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用抗早孕实验和大鼠子宫离体实验,研究了醋酸棉酚与米索前列醇抗早孕的协同作用。结果表明,早孕小鼠单服米索,其终止早孕作用微弱;当与棉酚合用时,对小鼠的抗早孕作用增强。对离体大鼠子宫,米索有明显的增强宫缩作用,而棉酚则无影响,但早孕大鼠po棉酚80mg·kg-1·d-1,3d后其子宫对米索的敏感性较对照组有显著提高。大鼠于妊娠d 6~8 po棉酚或米索,或两药剂量的一半合并用药,可使子宫蜕膜组织损伤,而以两药合用组最为严重,但子宫孕酮受体含量和分布与对照组相似。结果提示,两药合用有协同抗早孕作用。 相似文献
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观察棉酚合并 PG0 5对小鼠的抗早孕作用。结果显示 :单用棉酚 75mg/( kg·d) ,3d无抗早孕作用 ,而同样剂量棉酚与 PG0 50 .2 mg/( kg·d) ,合用 2 d可显著增强抗早孕作用 ,使小鼠活胎数进一步减少 ;棉酚 30 μg/ml和 ( - )棉酚 1 5μg/ml对大鼠黄体细胞 3β- HSD活性均有显著抑制作用 ,( + )棉酚 1 5μg/ml和PG0 51 0 μg/ml对此酶活性无影响 ;( - )棉酚 1 5μg/ml还能抑制大鼠颗粒细胞雌二醇的分泌 ,提示棉酚增强 PG0 5抗早孕作用可能是从多环节影响胚胎正常发育。 相似文献
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盐酸苯乙哌啶(R1132)10μg/ml或dl-15甲基PGF_(2α)甲酯(PG05)5或10μg/ml在体外能明显抑制黄体细胞对hCG的反应性,使孕酮分泌下降。假孕大鼠po R1132 10 mg/kg或Sc PG 05 5.1 mg/kg不影响卵巢孕酮分泌,合并给药后却能使其降低。R1132无抗孕酮作用。卵巢分泌孕酮减少可能是抗早孕的主要原因.假孕大鼠po R1132 50 mg/kg或sc PG050.5 mg/kg可抑制卵巢腺苷环化酶的活性.该酶可能是R1132或PG05在大鼠抗早孕作用的重要靶酶。 相似文献
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盐酸苯乙哌啶(R1132)10μg/ml或dl-15甲基PGF2α甲酯(PG05)5或10μg/ml在体外能明显抑制黄体细胞对hCG的反应性,使孕酮分泌下降。假孕大鼠po R1132 10 mg/kg或Sc PG 05 5.1 mg/kg不影响卵巢孕酮分泌,合并给药后却能使其降低。R1132无抗孕酮作用。卵巢分泌孕酮减少可能是抗早孕的主要原因.假孕大鼠po R1132 50 mg/kg或sc PG050.5 mg/kg可抑制卵巢腺苷环化酶的活性.该酶可能是R1132或PG05在大鼠抗早孕作用的重要靶酶。 相似文献
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(+),(-)和(±)棉酚在雌大鼠抗早孕作用的研究 总被引:1,自引:0,他引:1
妊娠第6~9天大鼠,分别ig(±)和(-)棉酚80mg·kg-1·d-1和40mg·kg-1·d-1,结果有明显的抗早孕作用。然而(+)棉酚40mg·kg-1·d-1对大鼠生育无明显影响。(-)棉酚30μg·ml-1能抑制体外培养黄体细胞孕酮的分泌。(+)棉酚10μg·ml-1能促进黄体细胞分泌孕酮。hCG1IU·ml-1能明显刺激体外培养颗粒细胞孕酮的分泌。(±)棉酚10和30μg·ml-1皆能明显降低颗粒细胞对hCG的反应性。 相似文献
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Racemic, (-) and (+) gossypol, provided by the Department of Organic Chemistry of our institute, was suspended in 2.5% tween 80 solution. Adult male Wistar rats 190~220 g in weight were allotted to 5 groups. Animals in group 1 received 2.5% tween 80 solution as control. Rats in group 2 were treated with racemic gossypol at the dosage of 30 mg/kg for 2 weeks. Animals in group 3 and 4 were given 15 mg/kg of (-) gossypol for 2 weeks and 30 mg/kg of (-) gossypol for 1 week respectively. Rats in group 5 were treated with (+) gossypol at the dosage of 30 mg/kg for 2 weeks.Four weeks from the beginning of gossypol treatment the rats were cohabited with adult females for 7 days. Then the motility of the sperms in the cauda epididymides was estimated The female rats were examined for pregnancy 7 days later.Treatment with (-) gossypol at 30 mg/kg caused significant decreases in body weight of the rats (P<0.05). One of the five rats died 7 days after the last administration, while (+) gossypol and racemic gossypol at the dosage employed had no effect on the body weight. (+) Gossypol at 30 mg/kg for 2 weeks had no effect on the motility of the sperms in the cauda epididymides and no effect on the fertility of the animals: nor was there any effect on the weights of the testis, epididymis, prostate and seminal vesicle. The sperms of the cauda epididymides were found to be dead in the groups treated with 15 and 30 mg/kg of (-)gossypol. Raccmic gossypol given for 2 weeks at 30 mg/kg caused loss of fertility of the male rats which confirmed our previous findings.(?)t may be postulated that (+) gossypol has no antifertility effect nor toxicity at the dosage employed. (-) Gossypol is the active stereoisomer of racemic gossypol. 相似文献
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S Kai H Kohmura K Ishikawa Y Makihara S Ohta S Kawano N Takahashi 《The Journal of toxicological sciences》1989,14(2):115-130
