关节镜下股骨双隧道Y型LARS人工韧带移植重建PCL的临床疗效

目的评价关节镜下股骨双隧道Y型LARS人工韧带移植重建PCL的临床疗效。方法对60例行关节镜下股骨双隧道Y型LARS人工韧带移植重建PCL患者的临床资料做详细回顾性分析,观察并记录患者治疗前后膝关节功能评分、相关并发症发生情况。结果全部患者术后愈合良好,伤口愈合均达甲级;无韧带断裂或松动,慢性滑膜炎、活动受限等并发症发生。手术前患者膝关节功能平均评分为(42.13±8.6)分,手术后为(83.26±3.7)分;手术后患者膝关节功能评分明显提高(P<0.01)。结论关节镜下股骨双隧道Y型LARS人工韧带移植重建PCL治疗效果显著,可有效改善患者膝关节功能,减少相关术后并发症的发生,具有临床推广价值。     

关节镜下lars韧带重建后交叉韧带中期疗效观察

目的:探讨关节镜下LARS人工韧带重建后交叉韧带(PCL)中期疗效。方法:17例陈旧性PCL损伤患者在伤后2～5个月使用LARS人工韧带(PC80)作PCL重建。结果:17例均获随访,时间36～48个月,膝关节稳定性良好,膝活动度较术前改善,无伸曲受限,后抽屉试验、反轴移试验均为阴性,Lyshlom评分由术前45.31±5.34分提高到术后90.29±1.50分,两组比较差异有显著性(P<0.05);Tegner评分由术前3.2±0.6提高至术后5.9,两组比较差异有显著性(P<0.01),未发生并发症。结论:关节镜下LARS人工韧带重建PCL术中操作简便,创伤小,效果可靠。     

关节镜下应用LARS人工韧带重建膝关节交叉韧带

目的本文探讨75例患者前、后交叉韧带分别或同时损伤后对其进行关节镜下应用LARS人工韧带进行重建的手术方法及其疗效。方法关节镜下重建前交叉韧带患者37例;后交叉韧带患者20例,同时重建前、后交叉韧带损伤患者18例,重建材料采用LARS人工韧带。术后随访10~42个月,采用国际膝关节文件委员会韧带标准评价表(IK-DC)和Lysholm膝关节功能评分表评估患膝功能,通过KT-1000检查膝关节前后松弛度。结果重建前交叉韧带,后交叉韧带,同时重建前后交叉韧带;所有患者术前Lysholm膝关节功能评分及IKDC评分与术后评分差异均有统计学意义。结论关节镜下应用LARS人工韧带重建前交叉或后交叉韧带,或者同时重建膝关节前、后交叉韧带,是目前治疗膝关节交叉韧带损伤的一种安全、有效的微创手术方法,近期疗效佳。     

人工韧带与自体肌腱重建前交叉韧带的meta分析

目的 系统评价LARS人工韧带及自体移植物在前交叉韧带重建中的安全性和有效性.方法 通过检索PubMed数据库、中国学术期刊全文数据库(CNKI)、中国生物医学文献数据库(CBMdisc)、万方数据库获得已公开发表的LARS人工韧带与自体移植物进行前交叉韧带重建的文献.提取相关数据进行meta分析.结果 本研究共纳入了9篇文献,总病例数456例,结果显示LARS人工韧带术后患者膝关节Lysholm评分及Tegner评分较术前有明显提高(Lysholm:MD=50.05,95 ％CI 48.41～51.68;Tegner:MD=4.41,95％CI 3.40～5.42);并且在术后2年其Lysholm评分及Tegner评分改善较自体肌腱移植仍更明显(Lysholm:MD=0.20,95％CI 0.04～0.35;Tegner:MD=0.18,95％ CI 0.04～0.32).同时,术后2年LARS人工韧带组滑膜炎等并发症发生率低,与自体移植物无明显差异.结论 LARS人工韧带具有良好的临床疗效和安全性,并且在术后2年其稳定性较自体肌腱移植仍具有明显优势,但该结论仍需长随访、高质量的临床研究进一步证实.     

关节镜下单束四股自体肌腱移植重建前交叉韧带股骨隧道改良取点法的临床效果

目的观察关节镜下单束四股自体肌腱移植重建前交叉韧带股骨隧道改良取点法的临床效果。方法选取2013年5月至2018年3月濮阳市人民医院收治的150例前交叉韧带断裂患者,按照定位方法分为改良重建组、等长重建组、解剖重建组,各50例。解剖重建组接受关节镜下股骨止点解剖重建,等长重建组接受关节镜下自体腘绳肌腱单束等长重建,改良重建组接受关节镜下单束四股自体肌腱移植股骨隧道改良取点法重建。对比3组术前、术后6、12个月膝关节功能恢复情况[国际膝关节文献委员会(IKDC)评分]、膝关节韧带损伤情况(Lysholm膝关节评分)。结果术后6、12个月,改良重建组Lysholm膝关节评分均高于等长重建组和解剖重建组,差异有统计学意义(均P<0.001);术后6、12个月,改良重建组IKDC评分均高于等长重建组和解剖重建组,差异有统计学意义(均P<0.001)。结论对前交叉韧带断裂患者采用关节镜下单束四股自体肌腱移植股骨隧道改良取点法重建,可促使患者韧带、膝关节功能恢复。     

