免疫透射比浊速率法测定IgG

为克服免疫透射比浊终点法测定特定蛋白存在的某些不足 ,特别是因抗原过剩引起的假性低结果。探讨用免疫透射比浊速率法测 Ig G,以求减少这种偏差。结果表明 ,本法精密度良好 ,批内、批间 CV分别为 3.5 4%和4.2 6 % ;线性范围宽 ,上限可达 72 .5 g/ L ;与免疫透射比浊终点法相比 ,约可减少 5 0 %因抗原过剩引起的假性低结果。本法与仪器配套进口试剂对 10 0例样品进行对比试验 ,结果相关良好 ,r=0 .995 ,回归方程 Y=0 .995 x- 0 .0 5 9     

免疫透射比浊测定脑脊液免疫球蛋白

目的　建立一种适合于全自动生化分析仪检测的脑脊液免疫球蛋白 (Ig)测定方法。方法　对Roche血清Ig测定试剂盒分析参数进行修改 ,主要是降低校准液浓度、减少加入的试剂抗体量、增加检测的标本量 ,改变测定波长。共检测脑脊液标本 4 0例 ,并与散射比浊法进行对比研究。结果　透射比浊法测定校准品及脑脊液IgG、IgA、IgM低浓度标本其批内与日间CV均 <14 % ,而在高浓度标本则 <6 % ,与散射比浊法相比其相关系数分别为 0 .9933、0 .9982、0 .996 0 ,回归方程分别为Y =1.0 1X - 9、Y =1.13X - 0 .1、Y =0 .96X 0 .2。结论　本研究为脑脊液Ig的测定提供了一种简便、快速、实用的自动化分析方法。     

免疫透射比浊测定脑脊液免疫球蛋白

目的建立一种适合于全自动生化分析仪检测的脑脊液免疫球蛋白(Ig)测定方法.方法对Roche血清Ig测定试剂盒分析参数进行修改,主要是降低校准液浓度、减少加入的试剂抗体量、增加检测的标本量,改变测定波长.共检测脑脊液标本40例,并与散射比浊法进行对比研究.结果透射比浊法测定校准品及脑脊液IgG、IgA、IgM低浓度标本其批内与日间CV均<14%,而在高浓度标本则<6%,与散射比浊法相比其相关系数分别为0.993 3、0.998 2、0.996 0,回归方程分别为Y=1.01X-9、Y=1.13X-0.1、Y=0.96X+0.2.结论本研究为脑脊液Ig的测定提供了一种简便、快速、实用的自动化分析方法.     

免疫透射比浊法测定类风湿因子的应用评价

目的建立免疫透射比浊法测定类风湿因子（RF）的方法。方法用免疫透射比浊法测定RF，并对其重复性、准确性、线性范围、相关性实验、干扰实验加以评价。结果高、中、低3种浓度RF批内CV分别为0．9％、0．9％、0．0％；批间CV分别为3．2％、3．6％、3．8％；线性范围为0-200U／L；平均回收率为100．2％，与特种蛋白仪测定结果具有很好的相关性（P〈0．05）。一定程度的溶血、脂血、黄疸对RF的测定无明显干扰。结论免疫透射比浊法的精确度好、快速、灵敏，与免疫散射比浊法有很好的相关性，可以作为测定RF的常规方法。     

