):磷酸二氢钾6.64g,磷酸氢工钠0.25g,白碳酸4ml,蒸馏水加至100ml。 1.3 染色应用液:将上述贮存液,缓冲液按3:1比例混     

混合染色法在骨髓细胞学检验中的应用

目的 用混合染色法对骨髓液涂片进行染色。方法 瑞氏染液与姬氏染液分别配制,再进行混合染色,并按“低浓度,长时间”的原则染色。结果 细胞浆(碱性蛋白)着色天蓝,胞浆颗粒染色鲜艳,细胞核(核酸)着色层次分明,对细胞的特殊结构如核仁、核膜显示清晰。结论 混合染色法适宜作骨髓液涂片细胞学检查,或血液涂片中幼稚细胞的鉴定。     

急性白血病不同病期巨核细胞数量及形态学动态观察

目的 :探讨巨核细胞形态及数量在急性白血病 (AL )治疗过程中的临床价值。方法 :采用骨髓常规涂片 ,瑞氏—姬姆萨混合染液染色 ,标准化计数的方法。结果 :6 1例 AL患者巨细胞在其治疗过程中可见动态变化。结论 :巨核细胞数量及形态变化有助于判断 AL 患者的预后及疗效的观察。     

两种尿沉渣检测法诊断肾小球性血尿特异性与敏感性比较

为了识别尿沉渣中的有形成分形态,临床上已有不少的染色方法,大多为活体染色,对干片染色的研究较少.活体染色的缺点是不能在油镜下观察,而干片染色通过加予尿沉渣等量的健康人血清,可使细胞的形态保持完整,清晰可辨,尤其是红细胞的形态变化在油镜下更好辨认.刘氏快速染液A、B 液在临床上应用非常广泛,主要对全血细胞染色进行形态分类,优点是操作快速,效果优于瑞氏染液,清晰可辨.     

骨髓穿刺涂片联合骨髓活检切片在骨髓增生异常综合征诊断中的价值

为探讨骨髓穿刺涂片与骨髓活检切片同步观察对诊断骨髓增生异常综合征(MDS)的临床意义,回顾性分析532例MDS,采用骨髓抽吸-活检切片双标本一步法取材,骨髓涂片行常规瑞氏染色及免疫酶标染色,骨髓活检行苏木精-吉姆萨-酸性品红染色及Gomori网状纤维染色,观察骨髓增生程度及三系病态造血。结果表明,骨髓活检对骨髓增生程度的判断优于骨髓穿刺涂片。粒系、巨核系病态造血的检出率,骨髓活检高于骨髓穿刺;红系病态造血的检出率,骨髓涂片高于骨髓活检;免疫酶标染色法对微小巨核细胞的检出率明显高于瑞氏染色法。结论:骨髓活检切片与骨髓穿刺涂片同步分析及联合应用免疫酶标染色法检测微小巨核细胞,明显提高MDS诊断的准确性。     

介绍一种血涂片快速染液

血涂片的快速染色方法很多,我们摸索用瑞氏液、美兰、天青Ⅰ号配方,经临床试用,得到满意效果,适用于门诊和急诊检验,现简介如下: 试剂配制:2％瑞氏液300毫升,蒸馏水195毫升,混匀,1％天青Ⅰ号液5毫升,美兰0.2克,充分混匀后,置室温备用。操作方法:取血片待干,滴加1～2滴染液于血膜体尾交界处,涂成薄膜,立即以高倍镜作白细胞分类,需进一步细胞辨认时,可冲去染液,干后油镜检查。     

介绍一种改良精液染色法

精液的形态学检查无论在病理、生理上都有重要意义，是男性生殖系统疾病诊断和鉴别的基本方法．涂片染色效果的优劣直接影响检验医师的辨认、诊断．瑞氏染色是我们临床一直采用的染色方法，但使用过程中染色液配后需较长时间放置方可使用，而且染色时间较长．我们在瑞氏染色液中加人表面活性剂聚乙二醇辛基本基醚（OP）后染色，并与原染液进行对比，效果极佳，现报告如下．1材料和方法1．1染色液配制瑞氏染粉1．0g，姬姆萨染粉1．0g，天青Ⅱ0，05g，甘油10ml，聚乙二醇辛基本基酸0．1ml，甲醇500ml，充分摇匀溶解．1．2染色方法取液化精液…     

骨髓增生异常综合征细胞形态学观察

目的：观察骨髓增生异常综合征(MDS)骨髓细胞形态学病态改变。方法：用瑞氏-姬姆萨混合染液染色，进行骨髓及外周血细胞学分析。结果：粒、红、巨核三系都有不同程度的病态造血。粒系主要以细胞核浆发育不平衡，内外浆、中性粒细胞核分叶不能或Pelger-Huet样畸形。结论：MDS三系均有不同程度的病态改变，患者以中老年居多，分型主要以RA为主。     

