人外周血林巴细胞松果体素受体鉴定及意义

为证明免疫组织上是否存在松果体素受体（ＭＲ）。以＾１２５Ｉ－松果体系（Ｍ）为配体，应用放射配体结合法检测４０例健康人外周血淋巴细胞＾１２５Ｉ－Ｍ特异结合位点。结果：①＾１３５Ｉ－Ｍ特异结合最大结合容量（Ｂｍａｘ）为０．３６±０．１０ｆｍｏｌ／１０＾６细胞（２１７±６０位点／细胞）；平衡解离常数（Ｋｄ）为７９±１３ｐｍｏｌ／Ｌ；②动力学分析显示Ｋ１＝（１．２４±０．３１）×１０＾－７Ｌ·ｍｉｎ＾－１     

大鼠感染性脑水肿时NMDA受体的变化

测定百日咳菌液（ＢＰ）所致大鼠感染性脑水肿（ＩＢＥ）对３ＨＭＫ８０１与大脑皮层神经细胞特异结合的影响。从大鼠左颈总动脉注入ＢＰ，制备ＩＢＥ模型。雄性ＳＤ大鼠随机分３组：①对照组（ＮＳ，ｎ＝１１）；②ＩＢＥ组（ＢＰ，ｎ＝１２）；③ＭＫ８０１＋ＢＰ预处理组（ＭＫ８０１，ｎ＝４）。０２ｍｌ／ｋｇ体重生理盐水或ＢＰ注入左颈总动脉作为ＮＳ或ＢＰ组；每天腹腔注射０５ｍｇＭＫ８０１／ｋｇ体重，共２天，然后注入ＢＰ为ＭＫ８０１组。注射ＢＰ２４小时后断头取脑。Ｎ甲基Ｄ天冬氨酸（ＮＭＤＡ）受体与３ＨＭＫ８０１的特异结合用放射配体结合分析法测定，Ｓｃａｔｃｈａｒｄ作图。结果，ＮＳ及ＢＰ组的Ｂｍａｘ分别为０６２３±００８２和０６０６±００８７ｐｍｏｌ／ｍｇ蛋白质（ｔ＝０４８，Ｐ＞００５），Ｋｄ分别为４３１±４２和３０５±３０μｍｏｌ／Ｌ（ｔ＝７８，Ｐ＜００５）。ＭＫ８０１预处理减少ＮＭＤＡ受体的特异结合。表明在此脑水肿模型中ＮＭＤＡ受体总数未发生变化，但亲和力明显增高，其增高能被预先腹腔注入的ＭＫ８０１抑制。     

γ干扰素对胃癌细胞TNF受体表达和TNF突变体代谢的影响

为探讨γ干扰素（ＩＦＮ）对胃癌细胞（ＳＧＣ７９０１）肿瘤坏死因子（ＴＮＦ）受体的影响及与肿瘤坏死因子突变体（ＴＮＦｍ）协同抗肿瘤的机制，以１２５ＩＴＮＦｍ为配体，用放射受体分析法研究γＩＦＮ对ＳＧＣ７９０１细胞ＴＮＦ受体表达及与ＴＮＦｍ内化和降解的影响。并用ＭＴＴ法研究γＩＦＮ与ＴＮＦｍ协同抗肿瘤作用。结果：γＩＦＮ使ＳＧＣ７９０１细胞ＴＮＦ受体的数量由０５６×１０－１１ｎｍｏｌ／细胞增至０９４×１０－１１ｎｍｏｌ／细胞，而对受体的亲和力无影响（Ｋｄ为２７８×１０－１１和２６４×１０－１０ｍｏｌ／Ｌ，ｔ＝１２，Ｐ＞０５）；γＩＦＮ可明显增加ＴＮＦｍ内化和降解的绝对数量，而对其比率无影响。γＩＦＮ使ＴＮＦｍ对ＳＧＣ７９０１细胞的最大杀伤率达到９６％（ｔ＝５２，Ｐ＜００１）。提示：γＩＦＮ通过上调ＴＮＦ受体而加强ＴＮＦｍ的体外抗肿瘤作用。     

^125I—BMIPP大鼠体内分布及代谢干预对其心肌分布的影响

１２５ＩＢＭＩＰＰ大鼠体内分布及代谢干预对其心肌分布的影响李京波黄钢李志善刘平安陈可靖附表１２５ＩＢＭＩＰＰ在大鼠组织中的分布（％ＩＤ／ｇ）脏器注射时间（ｍｉｎ）５１５３０６０心３８６±０４６３３７±０３９３２７±０３２３０２±０?..     

