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人外周血林巴细胞松果体素受体鉴定及意义 总被引:9,自引:0,他引:9
为证明免疫组织上是否存在松果体素受体(MR)。以^125I-松果体系(M)为配体,应用放射配体结合法检测40例健康人外周血淋巴细胞^125I-M特异结合位点。结果:①^135I-M特异结合最大结合容量(Bmax)为0.36±0.10fmol/10^6细胞(217±60位点/细胞);平衡解离常数(Kd)为79±13pmol/L;②动力学分析显示K1=(1.24±0.31)×10^-7L·min^-1 相似文献
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大鼠感染性脑水肿时NMDA受体的变化 总被引:3,自引:0,他引:3
测定百日咳菌液(BP)所致大鼠感染性脑水肿(IBE)对3HMK801与大脑皮层神经细胞特异结合的影响。从大鼠左颈总动脉注入BP,制备IBE模型。雄性SD大鼠随机分3组:①对照组(NS,n=11);②IBE组(BP,n=12);③MK801+BP预处理组(MK801,n=4)。02ml/kg体重生理盐水或BP注入左颈总动脉作为NS或BP组;每天腹腔注射05mgMK801/kg体重,共2天,然后注入BP为MK801组。注射BP24小时后断头取脑。N甲基D天冬氨酸(NMDA)受体与3HMK801的特异结合用放射配体结合分析法测定,Scatchard作图。结果,NS及BP组的Bmax分别为0623±0082和0606±0087pmol/mg蛋白质(t=048,P>005),Kd分别为431±42和305±30μmol/L(t=78,P<005)。MK801预处理减少NMDA受体的特异结合。表明在此脑水肿模型中NMDA受体总数未发生变化,但亲和力明显增高,其增高能被预先腹腔注入的MK801抑制。 相似文献
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为探讨γ干扰素(IFN)对胃癌细胞(SGC7901)肿瘤坏死因子(TNF)受体的影响及与肿瘤坏死因子突变体(TNFm)协同抗肿瘤的机制,以125ITNFm为配体,用放射受体分析法研究γIFN对SGC7901细胞TNF受体表达及与TNFm内化和降解的影响。并用MTT法研究γIFN与TNFm协同抗肿瘤作用。结果:γIFN使SGC7901细胞TNF受体的数量由056×10-11nmol/细胞增至094×10-11nmol/细胞,而对受体的亲和力无影响(Kd为278×10-11和264×10-10mol/L,t=12,P>05);γIFN可明显增加TNFm内化和降解的绝对数量,而对其比率无影响。γIFN使TNFm对SGC7901细胞的最大杀伤率达到96%(t=52,P<001)。提示:γIFN通过上调TNF受体而加强TNFm的体外抗肿瘤作用。 相似文献
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125IBMIPP大鼠体内分布及代谢干预对其心肌分布的影响李京波黄钢李志善刘平安陈可靖附表125IBMIPP在大鼠组织中的分布(%ID/g)脏器注射时间(min)5153060心386±046337±039327±032302±0?.. 相似文献
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rIL—2的^125I标记及淋巴细胞IL—2受体的鉴定 总被引:1,自引:0,他引:1
为测定白细胞介素-2受体(IL-2R)的表达水平,应用lodogen法对重且IL2(rIL-2)进行^125I标记,以放射配基结合分析法对大鼠脾淋巴细胞表面IL-2R进行鉴定。结果表明。rIL-2蛋白质标记率为58.8%,比活度为488.4kBq/μg;^125IIL-2在10-150pmol/L范围内与淋巴细胞IL-2R呈特异性结合,为可饱和的双分子过程,最大结合容量和平衡解离常数分别为10.0 相似文献
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屏气相位对比MRI评价正常人冠状动脉血流储备 总被引:4,自引:1,他引:3
目的 研究屏气相位对比MR(PCMR) 技术测定正常人冠状动脉血流储备(CFR) 的可行性。方法 健康志愿者15 例(男12 例,女3 例)。采用快速屏气K空间分段PCMR技术,测定冠状动脉左前降支(LAD)静息状态和静注潘生丁负荷状态下的舒张期峰值血流速度、容积流率及CFR。结果基础状态LAD容积流率为(35-4±13-3) ml/min,静注潘生丁后明显增加至(155-4 ±66-8) ml/min(P<0-01), 平均CFR为4-4±0-73。基础状态LAD峰值流速为(22-5±3-9)cm /s, 静注潘生丁后明显增加至(63-8±16-7)cm/s( P< 0-01), 两者比值为2-9±0-72 。结论 该研究表明采用屏气PCMRI流速编码技术可以定量测定正常人冠状动脉容积流率、峰值流速,从而评价CFR。 相似文献
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目的建立双核素阴茎海绵体动态显像的定量分析方法,用于评价血管性阳萎。方法用99mTcRBC作阴茎海绵体动脉系统显像,131I邻碘马尿酸(OIH)作阴茎海绵体静脉系统显像,并在盐酸罂粟碱负荷下连续测阴茎海绵体内放射性变化。观察12例正常男子和42例阳萎病人阴茎海绵体血液动力学变化。结果12例正常男子99mTc阴茎动脉系统显像指数(TPIA)为207±084(正常值>060);131I阴茎静脉系统显像指数(IPIV)为-034±012(正常值>-048)。根据TPIA和IPIV的结果,42例阳萎病人被分为3组:13例TPIA、IPIV正常者为非血管性阳萎组,其TPIA为190±069,IPIV为-036±008;21例TPIA正常、IPIV异常者为静脉性阳萎组,TPIA为198±010,IPIV为-067±012;8例TPIA异常、IPIV正常或异常者,其TPIA为034±021,IPIV为-055±011。结论双核素阴茎海绵体动态显像对评价血管性阳萎,特别是鉴别静脉和动脉系统方面的病理状况是一种安全、无创、客观和有价值的方法 相似文献
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家兔注射^125I—标记重组人表皮生长因子后的药代动力学 总被引:2,自引:0,他引:2
用Iodogen法制备125IrhEGF,SDSPAGE鉴定放射纯度为(97.1±0.45)%。家兔静脉注射125IrhEGF后8h内血浆总放射性保持在高水平,反相高压液相色谱(RHPLC)测得125IrhEGF分量迅速下降,亲水性125I标记物分量增高。125IrhEGF浓度时间曲线符合二室开放模型,t1/2α和t1/2β分别为9min和11.6h。组织浓度梯度为尿>甲状腺>肠内容>肾>血浆>胆汁>颌下腺>肺。8h内尿排泄(20.3±5.1)%的注入放射性。 相似文献
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为探讨检测肺泡清除功能的方法以及肺泡清除功能变化的可能机理,应用99mTcDTPA雾化吸入2~3分钟后,启动γ相机连续动态采集30分钟,以前5分钟的曲线斜率为K1,5分钟后的曲线斜率为K2。结果:正常对照组的时间放射性曲线为单室模型曲线,Y=Ae-kt,在半对数坐标上为近似斜线,K1=(908±479)×10-3,K2=(735±325)×10-3(x±s);吸烟、甲亢和哮喘组的K1、K2均高于正常(t值3164~10230,P<001),为双室或三室以上的多室模型曲线。结果表明:上肺与中肺的K1有显著差异,K1是一个灵敏指标。各病变组呼吸膜改变的主要原因是肺泡上皮或毛细血管内皮受损所致。 相似文献
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电针治疗前后帕金森病模型大鼠纹状体D2受体与多巴胺含量的对比研究 总被引:3,自引:1,他引:2
