350例病毒性肝炎患者检测HBV-DNA与HBV血清学标志物相关性研究

采用ＰＣＲ技术对３５０例各型病毒性肝炎进行检测，旨在研究血清ＨＢＶ－ＤＮＡ与血清ＨＢＶ－Ｍ之间的相关性，以确定ＰＣＲ法检测ＨＢＶ－ＤＮＡ的临床意义。并以ＨＢＶ－ＤＮＡ为依据进一步考察ＨＢＶ－Ｍ的临床价值。结果显示：在ＨＢｓＡｇ（＋）、ＨＢｅＡｇ（＋）、抗－ＨＢｃ（＋）病例中ＨＢＶ－ＤＮＡ（＋）者１０２例（９０．２７％），说明ＨＢｅＡｇ基本可反应病毒处于复制状态；在１３７例ＨＢｅＡｇ（－）／抗－ＨＢｅ（＋）中，ＨＢＶ－ＤＮＡ（＋）为６８例（４９．６３％），表明病毒活动并未终止；在抗－ＨＢｓ（＋）１４例中，ＨＢＶ－ＤＮＡ（＋）２例（１４．２９％），说明抗－ＨＢｓ出现后仍然可有ＨＢＶ复制；在ＨＢＶ－Ｍ均阴性的８５例中，ＨＢＶ－ＤＮＡ（＋）２３例（２７．０６％），说明不能单纯依靠ＨＢＶ－Ｍ检测来诊断乙肝，需定期查ＨＢＶ－ＤＮＡ或肝活体组织检查ＨＢＶ－ＤＮＡ、ＨＢｓＡｇ及ＨＢｃＡｇ。     

PCR法检测HBVDNA及其与乙肝病毒标志物关系探讨

采用聚合酶链反应（ＰＣＲ）法检测２５６例乙型肝炎患者血清中乙肝病毒ＤＮＡ （ＨＢ－ＶＤＮＡ），同时用ＥＬＩＳＡ法检测乙肝病毒标志物，即ＨＢｓＡｇ，抗－ＨＢｓ，ＨＢｅＡｇ，抗－ＨＢｅ，结果表明ＨＢＶＤＮＡ的检出率与肝病临床类型无明显关系，而与乙肝病毒标志物的存在状态有关。乙肝五项病毒标志物不同组合状态，血清ＨＢＶＤＮＡ检出率不同，以ＨＢｓＡｇ（＋），抗－ＨＢｃ（＋），ＨＢｅＡｇ（＋）的组合形式检出率     

乙肝患者血清HBV—DNA与e,c系统相关分析及临床意义

对３７４乙型肝患者血清用地高辛探针法检测ＨＢＶ－ＤＮＡ；用酶联法检测ＨＢｅＡｇ、抗ＨＢｅ、ＨＢｃＡｇ、抗ＨＢｃ－ＩｇＭ。结果：ＨＢＶ－ＤＮＡ与ＨＢｅＡｇ、抗ＨＢｅＡｇ、抗ＨＢｅ无明显相关（Ｐ〉０．０５），与ＨＢｃＡｇ、抗ＨＢＣ－ＩｇＭ呈正相关关系（ｒ＝０．１８－０．２，Ｐ〈０．０１）；ＨＢｅＡｇ组与抗ＨＢｅ组ＨＢＶ－ＤＮＡ检出率无明显差异（Ｐ〉０．０５），以ＨＢＶ－ＤＮＡ为标准评价ＨＢｅＡｇ、ＨＢ     

