Prime&Bond(R)NT粘接剂治疗牙本质过敏症的临床观察

目的探讨有效治疗牙本质过敏症的方法。方法将120例患者的356颗牙本质过敏牙随机分为实验组和对照组,每组178颗。实验组用Prime＆BondNT粘接剂脱敏,对照组用脱敏糊剂脱敏。观察两组牙齿脱敏治疗的即刻、3个月后和半年后的临床效果。结果在脱敏治疗的即刻、3个月后和半年后,实验组患牙牙本质过敏症的治疗效果都优于对照组,实验组治疗牙本质过敏症的有效率超过94%。结论Prime＆BondNT粘接剂是一种治疗牙本质过敏的理想脱敏剂。     

牙本质粘接剂盖髓的研究进展

盖髓剂的选择是盖髓术成功与否的一个重要方面，目前已有多种材料被用作盖髓剂。本文对牙本质粘接剂用作间接盖髓剂的粘接原理、屏障作用、抗菌作用及细胞毒性；用作直接盖髓剂的生物相容性和止血作用等进行综述。     

牙本质粘接剂盖髓的研究进展

盖髓剂的选择是盖髓术成功与否的一个重要方面,目前已有多种材料被用作盖髓剂。本文对牙本质粘接剂用作间接盖髓剂的粘接原理、屏障作用、抗菌作用及细胞毒性;用作直接盖髓剂的生物相容性和止血作用等进行综述。     

纤维蛋白粘接剂盖髓的动物实验研究

目的：观察纤维蛋白粘接剂（ＦＳ）对人工暴露的牙髓组织愈合的影响。方法：采用ＦＳ行狗牙直接盖髓术,常规制备牙－颌骨联合切片,光学显微镜观察牙髓创面的愈合及牙本质的修复。结果：ＦＳ盖髓后牙髓无出血,炎性反应轻。氢氧化钙盖髓后牙髓出血多,炎性反应较重。ＦＳ组术后４２ｄ出现骨样牙本质桥修复,氢氧化钙组术后２８ｄ出现骨样牙本质桥修复,术后６３ｄ,ＦＳ组６例中４例有牙本质桥形成,氢氧化钙组６例均有牙本质桥形成。结论：ＦＳ虽无牙本质诱导活性但因其良好的保护及促进牙髓组织创面愈合能力仍不失为一种较为理想的盖髓剂。     

四种牙本质粘接系统的微拉伸强度研究

目的　评价四种牙本质粘接系统 (SingleBond ;Bond 1;Prime&BondNT ;One Step)的微拉伸粘接强度。方法　选择新鲜拔除的无龋磨牙 2 0个 ,磨除牙釉质 ,暴露牙本质。分别用四种牙本质粘接系统按使用说明进行粘接处理。牙齿贮存于 3 7℃生理盐水 2 4h后 ,制备成沙漏状试件。粘接界面面积控制在 1mm2 左右。测试其拉伸强度 ,并作统计学处理。结果　四种牙本质粘接系统的微拉伸强度分别为 :2 5 .80± 2 .41Mpa (SingleBond) ;2 9.92± 3 .0 4Mpa (Bond 1) ;2 8.97± 2 .73Mpa (Prime&BondNT) ;3 0 .0 3± 2 .96Mpa (One Step)。结论　微拉伸方法测得的粘接强度明显高于传统较大粘接面积时的测试值。更为客观、真实地反应了各粘接系统的粘接强度     