目的研究经电针治疗前后偏侧帕金森病(PD)模型大鼠纹状体多巴胺(DA)D2受体的变化,并将其与DA及其代谢产物的含量进行对比分析。方法用125I3碘2羟基6甲氧基N[(1乙基2吡咯烷)甲基]苯酰胺(IBZM)脑D2受体放射自显影、高效液相电化学检测器(HPLCECD)检测治疗前组、电针治疗组及治疗空白对照组偏侧PD模型大鼠纹状体DA及其代谢产物高香草酸(HVA)、3,4二羟苯乙酸(DOPAC)含量。结果电针治疗组患侧纹状体DA、HVA、DOPAC含量较治疗前组及治疗空白对照组增高(P<005);电针治疗组患侧纹状体/小脑125IIBZM摄取比值为804±071,较健侧升高(2934±483)%,与治疗前组的(809±052)相比,差异无显著性(P>005),但低于治疗空白对照组的(861±063),此值较健侧升高(3863±371)%。结论电针治疗可以提高大鼠实验性偏侧PD模型纹状体DA含量,并可抑制早期实验性PD时D2受体的上调效应 相似文献
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Knee injury and Osteoarthritis Outcome Score (KOOS) - validation of a Swedish version 总被引:5,自引:0,他引:5
E. M. Roos H. P. Roos C. Ekdahl L. S. Lohmander 《Scandinavian journal of medicine & science in sports》1998,8(6):439-448
The Knee injury and Osteoarthritis Outcome Score (KOOS) is a self-administered instrument measuring outcome after knee injury at impairment, disability, and handicap level in five subscales. Reliability, validity, and responsiveness of a Swedish version was assessed in 142 patients who underwent arthroscopy because of injury to the menisci, anterior cruciate ligament, or cartilage of the knee. The clinimetric properties were found to be good and comparable to the American version of the KOOS. Comparison to the Short Form-36 and the Lysholm knee scoring scale revealed expected correlations and construct validity. Item by item, symptoms and functional limitations were compared between diagnostic groups. High responsiveness was found three months after arthroscopic partial meniscectomy for all subscales but Activities of Daily Living. 相似文献
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Objective To investigate endovascular treatment of traumatic direct carotid-cavernous fistulas (CCF) and their complications such as pseudoaneurysms. Methods: Over a five-year period, 22 patients with traumatic direct CCFs were treated endovascularly in our institution. Thirteen patients were treated once with the result of CCF occluded, 8 twice and 1 three times. Treatment modalities included balloon occlusion of the CCF, sacrifice of the ipsilateral internal carotid artery with detachable balloon, coll embolization of the cavernous sinus and secondary pseudoaneurysms, and covered-stem management of the pseudoaneurysms. Results All the direct CCFs were successfully managed endovascularly. Four patients developed a pseudoaneurysm after the occlusion of the CCF with an incidence of pseudoaneurysm formation of 18.2% (4/22). A total number of 8 patients experienced permanent occlusion of the ICA with a rate of ICA occlusion reaching 36.4% (8/22). Followed up through telephone consultation from 6 months to 5 years, all did well with no recurrence of CCF symptoms and signs. Conclusion Traumatic direct CCFs can be successfully managed with endovascular means. The pseudoaneurysms secondary to the occlusion of the CCFs can be occluded with stent-assisted coiling and implantation of covered stents. 相似文献
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Acute limping may be the result of multiple pathologies in children. The differential diagnosis varies based on the age of the child. Irrespective of age, the initial imaging work-up includes AP and frog leg radiographs of the pelvis and ultrasound; MRI may sometimes be helpful. In children less than 3 years, infections and trauma are most frequent. MRI is the imaging modality of choice when osteomyelitis is clinically suspected. Between the ages of 3 and 10 years, transient synovitis of the hip and Legg-Calvé-Perthes disease are main considerations but infection, inflammation and focal bony lesions are also considered. In children over 10 years, slipped capital femoral epiphysis also is considered. 相似文献