rIL—2的^125I标记及淋巴细胞IL—2受体的鉴定

为测定白细胞介素－２受体（ＩＬ－２Ｒ）的表达水平，应用ｌｏｄｏｇｅｎ法对重且ＩＬ２（ｒＩＬ－２）进行＾１２５Ｉ标记，以放射配基结合分析法对大鼠脾淋巴细胞表面ＩＬ－２Ｒ进行鉴定。结果表明。ｒＩＬ－２蛋白质标记率为５８．８％，比活度为４８８．４ｋＢｑ／μｇ；＾１２５ＩＩＬ－２在１０－１５０ｐｍｏｌ／Ｌ范围内与淋巴细胞ＩＬ－２Ｒ呈特异性结合，为可饱和的双分子过程，最大结合容量和平衡解离常数分别为１０．０     

屏气相位对比MRI评价正常人冠状动脉血流储备

目的　研究屏气相位对比ＭＲ（ＰＣＭＲ） 技术测定正常人冠状动脉血流储备（ＣＦＲ） 的可行性。方法　健康志愿者１５ 例（男１２ 例,女３ 例）。采用快速屏气Ｋ空间分段ＰＣＭＲ技术,测定冠状动脉左前降支（ＬＡＤ）静息状态和静注潘生丁负荷状态下的舒张期峰值血流速度、容积流率及ＣＦＲ。结果基础状态ＬＡＤ容积流率为（３５－４±１３－３） ｍｌ／ｍｉｎ,静注潘生丁后明显增加至（１５５－４ ±６６－８） ｍｌ／ｍｉｎ（Ｐ＜０－０１）, 平均ＣＦＲ为４－４±０－７３。基础状态ＬＡＤ峰值流速为（２２－５±３－９）ｃｍ ／ｓ, 静注潘生丁后明显增加至（６３－８±１６－７）ｃｍ／ｓ（ Ｐ＜ ０－０１）, 两者比值为２－９±０－７２ 。结论　该研究表明采用屏气ＰＣＭＲＩ流速编码技术可以定量测定正常人冠状动脉容积流率、峰值流速,从而评价ＣＦＲ。     

双核素阴茎海绵体动态显像对血管性阳萎的评价

目的建立双核素阴茎海绵体动态显像的定量分析方法，用于评价血管性阳萎。方法用９９ｍＴｃＲＢＣ作阴茎海绵体动脉系统显像，１３１Ｉ邻碘马尿酸（ＯＩＨ）作阴茎海绵体静脉系统显像，并在盐酸罂粟碱负荷下连续测阴茎海绵体内放射性变化。观察１２例正常男子和４２例阳萎病人阴茎海绵体血液动力学变化。结果１２例正常男子９９ｍＴｃ阴茎动脉系统显像指数（ＴＰＩＡ）为２０７±０８４（正常值＞０６０）；１３１Ｉ阴茎静脉系统显像指数（ＩＰＩＶ）为－０３４±０１２（正常值＞－０４８）。根据ＴＰＩＡ和ＩＰＩＶ的结果，４２例阳萎病人被分为３组：１３例ＴＰＩＡ、ＩＰＩＶ正常者为非血管性阳萎组，其ＴＰＩＡ为１９０±０６９，ＩＰＩＶ为－０３６±００８；２１例ＴＰＩＡ正常、ＩＰＩＶ异常者为静脉性阳萎组，ＴＰＩＡ为１９８±０１０，ＩＰＩＶ为－０６７±０１２；８例ＴＰＩＡ异常、ＩＰＩＶ正常或异常者，其ＴＰＩＡ为０３４±０２１，ＩＰＩＶ为－０５５±０１１。结论双核素阴茎海绵体动态显像对评价血管性阳萎，特别是鉴别静脉和动脉系统方面的病理状况是一种安全、无创、客观和有价值的方法     