颗粒增强免疫透射比浊法测定甲胎蛋白方法学评价

目的对定量测定甲胎蛋白(AFP)的颗粒增强免疫透射比浊法(PETIA)进行方法学评价。方法应用OLYMPUS AU5400全自动生化分析仪定量测定AFP,探讨该检测方法的精密度、试剂开瓶稳定性、检测下限、敏感性、线性范围、干扰和准确性;并与SIEMENS CENTAUR电化学发光法(ECLIA)定量测定临床新鲜血清AFP的结果进行相关性分析,同时对其参考区间进行验证。结果 29.95和132.25 ng/mL的新鲜血清测定的批内变异系数(CV)分别为1.67%和1.52%;天间CV分别为7.17%和7.84%;测定下限和敏感性分别为0.52和0.53 ng/mL;5.4～256 ng/mL范围内其测定的线性相关系数(r2)达0.999 4,回归方程为Y=1.014X+1.063 3;高、低水平浓度的定值质控血清测定的相对偏差分别为5.96%和8.40%,远小于卫生部临床检验中心AFP室间质评能力比对试验(PT)评价标准的"靶值±20%";107例临床病例测定结果与SIEMENS化学发光分析仪测定的结果相关性良好,YECLIA=1.138 7XPETIA+2.835 3(r2=0.992 1);一定程度的溶血、黄疸及脂血对该测定无明显干扰;20名健康体检者AFP测定结果均处于厂家推荐的参考区间内。结论 PETIA测定AFP可作为一种简便易行、快捷价廉、准确可靠的临床常规检测方法,特别适合于健康体检时大规模筛查。     

免疫透射比浊法与免疫散射比浊法检测特定蛋白的比较

目的对免疫透射比浊法与免疫散射比浊法两种方法检测特定蛋白进行比较。方法应用Olymous-AU2700全自动生化分析仪与DadeBehring BNProspec特定蛋白仪对IgG、IgM、IgA、C3和C45种血清蛋白进行初步的方法学比较。结果免疫透射比浊法与免疫散射比浊法的批内精密度均较好,CV<3.3%。免疫透射比浊法与免疫散射比浊法在医学决定水平浓度从低检测范围到高检测范围,均有良好的线性回归,相关系数分别为:0.96、0.96、0.90、0.98、0.84。结论免疫透射比浊法具有较好的精密度及较宽的检测范围,与免疫散射比浊法相关性良好,且透射比浊法成本低,收费低,便于基层及常规实验室开展。     

免疫透射比浊法测定尿免疫球蛋白G的建立与评价

免疫球蛋白G（IgG）是血清中含量较多的一种大分子免疫球蛋白（分子量为16KD），主要由脾及周围淋巴浆细胞合成，通常不易通过肾小球滤过膜，尿中排量极少。一般在肾小球病变较重时免疫球蛋白G才排出显著增加。尿中免疫球蛋白G增多与肾小球病变严重程度有关．大都是非选择性蛋白尿。     

免疫透射比浊法测定纤维蛋白原标准曲线拟合公式的选定

本文采用手工操作,以免疫透射比浊法(波长 405nm)绘制纤维蛋白原标准工作曲线,用浓度(X)和相应吸光度(Y)对6种不同回归方程进行验证,比较其准确性.结果表明,纤维蛋白原用方程lgY=a +blgx计算较为合适.     

免疫透射比浊法测定尿免疫球蛋白G的建立与评价

免疫球蛋白G（IgG）是血清中含量较多的一种大分子免疫球蛋白（分子量为16KD），主要由脾及周围淋巴浆细胞合成，通常不易通过肾小球滤过膜，尿中排量极少。一般在肾小球病变较重时免疫球蛋白G才排出显著增加。尿中免疫球蛋白G增多与肾小球病变严重程度有关．大都是非选择性蛋白尿。     

免疫透射比浊法定标方式的探讨

目的研究不同的定标方式对免疫透射比浊法测定免疫球蛋白、补体结果的影响。方法使用OLYMPUS AU640自动生化分析仪和立德曼免疫透射比浊法试剂,采用不同的定标方法对40例血清的IgG,IgA,IgM,C3和C4进行测定。结果使用多标准POLYGONAL和SPLINE方法拟合定标曲线后测定结果表现良好的一致性,多点定标与单点定标测定结果具有显著性差异。结论免疫透射比浊测定,应当根据仪器情况,选择适当的非线性曲线拟合方式建立标准工作曲线。     

