中药石榴皮鞣质成分抗生殖器疱疹病毒作用

采用细胞培养技术,证实鞣质是石榴皮抗生殖器疱疹病毒的有效成分。其特点不仅能够抑制病毒在细胞内的增殖,更为重要的是显示较强的直接杀灭病毒和阻止其吸附细胞的作用。     

石榴皮鞣质对腺嘌呤性慢性肾衰大鼠保护作用的研究

 目的 观察石榴皮鞣质对腺嘌呤性慢性肾功能衰竭（ CRF ）大鼠的影响。 方法 用 250 mg·kg^-1 腺嘌呤溶液连续灌胃 21 d 使 Wistar 大鼠产生类似慢性肾衰的症状。 21 d 后,治疗组大鼠每天用石榴皮鞣质（ 40 , 20 , 10 mg·kg ^-1 ·d ^-1 ）灌胃,阳性组用 20% 尿毒清混悬液按 2.1 g ·kg ^-1 ·d ^-1 灌胃。 60 d 后收集尿液,处死大鼠,取血、留取肾组织,测定血 Ca ²⁺ 、 P ³⁺ 及血肌酐（ Scr ）、尿素氮（ BUN ）、血脂和 24 h 尿蛋白定量、尿 Ca ²⁺ 、 P ³⁺ ,进行统计学处理,并进行形态学观察。 结果 石榴皮鞣质对慢性肾衰竭大鼠的血 Ca ²⁺ 、 P ³⁺ 及 Scr 、 BUN 和 24 h 尿蛋白定量、尿 Ca ²⁺ 、 P ³⁺ 有不同程度的改善。 结论 石榴皮鞣质对腺嘌呤导致的大鼠慢性肾衰竭有一定的保护作用。     

石榴皮中鞣质和黄酮类化合物抑菌作用的实验研究

目的探讨石榴皮中鞣质和黄酮类化合物的体外抑菌活性效果并确认其有效部位。方法提取两种化合物,采用试管两倍稀释法对金黄色葡萄球菌、福氏痢疾杆菌、沙门氏菌、大肠杆菌、绿脓杆菌和白色念珠菌进行体外抑菌实验。结果石榴皮中鞣质类化合物对金黄色葡萄球菌、福氏痢疾杆菌、沙门氏菌、大肠杆菌、绿脓杆菌和白色念珠菌均有不同程度的抑菌作用,而黄酮类化合物对绿脓杆菌和白色念珠菌抑菌效果不强。结论石榴皮中鞣质和黄酮类化合物有一定的抑菌作用,且鞣质类化合物呈现广谱抗菌特性,并具有抗耐药菌作用。     

石榴皮中鞣质各组分对淋球菌敏感性的研究

淋病是流行最广泛的一种性传播疾病,虽然近年来各类抗生素不断问世,但由于抗生素的滥用和临床上不规范的治疗,使淋球菌对抗生素的敏感性迅速发生改变,且合并症病人日益增多,使疾病更具有隐匿性与传染性,给临床诊断和治疗带来新的问题。有文献报道,石榴皮的提取物有较强的抑制淋     

石榴皮鞣质改善大鼠肾纤维化作用研究

目的 研究石榴皮鞣质对肾纤维化大鼠肾功能的改善作用。方法 将SD大鼠随机分为5组,假手术组,模型组,缬沙坦组,石榴皮鞣质高剂量和低剂量（60、20 mg/kg,按鞣质计）组。除了假手术组,其余各组采用左侧输尿管结扎方法制备肾纤维化模型,假手术组的输尿管不结扎和剪断。术后当天开始ig给药,分别于术后1、2、3周检测各组大鼠尿液中的24 h尿蛋白、Ca²⁺和P³⁺浓度,检测血清中的血肌酐（Scr）和尿素氮（BUN）,并进行肾组织HE、Masson染色,从形态学角度考察石榴皮鞣质对肾纤维化大鼠各项指标的影响。结果 HE、Masson染色结果显示,模型组大鼠肾组织受到损伤,左侧肾脏肾纤维化,造模成功;生理指标检测结果显示,左侧输尿管结扎后大鼠尿液的24 h尿蛋白,血清Scr、BUN偏离正常水平,Ca²⁺浓度上调,P³⁺浓度下调。石榴皮鞣质干预后,各项指标均具有恢复正常水平的趋势,石榴皮鞣质对大鼠肾纤维化具有改善作用。结论 石榴皮鞣质具有改善大鼠肾纤维化的作用,并能够调节肾纤维化大鼠机体钙磷代谢的失常。     

