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1.
随着人类多瘤病毒的不断被发现,人们对它的研究兴趣也越来越大.自2007年人类WU多瘤病毒(WU polyomavirus,WUPyV)在呼吸道标本中被发现以来,世界各地陆续发表了发现WUPyV的报道.WUPyV在全世界广泛流行,可能在儿童时期获得感染,但大多数不表现临床症状.尽管WUPyV经常在呼吸道标本中发现,但是WUPyV是否为呼吸道疾病的病原还有争议.WUPyV的传播途径和流行性也有待进一步的研究.目前该病毒的流行病学资料主要来自PCR检测和血清学试验.本文现就WU多瘤病毒的研究进展作一综述.  相似文献   

2.
随着人类多瘤病毒的不断被发现,人们对它的研究兴趣也越来越大.自2007年人类WU多瘤病毒(WU polyomavirus,WUPyV)在呼吸道标本中被发现以来,世界各地陆续发表了发现WUPyV的报道.WUPyV在全世界广泛流行,可能在儿童时期获得感染,但大多数不表现临床症状.尽管WUPyV经常在呼吸道标本中发现,但是WUPyV是否为呼吸道疾病的病原还有争议.WUPyV的传播途径和流行性也有待进一步的研究.目前该病毒的流行病学资料主要来自PCR检测和血清学试验.本文现就WU多瘤病毒的研究进展作一综述.  相似文献   

3.
随着人类多瘤病毒的不断被发现,人们对它的研究兴趣也越来越大.自2007年人类WU多瘤病毒(WU polyomavirus,WUPyV)在呼吸道标本中被发现以来,世界各地陆续发表了发现WUPyV的报道.WUPyV在全世界广泛流行,可能在儿童时期获得感染,但大多数不表现临床症状.尽管WUPyV经常在呼吸道标本中发现,但是WUPyV是否为呼吸道疾病的病原还有争议.WUPyV的传播途径和流行性也有待进一步的研究.目前该病毒的流行病学资料主要来自PCR检测和血清学试验.本文现就WU多瘤病毒的研究进展作一综述.  相似文献   

4.
5.
目的 通过对呼吸道合胞病毒(RSV)及腺病毒(ADV)临床检测阳性率来评价儿童急性呼吸道感染病情严重程度的相关性分析.方法 将82例患急性呼吸道感染的儿童(感染组)及30例健康儿童(健康组)作为观察对象,比较两组RSV和ADV阳性率;同时根据感染严重程度,将感染组患儿分为28例的非肺炎组、33例的普通肺炎组及21例的重症肺炎组,对比三组间RSV和ADV阳性率.此外,对感染组患儿中RSV和ADV阳性率与感染严重程度、血常规白细胞计数(WBC)、中性粒细胞百分比(N%)及淋巴细胞百分比(L%)的相关性.结果 感染组RSV和ADV阳性率明显高于健康组(P <0.05),而不同严重程度感染组中,以重症肺炎组RSV和ADV阳性率最高(P<0.05),同时感染组中,RSV和ADV阳性率与患者的感染严重程度存在显著的直线相关性,但与WBC、N%及L%无直线相关性.结论 RSV和ADV阳性率可作为评价急性呼吸道感染患儿病情严重程度的指标.  相似文献   

6.
呼吸道合胞病毒(RSV)、流感病毒(Ⅳ)A、B型,副流感病毒(PIV)1、2、3型及腺病毒(ADV)是下呼吸道感染的常见病原。为了解浙江沿海地区急性下呼吸道感染患儿的病毒感染的状况,本文对我院2008年7月~2010年10月收住的301例小儿急性下呼吸道感染(ALRI)患儿进行了下呼吸道病毒病原检测,现报告如下。  相似文献   

