高锌摄入对机体血脂脂蛋白及载脂蛋白代谢的影响

目的 :观察健康人高锌摄入对血脂、脂蛋白及载脂蛋白代谢的影响 ,为合理用锌提供依据。方法 :选择长清县五峰乡当地居民 38例 ,服葡萄糖酸锌 (0 .2 g ,含锌 5 0mg/d) ,每次专人送药 ,连服 8周。在服药前、服药后 4、8周 3次查体 ,描记心电图 ,测定血压。 3次查体同时取静脉血 ,测定胆固醇 (TC)、甘油三酯 (TG)、低密度脂蛋白胆固醇 (LDL -C)、高密度脂蛋白胆固醇 (HDL -C)、载脂蛋白AI(apoA1)、载脂蛋白B10 0(apoB10 0 ) )含量 ,观察动态变化。结果 :服锌 4周时TC升高 ,8周持续升高 ,TG 4周时升高 ,8周时处稳定状态 ,8周时LDL -C升高 ,4周时apoB10 0升高 ,8周时apoA1降低 ,apoA1/apoB10 0值变小。结论 :健康人摄入锌 5 0mg/d时 ,引起脂类代谢紊乱 ,临床应谨慎使用锌制剂。     

高锌摄入对机体代谢的影响

目的　观察健康人高锌摄入对机体锌铜及脂类代谢和抗氧化功能的影响 ,为合理补锌提供依据。方法　选择农村健康居民 4 0例 ,每天补锌 5 0mg(0 2g葡萄糖酸锌 ) ,连服 8周。在服锌前和服锌后 2、4、8周及停锌后 4周 5次查体、取血并测定各项有关指标。结果　①血清、红细胞和发锌含量在服锌 2～ 4周后明显升高 ,停锌 4周后虽然下降但仍高于服锌前水平 ;2 4h尿锌排出量服锌后明显升高 ,停锌后即降低到服锌前水平。②血清、红细胞和发铜含量在服锌 4周后明显升高 ,停锌后铜含量降低 ;2 4h尿铜排出量服锌前后和停锌后无差异。③红细胞超氧化物歧化酶 (SOD)活性在服锌 2周后即持续降低 ,停锌 4周时未能恢复 ;红细胞谷胱甘肽过氧化物酶 (GPX)活性在服锌 4周时明显升高 ;脂质过氧化物 (LPO)含量在服锌 2周后升高。④服锌后总胆固醇 (TC)、甘油三酯 (TG)和低密度脂蛋白胆固醇 (LDL C)升高 ;高密度脂蛋白胆固醇 (HDL C)降低 ;载脂蛋白A1(apoA1)降低 ;载脂蛋白B1 0 0 (apoB1 0 0 )升高。结论　健康人每天补充 5 0mg锌会干扰锌铜代谢 ,抑制抗氧化功能 ,引起脂类代谢紊乱。     

2型糖尿病人群脂代谢紊乱与血清尿酸水平变化的相关性分析

目的:探讨2型糖尿病(T2DM)人群脂代谢紊乱与血清尿酸(SUA)水平变化的相关性.方法:88例健康体检者为对照组,110例经临床确诊T2DM患者为观察组,采用全自动生化分析仪检测血清甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL—C)、高密度脂蛋白胆固醇(HDL— C)、血清载脂蛋白Al (ApoAl)、载脂蛋白B(ApoB)、ApoAl/ApoB、HDL—C/LDL—C、HDL— C/TC及血清SUA浓度,并进行统计学分析.结果:与对照组比较,观察组人群TG、TC、LDL—C、ApoB、SUA水平明显升高,HDL—C、ApoAl、ApoAl/ApoB、HDL—C/LDL—C、HDL—C/TC浓度明显降低;差异均有统计学意义(P＜0.01或P＜0.05);经多元回归分析:T2DM人群血清TG、TC、LDL—C、ApoB与SUA水平呈正相关;HDL—C、ApoAl与SUA水平呈负相关.结论:T2DM人群脂质代谢发生紊乱,血清TG、TC、LDL—C、ApoB、SUA水平明显升高,HDL—C、ApoAl、ApoAl/ApoB、HDL—C/LDL—C、HDL—C/TC浓度明显降低;其脂代谢紊乱及SUA增高是造成动脉粥样硬化(AS)危险因素.     

