膝骨关节炎滑液中可溶性晚期糖基化终末产物受体水平与其病变程度的相关性

背景:研究表明骨关节炎患者关节滑液内可溶性晚期糖基化终末产物受体水平可能与关节炎病变的严重程度存在负相关,但在中国报道较少。目的:观察膝关节骨关节炎患者关节滑液内可溶性晚期糖基化终末产物受体水平与其病变严重程度的关系。方法:共纳入46名膝关节骨关节炎患者及14名健康对照者,纳入的骨关节炎患者符合美国风湿病学会骨关节炎的临床诊断标准。采用Kellgren-Lawrence的标准对膝关节骨关节炎病变严重程度进行分级,使用人可溶性晚期糖基化终末产物受体水平酶联免疫吸附试剂盒在酶标仪下检测测关节滑液的可溶性晚期糖基化终末产物受体水平。结果与结论:膝关节骨关节炎患者关节滑液可溶性晚期糖基化终末产物受体水平较健康对照组显著降低(P<0.01),且与膝关节骨关节炎病变严重程度呈显著独立负相关(r=-0.587,P<0.01)。结果表明关节滑液可溶性晚期糖基化终末产物受体水平可能与膝关节骨关节炎病变的严重性和进展程度相关。     

联合检测糖基化终末产物受体、糖化血红蛋白与冠状动脉钙化程度的相关性

目的：通过联合检测糖代谢异常患者糖基化终末产物受体（可溶性晚期糖基化终末产物受体sRAGE、内源性分泌型晚期糖基化终末产物受体esRAGE、裂解型晚期糖基化终末产物受体cRAGE）、糖化血红蛋白（HbA1C）,探讨其与冠状动脉钙化程度的相关性。方法选取住院患者96例,分为正常对照组（n＝30）、糖耐量异常组（n＝31）、糖尿病组（n＝35）。检测各组患者的sRAGE、esRAGE、cRAGE、HbA1C 水平,评估其与冠状动脉钙化程度的相关性。结果3组患者的 sRAGE、esRAGE、cRAGE、HbA1C水平为正常对照组＜糖耐量异常组＜糖尿病组,并具有统计学差异（P＜0．05）,且伴随着它们的升高,冠状动脉钙化积分值也逐渐升高（P＜0．05）。结论 sRAGE、esRAGE、cRAGE、HbA1C水平与冠状动脉钙化积分呈正相关的关系。联合检测糖基化终末产物受体、糖化血红蛋白可以预测糖代谢异常患者发生冠状动脉疾病的可能性,并可评估其病情严重程度及预后。     

血浆可溶性晚期糖基化终末产物受体对急性呼吸窘迫综合征的诊断价值及预后意义

目的 探讨血浆可溶性晚期糖基化终末产物受体对急性呼吸窘迫综合征诊断的价值及预后意义.方法 本研究为前瞻性病例对照研究,将201 1年2月至2012年9月东南大学附属第二医院重症监护病房（ICU）收治的43例符合2012年急性呼吸窘迫综合征柏林定义及诊断标准患者,根据急性呼吸窘迫综合征柏林定义的患者氧合状态分为轻、中、重度三组,同时收集无肺损伤的住院患者20例作为对照组,根据28 d转归将急性呼吸窘迫综合征组分为生存组和死亡组两个亚组.入院24h内测定血常规、血气分析、血生化、C反应蛋白（CRP）,记录最高体温（T）,进行急性生理学与慢性健康状况评分系统Ⅱ（APACHEⅡ）评分、序贯器官衰竭估计（SOFA）评分;采用酶联免疫吸附法（ELISA）检测可溶性晚期糖基化终末产物受体水平.采用SPSS 17.0统计分析软件进行数据处理.计量资料采用方差或Mann-Whitney U检验,计数资料比较采用x2检验;绘制受试者工作特征曲线（ROC）.结果 急性呼吸窘迫综合征组患者血浆可溶性晚期糖基化终末产物受体浓度均显著高于无肺损伤组[377 （286.3 ～ 548.0）pg/ml]（P均＜0.05）,中、重度急性呼吸窘迫综合征组[分别为3188（1984～ 3042）,3273 （3170～3265） pg/ml]患者的可溶性晚期糖基化终末产物受体水平高于轻度急性呼吸窘迫综合征组[1707（1649～ 1870） pg/ml] （P ＜0.05）;死亡组可溶性晚期糖基化终末产物受体浓度[2996（2768～3319）pg/ml]明显高于生存组[2147（1965 ～ 2449） pg/ml],有统计学意义;可溶性晚期糖基化终末产物受体诊断急性呼吸窘迫综合征的ROC曲线下面积为0.912,以842 pg/ml为截断值,灵敏度为92.7％,特异度为87.8％,优于CRP.结论 血浆可溶性晚期糖基化终末产物受体水平对急性呼吸窘迫综合征诊断有一定价值,高浓度的可溶性晚期糖基化终末产物受体与急性呼吸窘迫综合征不良预后相关.     