7.
目的 分析重庆地区2008-2009年度急性呼吸道感染住院患儿呼吸道合胞病毒(respiratory syncytial virus,RSV)的亚型流行情况,并了解优势流行株BA株的G蛋白基因特征.方法 采集2008年4月-2009年3月全年于重庆医科大学附属儿童医院因急性呼吸道感染住院的508例患儿鼻咽深部分泌物,用RT-PCR方法检测RSV并进行亚型鉴定,选取29例B亚型和10例A亚型RSV阳性标本,用RT-PCR的方法扩增全长G蛋白并测序.结果 在508例标本中,RSV阳性126例(24.8%),其中检测出A亚型43例(34.1%),B亚型80例(63.5%),A、B亚型混合感染3例(2.4%).所测的10株A亚型的G基因与标准株A2的核苷酸同源性为91.4%~92.0%,均属GA2基因型;29株B亚型的G基因与标准株CH18537的核苷酸同源性为92.0%~93.0%,其中19株均为具有60个高度重复核苷酸插入的BA株.B亚型流行株与CH18537标准株相比,G基因有多种核苷酸变异如缺失、插入等,尤其在G蛋白近C端1/3处的高变区.结论 2008-2009年RSV仍是重庆地区儿童急性呼吸道感染的主要病原,与既往两年A亚型优势流行不同,2008-2009年度B亚型毒株流行占优;近年新发现的BA株可能已成为本地区优势流行株,BA株G基因变异是否导致G蛋白功能增强,进而促进其优势流行尚有待研究.  相似文献   

8.
急性呼吸道感染患儿巨细胞病毒感染的研究   总被引:2,自引:0,他引:2  
巨细胞病毒 (HCMV)在人群中感染非常普遍 ,但大多数呈亚临床不显性感染或潜伏感染 ,在宿主特殊的状态下 ,这种潜伏感染可被激活为其重要的生物学特征[1,2 ] 。为研究小儿急性呼吸道HCMV感染 ,根据 1994年全国小儿巨细胞病毒感染学术会议上拟定的诊断标准[3 ,4 ] ,选用HCMV IgM为HCMV活动感染指标 ,对 2 5 0例冬春季急性呼吸道感染 (ARI)患儿和 5 0名同期健康儿童进行了HCMV IgM、呼吸道合胞病毒IgM(RSV IgM) ,腺病毒IgM(AdV IgM)特异抗体同步检测和部分临床资料分析。1 材料和方法1.1 病…  相似文献   

9.
目的 了解乌鲁木齐地区冬春季节呼吸道感染患儿中呼吸道合胞病毒(RSV)的感染状况以及分子流行病学的基本情况.方法 研究对象为2006年11月-2007年4月于新疆维吾尔自治区人民医院儿科住院以及部分于门诊就诊,明确诊断为急性呼吸道感染的患儿,采集咽拭子280份,呼吸道分泌物112份.对全部标本采用反转录聚合酶链反应(RT-PCR)方法进行RSV及其亚型检测.随机取5份阳性标本测序,进行序列比较及同源性分析.结果 392份样本中共检出RSV阳性68份,阳性率17.3%(68/392),其中A基因型64份,占93.3%(64/68),B基因型4份,占6.7%(4/68).5株测序结果提示当地RSV与其他国家及地区代表株之间的同源性为63.1%~99.4%.进化树进一步显示存在A、B两个亚型.结论 RSV是引起2006-2007年冬春季该医院儿童急性呼吸道感染的重要病原体,以A亚型为主要流行株.
Abstract:
Objective To research the infections of respiratory syneytial virus(RSV)in children with respiratory tract inflammation and define its molecular epidemic features in Urumchi.Methods SamDles were collected from November 2006 to April 2007 in the People's General Hospital of Xinjiang Uygur Autonomous Region,including 112 respiratory secretions and 280 nasopharyngeal swabs. RSV and its subgroups were detected by nested PCR.The five positive amplicons selected randomly from all positive samples were sequenced and compared with other RSV in GenBank by BLAST and DNAStar.Results of all 392specimens.68 RSV G gene segments were tested.Among them,RSV lineage A occupied 93.3%,while B occuDied 6.7%.The identities between them were 63.1%-99.4%.Phylogenetic analysis defined that they belonged to two different clusters.Conclusion RSV was one of the important viruses leading to children's respiratory tract infections in the People's General Hospital of Xinjiang Uygur Autonomous Region during winter and spring from 2006 to 2007.RSV subtype A was the prevalent genotype in the hospital dunng this epidemics.  相似文献   