铜摄入不足大鼠血中铜含量及铜锌比值对血脂的影响

目的 探讨在铜摄入量低于正常摄入量的情况下血中铜、锌含量、铜,锌比值及血脂的相关关系,为铜的多途径暴露提供统一的剂量依据.方法 42只SD大鼠随机分成7组,其中1组作为正常对照组喂饲正常饲料、饮用正常自来水;另外6组喂饲缺铜饲料、饮用去离子水,同时分别给予不同剂量葡萄糖酸铜,依次为缺铜模型对照组(0ADI,0 mg/ml)、1/625ADI(0.000128 mg/ml)、1/125ADI(0.000 64 mg/ml)、1/25ADI(0.003 2 mg/ml)、1/5ADI(0.016 mg/ml)及ADI(0.8 mg/ml)组,30d后测定其血中铜蓝蛋白(CP)活力作为判断机体铜缺乏标准,测定其血中铜、锌含量及血中总胆同醇(TC)、甘油三脂(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL),进行相关性研究.结果 铜摄人量不足时,各组大鼠血中铜蓝蛋白活力低于正常水平(P＜0.05),说明机体处于铜缺乏状态.此时,除1/25ADI组外,其他各组血铜值变化不明显而各组血中铜/锌比值(Cu/Zn值)均高于正常水平(P＜0.05),且缺铜模型组、1/625ADI组代、HDL、LDL水平均明显低于正常对照组(P＜0.05).在1/25 ADI组血铜及Cu/Zn值均出现异常升高(P＜0.05),而TC、HDL、LDL值异常降低(P＜0.05).缺铜模型组大鼠血中TG水平高于正常水平(P＜0.05),当有少量铜摄入后,1/625ADI、1/125ADI及1/25ADI各组TG水平明显低于正常对照组(P＜0.05),且随着铜摄入量的升高而升高.结论 铜摄人量低于日常摄入量时,机体处于铜缺乏状态,此时血中Cu/Zn值反应比血铜更为敏感.缺铜会导致血中TC、TG、HDL、LDL低于正常水平,而在1/25ADI时TC、HDL、LDL异常降低,0mg/ml铜摄入量组TG异常升高.     

高锌摄人对机体血脂 脂蛋白及载脂蛋白代谢的影响

目的：观察健康人高锌摄入对血脂、脂蛋白及栽脂蛋白代谢的影响，为合理用锌提供依据。方法：选择长清县五峰乡当地居民38例，服葡萄糖酸锌(0.2g，含锌50mg／d)，每次专人送药，连服8周。在服药前、服药后4、8周3次查体，描记心电图，测定血压。3次查体同时取静脉血，测定胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL—C)、高密度脂蛋白胆固醇(HDL—C)、载脂蛋白AI(apoA1)、载脂蛋白B100(apoB100))含量，观察动态变化。结果：服锌4周时TC升高，8周持续升高，TG4周时升高，8周时处稳定状态，8周时LDL—C升高，4周时apoB100升高，8周时apoA1降低，apoA1／apoB100值变小。结论：健康人摄入锌50mg／d时，引起脂类代谢紊乱，临床应谨慎使用锌制剂。     

2型糖尿病并冠心病患者胰岛素抵抗和血脂的临床观察

目的　探讨 2型DM合并CHD患者胰岛素抵抗 (IR)及血脂变化的临床意义。方法　 5 5例 2型DM合并CHD患者、5 0例 2型DM非CHD患者及 45例对照者 ,空腹采血检测其血清Glu、Ins、TC、TG、HDL -C和LDL -C等 ,并将这些指标的变化进行组间对比分析。结果　与对照组比较 ,2型DM并CHD组仅HDL -C水平无差异 ,其它指标均有非常显著性差异 (均为P <0 0 1) ;2型DM非CHD组HDL -C和LDL -C两者无差异 ,其它指标亦有非常显著性差异 (均为P <0 0 1) ;而 2型DM并CHD组与非CHD组比较 ,除TC外 ,其它指标均有非常显著性差异 (P <0 0 5或P <0 0 1) ;各项指标高于参考值上限的检出率中Glu、TC和LDL -C无明显差异 (P >0 0 5 ) ,TG有明显差异 (P <0 0 5 ) ,Ins和HDL -C有非常显著性差异 (P <0 0 1)。结论　 2型DM并CHD患者的发病可能与IR和血脂代谢紊乱等因素共同作用有关。     

