CHANGES OF UTERINE PROSTAGLANDIN IN RATS WITH COLD STAGNATION SYNDROME OF DYSMENORRHEAL AND INTERVENTION EFFECTS OF DIFFERENT QUANTITY OF ACUPUNCTURE STIMULATION

Objective: To observe the effects of different quantity of acupuncture stimulation on writhing response and uterine PGE2 and PGF2α content in rats with cold stagnation syndrome of dysmenorrheal.Methods: Forty female anestrous SD rats were randomly divided into 4 groups: saline group, model group, A stimuli group(thick needles, deep needling with manipulation), and B stimuli group(thin needles, shallow needling without manipulation).Except for the saline group, rats in the model group, A stimuli group and B stimuli group were administered with general freezing method combined with estradiol benzoate injection.After model preparation, acupuncture treatment was performed for 20 min.Writhing response, and the content of uterus tissue PGE2 and PGF2α in the rats were observed.Results: Compared with the saline group, the writhing latency of the model group was significantly shortened(P0.01), thewrithing number and writhing score of the model group were increased significantly(P0.01).Compared with the model group, the writhing latency was significantly longer(A stimuli group, P0.05), and the writhing number and writhing score decreased significantly(A stimuli group, P0.01; B stimuli group, P0.05); PGF2α decreased significantly(A stimuli group,P0.01; B stimuli group, P0.05), and PGE2 increased significantly(B stimuli group, P0.01).Conclusion: Acupuncture can obviously reduce the writhing response score and pain reaction, the possible mechanism of which is through regulating the uterine PGE2 and PGF2α levels.     

EXPERIMENTAL STUDY OF USING DIFFERENT AMOUNT OF STIMULATION WITH ACUPUNCTURE ON UTERINE CONTRACTION AND UTERUS MICROCIRCULATION IN A RAT MODEL OF DYSMENORRHEA WITH COLD COAGULATION SYNDROME

Objective: To observe the effects of using different amount of stimulation of acupuncture at Sanyinjiao(SP6) point on uterine contraction and uterus microcirculation in a rat model of dysmenorrhea with cold coagulation syndrome, and to explore the relationship between different amount of stimulation.Methods: Diestrus female rats were randomly divided into saline control group, model control group, A stimuli group(treated by thick needle, deep needling and hand-manipulating of needle) and B stimuli group(treated by thin needle, superficial needling and without hand-manipulating of needle), 8 rats in each group.The model control group was designed as dysmenorrhea model with cold coagulation syndrome.Behavior and uterine microcirculation were used as evaluation index.The A stimuli and B stimuli groups were treated based on the model, and the changes of the rat's uterine contraction and uterus microcirculation at different observation points during the 20-minute acupuncture treatment were observed.Results: In the uterine contraction test, compared with the model control group, the uterine contraction wave number(P0.01) and uterine activity(P0.05) of the A stimuli group were significantly reduced.In the uterus microcirculation test,compared with the model control group, the uterine microvascular and capillary diameter of the A stimuli group dilated obviously at every observation points, the difference was statistically significant(P0.01).For the uterus blood flow, compared with the model control group, the uterine microvascular blood flow of the A stimuli group was significantly improved during the acupuncture treatment(P0.01).Conclusion: Different amount of stimulation with acupuncture at SP6 can alleviated uterine tension and uterus microcirculation in dysmenorrheal rats with cold coagulation syndrome.The effects of using thick needle, deep needling with hand-manipulating of needle are better than using thin needle, superficial needling and without hand-manipulating of needle.We believed the formeramount of stimulation can achieve the acupuncture Deqi state more easily.     