家兔注射^125I—标记重组人表皮生长因子后的药代动力学

用Ｉｏｄｏｇｅｎ法制备１２５ＩｒｈＥＧＦ，ＳＤＳＰＡＧＥ鉴定放射纯度为（９７．１±０．４５）％。家兔静脉注射１２５ＩｒｈＥＧＦ后８ｈ内血浆总放射性保持在高水平，反相高压液相色谱（ＲＨＰＬＣ）测得１２５ＩｒｈＥＧＦ分量迅速下降，亲水性１２５Ｉ标记物分量增高。１２５ＩｒｈＥＧＦ浓度时间曲线符合二室开放模型，ｔ１／２α和ｔ１／２β分别为９ｍｉｎ和１１．６ｈ。组织浓度梯度为尿＞甲状腺＞肠内容＞肾＞血浆＞胆汁＞颌下腺＞肺。８ｈ内尿排泄（２０．３±５．１）％的注入放射性。     

^99mTc—DTPA雾化吸入检测肺泡清除功能及初步分析

为探讨检测肺泡清除功能的方法以及肺泡清除功能变化的可能机理，应用９９ｍＴｃＤＴＰＡ雾化吸入２～３分钟后，启动γ相机连续动态采集３０分钟，以前５分钟的曲线斜率为Ｋ１，５分钟后的曲线斜率为Ｋ２。结果：正常对照组的时间放射性曲线为单室模型曲线，Ｙ＝Ａｅ－ｋｔ，在半对数坐标上为近似斜线，Ｋ１＝（９０８±４７９）×１０－３，Ｋ２＝（７３５±３２５）×１０－３（ｘ±ｓ）；吸烟、甲亢和哮喘组的Ｋ１、Ｋ２均高于正常（ｔ值３１６４～１０２３０，Ｐ＜００１），为双室或三室以上的多室模型曲线。结果表明：上肺与中肺的Ｋ１有显著差异，Ｋ１是一个灵敏指标。各病变组呼吸膜改变的主要原因是肺泡上皮或毛细血管内皮受损所致。     

电针治疗前后帕金森病模型大鼠纹状体D2受体与多巴胺含量的对比研究

目的研究经电针治疗前后偏侧帕金森病（ＰＤ）模型大鼠纹状体多巴胺（ＤＡ）Ｄ２受体的变化,并将其与ＤＡ及其代谢产物的含量进行对比分析。方法用１２５Ｉ３碘２羟基６甲氧基Ｎ［（１乙基２吡咯烷）甲基］苯酰胺（ＩＢＺＭ）脑Ｄ２受体放射自显影、高效液相电化学检测器（ＨＰＬＣＥＣＤ）检测治疗前组、电针治疗组及治疗空白对照组偏侧ＰＤ模型大鼠纹状体ＤＡ及其代谢产物高香草酸（ＨＶＡ）、３,４二羟苯乙酸（ＤＯＰＡＣ）含量。结果电针治疗组患侧纹状体ＤＡ、ＨＶＡ、ＤＯＰＡＣ含量较治疗前组及治疗空白对照组增高（Ｐ＜００５）;电针治疗组患侧纹状体／小脑１２５ＩＩＢＺＭ摄取比值为８０４±０７１,较健侧升高（２９３４±４８３）％,与治疗前组的（８０９±０５２）相比,差异无显著性（Ｐ＞００５）,但低于治疗空白对照组的（８６１±０６３）,此值较健侧升高（３８６３±３７１）％。结论电针治疗可以提高大鼠实验性偏侧ＰＤ模型纹状体ＤＡ含量,并可抑制早期实验性ＰＤ时Ｄ２受体的上调效应     

Knee injury and Osteoarthritis Outcome Score (KOOS) - validation of a Swedish version

The Knee injury and Osteoarthritis Outcome Score (KOOS) is a self-administered instrument measuring outcome after knee injury at impairment, disability, and handicap level in five subscales. Reliability, validity, and responsiveness of a Swedish version was assessed in 142 patients who underwent arthroscopy because of injury to the menisci, anterior cruciate ligament, or cartilage of the knee. The clinimetric properties were found to be good and comparable to the American version of the KOOS. Comparison to the Short Form-36 and the Lysholm knee scoring scale revealed expected correlations and construct validity. Item by item, symptoms and functional limitations were compared between diagnostic groups. High responsiveness was found three months after arthroscopic partial meniscectomy for all subscales but Activities of Daily Living.     