Auer小体在AML诊断中的价值

目的 :探讨 Auer小体在急性髓细胞白血病 (AML )诊断治疗中的价值。方法 :普通骨髓涂片进行瑞氏 -姬姆萨混合染色 ,观察有无 Auer小体。结果 :2 6 3例 AML患者中 ,有 136例含 Auer小体 ,其中 AML - M2 及 AML - M3患者的 Auer小体检出率明显高于 AML的其他亚型。结论 :Auer小体在 AML中有高度特异性。     

猴疟血片染色方法的改进

猴疟血片染色按常规是用5％姬氏染液染30分钟,染液需用量大,时间长。优选方法;分别对染液浓度、染色时间两个因素以对分法和分数法进行优选试验。优选结果:用2.5％浓度的姬氏染液,薄血片染色20分钟,厚血片染色26分钟时着色     

盐酸二甲双胍缓释胶囊处方筛选及工艺研究

目的：研究盐酸二甲双胍缓释胶囊的处方筛选及工艺。方法：以释放度作为判断标准,对包衣材料的种类用量及工艺进行考察,进行处方筛选。结果：素丸以微晶纤维素为空白丸心,聚维酮为粘合剂,采用溶液法上药；包衣液以Eudragit RS100为主料,邻苯二甲酸二乙酯为增塑剂,乙醇为溶剂,利用流化床技术制备缓释胶囊。结论：通过这种方法制得的盐酸二甲双胍缓释胶囊具有较好的缓释效果,有继续开发的价值。     

盐酸二甲双胍缓释片处方筛选及工艺研究

对盐酸二甲双胍缓释片的处方及工艺进行筛选和研究。方法：根据释放度作为判断原则，对缓释骨架材料、填充剂、粘合剂、润滑剂及包衣材料的种类、规格、用量及工艺等进行了考察，并在此基础上采用正交试验1．9(3^4)方案对处方进行筛选。结果：以HPMC K100M及HPMC K15M作为盐酸二甲双胍缓释片的基本骨架材料，微晶纤维素作为填充剂，硬脂酸镁为润滑剂，4％HPMCE5的70％乙醇溶液适量作为粘合剂制成片芯，采用薄膜包衣法，以欧巴代的70％乙醇溶液作为薄膜包衣材料，制得盐酸二甲双胍缓释骨架片。结论：本制剂工艺简单，所用各种辅料均为国产化，成本低，制得盐酸二甲双胍缓释片释放度符合规定。     

18例骨髓坏死的结果分析

目的:观察急、慢性白血病及恶性组织细胞病等血液病患者的骨髓坏死情况。方法:瑞-姬氏染色,油镜观察。结果:阳性检出率为6.4%,其中急性白血病的检出率最高为7.4%,与恶性组织细胞病及骨髓纤维化症等比较差异较明显(P<0.05),而与慢性白血病、骨髓转移癌及恶性淋巴瘤等比较则差异不显著(P>0.05)。结论:骨髓坏死中,Ⅲ级仅占16.7%,大部分为Ⅰ、Ⅱ级。     

Natural dyeing of cotton fabric with extracts from mango tree: A step towards sustainable dyeing

Recently, there has been a great deal of interest to circumvent the environmental problems associated with manufactured colouring agents by using natural dyes. This study focuses on extracting natural dyes from mango leave and mango peel and finds sustainable ways of cotton dyeing. Natural colouring agents were extracted using an aqueous extraction technique and the cotton fabric was dyed using the extracts followed by mordanting using different mordant types. The dyeing performance of extracted colouring agents were assessed in terms of colour values, dye absorption (%), colour fastness properties and colour strength. The mordanting mechanisms of the extracted dyes were also proposed. Dyes extracted from mango leaves results in more colourised material than dye drived from mango fruit extract. Moreover, the dye extract exhibited a deeper shade. Result also shows that cotton fabric dyed with the same dye extract but with different type of mordants resulted in a fabric that showed diverse colours. Moderate to good fastness values were recorded (4–5). Considering the dyes exhibited excellent colour fastness, it can be concluded that the natural dyes extracted from various parts of the mango plant could be an effective colouring agent for use on cotton fabrics.     