不同年龄段骨关节炎患者关节滑液中基质蛋白多糖的变化

目的：通过观察不同年龄段骨关节炎患者滑液中关节软骨基质蛋白多糖的变化与正常人比较，探索建立早期骨关节炎滑液中生化指标判断标准。方法：选择2002—01／2003—04上海中医药大学附属曙光医院骨科门诊治疗的膝关节骨关节炎患者60例，平均年龄47岁。无明显骨赘形成和关节间隙狭窄影像学表现，患者知情并同意。按年龄分为30-39岁组，平均年龄35．1岁；40～49岁组，平均年龄44．9岁；≥50岁组，平均年龄65．4岁。选择同期无关节疾患的正常者10例为正常对照组，参与者均知情同意，平均年龄38．7岁。收集患者和正常人的关节滑液2-5mL，应用酶联免疫吸附测定法检测滑液样本中蛋白多糖的水平。结果：纳入患者60例，正常者10人，均进入结果分析。应用酶联免疫吸附测定法检测不同年龄段骨关节炎组与正常组关节滑液中蛋白多糖含量：正常对照组滑液中蛋白多糖水平为（29．8&;#177;8．4）μg／L，30～39岁组升高[（94．9&;#177;74．2）μg／L，40～49岁达到最高[（288．7&;#177;118．6）μg／L，P〈0．01]，≥50岁组明显降低[（178．6&;#177;126．1）μg／L]，但仍高于正常对照组和30-39岁组（P〈0．01）。结论：骨关节炎患者滑液中蛋白多糖含量明显高于正常对照组，提示骨关节炎患者关节滑液中蛋白多糖含量可以作为判断早期骨关节炎的生化指标。     

不同年龄段骨关节炎患者关节滑液中基质蛋白多糖的变化

目的:通过观察不同年龄段骨关节炎患者滑液中关节软骨基质蛋白多糖的变化与正常人比较,探索建立早期骨关节炎滑液中生化指标判断标准。方法:选择2002-01/2003-04上海中医药大学附属曙光医院骨科门诊治疗的膝关节骨关节炎患者60例,平均年龄47岁。无明显骨赘形成和关节间隙狭窄影像学表现,患者知情并同意。按年龄分为30～39岁组,平均年龄35.1岁;40～49岁组,平均年龄44.9岁;≥50岁组,平均年龄65.4岁。选择同期无关节疾患的正常者10例为正常对照组,参与者均知情同意,平均年龄38.7岁。收集患者和正常人的关节滑液2～5mL,应用酶联免疫吸附测定法检测滑液样本中蛋白多糖的水平。结果:纳入患者60例,正常者10人,均进入结果分析。应用酶联免疫吸附测定法检测不同年龄段骨关节炎组与正常组关节滑液中蛋白多糖含量:正常对照组滑液中蛋白多糖水平为(29.8±8.4)μg/L,30～39岁组升高[(94.9±74.2)μg/L,40～49岁达到最高[(288.7±118.6)μg/L,P<0.01],≥50岁组明显降低[(178.6±126.1)μg/L],但仍高于正常对照组和30～39岁组(P<0.01)。结论:骨关节炎患者滑液中蛋白多糖含量明显高于正常对照组,提示骨关节炎患者关节滑液中蛋白多糖含量可以作为判断早期骨关节炎的生化指标。     