导数同步荧光法测定血清色氨酸

为了排除酷氨酸、苯丙氨酸对荧光法测定色氨酸的干扰，建立了色氨酸导数－同步荧光测定法。结果表明，当△λ为９０ｎｍ时，色氨酸的一个阶导数－同步荧光峰在２８５ｎｍ时不受酪氨酸、苯丙氨酸的干扰；色氨酸浓度在０～４００μｍｏｌ／Ｌ与导数－同步荧光值成线性，Ｙ＝０．２８７Ｘ±０．０９６，ｒ＝０．９９９；批内、批间ＣＶ分别为２．５７％、３．４２％；回收率９３．８％～９７．２％；血红蛋白含量在０．２５～５．０ｇ／     

过敏性紫癜患儿食物不耐受检测及其意义

目的探讨过敏性紫癜患儿食物不耐受血清特异性IgG水平对其病因诊断及饮食治疗的意义。方法用食物不耐受IgG体外检测试剂盒检测30例过敏性紫癜患儿和20例健康对照儿童的血清特异性IgG水平。结果实验组30例过敏性紫癜患者的血清总IgG阳性率为76.67%,其中对1种食物不耐受为3例,对2种以上食物不耐受者为20例。健康对照组20例血清总IgG阳性率为25%,对1种食物不耐受为2例,对2种以上食物不耐受者为3例,常见的引起不耐受的食物依次为牛奶、虾、鸡蛋清和鸡蛋黄、蟹、大豆、鳕鱼、大米、小麦、猪肉、牛肉等,实验组饮食上经避免不耐受食物及抗过敏治疗疗程缩短,疗效优于对照组。结论血清特异性IgG水平检测对过敏性紫癜的病因诊断有参考意义,对患儿饮食治疗及营养状态的改善具有重要的指导意义。     

遗传性血管性水肿患者C1INH和C4测定结果

目的评估速率散射比浊法测定C1INH和C4的临床应用价值。方法应用速率散射比浊法测定60份(HAE23例,正常人37例)血清样本,并与免疫单扩散法比较。结果HAE患者的C1INH和C4水平与正常对照组比较有统计学显著性差异(P<0.01),两种方法测定结果的相关性良好(P<0.01)。速率散射比浊法批内变异系数小于4.6%,批间变异系数小于4.9%。结论速率散射比浊法测定血清C1INH和C4含量结果可靠,操作简便,适合于临床应用。     

Determination of localization of allergen and IgG inducing antigen in Dirofilaria immitis by immunofluorescent antibody method

More detail studies were made into the localization of IgE inducing antigen (allergen) and IgG inducing antigen in the body of adult male and female Dirofilaria immitis worms by the indirect immunofluorescent antibody method using rat sera against purified allergen and IgG inducing antigen. Allergen was detected in large amounts in the body cavity fluid and the excretory canal, and it was also detected in the cuticles and the lateral lines in the vicinity of the excretory canal. IgG inducing antigen was found in the largest amount in the site of musculature inside of cuticle. Besides the musculature, it was also recognized in smaller amounts in the muscle layer, the body cavity fluid and the excretory canal. In females it was also detected on the surface of microfilaria in the uterus. As to allergen as well as IgG inducing antigen, the fluorescence was recognized most strongly in the central part of the worm body, and it tended to diminish gradually as it approached the head and tail. The concentration of allergen determined by the immunofluorescent antibody method was higher in males than in females. Especially in the excretory canal, a difference was seen between males and females. Allergen was thought to be secreted mainly out of the excretory canal and present largely in the body cavity fluid. On the other hand, the site of the musculature inside of cuticle and the muscular layer were supposed to be the major constituents of IgG inducing antigen.     

