豚鼠染色体的核型分析

本文报道了豚鼠体细胞染色体为2n=64。根据它的相对长度、臂比指数、着丝粒的位置,将64条染色体暂时分为四组,即A、B、C和D组。A组由X和1～7对中部着丝粒染色体组成。B组由8～17对亚端部着丝粒染色体组成。C组19～20对亚中部着丝粒染色体组成。D组由Y和21～31对端部着综粒染色体组成。第8对在所测量的染色体中是最大的。15、16、17对染色体在光镜下没有明显的形态差别,     

土耳其斯坦东毕吸虫结节变种的染色体核型

本文首次报道了土耳其斯坦东毕吸虫结节变种的染色体数目为2n=14,n=7。其核型分为 A、B、C 三组。A 组,1—2号染色体,1号为亚端着丝粒,2号为亚端着丝粒或亚中着丝粒。B 组,3—5号染色体,为亚端着丝粒或亚中着丝粒。C 组,6—7号染色体均为端着丝粒。A 组第2号染色体为性染色体。     

贵州道真产虎斑游蛇染色体组型

贵州道真产虎斑游蛇雌性染色体数为2n=38+ZW,分为大染色体和点状染色体两类。大染色体8对,其中第1,2,3,6对为中部着丝粒染色体,第4、7、8对为端部着丝粒染色体,第5对为性染色体,Z染色体为中部着丝粒染色体,W染色体为亚中部着丝粒染色体。比较了贵州道真、太原、辽宁及日本产虎斑游蛇染色体组型的异同。     

凹甲陆龟染色体组型的研究

目的：研究凹甲陆龟Testudo impressa(Guenther)的染色体组型级减数分型染色体。方法：以离体培养的外周血淋巴细胞、骨髓和精巢为材料，按常规的秋水仙素-低渗-空气干燥制片法制片和组型分析。结果：凹甲陆龟染色体数2n=52，包括A组8对大型中部和亚中部着丝粒染色体，B组7对大型亚端部和端粒部着丝染色体及C组11对微小染色体，精母细胞减数分裂终变期显示具有26个二价体，雌雄细胞之间未见异型染色体。结论：凹甲陆龟在N0.1、4-5、12-14号染色体的着丝粒位置上表现出种的特异性；在N0.14染色体长壁近着丝粒区有一对明显的次缢痕，凹甲陆龟在近缘染色体组型的演化中较四爪陆龟和缅甸陆龟原始。     

华支睾吸虫染色体组型的初步研究

本文以华支睾吸虫的精原细胞和卵原细胞为材料,分析了该虫的染色体组型。结果表明其染色体数目,2n=14,n=7。核型的组成是:大型染色体4个,小型染色体10个,可配成7对。根据染色体的大小、形态和着丝粒的位置可分成两组:第一组第1号染色体为大型亚中部或中部着丝粒染色体,第2号染色体为大型亚中部着丝粒染色体;第二组第3～7号染色体为五对小型亚端部着丝粒染色体。     

Vc-獭兔染色体核型的研究

目的确定Vc-獭免染色体核型与G-带核型。方法外周血淋巴细胞培养、常规染色体制片和染色体G-分带技术，对Vc-Ⅰ系、Vc-Ⅱ系獭免的染色体组型、G-带核型进行了研究。结果与结论Vc-Ⅰ系、Vc-Ⅱ系獭兔二倍体细胞染色体数为44，即2n=44，21对常染色体，1对性染色体(X、Y)。常染色体分为4组：A组，1～8号，为中着丝点染色体；B组，9～11号，为近中着丝点染色体；C组，12～17号，为近端着丝点染色体；D组，18～21号，为端着丝点染色体。X为近中着丝点染色体，Y为端着丝点染色体。Vc-獭兔的公兔染色体组型为44，XY。G-带核型表明：Vc-Ⅰ系、Ⅱ系免的染色体G-带型基本一致。     

孟氏裂头绦虫染色体组型和C分带的研究

本文以成虫的成熟节片,采用秋水仙素—低渗处理—空气干燥法技术.对孟氏裂头绦虫染色体数目、组型和C分带的作了分析研究。结果表明,孟氏裂头绦虫生殖细胞染色体数目为27(n=9、3n=27),可配成9组。组型是由12个中央着丝粒染色体,6个亚中着丝粒染色体,6个端着丝粒染色体和3个亚端着丝粒染色体组成。     

格氏血厉螨染色体组型及其C—带、G—带的研究

本文首次报道了厉螨科格氏血厉螨染色体组型及其C—带、G—带的研究。结果表明格氏血厉螨染色体组型为:n=5,2n=10。为单二倍体性决定系统,雄性体细胞具有5条染色体,雌性体细胞具有10条染色体。常规染色下未见明显的着丝粒。C—带研究显示单倍体组除最大的第一号染色体为亚中部着丝粒外,其余4条均为端部着丝粒染色体.G—带染色单倍体组中期分裂相约有24条深带。尚讨论了单二倍体系统的形成机制、染色体的多态性及分带研究等问题。     

短膜壳绦虫(H.nana)染色体组型分析

本文采用成熟节片短期培养,空气干燥法制片,首次分析了短膜壳绦虫的染色体组型。短膜壳绦虫染色体数目为2n=12,染色体组型由2对中部着丝粒染色体,1对亚中部着丝粒染色体,3对端部着丝粒染色体组成。     

曼氏迭宫绦虫染色体组型的初步研究

本文以成熟节片为材料,采用简易的细胞培养,秋水仙素处理,空气干燥法制片,结果表明曼氏迭宫绦虫的染色体数目为27(n=9,3n=27),可配成9组,核型的姐成是:中部着丝粒染色体12个(No5,6、7、8),亚中部着丝粒染色体9个(No1、3、4)和端部着丝粒染色体6个(No2、9)。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.