EFFECTS OF DIFFERENT QUANTITY OF ACUPUNCTURE STIMULUS ON UTERINE MICROCIRCULATION IN DYSMENORRHEA RATS WITH COLD SYNDROME AT DIFFERENT TIMEPOINTS

Objective: To observe effects of different quantity of acupuncture stimulus on uterine microcirculation in dysmenorrhea rats with cold syndrome at different time-points, and to discuss the relationship between quantity of acupuncture stimulus and acupuncture effect.Methods: Female dioestrus rats were randomly divided into4 groups with 8 rats in each group.Rats of the model control group and the stimulus groups were frozen in a-25 ℃ freezer for 5 d, 4 h per day, while subcutaneous estradiol benzoate injection was given for 10 d, with intra-peritoneal injection of oxytocin,2 U/each, 1 h after the last injection.On the 10 th day, the stimulus groups were given different quantity of acupuncture stimulus on both sides of Sanyinjiao(SP6) for 20 min.Results: Compared to the saline control group, diameters of microvessel of the model control group at all time-points and diameters of capillary(except 40 and 60 min) were significantly decreased(P0.01), numbers of capillary were reduced significantly(P0.01), clarity of microvessel was worse with color getting darker, and blood flow was slower or stagnated(P0.01 or P0.05).Compared to the model control group, diameters of microvessel of the A stimuli group at all time-points, and diameters of capillary(only 5, 10, 20 and 30 min) were significantly increased(P0.01 or P0.05), clarity of microvessel(except 20 min) was better, color was(only 10, 20, 40 and 50 min) redder, and blood flow at all time-points was speeded up significantly(P0.01); diameters of microvessel of the B stimuli group were increased only at 30 min(P0.01), and clarity of microvessel(only 50 and 60 min) was better(P0.05).Compared to the B stimuli group, diameters of microvessel of the A stimuli group at all time-points and diameters of capillary(only 5, 10, 20 and 30 min) were significantly increased(P0.01 or P0.05), clarity of microvessel(only 30 and 40 min),color(only 5, 20 and 40 min) and blood flow(except 50 and 60 min) were significantly improved(P0.01 or P0.05).Conclusion: Acupuncture could improve uterine microcirculation.The effects of different quantity of acupuncture stimulus on uterine microcirculation were different, and the effect in A stimuli group was better.     

EFFECTS OF DIFFERENT ACUPUNCTURE STIMULI ON PAIN,UTERINE VASCULAR DIAMETER,AND TEMPERATURE OF ACUPOINT AREA IN RATS WITH COLD STAGNATION DYSMENORRHEA

Objective: To discover the relation between different acupuncture stimuli and the analgesic effect, uterine vascular diameter, and acupoint temperature in a rat model of primary dysmenorrhea.Methods: One hundred and four 3-month estrus female rats were randomly divided into saline group, cold stagnation dysmenorrhea model group(CSDM), A stimuli group, and B stimuli group(26 in each group).Different acupuncture stimuli(A and B) were applied to Sanyinjiao(SP6).Writhing response, diameters of uterine vessels, and temperature of SP6 and Xuehai(SP10) areas were observed.Results: Compared with the saline group, CSDM group had reduced latent period(P0.01), increased frequency and score of writhing(P0.01), and contracted uterine vessel(P0.01).Compared to the CSDM group, latent period was prolonged(P0.01), writhing frequency and score were reduced(P0.01) and uterine vessels were dilated(P0.05 or P0.01) in the A stimuli group.Writhing score of the B stimuli group was reduced(P0.05), with dilated uterine vessel(P0.01) compared to the CSDM group.Compared to the B stimuli group, the A stimuli group had prolonged latent period(P0.01), reduced frequency(P0.01) and score(P0.05) and dilated uterine vessels(P0.05 or P0.01).Compared with other groups, temperature of left SP6 in the A stimuli group reduced from the onset of the stimulation to the 5th minute(P0.05), while the temperature of one was increased from the 5th minute to the 10 th minute(P0.01).Temperature of right SP10 reduced from the onset of the stimulation to the 5th minute(P0.05 or P0.01), increased from the 5th minute to the 10 th minute(P0.05 or P0.01), and decreased again from the 10 th minute to the 20 th minute(P0.01).Conclusion: For the treatment of dysmenorrhea, the effect of thick needle with deep insertion and manipulation(A stimuli) is superior to thin needle with superficial insertion but no manipula-tion(B stimuli).Dilation of uterine vessels and improvement of microcirculation may be the underlying mechanism.Different acupuncture stimuli could trigger different patterns of temperature change in acupoints.     