三种牙本质粘接剂强度的体外测量与比较

目的为选择适用于折裂牙接合的材料,对常用的三种牙本质粘结剂进行了体外测量和比较。方法选用拔除的36颗人磨牙随机分为3组,制备成纵裂模型,颊侧半剖面牙本质上粘结树脂块,3组粘结系统分别为ClearfilSEBond、SingleBond、SuperBondC&B。标本置370C水中恒温24h后,在万能材料试验机上以1mm/min的横梁移动速率做剪切力测试。试件断裂方式用扫描电镜观察。结果用剪切法所测得的3组粘结剂强度分别为:ClearfilSEBond:(15.3±0.53)MPa;SingleBond:(10.3±1.49)MPa;SuperBondC&B:(12.3±0.82)MPa,经方差分析,3组间差异有统计学意义(P<0.05)。75%以上试件断裂为界面破坏,其余伴有混合破坏,卡方检验,3组无显著性差异(P>0.05)。结论3组粘结系统中,ClearfilSEBond具有相对较高的粘结强度。     

MTA直接盖髓的动物实验研究

目的 比较MTA（mineraltrioxideaggregate）及氢氧化钙犬牙直接盖髓后牙髓的反应。方法 将三只健康雄性杂种犬共72颗牙齿机械开髓，然后用MTA和氩氧化钙分别盖髓。窝洞用玻璃离子水门汀充填。在5，7，9个月后。对实验牙齿进行组织切片制备，借助计算机图文处理系统对所形成的修复性牙本质宽度进行测量，对成牙本质细胞、牙髓细胞及毛细血管进行计数、分析。结果 5个月后，MTA及氢氧化钙盖髓效果相似。在7，9个月后。MTA组牙髓较氢氧化钙组可见更明显的修复性牙本质形成，更多的成牙本质细胞和牙髓细胞。结论 MTA具有临床活髓保存治疗应用价值。     

Prime ＆ Bond NT粘结剂对银汞合金充填微渗漏及密合度的影响

目的探讨Prime&Bond NT粘结剂对银汞合金充填微渗漏及密合度的影响.方法前磨牙120个,随机分为2组,分别进行微渗漏实验及密合度实验.每组中又分为空白对照组、EDTA组和实验组.每个样本的中央制备深3 mm、直径2 mm的窝洞,空白对照组进行常规银汞合金充填;EDTA组使用10%EDTA处理窝洞后充填银汞合金;实验组在EDTA组的处理基础上采用Prime&Bond NT粘结剂进行粘结性充填,分别采用染料渗入法及扫描电镜进行微渗漏及密合度观察.结果实验组的微渗漏率及洞壁与充填体间的间隙明显小于EDTA组(P<0.01);EDTA组又小于空白对照组(P<0.05).结论Prime&Bond NT粘结剂能增加洞壁与银汞合金充填体间的密合度,减少微渗漏.     

Prime & Bond®NT粘接剂治疗牙本质过敏症的疗效观察

目的观察Prime & Bond     

ONE-STEP粘接剂治疗牙本质过敏症的临床疗效

目的 :评价ONE -STEP粘接剂对牙本质过敏症脱敏治疗的疗效。方法 :牙本质过敏症患牙 3 3 6个 ,随机分成 3组。A组 :ONE -STEP粘接剂脱敏 ,B组 :Gluma脱敏剂脱敏 ,C组 :氟化钠甘油糊剂脱敏。结果 :即刻疗效 :A组 >C组 (P <0 .0 1)、B组 >C组 (P <0 .0 1)、A组与B组无差异 (P >0 .0 5 )。 1个月和 3个月疗效 :A组 >B组 (P <0 .0 1)。结论 :ONE -STEP粘接剂治疗牙本质过敏症疗效稳定持久。     

牙本质黏结剂对牙髓直接影响的动物实验

目的：观察两种牙本质黏结剂对活体牙髓组织的影响，并与氢氧化钙直接盖髓结果进行对比。方法：选用54个犬牙，机械穿髓后，无菌蒸馏水冲洗，控制穿髓孔处的出血和渗出。使用优邦(UB)和Adper Prompt Self-etching Adhesive(AP)两种牙本质黏结剂盖髓，Dycal作对照。于7、30、90d拔除实验牙，脱钙切片，HE染色、Mallory三色法染色和Gram快速细菌染色检查?结果：所有处理组在短期都出现了轻到中度的炎症反应，在长期的观察中，UB和AP两组出现持续的慢性炎症反应，均未发现牙本质桥肜成。Dycal组出现更多的修复性牙本质，并有牙本质桥形成(P＜0．05)结论：这两种牙本质黏结剂会引起牙髓的慢性炎症反应，在穿髓孔不能形成完整的牙本质桥，临床上这两种黏结剂不能直接与牙髓接触。     