Determination of creatinine in serum and urine by a rapid liquid-chromatographic method

We describe an HPLC ion-pair procedure for rapid and specific evaluation of creatinine in serum and urine. We used a 15 cm X 4.6 mm ODS column with a 50/50 (by vol) mixture of sodium decanesulfonic acid (10 mmol/L, pH 3.2) and methanol and measured absorbance at 236 nm. Serum (100 microL) or 30-fold-diluted urine (100 microL) was added to 400 microL of acetone. After centrifugation, the supernates (300 microL) were dried, reconstituted with the mobile phase, and injected into the HPLC. Assay precision was tested for concentrations of 10, 29, and 130 mg/L and yielded, respectively, 3.1%, 2.1%, and 1.1% for within-day CV and 2.8%, 2.1%, and 2.2% for total CV. Analytical recovery was 102 (+/- 6.7%). Linearity was demonstrated in the 0-200 mg/L range for serum and 0-3.5 g/L range for urine (r greater than or equal to 0.999). The detection limit for creatinine (signal-to-noise ratio = 3) was 0.5 mg/L. We used cimetidine for internal standardization. Correlation was good between this procedure and the Jaffé kinetic, the enzymatic (creatinine amidohydrolase), and the Fuller's earth alkaline picrate methods.     

Determination of serum triglycerides by an accurate enzymatic method not affected by free glycerol

In this automated single-run enzymatic procedure for specific determination of triglycerides in serum, free glycerol is removed from the reaction mixture by pre-incubation with glycerol phosphate oxidase and peroxidase. The subsequent addition of lipase and the chromogen, 4-aminoantipyrine, results in the formation of color proportional to the amount of triglycerides in serum. Standards containing triolein in aqueous detergent are used to calibrate the method. For serum pools from the Centers for Disease Control with target values of 0.74, 1.41, and 2.63 mmol/L, the method produced biases of +0.01, -0.05, and 0.00 mmol/L, respectively (mean: -0.01 mmol/L or -0.4%). The mean coefficient of variation was 1.4% within and 2.5% between days; the combined CV, 2.9%. Ninety 6-microL serum samples can be analyzed per hour. The method is more accurate and precise than one based on an NADH-coupled enzyme system with separate addition of lipase.     

采用离心浓集法提高乙型肝炎病毒表面抗原检出率的可行性研究

目的 探讨离心浓集法预处理血清标本对提高乙型肝炎血清学标志物乙型肝炎表面抗原(HBsAg)检出率的可行性研究.方法 收集广州市内各大医院2009年52例HBsAg阳性者和疑似乙型肝炎感染者血清标本,分别用酶联免疫吸附试验(ELISA) 和化学发光免疫分析法(CLIA)测定HBsAg.再以PEG6000为沉淀剂,将标本进行高速离心浓集(30 000 r/min)25 ℃,40 min,取沉淀用TE Buffer溶解,采用上述相同方法分别进行测定,比较离心前后其HBsAg 的S/CO值与HBsAg定量值的上升情况,用SPSS统计学软件对数据进行非参数检验,分析其差别是否有统计学意义.结果 健康对照组与空白对照经高速离心处理前后的HBsAg 检测结果差异均无统计学意义(P>0.05),而疑似HBV感染组和HBV感染组患者血清经高速离心预处理后的测定结果均明显高于处理前(P<0.01).结论 离心浓集法预处理对于血清中有低水平乙型肝炎病毒颗粒的感染者有效,血清中的病毒颗粒可通过高速离心处理后得到浓缩,提高临床实验室常规诊断手段的灵敏度的范围,使隐匿型乙型肝炎的检出率有所提高.     

Enzymatic fluorometric method for the determination of D-arabinitol in serum by initial rate analysis

We describe a new, simple, fluorometric assay for D-arabinitol in serum. The method is based on oxidation of D-arabinitol by D-arabinitol dehydrogenase (EC 1.1.1.11), with the concomitant reduction of NAD. The initial rate of NAD reduction, which is proportional to the D-arabinitol content of serum, can be measured with a recording spectrofluorometer. Sensitivity, specificity, recovery and reproducibility experiments gave satisfactory results. The proposed method is suitable for clinical use, and may be helpful in the diagnosis of invasive candidiasis.