石榴皮提取物对大鼠实验性结肠炎的保护作用

目的:研究石榴皮对大鼠实验性结肠炎组织损伤的保护作用及其机制。方法:用三硝基苯磺酸建立大鼠实验性结肠炎模型,灌胃停药2周后评价大鼠结肠粘膜损伤指数(CMDI),检测结肠组织髓过氧化物酶(MPO)、超氧化物歧化酶(SOD)谷胱甘肽过氧化物酶(GSH-PX)活性和丙二醛(MDA)含量。结果:石榴皮200、400、800mg/kg灌胃能不同程度降低结肠炎模型大鼠CMD I、MPO活性,减少MDA含量,提高SOD、GSH-PX活性。结论:石榴皮对TNBS所致大鼠实验性结肠炎有明显保护作用。     

正交设计优选石榴皮鞣质的提取工艺△

目的:优化石榴皮鞣质的提取工艺.方法:正交试验法考察丙酮冷浸、回流和超声提取对提取率的影响,用干酪素法测总鞣质含量.结果:不同的丙酮提取工艺中,总鞣质提取率高低顺序为回流法超声法冷浸法.结论:提取石榴皮鞣质的最佳条件为10倍量70%丙酮水浴回流提取2次,每次0.5h.     

肠道菌群对石榴皮鞣质的分解代谢作用研究

目的考察肠道菌群对石榴皮鞣质的分解代谢作用,阐明石榴皮鞣质在肠道的代谢途径。方法将石榴皮鞣质与大鼠肠道菌液共同孵育4、6、8、12、24、48 h后,检测不同时间点溶液中的代谢成分。结果通过LC-MS/MS从石榴皮鞣质肠道菌群孵育液中共鉴别出了10个代谢产物,分别为尿石素B、没食子酸、焦性没食子酸、3-O-甲基没食子酸、尿石素A、甲基化尿石素A、尿石素C、尿石素-M6、葡萄糖醛酸化尿石素A、葡萄糖醛酸化尿石素C。结论石榴皮鞣质在肠道菌群中主要通过代谢酶的水解、还原等作用将其代谢成具有生理活性的小分子化合物,这些化合物有可能是石榴皮鞣质在机体内发挥药理活性的物质基础。     

炮制对石榴皮中没食子酸、鞣花酸和鞣质含量的影响

目的:研究炮制对石榴皮中没食子酸、鞣花酸和鞣质含量的影响。方法:以没食子酸、鞣花酸和鞣质含量为指标,对石榴皮生品和炒炭品进行比较。用HPLC法测定没食子酸和鞣花酸含量,用UV法测定鞣质含量。结果:石榴皮经炒炭后没食子酸和鞣花酸含量较生品依次增加124.76%和122.22%,而鞣质含量较生品降低56.55%。结论:加热炮制对石榴皮中没食子酸、鞣花酸和鞣质含量有显著影响。研究结果为石榴皮饮片的炮制和质量控制提供实验依据。     

Quercetin and naringenin; effects on ulcer formation and gastric secretion in rats

The antiulcerogenic effect of two flavonoids, quercetin and naringenin, in acute gastric ulcer (cold-restraint and pylorus-ligated) has been investigated. In both models quercetin and naringenin presented a significant decrease in ulcer index with respect to the control group. Nevertheless, in the Shay-ligated method no decrease in either volume, acidity or pepsin were observed, however, a significant difference was found in histamine secretion values.     

Studies on the antiulcer activity of Moringa oleifera leaf extract on gastric ulcer models in rats

The methanol fraction of M. oleifera leaf extract was found to possess significant protective actions in acetylsalicylic acid, serotonin and indomethacin induced gastric lesions in experimental rats. A significant enhancement of the healing process in acetic acid—induced chronic gastric lesions was also observed with the extract-treated animals.     