应用Prime＆BondNT防止髓病治疗后牙冠变色的初步探讨

目的　初步探讨防止髓病治疗后牙冠变色的方法。方法　选择 60颗需做牙髓摘除术的前牙 ,随机分成实验组和对照组 ,每组均为 3 0颗牙 ,实验组在封CP棉捻前先用Prime&BondNT作髓室内粘结封闭 ,而对照组则直接封药。两组牙齿均在封CP棉捻前用Ivoclar比色板记录牙冠的色泽 ,嘱 6月、1 5年后复诊 ,再次记录其色泽。结果　治疗 6月时 ,两组牙冠的变色无显著性差异 (P >0 0 5 ) ;而治疗 1 5年时复诊 ,两组牙冠变色有显著性差异 (P <0 0 5 )。结论　Prime&BondNT能有效封闭髓室内牙本质小管 ,防止有色物质的渗入 ,减少髓病治疗后牙冠的变色。     

牙本质表面不同处理方法对粘接剂与牙本质间剪切强度的影响

目的研究氧化锌丁香酚黏固剂和Gluma脱敏剂对牙本质与磷酸锌黏固剂、玻璃离子黏固剂和Super- BondC&B树脂粘接剂间剪切强度的影响,为临床上牙体制备后应用不同的保护方法及选用不同类型的粘接剂提供理论依据。方法90颗前磨牙在流水冲洗下去除颊面釉质暴露浅层牙本质。依次用粒度为320、400、600目的水砂纸磨平牙本质粘接面。牙根用自凝树脂包埋,牙冠暴露。包埋好的90颗牙齿随机分为3个大组,分别为：A组为对照组,牙本质表面不作任何处理;B组牙本质表面用氧化锌丁香酚黏固剂均匀涂抹一薄层;C组用Gluma脱敏剂均匀涂抹一薄层。测试两种处理剂处理牙本质表面后,3种粘接剂与牙本质间剪切强度的大小,进行统计分析及扫描电镜观察。结果应用两种处理剂后磷酸锌黏固剂与牙本质间剪切强度明显下降（P<0.05）,C1组下降更明显;玻璃离子黏固剂和Super- Bond C&B树脂粘接剂与牙本质间剪切强度没有明显变化。结论两种处理剂均对磷酸锌黏固剂与牙本质间粘接强度有不利影响;对玻璃离子黏固剂和Super- Bond C&B树脂粘接剂与牙本质间粘接强度无不利影响。     

Factors affecting in vitro bond strength of bonding agents to human dentin

Four generations of total-etch (fourth, fifth) and self-etching (sixth, seventh) bonding agents for use with resin composites are commercially available in the United States. Innovations in bonding agents include: filled systems, release of fluoride and other agents, unit dose, self-cured catalyst, option of etching with either phosphoric acid or self-etching primer, and pH indicators. Factors that can affect in vitro bond strength to human dentin include substrate (superficial dentin, deep dentin; permanent versus primary teeth; artificial carious dentin), phosphoric acid versus acidic primers, preparation by air abrasion and laser, moisture, contaminants, desensitizing agents, astringents, and self-cured restorative materials. This article reviews studies conducted at the Houston Biomaterials Research Center from 1993 to 2003. Results show that in vitro bond strengths can be reduced by more than 50% when bonding conditions are not ideal.     