10.
目的 建立并应用检测儿童下呼吸道感染WU多瘤病毒(WU polyomavirus)的实时荧光定量PCR( real-time fluorescent quantitative PCR,FQ-PCR)方法.方法 选择WU多瘤病毒的VP2基因作为检测的目标基因,设计FQ-PCR引物和检测探针,以重组质粒为标准品建立标准曲线,并对该方法的特异性、灵敏度、重复性进行评价;应用该方法对温州医学院附属温岭医院收集的临床下呼吸道感染患儿的痰液、咽拭子846份及血清标本846份进行定量检测.结果 本研究建立FQ-PCR检测方法,质粒标准曲线的方差系数为0.998,灵敏度可达到50拷贝;应用该方法检测700份痰液标本,检测到7例阳性标本,146份咽试子标本中未检测到阳性标本,总阳性率为1.00% (7/700),846份血清标本未检测到阳性标本.结论 本研究建立的FQ-PCR方法可以特异、快速、灵敏地对儿童下呼吸道感染的WU多瘤病毒进行定量检测;痰液标本较咽拭子或血清标本更适用于WU多瘤病毒感染的核酸检测.  相似文献   

11.
The polyomaviruses KI (KIPyV) and WU (WUPyV) have recently been discovered in specimens from patients with respiratory tract infections. To analyze the frequency and clinical impact in a cohort of pediatric patients in a German University Children’s Hospital. Nasopharyngeal aspirates or bronchoalveolar lavage specimens of 229 children with acute respiratory tract infection were screened for KIPyV and WUPyV using polymerase chain reaction-based methods. KIPyV was detected in 2 (0.9%) and WUPyV in 1 (0.4%) patients, without co-infections with other respiratory viruses but with co-detection of CMV, EBV and HHV 6 in one immunocompromised patient. Only a very small proportion (1.3%) of positive samples for KIPyV and WUPyV was documented in this study; the clinical relevance of these viruses remains unclear and requires further evaluation.  相似文献   

12.
BackgroundWU and KI are human polyomaviruses initially detected in the respiratory tract, whose clinical significance remains uncertain.ObjectivesTo determine the epidemiology, viral load and clinical characteristics of WU and KI polyomaviruses.Study designWe tested respiratory specimens collected during a randomized, placebo-controlled pneumococcal conjugate vaccine trial and related epidemiological study in the Philippines. We analyzed 1077 nasal washes from patients aged 6 weeks to 5 years who developed lower respiratory tract illness using quantitative real-time PCR for WU and KI. We collected data regarding presenting symptoms, signs, radiographic findings, laboratory data and coinfection.ResultsThe prevalence and co-infection rates for WU were 5.3% and 74% respectively and 4.2% and 84% respectively for KI. Higher KI viral loads were observed in patients with severe or very severe pneumonia, those presenting with chest indrawing, hypoxia without wheeze, convulsions, and with KI monoinfection compared with co-infection. There was no significant association between viral load and clinical presentation for WU.ConclusionsThese findings suggest a potential pathogenic role for KI, and that there is an association between KI viral load and illness severity.  相似文献   

13.
The KI and WU polyomaviruses were found in 11 (2.7%) and 17 (4.2%) of 406 nasopharyngeal aspirates, respectively, from children with acute respiratory tract infection (ARTI). The phylogenetic analysis indicates that they are all in the same cluster as the prototype strains. Our findings suggest that they are common in children with ARTI in China.  相似文献   