The antiulcer activity of Garcinia cambogia extract against indomethacin-induced gastric ulcer in rats.

Garcinia cambogia extract is a herbal preparation that has been suggested as useful in the treatment of gastrointestinal disorders. In the present study this drug was tested for its antiulcerogenic effect. Oral pretreatment with Garcinia cambogia fruit extract (1 g/kg body wt/day) for 5, 10 or 15 days protected the gastric mucosa against the damage induced by indomethacin (20 mg/kg body wt). The volume and acidity of the gastric juice decreased in the pretreated rats. The glycoprotein levels of the gastric contents which were decreased in the untreated rats, maintained near normal levels in the pretreated rats. Protein which was elevated in the gastric juice of untreated rats, showed near normal levels in the pretreated rats. Garcinia cambogia was able to decrease the acidity and to increase the mucosal defence in the gastric areas, thereby justifying its use as an antiulcerogenic agent.     

Antiulcerogenic activity of the essential oil of Baccharis dracunculifolia on different experimental models in rats

Baccharis dracunculifolia DC (Asteraceae), a native plant from Brazil, commonly known as ‘Alecrimdo‐campo’ is widely used in folk medicine to treat inflammation, hepatic disorders and stomach ulcers, and it is the most important botanical source of Southeastern Brazilian propolis, known as green propolis. Its essential oil is composed of non‐oxygenated and oxygenated terpenes. In this work, the effects of the essential oil obtained from the aerial parts of B. dracunculifolia on gastric ulcers were evaluated. The antiulcer assays were undertaken using the following protocols in rats: nonsteroidal antiinflammatory drug (NSAID)‐induced ulcer, ethanol‐induced ulcer, stress‐induced ulcer, and determination of gastric secretion using ligated pylorus. The treatment in the doses of 50, 250 and 500 mg/kg of B. dracunculifolia essential oil significantly diminished the lesion index, the total lesion area and the percentage of lesions in comparison with both positive and negative control groups. With regard to the model of gastric secretion a reduction of gastric juice volume and total acidity was observed, as well as an increase in the gastric pH. No sign of toxicity was observed in the acute toxicity study. Considering the results, it is suggested that the essential oil of B. dracunculifolia could probably be a good therapeutic agent for the development of new phytotherapeutic medicine for the treatment of gastric ulcer. Copyright © 2009 John Wiley & Sons, Ltd.     

芦荟多糖对动物实验性胃溃疡的影响

目的研究芦荟多糖对动物实验性胃溃疡的影响。方法应用大鼠幽门结扎型胃溃疡模型、大鼠醋酸烧灼型胃溃疡模型、小鼠利血平致胃溃疡模型,观察芦荟多糖ig给药对实验性胃溃疡的预防与治疗作用及对大鼠胃液、胃酸、胃黏液、胃蛋白酶分泌及活性的影响。结果芦荟多糖对实验性胃溃疡具有明显的预防及治疗作用,并能抑制胃液分泌、胃酸分泌及减少胃蛋白酶、胃黏液的分泌,但对胃蛋白酶活性无明显影响。结论芦荟多糖对动物实验性胃溃疡具有良好的预防及治疗作用,并能抑制溃疡致病因子胃酸及胃蛋白酶的分泌。     

胃疡宁丸抗实验性胃溃疡的药效及机制研究

目的:研究胃疡宁丸抗实验性胃溃疡的药效及其机制.方法:采用大鼠醋酸注射法、水浸应激性、阿司匹林诱发及幽门结扎性胃溃疡模型,观察胃疡宁丸抗胃溃疡的作用并考察其对血清NO,EGF含量,胃黏膜血流量,PGE2含量,及对胃液分泌、胃酸含量、胃蛋白酶活性的影响.结果:胃疡宁丸能够降低各种胃溃疡模型的溃疡指数,升高NO,EGF含量,PGE2含量及胃黏膜血流量,抑制胃液分泌和胃酸含量及降低胃蛋白酶活性.结论:胃疡宁丸具有明显的抗实验性胃溃疡的作用,该作用与其减少胃黏膜损伤因子(胃酸、胃蛋白酶),增加胃黏膜屏障的功能,增强胃黏膜修复作用(NO,EGF,PGE2,胃黏膜血流量)有关.     