Electron microscope characteristics of dentin repair after hydroxylapatite direct pulp capping in rats

In order to study the osteogenic action of hydroxylapatite (HA) on the dental pulp, a pulp capping experiment was designed using the rat upper molar. Under general anesthesia, molar teeth in 14 male Sprague-Dawley rats were pulp capped with Osteogen (HA) or with Dycal as a control material. After pulp capping, the maxillary molars cavities were restored with amalgam and a pedodontic steel crown was adjusted and sealed over the molar teeth on either side of the maxilla. After 7 days, the areas of necrosis and acute inflammation were more evident in the pulps treated with Dycal than with Osteogen. Hard tissue formation began to appear around dentinal chips in the pulp and extended from the cavity walls into the pulp regardless of the material that was used. Furthermore, this calcified material was scattered throughout the pulp when Osteogen was used, but was not observed in the Dycal treated pulps. The hard tissue formation was thought to be due to the putative fibroblasts and odontoblasts found in the pulp. After 28 days dense dentinal tissue was observed bridging the exposure site when Dycal was used. The dentinal tissues formed with Osteogen was always of a globular type, and showed an irregular distribution. Since Osteogen tends to cause areas of dystrophic calcification in the pulp, its use is not be recommended for pulp capping purposes in humans, because these areas of calcification would make future endodontic treatment difficult.     

两种脱敏剂对牙本质粘接剂粘接强度的影响

目的 探讨两种脱敏剂对牙本质粘接剂粘接强度和微观形态的影响,以期对临床治疗有所指导.方法 将30颗完整且无龋的人第三磨牙去除(牙合)面釉质,采用随机数字表法随机分为3组:C组(对照组)、U组(UltraEZTM处理)和M组(MI Paste处理);每组10颗牙齿.C组不做处理,U组和M组分别使用相应脱敏剂对暴露的牙本质表面进行处理.3组样本牙再各随机分为两个小组,每小组5颗牙,分别用粘接剂A(Single Bond 2)和B(Xeno Ⅲ)粘接,用复合树脂修复达4～5 mm.用慢速锯将牙齿切成粘接面积为0.9 mm×0.9 mm的长方体试件,用微拉伸测试仪测试微拉伸强度,作为粘接强度,对其进行单因素方差分析.每组随机挑选3个试件对粘接界面进行扫描电镜观察.结果 对于粘接剂A,U组粘接强度[(14.58±2.31)MPa]与C组[(15.82±2.18)MPa]的差异无统计学意义(P>0.05),而M组粘接强度[(9.90±0.79)MPa]降低.对于粘接剂B,U组粘接强度[(10.55±1.06)MPa]与C组[(10.73±1.07)MPa]的差异无统计学意义(P>0.05),而M组粘接强度[(8.74±0.87)MPa]降低.扫描电镜见各组混合层均较致密,M组两种粘接剂的树脂突较短且稀少.结论 两种脱敏剂对粘接剂粘接强度的影响不同,U组脱敏剂对全酸蚀和自酸蚀粘接剂的粘接强度无明显影响,而M组脱敏剂可降低两种粘接剂的粘接强度.     

2种盖髓剂行大鼠磨牙直接盖髓后转化生长因子β1表达的比较

目的: 观察氢氧化钙(calcium hydroxide, CH)和矿物三氧化物凝聚体(mineral trioxide aggregate, MTA)行大鼠磨牙直接盖髓后转化生长因子β1(transforming growth factor-β1, TGF-β1)的表达变化, 比较2种盖髓剂对牙髓损伤修复中TGF-β1表达的影响。方法: 建立28只雌性大鼠CH和MTA第一磨牙直接盖髓模型, 在观察时间点1、3、5、7、14、21、28 d处死大鼠取材, 进行TGF-β1免疫组化染色, 用图像分析软件IPP6.0测定各组标本中TGF-β1阳性染色的平均吸光度值。采用SPSS 13.0软件包对数据进行Dunnett t检验和配对t检验。结果: 在正常牙髓组织中, TGF-β1基本无表达;CH和MTA直接盖髓后不同时间, TGF-β1表达强度呈先升高后降低的变化, 其中, 盖髓后5 d的TGF-β1表达最强, 21 d时接近正常水平。表达主要位于盖髓剂下方的成纤维细胞、成牙本质细胞、血管内皮细胞、中性粒细胞等。2种盖髓剂在盖髓后1、3、5、7、14 d , TGF-β1的表达强度有显著差异(P<0.05)。结论: 盖髓剂直接盖髓后, TGF-β1表达强度呈先升高后降低的变化, 不同盖髓剂直接盖髓对牙髓损伤修复中TGF-β1的表达有一定影响。在直接盖髓后的1、3、5、7、14 d, MTA比CH更能促进TGF-β1的表达。     