In the preceding study, no teratological effects against rat fetuses were observed when carboplatin, an oncostatic platinum coordination complex, was dosed to their dams from days 7 to 17 of gestation at a dose level of 4 mg/kg/day. However, there are a few reports which show the teratogenic action of carboplatin injected from days 6 to 15 of pregnancy at a dose level of 6 mg/kg/day. In the present study, carboplatin was administered intravenously to pregnant female Crj: CD (Sprague-Dawley) rats from days 6 to 9 or 7 to 10 of gestation at a dose level of 6 mg/kg/day in order to know the teratogenicity of this drug. Carboplatin were highly embryolethal in dams when dosed from days 6 to 9 of gestation, but not in animals when injected from days 7 to 10 of pregnancy. Carboplatin also produced external, internal and skeletal anomalies in fetuses such as gastroschisis, dilatation of cerebral ventricles, cleft sternum, fused ribs, malformed thoracic vertebra when administered from days 6 to 9 of gestation, but not in conceptuses when dosed from days 7 to 10 of pregnancy. However, the delayed ossification which was ascribed to the fetal growth retardation was observed in rats treated with this drug during both administration periods. These results suggest that carboplatin is embryotoxic, inducing intrauterine death and congenital malformations in rats, when injected during the early stages of gestation including day 6 of pregnancy. 相似文献
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醋酸棉酚有抗雄性生育作用,但对睾丸间质细胞的作用尚未取得一致意见。部分研究者观察到醋酸棉酚对睾丸间质细胞有抑制作用。本室也曾证明,服醋酸棉酚后,青春前期大鼠,睾丸间质细胞的生长发育及其类固醇激素合成酶的活性均受抑制。已有报告,睾丸间质细胞表面具有分子量为20万的LH/hCG受体,该受体的数量及活性决定了间质细胞睾酮分泌活性及对促激素的反应。此受体在大鼠五周龄前逐步发育完善,而在4周龄时已表现出对外源性LH/hCG敏感的反应性。本实验以幼龄大鼠为对象,用放射免疫测定及放射受体分析法研究了醋酸棉酚对睾丸间质细胞的影响。 相似文献
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Previously, we demonstrated that bromodichloromethane (BDCM), a drinking water disinfection by-product, causes pregnancy loss in F344 rats when given on gestational days (GD) 6-10, encompassing the luteinizing hormone (LH)-dependent period of pregnancy (GD 7-10). Pregnancy loss, i.e., full-litter resorption, was associated with reduced serum progesterone levels; however, we were unable to identify an effect on serum LH. Here, we reevaluated serum LH levels using the more sensitive technique, DELFIA(R). We further sought to better define the temporal pattern of endocrine disruption caused by BDCM during pregnancy with more frequent sampling. Lastly, we attempted to prevent BDCM-induced pregnancy loss using exogenous progesterone or human chorionic gonadotropin (hCG), an LH-agonist. BDCM, in 10% Alkamuls(R), was dosed at 75 mg/kg/day by gavage to F344 rats on GD 6-10 (plug day = GD 0). BDCM-induced pregnancy loss was associated with marked reductions in serum progesterone and LH on GD 10. The decrease in serum LH consistently preceded the decrease in progesterone. In the hormone replacement studies, BDCM and progesterone were administered on GD 6-10, hCG on GD 8-10. BDCM was delivered at 100 mg/kg/day, progesterone at 10 mg/kg twice daily, and hCG at 0.5 IU/0.2 ml/rat. Both progesterone and hCG prevented BDCM-induced pregnancy loss. Thus, BDCM-induced pregnancy loss was associated with marked GD-10 reductions in serum LH and corresponding decreases in progesterone. Furthermore, coadministration of an LH agonist prevented pregnancy loss, supporting the hypothesis that BDCM-induced pregnancy loss in the rat occurs via an LH-mediated mode of action. 相似文献
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Martín Palomar-Morales Luis A. Baiza Leticia Verdín-Terán Rubén Román-Ramos Mario Altamirano-Lozano José D. Méndez 《Reproductive toxicology (Elmsford, N.Y.)》1998,12(6):153-665
The effect of alloxan on embryo and fetal development in rats was evaluated. Alloxan was injected intraperitoneally (ip) in pregnant rats at doses of 80 to 150 mg/kg at Day 0 (day of fertilization), and 110 mg/kg at Day 4 of pregnancy. Hyperglycemia was rarely produced at alloxan doses from 80 to 100 mg/kg, and the frequency of malformations observed was low. Higher doses (110 to 150 mg/kg) caused severe hyperglycemia, and maternal or embryonic death. When 110 mg/kg was administered on Day 4 of gestation (the day before embryo implantation), all rats had resorption nodules and litters with embryos with delayed growth. We recommend the induction of diabetes mellitus on Day 4 of pregnancy for studies of diabetes–gestation interaction. 相似文献