350例病毒性肝炎患者检测HBV—DNA与HBV血清学标志物相…

采用ＰＣＲ技术对３５０例各型病毒性肝炎进行检测，旨在研究血清ＨＢＶ－ＤＮＡ与血清ＨＢＶ－Ｍ之间的相关性，以确定ＰＣＲ法检测ＨＢＶ－ＤＮＡ的临床意义。并以ＨＢＶ－ＤＮＡ为依据进一步考察ＨＢＶ－Ｍ的临床价值。结果显示：在ＨＢｓＡｇ（＋）、ＨＢｅＡｇ（＋）、抗－ＨＢｃ（＋）病例中ＨＢＶ－ＤＮＡ（＋）者１０２例（９０．２７％），说明ＨＢｅＡｇ基本可反应病毒处于复制状态；因１３７例ＨＢｅＡｇ（－）／抗－ＨＢ     

应用聚合酶链反应检测HBV—DNA的临床价值

采用聚合酶链反应（ＰＣＲ）和ＥＬＩＳＡ法对５３０例乙肝患者（含１１８例病毒携带者）血清中ＨＢＶ－ＤＮＡ和乙肝病毒标志物进行检测，ＨＢＶ－ＤＮＡ检出率分别为１）ＨＢｓＡｇ“＋”、ＨＢｅＡｇ“＋”和抗－ＨＢｃ“＋”组为９６．８５％；２）ＨＢｓＡｇ“＋”、抗－ＨＢｅ“＋”和抗－ＨＢｃ“＋”组为４４．１２％；３）ＨＢｓＡｇ“＋”和抗－ＨＢｃ“＋”组为３５．８９％；４）ＨＢＶ标志物均阴性为１３．１１％；５）     

乙型肝炎血清学指标阳性母亲母乳HBV—DNA检测

随机取５０例乙肝血清学指标阳性母亲产后２－３ｄ人的初乳，采用聚合酶链反应（ＰＣＲ）技术检测初乳中ＨＢＶ－ＤＮＡ。结果：抗原阳性组乙肝病毒（ＨＢＶ－ＤＮＡ）的检出率为５０％，抗体阳性组ＨＢＶ－ＤＮＡ检出率为７．７％。抗原阳性组中，ＨＢｓＡｇ，ＨＢｅＡｇ及抗－ＨＢｃ３项阳性乾和ＨＢｓＡｇ，ＨＢｅＡｇ２基阳性者乳汁中ＨＢＶ－－ＤＮＡ检出率为１００％，说明该类乳汁具有极强传染性，抗体阳性组中单纯抗－ＨＢｓ     

应用PCR技术及ELISA法检测HBV的结果分析

本文报道用酶联免疫吸附法（ＥＬＩＳＡ）及聚合酶链反应（ＰＣＲ）技术检测６４６例似肝火患者血清乙肝病毒标志物（ＨＢＶ－Ｍ）－乙肝二对半及乙肝病毒ＤＮＡ（ＨＢＶ－ＤＮＡ）的分析结果。用ＥＬＩＳＡ法检出ＨＢｓＡｇ（＋），ＨＢｅＡｇ（＋）及ＨＢｅＡｇ（＋）ＨＢｃＡｂ（＋）这两种模式ＨＢＶ－ＤＮＡ（＋）检出率分别高达９５．６％（４４／４６）及９１．１％（１２３／１３５），在虽然有ＨＢｓＡｇ（＋）出现，无（或     

600例乙肝病毒标志物阳性血清中检出抗－HBcIgM结果分析

采用立可读ＥＬＩＳＡ法对６００例乙肝异常标志物患者血清同时做抗－ＨＢｃＩｇＭ标志物检测，结果显示抗－ＨＢｃＩｇＭ阳性检出率与乙肝标志物中的ＨＢｓＡｇ和抗－ＨＢｃ阳性，ＨＢｓＡｇ、ＨＢｅＡｇ和抗－ＨＢｃ阳性，ＨＢｓＡｇ、抗－ＨＢｃ和抗－ＨＢｃ阳性，单项抗－ＨＢｃ阳性，四种常见模式有密切关系，其检出率分别为１３．３％、１１．７６％。１０．Ｏ％、６．６％；而其它常见模式均未检出抗－ＨＢｃＩｇＭ阳性。提示：抗－ＨＢｃＩｇＭ只能在急性和早期感染乙型病毒性肝炎中检出。     