Bond strength of dentinal bonding agents to chemomechanically prepared dentin

The aim of this investigation was to compare the shear bond strengths of 5 commercially available dentinal bonding agents to remaining dentin after chemomechanical caries removal (Caridex^TM) with the strengths obtained after caries removal with conventional mechanical drilling; and to examine the dentin/resin interface. One hundred freshly extracted carious teeth were randomly assigned for caries removal with either the chemomechanical technique or with conventional mechanical drilling. Caries removal was continued until the remaining dentin surfaces were judged sound. In addition, 50 sound teeth were ground on 600 grit SiC to provide a control group. Groups of 10 teeth were assigned for bonding with each of the 5 dental bonding agents. All bonded specimens were stored in water at 37°C for 24 h. Shear bond strength was tested using an Instron testing machine at a cross-head speed of 0.05 cm/min. Analysis of variance showed that the 5 materials tested produced significantly different bond strengths (p<0.001). For the 5 dentinal bonding agents evaluated, chemomechanically-treated dentin provides at least comparable, and probably superior, 24 h bond strengths compared with dentin that has been mechanically prepared.     

粘接处理剂对自粘接树脂水门汀和RMGIC的牙本质粘接强度的影响

目的:实验评价牙本质粘接处理剂对自粘接树脂水门汀和树脂加强型玻璃离子水门汀(RMGIC)的牙本质微拉伸粘接强度的影响.方法:选用离体人无龋第三恒磨牙24颗,用低速切片机垂直于牙体长轴方向将磨牙冠(牙合)中1/3交界线处切开待用.实验组牙本质表面涂布牙本质粘接处理剂,对照组不涂粘接处理剂.后将试样分别用自粘接树脂水门汀(Unicem,3M ESPE;seT PP,SDI)或树脂加强型玻璃离子水门汀(Fuji CEM,GC)原位对位粘接.水浴中储存24h后,用低速切片机把样本切割成约1mm×1mm×8mm条状,随后进行微拉伸测试.用扫描电镜观察粘接界面形貌.结果:无论是否使用粘接处理剂,Unicem的牙本质粘接强度显著高于seT PP 和Fuji CEM(P <0.01).与对照组相比,实验组的粘接强度显著提高(P <0.05).结论:粘接处理剂表面处理增强自粘接树脂水门汀及树脂加强型玻璃离子水门汀的牙本质粘接强度.     

The effect of four materials on direct pulp capping: An animal study

The aim of this study was to investigate the outcomes of direct pulp capping performed with two types of tricalcium silicate‐based materials (mineral trioxide aggregate/MTA and Biodentine/BD); nano‐hydroxyapatite (nHAP) crystals or calcium hydroxide (CH) in dogs. Following mechanical exposure, the pulps were randomly capped with one of the four materials. Histological analyses were performed to examine the outcomes after 7 days or 3 months. At 7 days, BD and nHAP showed significantly less inflammatory cell response than MTA and CH. At 3 months, the inflammatory cell response and tissue necrosis were significantly higher in the CH group. There was no significant difference between the tested materials in the calcific bridge formation after 7 days; however, a significant difference was noticed at the 3‐month period. Tricalcium silicate‐based cements and nHAP are potential alternatives to CH in vital pulp therapy following accidental pulp exposure.