14.
BACKGROUND: WU polyomavirus (WUPyV), a new member of the genus of Polyomavirus in the family Polyomaviridae, has been found and associated with respiratory tract infections recently. However, its clinical role and pathogenicity has not been known. OBJECTIVES: To confirm that WU polyomavirus has been found in Chinese children. STUDY DESIGN: WU polyomavirus was detected and identified using PCR methods. A total of 278 specimens of nasopharyngeal aspirate were collected, and then PCR products were sequenced directly. RESULTS: One of 278 nasopharyngeal aspirates was positive for WUPyV in one child, and the positive rate was 0.4%. The results showed that the sequences of genome, LTAg and VP2 gene was identical to the reference sequences of WU polyomavirus prototype strains. CONCLUSIONS: We confirmed that WU polyomavirus had been found and identified in the respiratory secretions in China.  相似文献   

15.
BACKGROUND: The role of two recently identified polyomaviruses, KI and WU, in the causation of respiratory disease has not been established. OBJECTIVES: To determine the prevalence of KI and WU viruses (KIV and WUV) in 371 respiratory samples and evaluate their contribution to respiratory disease. STUDY DESIGN: Specimens were screened for KIV and WUV using single, multiplex or real time PCR; co-infection with other respiratory viruses was evaluated. RESULTS: Of the 371 samples analysed, 10 (2.70%) were positive for KIV and 4 (1.08%) were positive for WUV yielding an overall case prevalence of KIV and WUV infection of 3.77%. KIV and WUV were identified in patients aged <15 years (11 patients) with upper or lower respiratory tract infection and >45 years (3 patients) with upper respiratory tract infection. Co-infections were found in 5 (50%) and 3 (75%) of the KIV and WUV positive samples, respectively. CONCLUSIONS: This study supports previous conclusions that KIV and WUV detection in the respiratory tract may be coincidental and reflect reactivation of latent or persistent infection with these viruses. The age distribution of KIV and WUV infection in this study mirrors that found for the other human polyomaviruses, BK and JC.  相似文献   

16.
This study aimed at investigating the prevalence and clinical characteristics of children with respiratory infection by WU polyomavirus (WUPyV) in Southern China. Nasopharyngeal aspirate samples were collected from 771 children with acute respiratory tract infection admitted to hospital and 82 samples from healthy subjects for routine examination at the outpatient service at the Second Affiliated Hospital of Shantou University, Medical College from July 2008 to June 2009. WUPyV was detected by the polymerase chain reaction (PCR) and DNA sequencing. All WUPyV‐positive specimens were characterized further for nine viruses causing common respiratory infections, including in?uenza A and B, respiratory syncytial virus (RSV), parainfluenza virus (PIV) 1 and 3, human metapneumovirus, human bocavirus, adenovirus, and rhinovirus by PCR or real time (RT)‐PCR. Fifteen out of 771 specimens from patients with acute respiratory tract infection, but none from healthy subjects, were positive for WUPyV and the positivity rate was 2%. Patients with WUPyV infection were between 2 and 48 months of age, and nine of the patients were male while six female. Four out of 15 patients were co‐infected with RSV, one with adenovirus or rhinovirus, respectively. Patients with WUPyV infection displayed predominantly cough, moderate fever, and wheezing, and were diagnosed with pneumonia (n = 8), bronchiolitis (n = 4), upper respiratory tract infections (n = 2) and bronchitis (n = 1). One patient developed encephalitis. Therefore, WUPyV infection can cause acute respiratory tract infection with atypical symptoms, including severe complications, in children. J. Med. Virol. 83:1440–1445, 2011. © 2011 Wiley‐Liss, Inc.  相似文献   