P物质与白细胞介素1β在膝骨关节炎发病中的作用及其相关性

目的：研究显示．炎性细胞因子和神经肽与炎性相关疾病关系密切。实验通过检测骨关节炎患者膝关节滑液中P物质和白细胞介素1β的含量，探讨P物质、白细胞介素1β和膝骨关节炎发病以及病情严重程度之间的相互关系。方法：选择2006—03／09在中南大学湘雅医院门诊就诊和病房住院的膝关节骨关节炎患者50例，根据综合评分法，轻度15例，中度20例，重度15例：选取急性膝关节创伤患者6例为对照组。分别在就诊时或手术前抽取膝关节滑液至少1mL，采用酶联免疫吸附试验法检测膝关节液中P物质与白细胞介素1β的含量。结果：56例患者均进入结果分析。①骨关节炎组膝关节液中P物质和白细胞介素1β浓度高于对照组，差异有显著性意义（P〈0．05）。②滑液中自细胞介素1β浓度与骨关节炎严重程度呈正相关（r=0．79，P〈0．01），滑液中P物质与骨关节炎严重程度呈正相关（，r=0．76，P〈0．01），滑液中自细胞介素1β与P物质浓度呈正相关（r=0．83，P〈0．01）。结论：①P物质和白细胞介素1β与膝骨关节炎的发病和病情发展密切相关。②P物质和白细胞介素1β在骨关节炎的发病及病情发展中可能相互协同作用。     

透明质酸、转化生长因子β1在膝关节骨性关节炎发病中的作用探讨

目的 探讨透明质酸（HA）、转化生长因子β1（TGFβ1）在膝关节骨性关节炎（OA）发病中的作用。方法提取23例OA病变轻微患者（OA第一组），28例病变严重患者（OA第二组），6例对照者（盘状半月板组）的滑膜组织及关节液，采用免疫组织化学方法检测膝关节滑膜组织中HA、TGFβ1的阳性细胞率及通过夹心ELISA法测定膝关节滑液中HA、TGFβ1含量。结果本组57份膝关节滑膜组织及关节滑液标本中，无论是对照组还是OA组中均能测出HA、TGFβ1。OA病变轻微组膝关节滑膜组织及关节滑液标本中HA、TGFβ1含量与对照组比较无显著性差异（P〉0.05），OA病变严重组膝关节滑膜组织及关节滑液标本中HA、TGFβ1含量显著低于病变轻微组和对照组（P〈0．05）。结论HA、TGFβ1在膝关节骨性关节炎的发病中起重要作用。HA与TGFβ1减少程度与OA关节软骨损伤程度具有一定的相关性。     