纳豆激酶粗提液对小鼠实验性高脂血症的降脂作用

目的　探讨纳豆激酶粗提液对小鼠实验性高脂血症的降血脂作用。方法　实验分别用花生油和猪油造成高脂模型对照组(PE -HFD ,LA -HFD) ,每种模型分组分别添加4 %纳豆激酶粗提液和8%纳豆激酶粗提液。饲养观察2 8天后,用显色-比色法测定血清总胆固醇(TC)和血清高密度脂蛋白胆固醇(HDL -C) ,并计算出HDL -C/TC值和动脉硬化指数(AI)。结果　实验中的自制高脂饲料可以成功地诱导小鼠产生高胆固醇血症及动脉硬化(P <0 . 0 1 )。添加4 %纳豆激酶粗提液组及添加8%纳豆激酶粗提液组的TC和AI均极显著低于HFD组(P <0 . 0 1 ) ,HDL -C/TC均极显著高于HFD组(P <0 . 0 1 ) ;添加4 %纳豆激酶粗提液组的HDL -C显著高于HFD组(P <0 . 0 5 ) ;添加8%纳豆激酶粗提液组的HDL -C极显著高于HFD组(P <0 . 0 1 )。且添加8%纳豆激酶粗提液的对上述四种指标的影响效果都优于4 %纳豆激酶粗提液组(P <0 . 0 1 )。结论　纳豆激酶粗提液具有良好的预防和降低血脂作用。     

老年冠心病患者餐后血脂变化及其对血小板活性的影响

目的　探讨老年冠心病 (CHD)患者餐后血脂代谢特点以及对血小板活性的影响。方法　对 48例老年冠心病 (CHD)患者 (冠心病组 )和 3 0例老年健康自愿者 (对照组 )均禁食 12h后 ,分别接受脂肪餐负荷试验 ,于空腹及餐后 4h测定血清甘油三酯(TG)、总胆固醇 (CH)、高密度脂蛋白 (HDL -C)、低密度脂蛋白 (LDL -C)水平 ,并同时于空腹状态及餐后 4h采用生物活性法测定血小板衍生生长因子 (PDGF)、用酶联免疫标测法 (ELISA)检测血管性假血友病因子 (vWF)浓度。结果　冠心病组餐前及餐后 4hTG(P<0 0 1)、CH(P <0 0 5 )、LDL -C(P <0 0 1)、PDGF(P <0 0 1)、vWF(P <0 0 1)值均显著高于对照组 ,HDL -C(P <0 0 1)显著低于对照组。餐后 4h与餐前比较 ,TG、CH、LDL -C值明显上升 ,而HDL -C下降幅度无明显差异。冠心病组PDGF、vWF值在餐后 4h上升幅度存在显著性差异 (P <0 0 5 )。结论　老年冠心病人存在血脂代谢异常。餐后血脂更能反映老年人的代谢水平。餐后血脂代谢异常对血小板活化起促进作用 ,血小板反复活化可能参与了冠状动脉硬化早期病变     

2型糖尿病患者血清超敏C-反应蛋白的表达特性

目的　探讨 2型糖尿病患者血清超敏C -反应蛋白 (hs-CRP)的表达特性。方法　利用胶乳增强免疫比浊法定量检测健康对照组 10 0例、2型糖尿病患者 130例 (其中糖尿病无大血管并发症 75例 ,有大血管并发症 5 5例 )的血清hs -CRP和脂蛋白 (a) (Lp(a) ) ,酶法检测总胆固醇TC ,高、低密度脂蛋白胆固醇(HDL -C、LDL -C)。结果　①hs-CRP在健康对照组为 (0 . 78± 0 .5 5 )mg/L ,糖尿病无大血管病变组为 (3 .0 5±1 .2 1)mg/L ,糖尿病并发大血管病变组为 (4 6 5± 1 .0 3)mg/L ,与健康对照组比较不论有无大血管并发症差异均有显著性差异 (P <0. 0 0 1)。②糖尿病并发大血管病变组总胆固醇、LDL -C和Lp(a)比健康对照组均明显增高 (P <0 .0 0 1) ;其总胆固醇、LDL -C、Lp(a)和hs-CRP浓度与糖尿病无大血管病变组之间的差异也有显著意义 (P <0 . 0 1)。结论　hs -CRP对糖尿病患者发生大血管意外预测和预防具有重要意义。     