Endovascular management of carotid-cavernous fistulas

Objective To investigate endovascular treatment of traumatic direct carotid-cavernous fistulas （CCF） and their complications such as pseudoaneurysms. Methods： Over a five-year period, 22 patients with traumatic direct CCFs were treated endovascularly in our institution. Thirteen patients were treated once with the result of CCF occluded, 8 twice and 1 three times. Treatment modalities included balloon occlusion of the CCF, sacrifice of the ipsilateral internal carotid artery with detachable balloon, coll embolization of the cavernous sinus and secondary pseudoaneurysms, and covered-stem management of the pseudoaneurysms. Results All the direct CCFs were successfully managed endovascularly. Four patients developed a pseudoaneurysm after the occlusion of the CCF with an incidence of pseudoaneurysm formation of 18.2% （4/22）. A total number of 8 patients experienced permanent occlusion of the ICA with a rate of ICA occlusion reaching 36.4% （8/22）. Followed up through telephone consultation from 6 months to 5 years, all did well with no recurrence of CCF symptoms and signs. Conclusion Traumatic direct CCFs can be successfully managed with endovascular means. The pseudoaneurysms secondary to the occlusion of the CCFs can be occluded with stent-assisted coiling and implantation of covered stents.     

The acutely limping child

Acute limping may be the result of multiple pathologies in children. The differential diagnosis varies based on the age of the child. Irrespective of age, the initial imaging work-up includes AP and frog leg radiographs of the pelvis and ultrasound; MRI may sometimes be helpful. In children less than 3 years, infections and trauma are most frequent. MRI is the imaging modality of choice when osteomyelitis is clinically suspected. Between the ages of 3 and 10 years, transient synovitis of the hip and Legg-Calvé-Perthes disease are main considerations but infection, inflammation and focal bony lesions are also considered. In children over 10 years, slipped capital femoral epiphysis also is considered.     

Do Balance Board Training Programs Reduce the Risk of Ankle Sprains in Athletes?

Introduction Ankle sprains are the most common musculo-skeletal injury that occurs in athletes,particularly in sports that require jumping and landing on one foot such as soccer,and basketball(1-4).These injuries often result in significant time loss from participation,long-term disability,and have a major impact on health care costs and resources(5-8).     

High Intensity Interval Training:New Insights

KEY POINTS ·High-intensity interval training(HIT)is characterized by repeated sessions of relatively brief，intermittent exercise.often performed with an“a11 out”effort or at an intensity close to that which elicits peak oxygen uptake(i.e.，≥90%of VO2 peak).     

Developmental validation of the PowerPlex(®) ESI 16 and PowerPlex(®) ESI 17 Systems: STR multiplexes for the new European standard

In response to the ENFSI and EDNAP groups’ call for new STR multiplexes for Europe, Promega^® developed a suite of four new DNA profiling kits. This paper describes the developmental validation study performed on the PowerPlex^® ESI 16 (European Standard Investigator 16) and the PowerPlex^® ESI 17 Systems. The PowerPlex^® ESI 16 System combines the 11 loci compatible with the UK National DNA Database^®, contained within the AmpFlSTR^® SGM Plus^® PCR Amplification Kit, with five additional loci: D2S441, D10S1248, D22S1045, D1S1656 and D12S391. The multiplex was designed to reduce the amplicon size of the loci found in the AmpFlSTR^® SGM Plus^® kit. This design facilitates increased robustness and amplification success for the loci used in the national DNA databases created in many countries, when analyzing degraded DNA samples. The PowerPlex^® ESI 17 System amplifies the same loci as the PowerPlex^® ESI 16 System, but with the addition of a primer pair for the SE33 locus. Tests were designed to address the developmental validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM), and those of the DNA Advisory Board (DAB). Samples processed include DNA mixtures, PCR reactions spiked with inhibitors, a sensitivity series, and 306 United Kingdom donor samples to determine concordance with data generated with the AmpFlSTR^® SGM Plus^® kit. Allele frequencies from 242 white Caucasian samples collected in the United Kingdom are also presented. The PowerPlex^® ESI 16 and ESI 17 Systems are robust and sensitive tools, suitable for the analysis of forensic DNA samples. Full profiles were routinely observed with 62.5 pg of a fully heterozygous single source DNA template. This high level of sensitivity was found to impact on mixture analyses, where 54–86% of unique minor contributor alleles were routinely observed in a 1:19 mixture ratio. Improved sensitivity combined with the robustness afforded by smaller amplicons has substantially improved the quantity of data obtained from degraded samples, and the improved chemistry confers exceptional tolerance to high levels of laboratory prepared inhibitors.