2型糖尿病大鼠骨折愈合障碍与体内晚期糖基化终末产物的变化

背景：近年来,晚期糖基化终末产物在骨组织领域的作用日益受到重视,而糖代谢紊乱是引起晚期糖基化终末产物增加的主要原因之一。 目的：观察2型糖尿病大鼠体内晚期糖基化终末产物表达的变化,并探讨其与糖尿病骨折愈合障碍的关系。方法：30只SD大鼠随机均分为2组,实验组制备2型糖尿病模型,对照组正常饲养。糖尿病模型制备成功后,所有大鼠建立左胫骨骨折牵引成骨模型,胫骨延长0.3 mm/d,持续14 d。 结果与结论：牵引结束后,X 射线摄片显示实验组糖尿病模型大鼠骨折断端之间牵引骨痂形成较对照组明显减少;骨痂组织学检查表现为微骨柱排列紊乱,初始基质前沿浅染。ELISA 法检测实验组血清和双侧骨痂组织中晚期糖基化终末产物水平较对照组明显升高（P 〈0.01）,骨钙素明显降低（P 〈0.01）。提示2型糖尿病大鼠骨折牵引骨痂生成障碍,而骨组织中晚期糖基化终末产物水平增高可能是导致2型糖尿病骨折愈合障碍的原因。     

不同程度膝骨关节炎患者滑液中几种细胞因子水平变化

目的探讨关节滑液中白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)含量与膝骨关节炎(OA)病变程度之间的关系。方法提取31例不同程度膝骨关节炎患者(OA组),8例对照组(非OA组)的膝关节滑液,采用酶联免疫吸附法(ELISA法)测定IL-1β、IL-6;放射免疫法检测透明质酸(HA)的水平。结果膝OA组关节滑液中IL-1β、IL-6的水平显著高于对照组(P<0.01),而HA含量则显著低于对照组(P<0.01)。轻度患者滑液中IL-6及HA已分别显著增高及降低(P<0.05)。IL-1β、IL-6的水平均有随病情程度加剧而升高的趋势。相关分析的结果显示滑液中IL-1β水平与病情程度呈正相关(r=0.48,P<0.01)。结论IL-1β、IL-6在骨关节炎的发病及病情发展中起重要作用。但在体内环境下单个细胞因子不造成关节滑液中HA的减少。     

巨噬细胞移动抑制因子与膝骨关节炎的相关性研究

目的探讨MIF在膝骨关节炎中的作用及其与IL-1β的相关性。方法研究对象包括膝骨关节炎患者45例,正常人28例,应用ELISA测定MIF和IL-1β在关节滑液中的表达程度。结果试验组与对照组的滑液中MIF及IL-1β表达水平作单因素方差分析。MIF的KOA试验组与对照组比较有统计学意义(P<0.01),KOA患者的滑液中MIF的水平显著高于对照组。IL-1β的试验组与对照组比较有统计学意义(P<0.01),KOA患者的滑液中IL-1β的水平显著高于对照组。试验组中MIF与IL-1β水平作Spearman相关分析,相关系数为r=0.945(P<0.01),两者呈正相关。结论MIF可能是KOA发病过程中促进关节滑液炎症、介导软骨退变的重要因子。     

Soluble receptor for advanced glycation end products (sRAGE) in plasma and synovial fluid is inversely associated with disease severity of knee osteoarthritis

Objectives:The aim of this study was to measure soluble receptor for advanced glycation end products (sRAGE) in plasma and synovial fluid of knee osteoarthritis (OA) patients and to determine the correlation between sRAGE levels and disease severity.Design and methods:Thirty-six OA patients and 15 healthy controls were enrolled in this study. OA grading was performed using the Kellgren–Lawrence classification. sRAGE levels in plasma and synovial fluid were analyzed by enzyme-linked immunosorbent assay.Results:Plasma sRAGE levels were significantly lower in OA patients than in healthy controls (P = 0.01). sRAGE levels in plasma were remarkably higher with regard to paired synovial fluid (P = 0.001). Additionally, sRAGE concentrations in plasma and synovial fluid showed significant inverse correlation with disease severity (r = ?0.65, P < 0.001 and r = ?0.55, P = 0.001, respectively). Further analysis showed that there was a strong positive correlation between plasma and synovial sRAGE concentration (r = 0.81, P < 0.001).Conclusions:sRAGE levels were significantly lower in OA patients compared with controls, and sRAGE levels in plasma and synovial fluid also decreased significantly as the disease severity increased. Accordingly, sRAGE levels could be used as a biochemical marker for assessing the severity and progression of knee OA.     