葡萄糖酸锌对锌铜代谢及氧化功能的试验研究

目的 :研究锌对锌铜代谢及抗氧化功能的影响。方法 :选择新西兰大白兔 2 0只 ,以饮水中加葡萄糖酸锌 (锌含量 1 5.3mmol/ L)喂养 ,加锌前、加锌后 3月及 6月 3次取静脉血 ,6月时取血后处死动物 ,取心、肝、肾及肌肉 ,动态观察血液及组织锌铜含量、超氧化物歧化酶活性( SOD)及脂质过氧化物水平变化。结果 :加锌后实验组大白兔血清锌含量随锌摄入量的增加持续升高 ( P<0 .0 1 ) ,组织锌也显著升高 ( P<0 .0 1 ) ,血清为 5.87倍、肾 4.93倍、肝 2 .0 3倍、心肌 1 .7倍、肌肉 1 .57倍。SOD活性持续降低 ( P<0 .0 1 ) ,LPO持续升高 ( P<0 .0 1 ) ,SOD/ LPO比值变小 ( P<0 .0 1 ) ,铜无变化 ( P>0 .0 5)。结论 :高锌摄入能使内环境锌含量升高 ,抑制抗氧化功能。     

单纯性肥胖儿童血清IGF-Ⅰ和血脂变化及相关性研究

目的:探讨单纯性肥胖儿童(SOC)血清胰岛素样生长因子(IGF-Ⅰ)和血脂水平及两者相关性。方法:单纯性肥胖儿童和正常对照组儿童各50例,应用常规生化检验方法检测其总胆固醇(TC)、甘油三脂(TG)、低密度脂蛋白胆固醇(LDL-C)及高密度脂蛋白胆固醇(HDL-C)等,用ELISA法测定IGF-Ⅰ水平。结果:与健康儿童相比,单纯性肥胖儿童血清IGF-Ⅰ、TG、TG、LDL-C及ApoB均明显高于对照组(P<0.05),而HDL-C、ApoAI均明显低于对照组(P<0.05)。血清IGF-Ⅰ的浓度与血脂TC、TG、LDL-C及ApoB呈负相关关系(P<0.01),与HDL-C、ApoAI呈正相关关系(P<0.01)。结论:血清IGF-Ⅰ浓度的改变与单纯性肥胖儿童血脂代谢紊乱存在显著相关性。     

妊娠高血压综合征与血脂、载脂蛋白及脂蛋白(a)的关系

目的探讨妊娠高血压综合征(简称妊高征)患者血脂代谢水平的变化及其临床意义。方法测定正常孕妇及妊高征患者的血清甘油三脂(TG)、总胆固醇(TC)、载脂蛋白AI(ApoAI)、载脂蛋白B(ApoB)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)及脂蛋白(a)水平。结果妊高征组血清TG(P<0.01)、ApoB(P<0.01)、LDL-C(P<0.05)及LP(a)(P<0.05)较正常孕妇显著升高,ApoAI、HDL-C则显著降低(P<0.05),TC在两组间差别无显著性意义(P>0.05)。在轻、中、重妊高征患者之间,ApoAI(P<0.05)及HDL-C(P<0.01)依次降低,组间比较有显著性意义,LDL-C(P<0.01)及LP(a)(P<0.01)依次升高,组间有极显著性差异。TG、TC和ApoB组间比较,差别无显著性意义。结论血脂代谢异常可能在妊娠高血压疾病的发病中起一定作用。     