RESEARCH OF ACUPUNCTURE EFFECTS ON THE FUNCTION OF SMOOTH MUSCLE CONTRACTION IN TREATING ASTHMATIC RATS

To explore the role of acupuncture in treating asthma disease, on the basis of airway smooth muscle cytoskeleton protein function,we observed the effects of acupuncture on smooth muscle contraction function of a rat model of asthma.In vivo, rats were exposed to ovalbumin sensitization to establish the rat model of chronic asthma and they were treated with manual acupuncture on Feishu(BL13), Shenshu(BL23) and Dingchuan(EXB1) acupoints(incidental and fundamental acupoints), once a day and 30 min each time for 3 months.We used pulmonary pathology HE staining and Sirius red staining to observe the airway structure and epithelial fibrosis for each group, and computer image analysis system for evaluation of airway remodeling.Force transduction device was applied to observe bronchial smooth muscle contraction curve.Contents of IL-6, IL-1β, TNF-α and NF-κB in bronchoalveolar lavage fluid were detected by ELISA.Simultaneously,the expression of α-SMA in lung and bronchial smooth muscle cells was observed by Western-blot and immunostaining methods.Furthermore, actin polymerization was detected as evidence of the immunoblot analysis.The airway remodeling structures were changed, such as incensing of inflammatory cell infiltration, losing of epithelial cells, epithelium of fibrosis, and increasing thickness of smooth muscle cell layer under the epithelium.Manual acupuncture alleviated the pathological changes.We found that smooth muscle cell contractility was improved significantly after acupuncture treatment.Contents of IL-6, IL-1β, TNF-α and NF-κB in bronchoalveolar lavage fluid were significantly decreased after acupuncture treatment.Acupuncture also reversed the increase of α-SMA protein expression in the lung and bronchus,and decreased the F/G actin ratio.In conclusion, acupuncture treatment can effectively regulate the airway smooth muscle contractility function of the asthmatic rats, and effectively restrain the airway remodeling and inflammatory reaction of asthma.     

针刺治疗小鼠实验性过敏性休克的研究

实验用JCR纯种小鼠,以5％的牛血清致小鼠过敏性休克,观察其针刺效应。 1.电针“人中、承浆”两穴,对于小鼠实验性过敏性休克具有良好的拮抗作用。 2.使用肾上腺素能受体拮抗剂分别阻断α、β受体,能够阻断电针的抗休克作用,说明针刺是通过某种途径兴奋肾上腺素受体而发挥作用的。 3.应用纳洛酮阻断内源性MLS受体或以其它方法抑制β-内啡肽的释放,则电针抗过敏性休克的作用增强。提示药物能弥补针效的不足,发挥针药协同作用是一个值得研究的途径。     

电针对大鼠跟腱断裂术后修复影响的实验研究

通过电针大鼠小腿部相关穴位,观察电针在大鼠跟腱断裂术后修复中对细胞因子TGFβ1和生物力学因素的影响,将48只大鼠先用手术方法造成跟腱断裂模型,再用Kessler法缝合并石膏绷带超踝膝固定1周后拆除,随即分为细胞因子实验组(A组)、细胞因子对照组(B组)、生物力学实验组(C组)、生物力学对照组(D组)各12只,A组和C组每天电针治疗1次,连续2个疗程(7天1个疗程),对照组B组和D组不予治疗。然后处死所有大鼠,A组和B组剪取跟腱断裂处组织块作H.E染色和免疫组化染色,观察组织学改变(纤维母细胞数)和TGFβ1变化(目标灰度值),C组和D组剪取跟骨-肌腱链作生物力学测试(再断裂应力)。结果显示电针治疗组A组组织学观察明显优于对照组B组,TGFβ1目标灰度值也明显优于B组,生物力学测试电针治疗组C组的再断裂应力指标明显优于D组,表明电针治疗可以促进跟腱断裂术后TGFβ1的表达,提高愈合跟腱的生物力学性能。     

COMPARISON OF EFFECTS OF ACUPUNCTURE ON CEREBRAL INFARCTION IN DIFFERENT PARTS

WithuniversalapplicationofCT(comput-erizedtomography)technique,diagnosticleve1ofcerebrovasculardiseasesincreasesgreatly.CTtechniquemakesdiagnosisofcerebralinfarctionofthecorticalsystemandthepenetratingbranchsystempossible.Inresentyearsstudieshaveprovedtha…     