Soluble lectin-like oxidized low-density lipoprotein receptor-1 levels in synovial fluid are correlated with disease severity of knee osteoarthritis

Objectives

This study was aimed to measure soluble lectin-like oxidized low-density lipoprotein receptor-1 (sLOX-1) levels in serum and synovial fluid (SF) of knee osteoarthritis (OA) patients and to investigate the correlation between sLOX-1 levels and the disease severity.

Design and methods

Two hundred and sixteen OA patients and 83 healthy controls were enrolled in this study. All OA patients were scored for Kellgren–Lawrence (KL) grade (0–4). The sLOX-1 levels were measured by enzyme-linked immunosorbent assay (ELISA).

Results

In OA patients, the mean sLOX-1 levels in SF were markedly lower than those in paired serum samples (P < 0.01). The SF sLOX-1 levels increased with higher KL grade (P < 0.01) and were significantly correlated with disease severity (r = 0.324, P < 0.01). SF sLOX-1 level was the independent factor for predicting the disease severity of OA (β: 0.281, 95%CI: 0.145–0.396).

Conclusions

SF sLOX-1 levels were independently and positively associated with disease severity in knee OA.     

人膝骨性关节炎滑膜中表达的骨桥蛋白

背景：目前研究显示骨桥蛋白与骨性关节炎关节软骨退变密切相关,但骨桥蛋白与骨性关节炎滑膜病变是否相关,仍少见报道。目的：研究骨桥蛋白在原发性骨性关节炎发生与发展过程中的作用。方法：选取膝骨性关节炎患者关节滑膜标本与下肢外伤患者作对照,根据综合评分法进行分组,采用免疫组化方法检测关节滑膜中骨桥蛋白水平,比较不同程度骨性关节炎组膝关节滑膜中骨桥蛋白的差异,同时比较标本滑膜衬里层和衬里下层骨桥蛋白的水平。结果与结论：骨性关节炎组织中骨桥蛋白阳性呈黄色、棕黄色、棕褐色表达,病变程度越重,颜色表达越深。骨性关节炎组滑膜中骨桥蛋白明显高于对照组,差异有显著性意义（P〈0．05）,且随着骨性关节炎病情加重,骨桥蛋白的表达逐渐增多,呈正相关（p=0．663,P〈0．01）。但滑膜衬里层和衬里下层骨桥蛋白的水平差异无显著性意义（P〉0．05）。说明骨桥蛋白可能与骨性关节炎的发病有关。     

程序性细胞死亡因子5在活动性类风湿关节炎患者中的水平变化

目的：探究程序性细胞死亡因子5（programmed cell death factor 5,PDCD5）在类风湿关节炎（rheumatoid arthritis, RA）患者中的表达情况,并探讨PDCD5在RA发病中的可能作用。方法：采用酶联免疫吸附试验检测36例活动性RA患者和35例骨关节炎（osteoarthritis,OA）患者的血清和关节液中的PDCD5、白细胞介素6（interleukin 6,IL-6）水平,另以33名健康人的血清PDCD5水平作为对照（对照组）,比较3组间以上各指标水平的差异。同时再检测RA患者的C反应蛋白（C reaction protein, CRP）、红细胞沉降率（erythrocyte sedimentation rate, ESR）、类风湿因子（rheumatoid factor,RF）和环瓜氨酸肽（cyclic citrullinated peptide, CCP）,记录其肿胀关节数（swollen joint count,SJC）、疼痛关节数（tender joints count, TJC）,作为疾病活动指数参考指标。结果：RA组的血清PDCD5水平[（32.47±12.79） ng/mL]显著高于OA组[（12.79±9.84） ng/mL]及对照组[（18.40±18.97） ng/mL];RA组关节液中的PDCD5水平[（47.75±21.94） ng/mL]亦高于OA组[（19.33±11.25） ng/mL]。RA患者血清中的PDCD5水平与IL-6水平呈负相关（r=-0.431）,但在关节液中未观察到两者间具有相关性。Pearson分析显示,RA患者血清中的PDCD5水平与CRP、ESR、SJC、TJC呈负相关（R值分别为-0.523、-0.701、-0.845、-0.943）,但与CCP及RF水平间无统计学相关性。结论：活动性RA患者的血清及关节液中PDCD5的水平升高,而其表达失调可能与IL-6水平相关。     