两种长效避孕针对脂质及脂蛋白的影响

64例育龄妇女分别每月注射NET—EN/EV(31例)和DMPA/EC(33例)避孕针,于注射前、注射第3、4、9月及停针后第3个月分别测定空腹血清TC、TG、HDL-C、LDL—C、ApoAⅠ、AⅡ和B水平。结果显示,注射NET—EN/EV避孕针后,各项参数指标均有不同程度的下降(P<0.05～0.001),停止注射后TC、TG、HDL—C、LDL—C、ApoAI水平均恢复到注射前水平,ApoAⅡ和B呈逐渐恢复趋势。注射DMPA/EC避孕针后,TC、TG、LDL-C无明显变化,HDL—C、ApoAI、AⅡ和B于注射后呈下降趋势,与注射前相比差异显著,但停止注射后,这些参数指标基本恢复。在整个观察期间,两种避孕针的HDL—C/TC和ApoAI/ApoB比值未见明显变化。本研究提示,两种长效避孕针安全、有效、使用方便,对心血管疾病的发病率及危险性无明显不良影响,值得推广和使用。     

绝经后妇女冠心病患者性激素水平与脂质代谢的临床观察

目的:观察绝经后妇女冠心病(CHD)患者血清性激素水平与脂质代谢关系。方法:58例绝经后妇女CHD患者作为观察组,58例绝经后健康妇女作为对照组,测定两组性激素相关指标(血清雌二醇(E2)、孕酮(P)、睾酮(T)、催乳素(PRL)、黄体生成素(LH)、促卵泡激素(FSH)、E/T)及脂质代谢相关指标(总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、载脂蛋白B(ApoB)、HDL-C/LDL-C、HDL-C/TC)水平,并进行相关性分析。结果:观察组患者E2、HDL-C、E/T、HDL-C/LDL-C、HDL-C/TC水平明显低于对照组,TC、TG、LDL-C、ApoB水平均明显高于对照组,差异有统计学意义(P<0.01或P<0.05);经多元回归分析观察组患者E2、E/T与TC、TG、LDL-C、ApoB水平呈负相关。结论:绝经后妇女CHD患者血脂异常与性激素下降密切相关,且共同参与CHD发生、发展。     

川崎病儿童载脂蛋白和脂蛋白及血脂水平变化的临床研究

[目的]研究川崎病(Kawasaki disease ,KD)患儿血清脂类(载脂蛋白、脂蛋白、血脂)在病程各临床分期中的变化情况. [方法]120例川崎病患儿在疾病的不同临床分期及120名对照组儿童均进行血清脂类测定. [结果]川崎病组血清脂类无年龄与性别差异,病程中TG、ApoB100、ApoE比对照组高(P＜0.01),TC、HDL-C、ApoAI比对照组低(P＜0.01),LDL-C、Lpa与正常儿童相比差异无显著性(P均＞0.05).急性期明显下降的项目有:TC、HDL-C、ApoAI、Lpa;除了ApoE,其余项目亚急性期均比急性期高.TC、HDL-C在亚急性期已接近正常水平,TG、LDL-C、Lpa、ApoAI、ApoB100在恢复期时基本达到正常水平.ApoE在KD病程中各期差异无显著性(P＞0.05),但水平始终高于对照组(P＜0.01). [结论]KD患儿血清脂类除ApoE外,在临床各期均有不同程度变化,但基本上是一过性的,到恢复期时基本上接近正常水平.KD患儿在疾病过程中存在的血脂代谢紊乱对其可能有长远的影响,临床应重视对血脂结果的分析,及早干预.     

Interactions of the LIPG 584C > T polymorphism and alcohol consumption on serum lipid levels

