Sex differences in expression and differential regulation by androgen and estrogen of two odorant-binding tear lipocalins in lacrimal glands of immature hamsters

In adults of several mammalian species, lacrimal glands (LG) have sex differences but there is no report of any sexual dimorphism in LG of immatures. In LG and tears of adult hamsters, we found female-specific expression of two closely related odorant-/pheromone-binding lipocalins, FLP (female lacrimal protein) and MSP (male-specific protein; initially identified in salivary glands of males). Although, both androgens and estrogens markedly repress FLP and MSP in LG of adults, the expression of these lipocalins in females is due to their incomplete repression by endogenous estrogens. Here we report a marked sexual dimorphism in the expression of FLP and MSP in LG and tears of 20-day-old immature hamsters. The age-dependant expression of these lipocalins and effect of neonatal-gonadectomy and sex hormone treatments on their expression in immatures was investigated. FLP and MSP are detectable in LG at 10-day age in both sexes of hamster but by 20-day age levels of both lipocalins show sex differences wherein FLP is several fold higher in males and MSP is obliterated in males. Thereafter, FLP declines in male LG and is obliterated by 36-day age, resulting in female-specific expression of both LG lipocalins as seen in adults. In LG of 20-day-old immatures, FLP and MSP are insensitive to repression by androgen and estrogen, respectively, which was unlike the androgen/estrogen-repressed regulation of both lipocalins in adult LG. The estrogenic repression of FLP and androgenic repression of MSP in LG of immature hamsters could be prevented by treatment with tamoxifen and flutamide, respectively. Our studies indicate that (i) presence of gonads in immatures can have significant effects on LG lipocalins resulting in their sexually dimorphic expression, (ii) in immatures, unlike adults, the repressive effects of estrogen and androgen on LG lipocalins are selective for FLP and MSP, respectively, and (iii) these repressions are likely to be mediated by sex hormone receptors.     

Chronic administration of melatonin induces changes in porphyrins and in the histology of male and female hamster Harderian gland: Interrelation with the gonadal status

In this paper, we have investigated the influence of melatonin on the histology and porphyrin content of the Syrian hamster Harderian glands. Daily afternoon injections of 25 micrograms of melatonin to female hamsters for 12 weeks resulted in the discontinuity of estrous cyclicity, a marked decrease in the Harderian gland intraluminal area occupied by porphyrins, and in a significant rise in the number of Type II cells. A similar decrease in porphyrins was observed after 8 weeks of ovariectomy. However, if the melatonin injections were given for only 8 weeks (without inducing gonadal atrophy), no changes were observed in the area occupied by intraluminal porphyrins, suggesting that the effects of melatonin in female Syrian hamsters might be associated with the subsequent gonadal atrophy. Castration of male hamsters induced a significant increase in porphyrins and a clear drop in the number of Type II cells. These changes were totally prevented when melatonin was administered daily from the day of castration. Our results suggest that melatonin, at least in male Syrian hamsters, plays a role in Harderian metabolism, acting directly on the Harderian secretory cells or indirectly through pituitary hormones.     

Regulation of the androgen receptors in the Harderian gland of the male Syrian hamster: Influence of photoperiod, castration, and chronic melatonin treatment

Male Syrian hamsters that were exposed for 8 weeks to short photoperiod (LD 10:14) or treated with melatonin in the late afternoon under long photoperiod conditions (LD 14:10) had a significantly higher content of androgen receptors in the Lipidex-purified soluble fractions isolated from the Harderian glands as compared to the long photoperiod (LD 14:10) exposed controls. Simultaneous computer-assisted analyses of all series of saturation and competition experiments revealed that the numerical value of the apparent Kd, as determined by using the synthetic androgen R-1881 (methyltrienolone), was not different between the experimental groups, and ranged from 0.050 to 0.067 nM. Of the principal natural androgens, testosterone (T) was most potent in inhibiting methyltrienolone binding to the receptor (Ki values from 0.33 to 0.55 nM), and 5 alpha-dihydrotestosterone (DHT) and delta 4-androstenedione (AD) were less effective (Ki values between 1 and 1.9 nM). In the hypothalami and pituitaries of the same animals, used in parallel control assays, DHT was twice as potent as T. Short-term castration (24 hr post-orchidectomy) did not result in significant changes in the receptor binding characteristics. Following 8 weeks exposure to a long photoperiod (LD 14:10) the Bmax values demonstrated a four-fold increase in castrated animals (179 fmoles/mg protein vs. 47 fmoles/mg protein) over intact controls. The relative binding affinity of the major androgens under these conditions remained unchanged, with the exception of AD, where a five-fold increase in the numerical Ki values (decrease in the binding affinity) was recorded (Ki = 9.6 nM).(ABSTRACT TRUNCATED AT 250 WORDS)