急性混合细胞白血病临床特征分析

目的探讨急性混合细胞白血病的临床特征及诊治。方法根据骨髓细胞形态学、细胞化学染色和免疫表型结果诊断急性混合细胞白血病,采用DVCP方案、DVLP方案或DOAP方案化疗并根据骨髓缓解的情况评价疗效。结果11例患者临床表现为头晕乏力伴低热、胸骨压痛、淋巴结肿大、脾大、肝大、皮肤浸润等,所有患者骨髓增生明显活跃,近半数患者属高白细胞白血病。10例患者进行了免疫表型分析,其中B-Ly+/My+双表型8例、T-Ly+/My+双表型和B+T-Ly+/My+型各1例。采用DVCP方案、DVLP方案化疗7例,CR率42.9%,4例采用DOAP方案化疗,CR率50.0%。结论急性混合细胞白血病以B淋巴细胞系和髓系两系抗原共同表达者最多,临床分型诊断复杂,治疗缓解率低,预后差。     

女金丸中合成染料的检测及其染色部位分析

目的：对女金丸中的合成染料进行检测,分析其染色部位,并推断可能的染色原因。方法：采用液相色谱和质谱联用法,对女金丸样品中的合成染料进行测定,并采用液相色谱分别对丸剂外层和内层的合成染料进行测定,以判断其染色部位。结果：样品中可检出苋菜红、亮蓝和日落黄3种合成染料;上述染料主要集中于丸剂外层,丸剂内层未检出或仅可检出微量上述染料。结论：在制剂过程中使用了苋菜红、亮蓝和日落黄对丸剂外层进行染色,以改善制剂外观性状,应规范制剂工艺流程。     

骨髓活检在急性非淋巴细胞白血病化疗前、后的应用

应用骨髓活检对15例初治ANLL化疗前、后骨髓病理变化进行定量分析。结果表明:骨髓活检标本对确定骨髓内细胞分布、间质变化、骨髓细胞增生程度和判断化疗效果均较骨髓穿刺涂片优越,二者相互配合可以比较全面地反映骨髓的病理变化。     

Microwave-assisted eco-dyeing of bio mordanted silk fabric using cinnamon bark (Cinnamomum Verum) based yellow natural dye

The revival of sustainable plant-based dyes, now a day is becoming the demands of the global community in every field. The current study has been concerned with the exploration of cinnamon bark as a source of yellow natural colorant for silk dyeing. The yellow dye has been extracted in various media and exposed to microwave treatment for up to 6 min. The results show that dyeing of irradiated fabric at 35 °C for 45 min using an irradiated extract of 3 pH containing 1g of salt as a leveling agent has given excellent results. Salt of Al& Fe as sustainable chemical mordants & extract of acacia, henna, rose, pomegranate and turmeric as sustainable bio-mordants employed have not only given new shades but also improved fastness ratings. Conclusively, the microwave treatment has not only enhanced the extraction yield of dye from cinnamon bark but also has made the process more sustainable and ecofriendly by utilizing bio-mordants extracts.     

气相置换法加工甘草饮片的工艺研究

目的:研究中药材水蒸气软化的机理进行水蒸气软化。方法:采用气相置换润药机软化甘草与普通方法进行比较;结果:采用气相置换润药机软化药材相比具有药材含水率低软化效果好、软化速度快、避免有效成份流失等特点。结论:药材可在低含水量的情况下,快速、均匀软化。     

Bio colourants from the waste leaves of Ginkgo biloba L. tree: Wool dyeing and antimicrobial functionalization against some antibiotic-resistant bacterial strains

This research focuses on flavonoid-based natural dyes for the coloration and antibacterial finishing of wool fabrics. In the present study, we accessed the suitability of biocolorants from Gingko biloba L. waste/fallen leaves extract to develop colored antibacterial wool fabrics to reduce the environmental pollution and dependency of the textile industry on the synthetic antibacterial agents. Specifically, we present a novel method to counteract the growing antibiotic resistance using G. biloba L. leaves extract as a potent antibacterial agent in conjunction with ampicillin. Natural colorants were extracted in 50% aqueous ethanol at 70 °C for 60 min under acid conditions of pH 3. Before dyeing procedures, the extracted dye powder was assessed for thermal stability by TGA analysis. Structural characterization of the extracted dye was done by UV–visible and FT-IR spectral analysis. Optimal dyeing results were achieved at 90 °C for 70 min with a dye concentration of 50% (o.w.f.). Additionally, dye adsorption (Isotherm constants) and dye performances were effectively reported during this study. Pseudo-second order and sips isotherm model provided the best fitting of experimental data with high regression coefficient (R² = 0.99). Valuated indicators (color strength (K/S), color characteristics, and fastness properties) of dyed wool fabric were effectively reported in response to washing pretreated with different metallic and natural mordants. The antibacterial potential of extracted natural dye was enhanced in combination with ampicillin (synergism) which can be correlated with the significant reduction in the corresponding MIC values. Fe²⁺ and Sn²⁺ salts demonstrated negative effects on the antibacterial performance of the dyed wool fabric, whereas most of the biomordants showed enhanced antibacterial performances. The effective use of the extracted yellow dye from G. biloba L. fallen/waste leaves in textile dyeing and finishing will enhance the industrial applications of the green waste over its normal applications.