Both endothelial lipase gene (LIPG) 584C > T (rs2000813) polymorphism and alcohol consumption modulate serum lipid levels. But their interactions on serum lipid profiles are not well known. The present study was undertaken to detect the interactions of LIPG 584C > T polymorphism and alcohol consumption on serum lipid levels. Genotyping of the LIPG 584C > T was performed in 763 unrelated nondrinkers and 520 drinkers aged 15–85 years. The levels of serum total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), apolipoprotein (Apo) AI, and the ratio of ApoAI to ApoB were higher in drinkers than in nondrinkers (P < .01 for all). There were no significant differences in the genotypic and allelic frequencies between nondrinkers and drinkers. The levels of TC, HDL-C, and ApoAI in nondrinkers were different among the three genotypes (P < .05–.01), the subjects with CT genotype had higher TC, HDL-C, and ApoAI levels than the subjects with CC genotype. The levels of HDL-C and ApoAI in drinkers were different among the three genotypes (P < .001 and P < .05; respectively), the individuals with TT genotype had higher HDL-C and ApoAI levels than the individuals with CT and CC genotypes. The interactions between LIPG 584C > T genotypes and alcohol consumption on serum HDL-C (P < .01) and ApoAI levels (P < .05) were also detected by using a factorial regression analysis after controlling for potential confounders. The levels of TC in nondrinkers were correlated with LIPG 584C > T alleles (P < .05), whereas the levels of TG and HDL-C were associated with LIPG 584C > T alleles (P < .05) and genotypes (P < .05), respectively. These results suggest that the subjects with TT genotype benefit more from alcohol consumption than the subjects with CT and CC genotypes in increasing serum HDL-C and ApoAI levels.     

Modulation of paraoxonases during infectious diseases and its potential impact on atherosclerosis

Background

Niemann-pick C1-like 1 (NPC1L1) is a key protein for intestinal cholesterol transportation. Common single nucleotide polymorphisms (SNPs) in the NPC1L1 gene have been associated with cholesterol absorption and serum lipid levels. The present study was undertaken to explore the possible association of NPC1L1 rs2072183 1735?C?>?G SNP and several environmental factors with serum lipid levels in the Mulao and Han populations.

Methods

Genotyping of the rs2072183 SNP was performed in 688 subjects of Mulao and 738 participants of Han Chinese. The interactions between NPC1L1 1735?C?>?G polymorphism and several environmental factors on serum lipid phenotypes were tested using the factorial design covariance analysis after controlling for potential confounders.

Results

The frequency of G allele was lower in Mulao than in Han (29.72% vs. 37.26%, P?<?0.001). The frequency of CC, CG and GG genotypes was 49.85%, 40.84% and 9.31% in Mulao, and 39.30%, 46.88% and 13.82% in Han (P?<?0.001); respectively. The levels of low-density lipoprotein cholesterol (LDL-C), apolipoprotein (Apo) B and the ratio of ApoAI/ApoB in Han but not in Mulao were different among the three genotypes (P?<?0.05 for all), the subjects with GG and CG genotypes had higher LDL-C, ApoB levels and lower ApoAI/ApoB ratio than the subjects with CC genotype. Subgroup analysis showed that the G allele carriers in Han had higher total cholesterol (TC), LDL-C and ApoB levels in males (P?<?0.05) and lower ApoAI/ApoB ratio in both sexes (P?<?0.05) than the G allele noncarriers. The G allele carriers in Mulao had higher TC and LDL-C levels in males (P?<?0.05) and lower high-density lipoprotein cholesterol (HDL-C) levels in both sexes (P?<?0.05) than the G allele noncarriers. Serum TC, LDL-C, ApoB levels and ApoAI/ApoB ratio were correlated with genotypes in Han males (P?<?0.05) but not in females. Serum lipid parameters were also correlated with several environmental factors. The genotypes of rs2072183 SNP were interacted with gender or cigarette smoking to influence serum TC and HDL-C levels in Mulao, whereas the genotypes of rs2072183 SNP were interacted with several environmental factors to influence all seven lipid traits in Han (P?<?0.05-0.01).

Conclusions

The present study suggests that the rs2072183 SNP in NPC1L1 gene and its association with serum lipid profiles are different between the Mulao and Han populations. The difference in serum lipid profiles between the two ethnic groups might partly result from different rs2072183 SNP or NPC1L1 gene-environmental interactions.     