17.
BACKGROUND: Currently, the role of the novel human polyomaviruses, KI (KIV) and WU (WUV) as agents of human disease remains uncertain. OBJECTIVES: We sought to determine the prevalence of these viruses and their rate of co-detection with other viral respiratory pathogens, in an Australian population. STUDY DESIGN: Polymerase chain reaction assays previously described were used to examine the presence of KIV and WUV in 2866 respiratory specimens collected from January to December 2003 from Australian patients with acute respiratory infections. RESULTS: KIV and WUV were present in our population with an annual prevalence of 2.6% and 4.5%, respectively. There was no apparent seasonal variation for KIV, but a predominance of infection was detected during late winter to early summer for WUV. The level of co-infection of KIV or WUV with other respiratory viruses was 74.7% and 79.7%, respectively. Both viruses were absent from urine and blood specimens collected from a variety of patient sources. CONCLUSIONS: KIV and WUV circulate annually in the Australian population. Although there is a strong association with the respiratory tract, more comprehensive studies are required to prove these viruses are agents causing respiratory disease.  相似文献   

18.
A novel polyomavirus (WU virus) has been identified in pediatric patients with acute respiratory tract infections (ARI), but its role as a respiratory pathogen has not yet been demonstrated. To investigate if WU virus is related to acute respiratory infections in infants and children in Beijing, specimens collected from 674 pediatric patients with ARI from April 2007 to May 2008 and from 202 children without ARI were used for this investigation. Common respiratory viruses were tested by virus isolation and/or antigen detection by indirect immunofluorescent assay followed by RT-PCR or PCR for other viruses associated with respiratory infections in specimens collected from patients with ARI before WU virus DNA was detected. WU virus DNA was detected by initial screening and secondary confirmation PCR for all specimens. The region encoding the VP2 gene of the virus was amplified from 17 WU-virus-positive clinical specimens, and sequence analysis was performed. Thirty-eight of 674 (5.6%) specimens from patients with ARI and 3 of 202 (1.5%) specimens from children without ARI yielded PCR products with the predicted molecular weight, using either screening or confirmation primer sets, indicating that these specimens were WU virus positive. However, more than 60% of the 38 WU-virus-positive specimens from patients with ARI were also positive for one or more respiratory viruses. The nucleotide and deduced amino acid sequences of the region encoding the VP2 gene from 17 Beijing WU viruses shared high homology (>98.5%) with sequences from GenBank and among themselves. The data indicated that WU virus in Beijing occurred 3.7 times more frequently in pediatric patients with ARI than in those without ARI (p < 0.05).  相似文献   

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20.
Human bocavirus (HBoV) is a recognized human parvovirus associated with acute respiratory tract infection. However, HBoV has yet to be established as a causative agent of respiratory disease. In this study, the epidemiological and virological characteristics of HBoV infection were studied in children with acute respiratory tract infection in China. In total, 406 children younger than 14 years of age with acute respiratory tract infection were included in this prospective 1‐year study. HBoV was detected in 29 (7.1%) of the 406 children. No clear seasonal fluctuation was observed in infection rates of HBoV. Of the 29 children infected with HBoV, 16 (55.2%) were coinfected with other respiratory viruses, most commonly respiratory syncytial virus (RSV). Viral coinfection with HBoV did not affect the severity of the respiratory disease (P = 0.291). The number of HBoV genome copies ranged from 5.80 × 102 to 9.72 × 108 copies/ml in nasopharyngeal aspirates among HBoV‐positive specimens by real‐time PCR, and neither coinfection nor the severity of disease correlated with the viral load (P = 0.148, P = 0.354, respectively). The most common clinical features were cough and acute upper respiratory infection, and acute bronchopneumonia. Additionally, the NP‐1 gene of HBoV showed minimal sequence variation. These data suggest that HBoV is frequent in young children with acute respiratory tract infection in Lanzhou, China, and RSV is the most common coinfecting virus. There was no apparent association between the viral load of HBoV and coinfection or disease severity. The NP‐1 gene was highly conserved in HBoV. J. Med. Virol. 82:282–288, 2010. © 2009 Wiley‐Liss, Inc.  相似文献   

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