EXPERIMENTAL STUDY OF ACUPUNCTURE IMPROVEMENT ON THE RESPONSE OF VACCINE IN AGING RATS

Objective: To observe the effects of Double-reinforcing and One-unblocking of acupuncture method on the immune response as the adjuvant in aging model rats induced by D-galactose injection.Methods: SD rats were randomly divided into four groups: model immune acupuncture group(A); model immune control group(B); normal immune acupuncture group(C); normal immune control group(D).Rats in group A and group B were injected with D-galactose for 6 weeks, and group C and group D were injected with the same amount of saline; all rats were injected with DTaP vaccine at the 7th week.Rats in groups A and B were treated with electroacupuncture and moxibustion for 3 weeks.At the 12 th week, the diphtheria antitoxin titer, the ratio of CD4+/CD8+ T cells of peripheral blood and the relative expression of spleen cells HSP70 mRNA were measured.Results: Compared with group D, CD4+/CD8+ T cells in group B was lower(P0.01), and the diphtheria antitoxin titer and HSP70 mRNA decreased significantly(P0.01).Compared with group B, the diphtheria antitoxin titer in group A increased significantly(P0.01), while CD4+/CD8+ T cells and HSP70 mRNA were higher(P0.05).The diphtheria antitoxin titer in group C was superior to that of group D, either.Conclusion: Acupuncture improved humoral and cellular immunity to the DTaP vaccine in aging rat probably by increasing the expression of endogenous adjuvant substances of HSP70.So our research probably suggests that acupuncture could be used as a novel adjuvant in vaccine research and even may provide a new field for the combination of Chinese and Western medicine research.     

针麻中电针刺激参数筛选的实验研究

<正> 目前针刺麻醉的临床实践和理论研究工作中,普遍采用针麻仪代替手法运针,作为维持穴位针感的刺激方法。临床实践中人们普遍地认为选用的电针刺激参数与针麻效果关系密切.因而合理地掌握刺激量是提高针麻效果的一个重要条件。这就要求我们对电针的刺激参数进行筛选,以求得针麻适宜的刺激条件.为此目的我们在北京大学高等数学教研组和生物系的指导下设计了如下一组动物实验来观察针麻仪(电针)主要的几种刺激参数对针麻效果的影响.     

A STUDY OF CHINESE ACUPUNCTURE IN PREVENTION OF METABOLIC SYNDROME

Objective: To investigate the effects of acupuncture in prevention of metabolic syndrome(MetS).Methods: Rats were divided into three groups: MetS group, control group and test group.The MetS and test groups were fed with high-lipid and high-glucose diet for 12 weeks.The test group was also treated with electroacupuncture since the start of the experimental diet.Food intake of the rats was noted daily.Body mass(BM), abdominal circumference and body length were measured weekly.After undergoing the experiment for 12 weeks, blood samples were taken from the orbital angular vein and tested for fasting plasma glucose(FPG), triglyceride(TG), total cholesterol(TC), high-density lipoprotein(HDL) and low-density lipoprotein(LDL).Results: Compared with the control group, food intake decreased from the 2nd week(P0.05) and abdominal circumference increased from the 4th week(P0.01) in the MetS group.At the end of the 12 th week, BM, TG, TC, LDL, and FPG were all higher than the control group(P0.05), while HDL was lower than the control group, but it was not statistically significant(P0.05).Food intake decreased from the 2nd week(P0.01) in the test group.At the end of the 12 th week, abdominal circumference, TC, LDL, and FPG were all higher than the control group(P0.05), while BM, TG, HDL, and FPG had no statistical difference(P0.05).Compared with the MetS group, food intake was lower and BM was smaller from the 8th week(P0.05) in the test group.At the end of the 12 th week, abdominal circumference, TG, TC, LDL, and FPG in the test group were lower than those in the MetS group, while HDL was higher than that in the MetS group, without any statistical significance(P0.05).Conclusion: Acupuncture interventions can reduce food intake in rats and delay the onset of MetS, thus havi ng a preventive effect.     