重症COPD人群脂代谢紊乱与CRP水平变化的临床分析

目的:观察重症慢性阻塞性肺疾病(COPD)人群脂代谢紊乱与CRP水平的变化,探讨其对预后的影响。方法:97例健康体检者为对照组,85例重症COPD人群为观察组,采用全自动生化分析仪测定甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、载脂蛋白A1(ApoA1)、载脂蛋白B(ApoB)、ApoA1/ApoB、HDL-C/LDL-C、HDL-C/TC、脂蛋白a(Lipa)浓度;采用免疫散射比浊法测定CRP水平。结果:观察组人群TG、CRP水平明显高于对照组;HDL-C、ApoA1、Lipa浓度明显低于对照组,差别均有统计学意义;经多元回归分析重症COPD人群血清脂质代谢水平与CRP水平密切相关。结论:重症COPD人群脂代谢发生紊乱,血清TG、CRP水平明显升高,HDL-C、ApoA1、Lipa浓度明显降低,其脂代谢水平与CRP密切相关;早期诊断及时治疗与预后密切相关,诊治过程中应控制脂代谢紊乱与CRP变化并加强护理,提高治疗效果,改善预后,防止或延缓心血管疾病等并发症发生。     

Association of rs5888 SNP in the scavenger receptor class B type 1 gene and serum lipid levels

Background

Bai Ku Yao is a special subgroup of the Yao minority in China. The present study was undertaken to detect the association of rs5888 single nucleotide polymorphism (SNP) in the scavenger receptor class B type 1 (SCARB1) gene and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.

Methods

A total of 598 subjects of Bai Ku Yao and 585 subjects of Han Chinese were randomly selected from our stratified randomized cluster samples. Genotypes of the SCARB1 rs5888 SNP were determined by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis, and then confirmed by direct sequencing.

Results

The levels of total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), apolipoprotein (Apo) AI were lower but ApoB was higher in Bai Ku Yao than in Han (P?<?0.05-0.001). The frequencies of C and T alleles were 78.3% and 21.7% in Bai Ku Yao, and 73.7% and 26.3% in Han (P?<?0.01); respectively. The frequencies of CC, CT and TT genotypes were 60.0%, 36.6% and 3.4% in Bai Ku Yao, and 54.2%, 39.0% and 6.8% in Han (P?<?0.01); respectively. The subjects with TT genotype in both ethnic groups had lower HDL-C and ApoAI levels than the subjects with CC or CT genotype (P?<?0.05 for all). Subgroup analyses showed that the subjects with TT genotype in Bai Ku Yao had lower HDL-C and ApoAI levels in males than the subjects with CC or CT genotype (P?<?0.05 for all), and the T allele carriers had higher TC, LDL-C and ApoB levels in females than the T allele noncarriers (P?<?0.05 for all). The participants with TT genotype in Han also had a lower tendency of HDL-C and ApoAI levels in males than the participants with CC or CT genotype, but the difference did not reach statistically significant (P?=?0.063 and P?=?0.086; respectively). The association of serum HDL-C and ApoAI levels and genotypes was confirmed by the multiple linear regression analysis in both ethnic groups. Serum lipid parameters were also correlated with several environmental factors.

Conclusions

The differences in serum lipid levels between the two ethnic groups might partially attribute to the differences in the SCARB1 rs5888 SNP and several environmental factors.     

糖尿病血糖 血脂及钾 钠 氯 镁元素的相关性分析

目的:探讨两组血糖、血脂水平、血清K、Na、Cl、Mg之间的相关性。方法:以231例已确诊的糖尿病患者为糖尿病组,以121例非糖尿病患者为非糖尿病组比较分析各指标之间的相关性。结果:①两组TC、HDL—C、LDL—C水平存在差异(P<0.05)。两组间K、Na、Cl、Mg、TG、ApoA1、ApoB、Apo(a)差异无统计学意义(P>0.05);②空腹血糖与Na,HDL—C呈负相关(P<0.01);TC与TG、LDL—C呈正相关(P<0.01);TC与ApoB呈正相关(P<0.05);TG与ApoA1、ApoB呈正相关(P<0.05);LDL—C与Apo(a)呈正相关(P<0.05);ApoA1与ApoB呈正相关(P<0.01)。结论:①糖尿病组K、Na、Cl、Mg微量元素水平与血糖无相关性;②血糖与Na负相关,可能是高血糖渗透利尿导致Na丢失;③脂代谢紊乱与糖尿病联系密切,脂代谢可作为判断糖尿病患者血糖控制好坏的标准;④脂类中各成分水平有相关性,ApoA1可作为动脉粥样硬化发生的间接指标。