Plasma and synovial fluid sclerostin are inversely associated with radiographic severity of knee osteoarthritis

ObjectiveThe purpose of this study was to analyze sclerostin in plasma and synovial fluid of knee osteoarthritis (OA) patients and to investigate the association between sclerostin levels and radiographic severity.Design and methodsA total of 190 subjects (95 knee OA patients and 95 healthy controls) were recruited in the present study. Sclerostin levels in plasma and synovial fluid were assessed using an enzyme-linked immunosorbent assay. OA grading was performed using the Kellgren–Lawrence classification.ResultsPlasma sclerostin levels were significantly lower in OA patients than in healthy controls (P = 0.004). Additionally, sclerostin levels in plasma were significantly higher with respect to paired synovial fluid (P < 0.001). Moreover, sclerostin levels in plasma and synovial fluid demonstrated a significant inverse correlation with the radiographic severity of knee OA (r = − 0.464, P < 0.001 and r = − 0.592, P < 0.001, respectively). Subsequent analysis revealed that there was a positive correlation between plasma and synovial sclerostin levels (r = 0.657, P < 0.001).ConclusionsSclerostin was significantly lower in OA plasma samples when compared with healthy controls. Plasma and synovial fluid sclerostin levels were inversely associated with the radiographic severity of knee OA. Therefore, sclerostin may be utilized as a biochemical marker for reflecting disease severity in primary knee OA.     

膝骨关节炎患者关节腔注射玻璃酸钠后血清及关节液中可溶性Fas蛋白的变化

背景：关节软骨细胞凋亡在骨关节炎的发病中起重要作用,Fas是肿瘤坏死因子受体家族成员,是细胞凋亡通路中的重要蛋白。目的：观察膝骨关节炎患者血清可溶性Fas蛋白水平的变化以及玻璃酸钠治疗对血清及关节液中可溶性Fas蛋白的影响。方法：选取64例膝骨关节炎患者和48名正常对照者,膝骨关节炎患者再随机分为常规治疗组和玻璃酸钠组。常规治疗组给予常规治疗,玻璃酸钠组在常规治疗的基础上,给予关节腔内注射玻璃酸钠2mL,1次/周,连续5周。检测正常组及膝骨关节炎患者治疗前后血清及关节液中可溶性Fas蛋白水平的变化。结果与结论：与对照组相比,膝骨关节炎患者血清可溶性Fas蛋白水平明显增高,差异有显著性意义。非条件logistic回归分析表明,升高的血清可溶性Fas蛋白水平是膝骨关节炎发病的独立危险因子。治疗5周后,玻璃酸钠组的血清及关节液中的可溶性Fas蛋白水平较治疗前明显下降,差异有显著性意义;而常规治疗组的血清及关节液中的可溶性Fas蛋白水平与治疗前相比无明显变化。提示玻璃酸钠具有降低膝骨关节炎患者血清及关节液中可溶性Fas蛋白水平的作用。     