慢性重型乙型肝炎血清抗HBe阳性意义再评价

对４２例慢性重型乙型肝炎的血清ｅ系统作回顾性分析，所有病例ＨＢＶ－ＤＮＡ均阳性，ＨＢｅＡｇ（＋）／抗ＨＢｅ（－）组（３０例）和ＨＢｅＡｇ（－）／抗ＨＢｅ（＋）组（１２例），并发症发生率和病死率均无显著性差异（Ｐ＞０．０５）；前组存活３例（１０．０％），均未发生ＨＢｅＡｇ／抗ＨＢｅ血清转换。     

HBVe系统衍变的临床意义——附465例临床检验结果分析

对３９３例慢性乙肝，７２例乙肝病毒携带者进行乙肝两对半和ＨＢＶ－ＤＮＡ检测，结果为①ＨＢｅＡｇ（＋），ＨＢＶ－ＤＮＡ检出率８５．８％；②ＨＢｅＡｂ（＋），ＨＢＶ－ＤＮＡ检出率２５．９％；③ｅ系统均（－），ＨＢＶ－ＤＮＡ检出率２９．０％。①与②、①与③比较有极显著性差异（Ｐ＜０．０１），②与③比较无显著性差异（Ｐ＞０．０５）。说明ＨＢｅＡｇ和ＨＢＶ－ＤＮＡ密切相关，都是ＨＢＶ复制活跃，具有传染性的指标。部分ＨＢｅＡｇ（－）／ＨＢｅＡｂ（＋）、ＨＢＶ－ＤＮＡ（＋）可能与乙肝病毒基因变异有关     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β,sIL-2R,IL-6,TNF-α,AND IFN-v IN ACUTE CORONARY SYNDROME

Objectives To explore serum cytokines levels (including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS.Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v were measured by enzyme linked immunosorbent assay.Results Serum IL- 1 β, sIL-2R, IL-6, TNF-α were significantly higher in AMI group or UAP group compared to the control group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-v shows no significant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner's QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤ 6 scores group and ＞ 6 scores group.Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a useful marker reflecting disease stability.     

Possible Spread of adenovirus type 3 from poultry to humans ：indirect evidence from an outbreak in China

Objective:To explore the epidemiology and etiology for an outbreak of acute respiratory tract infection that occurred in one county of Jiangsu Province, China 2004. Methods: Only cases meeting the case definition were included in the study. We reviewed the medical records of the cases who were admitted to the local hospitals, interviewed cases by a standard questionnaire, and then described the epidemiotogic features and analyzed risk factors by means of a case-control study. We collected pharyngeal swab specimens and sent them to different laboratories for isolation and culture. The laboratory used different detection methods such as DIP, PCR, electron microscope examination and microneutralization assay, to identify and then type the positive specimens. Results:A total of 871 cases were reported during the period from April 18 to July 4,2004. The distribution of onset times presented two peaks, one in late May and another in middle June. The epidemic occurred mainly in the elementary and junior high schools in ten townships of one county, and the mean age of the cases was 12 years (range 7 months to 18 years). The course of the disease was acute, and was characterized by fever accompanied with sore throat and tonsillitis. The WBC count of cases was normal or elevated. The mean duration of illness was 5 days (range 2 to 12 days). No fatalities from illness were reported. A case-control study indicated that the possible risk factors were close contact with a case and/or poultry before onset and sharing of towels among members of the family. The typical CPE was observed through inoculating pharyngeal swab specimens into the HEP-2 cell cultures in different laboratories. An infection of adenovirus type 3 was verified by detecting positive specimens in different methods. Conclusion:This investigation demonstrated that the acute respiratory infection in cases was caused by adenovirus type 3. Cases occurred in over 70 schools in ten townships in 2004, and the route of transmission was possibly close contact with cases or droplet transmission.