关节镜下LARS人工韧带重建后交叉韧带的临床应用

目的：探讨关节镜下运用韧带增强重建系统（ligament advanced reinforcement system,LARS）人工韧带重建后交叉韧带（PCL）的方法与疗效。方法：2007年4月～2008年12月对12例后交叉韧带撕裂伤的患者施行人工韧带重建术,其中,单纯PCL撕裂伤患者10例,ACL、PCL同时撕裂伤患者2例,合并内侧副韧带损伤3例。所有患者均给予LARS人工韧带重建术。手术时间30～90min。术后膝关节放置引流管,单纯后交叉韧带撕裂不合并其余结构损伤者术后不做外固定,而合并有侧副韧带损伤患者术后给予石膏外固定3周,拆除外固定后开始屈伸关节锻炼。结果：术后所有患者经过6～21个月随访,术后膝关节不稳感消失。术后6个月Lysholm评分平均95分,患者膝关节功能恢复好,疗效满意。结论：在关节镜下采用LARS人工韧带重建后交叉韧带术属于微创手术．避免了采用自体肌腱重建须取材的并发症,也避免了同种异体移植材料的排斥及传染疾病的并发症。其手术操作简便,手术时间短,术后关节恢复快。     

关节镜下单根LARS人工韧带重建后交叉韧带及内侧副韧带短期临床疗效观察

目的观察后交叉韧带(posterior cruciate ligament,PCL)、内侧副韧带(medial collateral ligament,MCL)同时损伤后,应用LARS人工韧带关节镜下重建PCL,并同时利用残余LARS人工韧带重建MCL的短期临床疗效。方法对8例关节损伤的患者在关节镜下行LARS人工韧带重建PCL,同时利用残余人工韧带重建MCL,术后随访6个月,比较术前、术后膝关节Lysholm评分和稳定性。结果 8例患者术前物理检查均表现为膝关节不稳,术后所有患膝不稳定表现消失。术后无膝关节感染、韧带自发断裂和韧带松动并发症,屈膝活动度110°～120°,术后1、3、6个月膝关节Lysholm评分均较术前增高,差异具有统计学意义(P<0.05)。结论应用LARS人工韧带关节镜下重建PCL,同时利用残余人工韧带修复MCL,术后膝关节可获得即时稳定,利于膝关节早期活动,减少相关并发症的发生,短期临床疗效满意。     

LARS人工韧带与自体腘绳肌腱重建前交叉韧带的早期临床疗效比较

目的前瞻性对照研究LARS(ligament advanced reinforcementsystem)人工韧带与四股自体月国绳肌腱重建前交叉韧带(anterior cruciate ligament,ACL)手术的早期临床疗效。方法自2004年8月～2005年6月,共42例前交叉韧带损伤病例,根据LARS韧带使用指征,医生建议和患者意愿,选择自体月国绳肌腱或LARS人工韧带作为移植物。其中LARS韧带重建ACL病例23例,两端以挤压螺钉固定;四股自体月国绳肌腱重建前交叉韧带者19例,股骨端Endobutton悬吊固定,胫骨端Intrafix挤压螺钉和Spiked-washer拴桩双重固定。均在术前及术后3、6、9和15个月给予Lysholm、Tegner、IKDC评分。结果与自体月国绳肌腱重建ACL的病例比较,采用LARS韧带的病例,在术后平均9个月的随访期内,3种膝关节功能评分值高于自体月国绳肌腱A-CL重建的病例。结论应用LARS人工韧带的病例膝关节功能评分均较术前有明显提高。使用LARS人工韧带的病例较使用自体月国绳肌腱的病例术后膝关节功能恢复更早。     

LARS人工韧带与自体月国绳肌腱重建前交叉韧带的早期临床疗效比较

目的前瞻性对照研究LARS(ligament advanced reinforcementsystem)人工韧带与四股自体月国绳肌腱重建前交叉韧带(anterior cruciate ligament,ACL)手术的早期临床疗效。方法自2004年8月～2005年6月,共42例前交叉韧带损伤病例,根据LARS韧带使用指征,医生建议和患者意愿,选择自体月国绳肌腱或LARS人工韧带作为移植物。其中LARS韧带重建ACL病例23例,两端以挤压螺钉固定;四股自体月国绳肌腱重建前交叉韧带者19例,股骨端Endobutton悬吊固定,胫骨端Intrafix挤压螺钉和Spiked-washer拴桩双重固定。均在术前及术后3、6、9和15个月给予Lysholm、Tegner、IKDC评分。结果与自体月国绳肌腱重建ACL的病例比较,采用LARS韧带的病例,在术后平均9个月的随访期内,3种膝关节功能评分值高于自体月国绳肌腱A-CL重建的病例。结论应用LARS人工韧带的病例膝关节功能评分均较术前有明显提高。使用LARS人工韧带的病例较使用自体月国绳肌腱的病例术后膝关节功能恢复更早。     

关节镜下自体腘绳肌腱重建前交叉韧带的临床效果观察

目的:对关节镜下前交叉韧带自体腘绳肌腱重建手术治疗前交叉韧带损伤的临床疗效进行分析探究。方法:选取在我院接受治疗的50例前交叉韧带损伤患者作为本次研究对象,并由同一名术者对其进行关节镜下前交叉韧带自体腘绳肌腱重建手术治疗,对比分析手术治疗前、后患者的膝关节功能评分以及相应指标改善情况,并与传统开放手术治疗前交叉韧带效果相比。结果:手术治疗后1个月患者的双股部周颈差、膝关节活动角度明显优于手术前,差异有统计学意义(P<0.05)。手术治疗后患者的Lysholm膝关节评分与手术前相比明显较高,差异有统计学意义(P<0.05)。关节镜组患者的24h术后引流血量、平均异体输血量以及异体输血率明显优于传统组,差异有统计学意义(P<0.05)。结论:关节镜下前交叉韧带自体腘绳肌腱重建手术治疗前交叉韧带损伤的临床疗效显著,可改善患者膝关节功能,促进膝关节稳定性的恢复,增加膝关节的活动程度,临床意义极其重大。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.