采用TLC和HPLC法检查炎可宁片中的土大黄苷

目的：建立TLC结合HPLC法检查炎可宁中的土大黄苷的检测方法;方法：采用硅胶G板,乙酸乙酯-丁酮-甲酸-水（10：7：1：1）为展开剂,在365nm下检视;采用Lichrospher C18（4．6mm×250mm,5μm）,乙腈-甲醇-水（7：30：63）为流动相,检测波长为320nm。结果：炎可宁片非法掺入的土大黄的指标性成分土大黄苷在365nm下显持久的蓝紫色荧光斑点。通过高效液相色谱法进一步验证。对25批市售的炎可宁片进行筛查,检出土大黄苷的炎可宁片有14批。结论：TLC结合HPLC法简便,快速,专属性强,能有效地检查炎可宁片是否掺入土大黄。     

牛黄消炎片、妇乐颗粒中掺入土大黄的鉴别方法研究

目的建立TLC、HPLC法鉴别牛黄消炎片、妇乐颗粒中是否掺入土大黄。方法采用TLC法,硅胶G板,醋酸乙酯-丁酮-甲酸-水(10∶7∶1∶1)为展开剂,在365 nm下检视;采用HPLC法,Lichrospher C18(250 mm×4.6 mm,5μm),乙腈-甲醇-水(7∶30∶63)为流动相,检测波长为320 nm。结果土大黄苷在365 nm下显持久的蓝紫色荧光斑点,高效液相色谱法中呈现色谱峰。结论鉴别法简便、快速、专属性强,能有效地检查牛黄消炎片、妇乐颗粒是否掺入土大黄苷。     

复方陈香胃片中非法成分土大黄苷的检测

目的 建立定性检测复方陈香胃片中非法成分土大黄苷的方法.方法 采用TLC法、HPLC法对怀疑含有非法成分土大黄苷的复方陈香胃片进行分离分析,并采用HPLC - DAD法定性鉴别土大黄苷.结果 在复方陈香胃片中检测到含有非法成分土大黄苷.结论 所用方法专属性强、灵敏度高、操作简便,可作为检测非法成分土大黄苷的有效方法.     

采用HPLC-DAD和HPLC/MS法检查牛黄解毒片中的土大黄苷

目的:建立HPLC-DAD结合HPLC-MS法检查牛黄解毒片中的土大黄苷的检测方法.方法:以C18柱为色谱柱,以乙腈-水(25∶75)为流动相,采用二极管阵列检测器,检测波长为326nm.并采用HPLC/MS法对检测结果进行验证.结果:对18批市售的牛黄解毒片进行筛查,检出土大黄苷的牛黄解毒片有6批.结论:该方法简便,快速,专属性强,能有效地检查牛黄解毒片中是否掺入土大黄苷.     

复方龙胆碳酸氢钠片中非法成分土大黄苷定性筛查与定量测定方法的建立

目的:建立复方龙胆碳酸氢钠片组方药材大黄伪品中指标性成分土大黄苷的定性筛查与定量测定方法。方法:从药品流通领域抽样获取来自国内8家药品生产企业(编号:A～H)的45批复方龙胆碳酸氢钠片,采用薄层色谱法(TLC)初步定性鉴别样品中的土大黄苷;采用高效液相色谱法(HPLC)对土大黄苷进行含量测定;采用超高效液相色谱-串联质谱法(UPLC-MS/MS)进一步对检出的土大黄苷成分进行结构确证。结果:TLC法检测结果显示,来自D企业的10批样品在紫外光灯(365 nm波长)可见土大黄苷的亮蓝色荧光斑点。HPLC法方法学考察结果显示,土大黄苷质量浓度在0.884～88.4μg/m L范围内与峰面积呈良好的线性关系(r=0.999 9);进样量检出限、定量限分别为0.707 2、3.536 ng;精密度、重复性、稳定性试验RSD均小于1%;平均加样回收率为96.55%(RSD=0.53%,n=6)。D企业产10批样品中土大黄苷含量为0.732 4～2.890 8 mg/g。UPLC-MS/MS法检测结果显示,D企业产样品与土大黄苷对照品均有质荷比(m/z)419.0的准分子离子峰和m/z 257.1、241.2的碎片离子峰。结论:建立的TLC法定性初筛、HPLC法定量测定与UPLC-MS/MS法结构确证相结合的检测方法操作简便、灵敏、可靠,可用于复方龙胆碳酸氢钠片中土大黄苷的定性筛查和定量测定。45批次抽检样品中,有来自1个生产企业的10批样品中检出土大黄苷成分,提示该企业在生产复方龙胆碳酸氢钠片时存在以伪品大黄药材替代正品的现象。     

HPLC法鉴别大黄及部分含大黄中成药的真伪

目的:建立大黄及三黄片的真伪鉴别方法.方法:采用HPLC法测定其中的土大黄苷;色谱柱为Agilent analytical TC-C18(4.6 mm×250 mm,5μm),流动相为乙腈·水(25:75),检测波长为323nm.结果:正品大黄和以正品大黄投料所生产的三黄片中均未检出土大黄苷,而3种伪品大黄中均检出土大黄苷.结论:所建立的方法专属性强、灵敏度高、重现性好,能用于鉴别大黄及部分含大黄中成药的真伪.     