甘草酸铋抗实验性胃溃疡作用及机制研究

目的：研究甘草酸铋抗实验性胃溃疡的作用及其作用机制。方法：以SD大鼠和昆明种小鼠为研究对象,复制3种实验性胃溃疡动物模型(大鼠幽门结扎致胃溃疡模型、大鼠乙醇致胃溃疡模型和小鼠束缚水浸应激致胃溃疡模型)。将大鼠分成甘草酸铋700,350,175 mg·kg^-1剂量组、甲氰咪胍(200 mg·kg^-1)阳性对照组和0.1%EDTA空白对照组；小鼠分成甘草酸铋980,490,250 mg·kg^-1剂量组。测定胃黏膜损伤指数、胃液分泌量,胃液酸度,血清中一氧化氮(NO)含量,胃组织前列腺素(PGE₂)相对含量；结果：与模型对照组比较,甘草酸铋能使胃液量、胃酸浓度 、胃黏膜损伤指数减少(或降低)；血清NO含量和胃组织前列腺素含量增加。结论：甘草酸铋对实验性胃溃疡具有一定疗效,其作用机制可能与抑制胃酸分泌、改善胃黏膜微循环有关。     

Effects of naringenin and quercetin on experimental chronic gastric ulcer in rat. Studies on the histological findings

The effects of chronic intragastric administration of the flavonoid derivatives naringenin and quercetin (100 mg/kg body weight) on mucosal lesion induced by acetic acid were studied in the rat. The evolution of the healing process of the ulcer by histological measurement was also investigated. Ranitidine was used as a positive reference drug. Compounds were consecutively administered to different groups of rats for 8, 21 and 50 days. The values for the histological mucosa regeneration index as well as the morphological results, indicated that the naringenin cure potential in prolonged treatments was higher than that of the other drugs.     

复方丹蒲汤抗实验性胃溃疡的作用

目的:研究复方丹蒲汤(丹参、蒲公英、砂仁等)抗实验性胃溃疡的作用及其机制。方法:采用小鼠水浸应激性、利血平性、无水乙醇性、幽门结扎性胃溃疡模型观察复方丹蒲汤对胃溃疡的保护作用及对大鼠胃液分泌的影响;采用小鼠胃排空方法观察复方丹蒲汤对胃运动的影响;以热板法观察其镇痛作用。结果:复方丹蒲汤对小鼠水浸应激性、利血平性及无水乙醇性胃溃疡模型均有明显的保护作用;能抑制大鼠胃酸及胃蛋白酶的分泌,降低大鼠胃溃疡指数,减少溃疡面积;抑制小鼠胃排空;延长小鼠热刺激的痛反应时。结论:复方丹蒲汤具有抗实验性胃溃疡的作用。该作用可能与其抑制胃液分泌、胃平滑肌运动及镇痛作用有关。     

芩连合剂对实验性胃溃疡大鼠胃黏膜保护作用的机制研究

目的探讨芩连合剂(QLHJ)对急性胃溃疡大鼠胃黏膜的保护作用及其作用机制.方法采用无水乙醇灌胃法复制大鼠急性胃溃疡模型,测定胃黏膜细胞内丙二醛(MDA)、超氧化物歧化酶(SOD)的表达、谷胱苷肽过氧化物酶(GSH-Px)活力及钙离子浓度.结果模型组大鼠胃黏膜细胞内钙离子浓度较其他各组明显升高,与芩连合剂组和正常组比较有非常显著性差异(P＜0.01);SOD、GSH-Px的表达较其他各组明显降低,MDA表达较其他各组明显升高,与芩连合剂组、地尔硫组和正常组比较有显著性差异(P＜0.05,P＜0.01).结论QLHJ通过降低胃黏膜细胞内钙离子浓度的水平,降低胃黏膜细胞内MDA的表达和提高胃黏膜细胞内SOD和GSH-Px的表达,从而发挥其保护胃溃疡大鼠胃黏膜细胞的作用.