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Introduction Ankle sprains are the most common musculoskeletal injury that occurs inathletes particularly in sports that require jumping landing on one foot such assoccer and basketball 《中国运动医学杂志》2008,27(3):378-381
Introduction Ankle sprains are the most common musculo-skeletal injury that occurs in athletes,particularly in sports that require jumping and landing on one foot such as soccer,and basketball(1-4).These injuries often result in significant time loss from participation,long-term disability,and have a major impact on health care costs and resources(5-8). 相似文献
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Martin J.Gibala 《中国运动医学杂志》2008,27(3)
KEY POINTS ·High-intensity interval training(HIT)is characterized by repeated sessions of relatively brief,intermittent exercise.often performed with an“a11 out”effort or at an intensity close to that which elicits peak oxygen uptake(i.e.,≥90%of VO2 peak). 相似文献
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Tucker VC Hopwood AJ Sprecher CJ McLaren RS Rabbach DR Ensenberger MG Thompson JM Storts DR 《Forensic science international. Genetics》2011,5(5):436-448
In response to the ENFSI and EDNAP groups’ call for new STR multiplexes for Europe, Promega® developed a suite of four new DNA profiling kits. This paper describes the developmental validation study performed on the PowerPlex® ESI 16 (European Standard Investigator 16) and the PowerPlex® ESI 17 Systems. The PowerPlex® ESI 16 System combines the 11 loci compatible with the UK National DNA Database®, contained within the AmpFlSTR® SGM Plus® PCR Amplification Kit, with five additional loci: D2S441, D10S1248, D22S1045, D1S1656 and D12S391. The multiplex was designed to reduce the amplicon size of the loci found in the AmpFlSTR® SGM Plus® kit. This design facilitates increased robustness and amplification success for the loci used in the national DNA databases created in many countries, when analyzing degraded DNA samples. The PowerPlex® ESI 17 System amplifies the same loci as the PowerPlex® ESI 16 System, but with the addition of a primer pair for the SE33 locus. Tests were designed to address the developmental validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM), and those of the DNA Advisory Board (DAB). Samples processed include DNA mixtures, PCR reactions spiked with inhibitors, a sensitivity series, and 306 United Kingdom donor samples to determine concordance with data generated with the AmpFlSTR® SGM Plus® kit. Allele frequencies from 242 white Caucasian samples collected in the United Kingdom are also presented. The PowerPlex® ESI 16 and ESI 17 Systems are robust and sensitive tools, suitable for the analysis of forensic DNA samples. Full profiles were routinely observed with 62.5 pg of a fully heterozygous single source DNA template. This high level of sensitivity was found to impact on mixture analyses, where 54–86% of unique minor contributor alleles were routinely observed in a 1:19 mixture ratio. Improved sensitivity combined with the robustness afforded by smaller amplicons has substantially improved the quantity of data obtained from degraded samples, and the improved chemistry confers exceptional tolerance to high levels of laboratory prepared inhibitors. 相似文献