HPLC测定复方瓜子金颗粒中蒙花苷的含量

目的:采用HPLC法建立测定复方瓜子金颗粒中蒙花苷含量的方法。方法:色谱柱:SunFire C18柱(150 mm×4.6 mm,3.5μm),流动相:乙腈-水-冰醋酸(25∶73∶2),流速:1.0 ml.min-1,检测波长:334 nm。结果:蒙花苷在2.35～14.10μg.ml-1此浓度范围内线性关系良好,r=0.999 7,平均加样回收率为98.76%,RSD=0.38%。结论:该方法简便、可靠、准确,可用于复方瓜子金颗粒的含量测定。     

抗菌消炎胶囊中土大黄苷的薄层鉴别及HPLC鉴别方法研究

目的 建立抗菌消炎胶囊中土大黄苷的快速检验方法.方法 采用薄层色谱法快速检验样品中的土大黄苷,以乙酸乙酯-丁酮-水(10∶7∶1)为展开剂,在紫外光灯(365 nm)下观察斑点荧光,并用高效液相色谱法确证.结果 土大黄苷有非常清晰的蓝紫色荧光,正品制剂在相应位置上无斑点;土大黄苷液相色谱主峰在325 nm有最大吸收.结论 本法操作简便、快速、准确.     

高效液相色谱法测定气管炎片中黄芩苷含量

目的 测定气管炎片的黄芩苷含量.方法 采用高效液相色谱(HPLC),流动相为甲醇-水-磷酸(45550.2),色谱柱为DiamonsilTMC18柱(250mm ×4.6mm,5 μm),检测波长为315 nm.结果 黄芩苷进样量线性范围为0.404～2.424 μg(r=0.9997),平均回收率为100.47%,RSD为1.16%.结论 HPLC法可用于气管炙片的质量控制.     

高效液相色谱法测定清肺抑火片中黄芩苷的含量

目的:探讨清肺抑火片的含量测定方法.方法:采用高效液相色谱法(HPLC法)测定清肺抑火片黄芩中黄芩苷的含量.色谱柱为Elite 0DS C18柱(250 mm×4.0 mm,5μm),流动相为甲醇-水-磷酸(45:55:0.2),流速为1 mL/min,检测波长为315 nm.结果:回归方程为Y=85.915 4 2 701.674 3X,r=0.999 3,平均回收率为97.93%,RSD为1.76%(n=6).结论:HPLC法简便、快速、可靠,可用于清肺抑火片的质量控制.     

Degraded and undegraded carrageenans and experimental gastric and duodenal ulceration

The prevention of histamine-induced gastric and duodenal ulceration in the guinea-pig has been examined using a series of undegraded and degraded carrageenans. Undegraded carrageenans were active at lower doses than degraded carrageenans. The high viscosity of the undegraded carrageenans in solution prevented their use in larger doses. Degradation of carrageenan without serious loss of sulphate, gives a product which allows the dose to be increased to an extent that its effect more than offsets the slight loss in activity caused by the degradation. No single feature of carrageenan structure can be related to anti-ulcer activity although degradation, and hence reduction of molecular size, generally reduces activity. Sulphate contents over 30% have little apparent effect on activity; κ-carrageenans were not consistently different in anti-ulcer activity from Λ-carrageenans. This contrasts with the antipeptic activity of carrageenans where κ-carrageenans are less active than their Λ-counter-parts. As with antipeptic activity, the degree of anti-ulcer activity is probably determined by a combination of structural features which includes molecular size and polyanionic properties.     

Affective and Anxiety Disorders and Alcohol and Drug Dependence:

Depression and anxiety frequently coexist in patients with substance use disorders. This clinically-oriented article examiens the relationship between these conditions and emphasizes data showing that substances of abuse can cause signs and symptoms of both depression and anxiety. These substance-related syndromes appear to have a different course and prognosis than uncomplicated, independent anxiety and major depressive disorders, and clinicians should consider the role of alcohol and other drugs in all patients presenting with these complaints. The authors will also outline an approach for diagnosing and managing patients with the combination of a substance use and depressive or anxiety disorder.     

Alcohol and Substance Use and Abuse in Women and Children

No abstract available for this article.     

Glucosidase and fucosidase inhibitors and Advances in nucleosides and nucleotides.

The American Chemical Society Symposium "Glucosidase and fucosidase inhibitors" took place on 1 April 1998 and was organized by Professors Zbigniew J Witczak (UConn, School of Pharmacy, CT, USA), Kuniaki Tatsuta (Waseda University, Tokyo, Japan) and Waldemar Priebe, MD (Anderson Cancer Center, University of Texas, USA). Professor Witczak provided introductory remarks including the status of existing glucosidase inhibitors, and chaired the morning session, which consisted of six lectures. The symposium was well received, and was particularly attractive for those interested in networking, as attendance was about sixty. In addition, some participants and attendees presented posters on the subject during the regular poster session organized by the Division of Carbohydrate Chemistry.