THE EFFECTS OF ACUPUNCTURE TREATMENT IN IMPROVING SHEN OF PRIMARY INSOMNIA PATIENTS

Objective: To investigate how acupuncture treatment affects the sleeping quality of insomnia patients.Methods: According to Doctor Zhou's experience on acupuncture treatment of insomnia, we chose Baihui, Sishencong, Shenting, Benshen, and Shenmen for the study.We chose estazolam as the contraposition group.We used the randomized control trail and single-blind method.We used the Pittsburgh Sleep Quality Index to evaluate the patients before and after the treatment, and the SPSS software for statistical analysis.Results: The study results reflected that acupuncture treatment had proved positive effect in regulating sleep quality of insomnia patients.For the primary insomnia patients, acupuncture played a better role in dealing with daytime function and sleeping obstacle.The study results reflected that the score of acupuncture group was significantly lower than the estazolam group.Conclusion: Acupuncture indeed provides a new way to cure insomnia, especially for patient group who suffers from nighttime sleeping obstacle and awakening difficulty during the day.     

摘除垂体对大鼠脑内5-羟色胺、去甲肾上腺素及针刺镇痛的影响

<正> 从往的实验表明,大鼠摘除垂体后,针刺镇痛效应明显减弱;摘除垂体后注射促肾上腺皮质激素(ACTH),针刺镇痛效应得到恢复。并认为ACTH恢复针刺镇痛效应的作用与皮质酮无关,从而提示,ACTH在针刺镇痛过程中可能具有中枢作用。因针刺镇痛与脑内5-羟色胺(5-HT)神经元及去甲肾上腺素(NE)神经元的神经传递密切相关,所以,进一步阐明垂体在针刺镇痛中的作用机制,显然是有意义的。本文采用摘除大鼠垂体的方法,观察对脑内5-     

INFLUENCE OF DIFFERENT INTERVALS OF ACUPUNCTURE TREATMENT ON BONE INTENSITY IN PRIMARY OSTEOPOROSIS PATIENTS

Primaryosteoporosisreferstooneoftheskeletaldiseasesmarkedbydecreaseofthebonequantity,micro structuraldegenerationofthebone,increaseofthebonebrittlenessandbeingliabletobebroken .Itisaphysiologicalretro gradeaffectionoccurringalongwiththeincreaseofpeople’sage[1 ] .Owingtothedecreaseofthebonequantityandtheincreaseofthebonebrit tleness,fractureisincreasinggreatly ,ifso ,thepatientwillhavetoberestrictedtothebedandmayexperiencesomeseverecomplications.Thus,nowadays,osteoporosisandtheresultantfractur…     

EXPERIMENTAL STUDY ON THE EFFECT OF ACUPUNCTURE ON TROPISM-DISTRIBUTION OF TETRAMETHYLPYRAZINE AND THE CONTENT OF cAMP IN ARTHRITIS RATS

“The application of combined Acupuncture andmedication”is recorded earlyinNeijing(《内经》TheYellow Emperor’s Internal Classic),in which it isadvocated that medicineisinternally used and needlesexternally used,and its superiority has been con-firmed by a great number of clinical studies.To in-vestigate the mechanisms of acupuncture combinedwith medication,the authors of this paper observedthe influence of acupuncture on the distribution ofspecific medicine in rats,and found that acupunctu…     

不同时辰电针大鼠“环跳”穴对血浆cAMP、cGMP水平的影响

<正> “子午流注”针灸疗法是中医颇具特色的治疗手段,临床实践表明疗效较好。用现代时辰生物学方法对这一独特疗法加以深入研究是有重要意义的。在许多细胞的生理活动中,cAMP,cGMP 是重要的第二信使,二者在细胞内的含量及其比例与细胞的功能状态有密切关系。已有文献报道人血浆 cAMP 水平存在近日节律性变化;另有报道表明血浆     