类风湿关节炎患者自身抗体及关节损伤因子检出的临床意义

目的:检测类风湿关节炎(rheumatoid arthritis,RA)患者关节损伤因子及多种自身抗体,探讨其与RA发病间的关联性。方法:采用酶联免疫吸附试验(ELISA)法检测15例RA患者血清和滑膜液中关节损伤相关因子基质金属蛋白酶3(matrix metalloproteinases 3,MMP3)和基质金属蛋白酶组织抑制因子1(tissue inhibitor of metalloproteinase 1,TIMP-1)、骨破坏因子——核因子κB受体活化因子配基(receptor activator of NF-κB ligand,RANKL)和其保护性抑制剂骨保护素(osteoprotegerin,OPG)的表达。检测6例RA患者的sIL-6和sIL-6Rα、sgp130表达水平,同时设骨关节炎(osteoarthritis,OA)为对照组(6例)及正常对照组(8例)。分析22例RA患者临床常用的检测指标,包括类风湿因子、抗环瓜氨酸多肽抗体、葡萄糖-6-磷酸异构酶及23例RA患者抗胶原Ⅱ抗体的表达,并以OA患者为对照;针对用合成的EBV肽段和人HLA-DR4肽段检测和分析15例RA患者抗EBV肽段和抗HLA-DR4肽段抗体的阳性率。结果:RA患者关节腔滑膜液中MMP3和TIMP1表达异常,滑膜液MMP3/TIMP1比值显著高于相应的血清相应比值(P<0.001)。与OA组相比,RA患者血清及滑膜液中RANKL水平升高(P<0.05)。RA患者体内IL-6信号系统表达异常,其滑膜液中sIL-6Rα水平高于OA组(P<0.05),而天然拮抗剂sgp130的血清水平则显著低于OA组血清中(P     

Correlation of plasma and synovial fluid osteopontin with disease severity in knee osteoarthritis

Objectives

The purposes of this study were to examine osteopontin levels in both plasma and synovial fluid of patients with primary knee osteoarthritis (OA) and to investigate their relationship with severity of the disease.

Design and methods

Thirty-two patients aged 53-83 years with knee OA and 15 healthy controls were enrolled in this study. Anteroposterior knee radiographs were taken to determine the disease severity of the affected knee. The radiographic grading of OA in the knee was performed by using the Kellgren-Lawrence criteria. Osteopontin levels in the plasma and synovial fluid were measured using enzyme-linked immunosorbent assay.

Results

The mean plasma osteopontin concentration of the knee OA patients was significantly higher compared with that of healthy controls (168.8 ± 15.6 vs 67.2 ± 7.7 ng/mL, P < 0.0001). Osteopontin levels in synovial fluid were significantly higher with respect to paired plasma samples (272.1 ± 15.0 vs 168.8 ± 15.6 ng/mL, P < 0.001). In addition, plasma osteopontin levels showed a positive correlation with synovial fluid osteopontin levels (r = 0.373, P = 0.035). Subsequent analysis showed that plasma osteopontin levels significantly correlated with severity of disease (r = 0.592, P < 0.001). Furthermore, the synovial fluid levels of osteopontin also correlated with disease severity (r = 0.451, P = 0.01).

Conclusion

The data suggest that osteopontin in plasma and synovial fluid is related to progressive joint damage in knee OA. Osteopontin may serve as a biochemical marker for determining disease severity and could be predictive of prognosis with respect to the progression of knee OA.     

Association of MIF in serum and synovial fluid with severity of knee osteoarthritis

ObjectiveRecent evidences suggest that inflammation contributes to the development and progression of osteoarthritis (OA). This study aims to determine macrophage migration inhibitory factor (MIF) levels in serum and synovial fluid (SF) of patients with knee OA and to analyze the association of MIF levels with the radiographic severity of OA.Design and methods224 patients with knee OA and 186 healthy controls were enrolled in this study.ResultsHigher levels of serum MIF were found in knee OA patients compared with healthy controls. Knee OA patients with Kellgren and Lawrence (KL) grade 4 showed significantly elevated MIF levels in serum and SF compared with those with KL grade 2 and 3. MIF levels in serum and SF of knee OA patients were significantly related to disease severity evaluated by KL grading criteria.ConclusionMIF levels in serum and SF were closely related to the radiographic severity of OA.