慢性乙型肝炎肝郁脾虚证生物信息学差异研究

目的:应用基因芯片技术研究慢性乙型肝炎肝郁脾虚证与它证的生物信息学差异。方法:应用Affymetrix Gene Chip人类全基因组芯片,分析了9例、3证型慢性乙型肝炎患者(肝肾阴虚型、湿热中阻型、肝郁脾虚型各3例)基因表达谱。通过应用Scanner 3000 7G 4C扫描仪对芯片进行扫描和信号值转换,获得各个中医证型之间的相对表达比值。结果:在检测的全部54676个基因中,中医证型基因表达存在着明显的差异,肝郁脾虚型与肝肾阴虚型、肝郁脾虚型与湿热中阻型比较,表达差异基因均占全基因的0.21%。结论:慢性乙型肝炎在肝郁脾虚证的基因层面上有着异常表达,这可能是临床出现同病异证的生物信息学基础,为其有效预防和治疗提供新的思路。     

寒痹康汤对实验性关节炎大鼠血清TNF—α的影响

目的：观察寒痹康汤对实验性关节炎大鼠的血清肿瘤坏死因子-α（TNF-α）的影响，探讨其作用机理。方法：采用牛Ⅱ型胶原蛋白诱导实验性关节炎模型，以甲氨喋呤作对照，观察大鼠的关节肿胀指数的变化，采用放射免疫法检测各组大鼠血清TNF-α水平。结果：寒痹康汤能显著减轻大鼠关节肿胀指数、显著降低大鼠血清TNF-α水平（P〈0．05或P〈0．01），其作用优于甲氨喋呤（P〈0．05或P〈0．01）。结论：寒痹康汤能下调实验性关节炎大鼠血清TNF-α的水平，是其治疗类风湿关节炎的作用机理之一。     

LOCAL EFFECTS OF ACUPUNCTURE ON THE MEDIAN AND ULNAR NERVES IN PATIENTS WITH CARPAL TUNNEL SYNDROME:STUDY DESIGN AND PRELIMINARY RESULTS

Objective: The purpose of this mechanistic study is to use electrophysiologic measurements, nerve conduction studies(NCS) and quantitative sensory testing(QST), to assess the local, nerve-specific effect of 3 acupuncture modalities on two anatomically distinct nerves in the forearm(median and ulnar nerve) in subjects with carpal tunnel syndrome(CTS).We expect to see a greater response in the diseased nerve(median), compared to the healthy one(ulnar).Methods: Participants with CTS were randomized to 3 intervention groups: manual, low- and high-frequency electroacupuncture.In week 1, baseline NCS and QST measurements were obtained in both nerve territories, followed by acupuncture to the median nerve(pericardium channel points) and to the ulnar nerve(heart channel points); one week apart, order counterbalanced.Acupuncture was followed by repeated NCS and QST measurements for both nerves.In week 2, repeated baseline QST and NCS measurements were obtained, followed by acupuncture to the nerve which was not treated in week 1, and by post-acupuncture NCS and QST measurements to both nerves.Results: Three pilot participants have been tested to date.In pilot 1, acupuncture caused 66% improvement of the median cold detection threshold, but not in the healthy ulnar nerve, with corresponding 60% increase in median compound muscle action potential(CMAP) amplitudes.In pilot 2, Median Sensory Nerve Action Potentials(SNAP) after acupuncturing, which had been missing at baseline due to CTS, was restored.In pilot 3, acupuncture caused improvement in median nerve cold(33%)and vibration(55%) sensation, with corresponding increases in median motor and sensory nerve conduction velocity(NCV).Ulnar sensory NCV and SNAP amplitude also increased with acupuncture.All 3 subjects reported symptomatic improvement.Conclusion: Acupuncture appears to have nerve-specific effect on the median and ulnar nerves in the forearm, which can be measured by NCS and QST.These effects will be fully assessed upon completion of the study which plans to include 36 participants.     

纳洛酮对不同强度电针所致镇痛效应的作用

<正> 脑内阿片受体及内啡肽系统的发现,对针刺镇痛原理研究提供了新的线索,关于针刺所致各种效应,特别是镇痛效应和阿片受体及其递质系统有何关系的问题,引起人们广泛的兴趣。Mayer 和上海市人体针刺镇痛实验协作组分别在人体用吗啡拮抗剂—纳洛酮阻断了针刺镇痛效应。然而动物实验的结果却存在分歧,Pomerang 报导纳洛酮能明显拮抗小鼠的电针镇痛作用;上